Diagnostic Accuracy of Cancer Antigen 15-3 as a Seromarker Among Recurrent Breast Carcinoma in Bangladesh.

BACKGROUND: The diagnosis of recurrent breast carcinoma is crucial for patient treatment. The present study aimed to assess the diagnostic accuracy of cancer antigen 15-3 (CA 15-3) as a sero-marker among recurrent breast carcinoma patients. METHODS: This prospective observational study evaluated the serum CA 15-3 among women (age ≥18 years) with recurrent breast carcinoma. The CA 15-3 was measured by the enzyme-linked immunosorbent assay (ELISA), and concentrations were stratified using a cut-off value of 30 U/mL. The receiver operating characteristic (ROC) curve observed that the sensitivity and specificity of the CA 15-3 cut-off value and the area under the AUROC curve demonstrate the goodness-of-fit of the prediction model. RESULTS: A total of 50 patients were recruited, with a mean age of 48.4 ±9.7years. The majority (n=28, 56.0%) of patients were 41 to 50 years old. Further, a total of 42 (84%) patients had high serum levels of CA 15-3, with a mean value of 72.7±9.5 U/mL. At the cut-off level of 30 U/mL, the ROC curve demonstrated sensitivity, specificity, positive predictive value, and negative predictive value of 95.7%, 69.4%, 84.1%, and 72.8%, respectively, to diagnose recurrent breast carcinoma. Nonetheless, the area under the ROC (AUROC) curve was 0.712, indicating a satisfactory fit for the prediction model. CONCLUSION: We found that CA 15-3 level ≥30 U/mL is highly sensitive and specific as a seromarker for detecting recurrent breast cancer among the Bangladeshi population. We recommend routinely monitoring breast cancer survivors using CA 15-3 biomarkers.

A Synergistic Overview between Microfluidics and Numerical Research for Vascular Flow and Pathological Investigations.

Vascular diseases are widespread, and sometimes such life-threatening medical disorders cause abnormal blood flow, blood particle damage, changes to flow dynamics, restricted blood flow, and other adverse effects. The study of vascular flow is crucial in clinical practice because it can shed light on the causes of stenosis, aneurysm, blood cancer, and many other such diseases, and guide the development of novel treatments and interventions. Microfluidics and computational fluid dynamics (CFDs) are two of the most promising new tools for investigating these phenomena. When compared to conventional experimental methods, microfluidics offers many benefits, including lower costs, smaller sample quantities, and increased control over fluid flow and parameters. In this paper, we address the strengths and weaknesses of computational and experimental approaches utilizing microfluidic devices to investigate the rheological properties of blood, the forces of action causing diseases related to cardiology, provide an overview of the models and methodologies of experiments, and the fabrication of devices utilized in these types of research, and portray the results achieved and their applications. We also discuss how these results can inform clinical practice and where future research should go. Overall, it provides insights into why a combination of both CFDs, and experimental methods can give even more detailed information on disease mechanisms recreated on a microfluidic platform, replicating the original biological system and aiding in developing the device or chip itself.

Health hazardous index based trace metals and essential acids analysis of size-dependent market available Hilsa fish, Bangladesh: Experimental and chemometric approaches.

With priority given to various-sized samples of market-available Hilsa (Tenualosa ilisha), human health consequences of trace metals along with total essential acids, including the fatty acid (FA) and amino acid (AA) profile were measured and compared to different size groups (G I, G II, and G III) using chemometric approaches. Essential amino acids were lower than nonessential amino acids. The G III contained the highest (97.55%) saturated and unsaturated fatty acids. The highest concentrated metal was found in G1 among the groups and the order of metal (mg/kg) was Zn (205.01) > Mn (37.37) > Fe (69.39) > Cu (1.47) > Cr (1.31) > Ni (0.42) > Pb (0.017) > Cd (0.005). Even though the adult group showed no health hazards for Hilsa consumption, non-carcinogenic risks have been identified for G1 fish consumption by children. Continued monitoring is recommended to overcome the health consequences caused by fish consumption.

Design, Synthesis, and Evaluation of p53Y220C Acetylation Targeting Chimeras (AceTACs).

The well-known tumor suppressor p53 is mutated in approximately half of all cancers. The Y220C mutation is one of the major p53 hotspot mutations. Several small-molecule stabilizers of p53Y220C have been developed. We recently developed a new technology for inducing targeted protein acetylation, termed acetylation targeting chimera (AceTAC), and the first p53Y220C AceTAC that effectively acetylated p53Y220C at lysine 382. Here, we report structure-activity relationship (SAR) studies of p53Y220C AceTACs, which led to the discovery of a novel p53Y220C AceTAC, compound 11 (MS182). 11 effectively acetylated p53Y220C at lysine 382 in a time- and concentration-dependent manner via inducing the ternary complex formation between p300/CBP acetyltransferase and p53Y220C. 11 was more effective than the parent p53Y220C stabilizer in suppressing the proliferation and clonogenicity in cancer cells harboring the p53Y200C mutation and was bioavailable in mice. Overall, 11 is a potentially valuable chemical tool to investigate the role of p53Y220C acetylation in cancer.

Exploration and characterization of a newly isolated bacterium, Enterobacter quasihormaechei strain BDIFST24001, capable of producing rhamnolipid biosurfactant for oil remediation.

Biosurfactants are naturally occurring compounds synthesized by micro-organisms that increasingly attract attention due to both their living area and application in various industries. In this study, we explore and characterize a novel bacterium, Enterobacter quasihormaechei strain BDIFST24001, isolated for its ability to produce rhamnolipid biosurfactants, with the aim of facilitating oil remediation processes. The isolation of this bacterium was carried out using Luria-Bertani (LB) broth media from environmental samples collected from oil-contaminated sites in Dhaka City. Screening tests, including the oil spreading method and drop collapse assay, were conducted to identify potential biosurfactant-producing strains, leading to the selection of E. quasihormaechei strain BDIFST24001 based on its favourable performance. Subsequent molecular identification revealed a high similarity of the strain's 16S rRNA gene to E. quasihormaechei, which was corroborated through phylogenetic analysis. Further analysis of the biosurfactant produced by this strain indicated its rhamnolipid nature, as confirmed by FT-IR spectroscopy. The rhamnolipids exhibited promising surface-active properties, including a significant reduction in surface tension and emulsification activity, as evidenced by surface tension measurements and emulsification index assays. Optimization studies revealed that the optimal conditions for rhamnolipid production by E. quasihormaechei strain BDIFST24001 were a temperature of 37 °C, pH 10.0 and salinity of 4 %. The rhamnolipids produced by this strain demonstrated effective oil remediation capabilities, as observed through controlled experiments using petrol oil. The rhamnolipids effectively reduced the surface tension of the oil-water interface, facilitating the dispersion and emulsification of the oil phase in water. Overall, our findings highlight the potential of E. quasihormaechei strain BDIFST24001 as a promising candidate for biosurfactant-mediated oil spill cleanup and environmental remediation efforts.

Exploring the nutraceutical potential: Evaluating the nutritional and bioactive functions of five pomelo fruit varieties in Bangladesh.

Pomelo (Citrus maxima), the largest citrus fruit, provides a variety of nutrients that have several health benefits, including antioxidant and antidiabetic functions. Antioxidants help combat oxidative stress by neutralizing reactive oxygen species (ROS) and reducing cellular damage. On the other hand, antidiabetic properties involve mechanisms such as enhancing insulin secretion, improving insulin sensitivity, inhibiting carbohydrate digestion and absorption, and regulating glucose metabolism. However, there is a lack of data on the comparative analysis of the physicochemical composition, bioactive properties, and antidiabetic effects of pomelo fruits grown in Bangladesh. To address this issue, the most common and popular high-yielding five cultivars of pomelo fruits grown in Bangladesh including LOCAL, BARI-2 (BARI: Bangladesh Agricultural Research Institute, Batabi Lebu-2), BARI-3, BARI-4, and BARI-6 were evaluated concerning proximate, minerals, and physicochemical properties with their antidiabetic and antioxidant properties. Research has revealed that all pomelo varieties contained a significant amount of proximate compositions and major minerals (Ca, Mg, K, Na, and Fe). The highest juice yield (75.37 ± 0.33 %), vitamin C content (79.56 ± 2.26 mg/100 mL of fresh juice), and carotenoid content (919.33 ± 0.62 µM ß-Carotene Equivalent/g DM) were found in BARI-3 pomelo fruit and adhered to the sequence (p < 0.05): BARI-3 > LOCAL > BARI-4 > BARI-6 > BARI-2; BARI-3 > LOCAL > BARI-2 > BARI-4 > BARI-6, and BARI-3 > BARI-2 > BARI-6 > LOCAL > BARI-4, respectively. The anthocyanin content and inhibitory activity of α-glucosidase were found to be at their peak in the BARI-2 pomelo variety and the values were 50.65 ± 2.27 µg cyanidin 3-glucoside equivalents/100 g DM and 85.57 ± 0.00 µM acarbose equivalents/g DM, respectively. BARI-3 pomelo variety showed highest DPPH antioxidant capacity (170.47 ± 0.01 µM Trolox equivalents/g DM), while the BARI-6 pomelo variety exhibited the highest total phenolic content (6712.30 ± 1.84 µg gallic acid equivalents/g DM), and ferric-reducing antioxidant power activity (183.16 ± 0.01 µM Fe(II) equivalents/g DM). Therefore, this study explores the nutritional value and bioactivity of five popular pomelo varieties in Bangladesh, offering valuable insights for utilizing high-value citrus resources and understanding their health-promoting functions.

Comparing effectiveness of physiotherapy versus drug management on fatigue, physical functioning, and episodic disability for myalgic encephalomyelitis in post-COVID-19 condition: a study protocol of randomized control trial.

BACKGROUND: Physiotherapy interventions effectively improved fatigue and physical functioning in non-COVID patients with myalgic encephalomyelitis or chronic fatigue syndrome (ME/CFS). There is a research gap on the effectiveness of physiotherapy interventions versus drug management on ME/CFS in post-COVID-19 conditions (PCC). METHODS: We planned a three-arm prospective randomized control trial on 135 PCC cases with ME/CFS who are diagnosed between 20 November 2023 and 20 May 2024 from a population-based cohort. The study aims to determine the effectiveness of physiotherapy interventions as adapted physical activity and therapeutic exercise (APTE) provided in institution-based care versus telemedicine compared with drug management (DM). Participants will be assigned to three groups with the concealed location process and block randomization with an enrollment ratio of 1:1:1. The post-treatment evaluation will be employed after 2 months of interventions, and follow-up will be taken after 6 months post-intervention. The Chalder fatigue scale will measure the primary outcome of fatigue. SF-36 and the disability-adjusted life years (DALYs) will measure the secondary outcome of physical functioning and episodic disability. DISCUSSION: This study will address the research gap to determine the appropriate approach of physiotherapy or drug management for ME/CFS in PCC cases. The future direction of the study will contribute to developing evidence-based practice in post-COVID-19 condition rehabilitation. TRIAL REGISTRATION: The trial is registered prospectively from a primary Clinical Trial Registry side of WHO CTRI/2024/01/061987. Registered on 29 January 2024.

Explainable machine learning approach for cancer prediction through binarilization of RNA sequencing data.

In recent years, researchers have proven the effectiveness and speediness of machine learning-based cancer diagnosis models. However, it is difficult to explain the results generated by machine learning models, especially ones that utilized complex high-dimensional data like RNA sequencing data. In this study, we propose the binarilization technique as a novel way to treat RNA sequencing data and used it to construct explainable cancer prediction models. We tested our proposed data processing technique on five different models, namely neural network, random forest, xgboost, support vector machine, and decision tree, using four cancer datasets collected from the National Cancer Institute Genomic Data Commons. Since our datasets are imbalanced, we evaluated the performance of all models using metrics designed for imbalance performance like geometric mean, Matthews correlation coefficient, F-Measure, and area under the receiver operating characteristic curve. Our approach showed comparative performance while relying on less features. Additionally, we demonstrated that data binarilization offers higher explainability by revealing how each feature affects the prediction. These results demonstrate the potential of data binarilization technique in improving the performance and explainability of RNA sequencing based cancer prediction models.

Discovery of the First-in-Class G9a/GLP PROTAC Degrader.

Aberrantly expressed lysine methyltransferases G9a and GLP, which catalyze mono- and dimethylation of histone H3 lysine 9 (H3K9), have been implicated in numerous cancers. Recent studies have uncovered both catalytic and noncatalytic oncogenic functions of G9a/GLP. As such, G9a/GLP catalytic inhibitors have displayed limited anticancer activity. Here, we report the discovery of the first-in-class G9a/GLP proteolysis targeting chimera (PROTAC) degrader 10 (MS8709), as a potential anticancer therapeutic. 10 induces G9a/GLP degradation in a concentration-, time-, and ubiquitin-proteasome system (UPS)-dependent manner. Futhermore, 10 does not alter the mRNA expression of G9a/GLP and is selective for G9a/GLP over other methyltransferases. Moreover, 10 displays superior cell growth inhibition to the parent G9a/GLP inhibitor UNC0642 in prostate, leukemia, and lung cancer cells and has suitable mouse pharmacokinetic properties for in vivo efficacy studies. Overall, 10 is a valuable chemical biology tool to further investigate the functions of G9a/GLP and a potential therapeutic for treating G9a/GLP-dependent cancers.

Discovery and Characterization of a Novel Cereblon-Recruiting PRC1 Bridged PROTAC Degrader.

Bridged PROTAC is a novel protein complex degrader strategy that exploits the target protein's binding partner to degrade undruggable proteins by inducing proximity to an E3 ubiquitin ligase. In this study, we discovered for the first time that cereblon (CRBN) can be employed for the bridged PROTAC approach and report the first-in-class CRBN-recruiting and EED-binding polycomb repressive complex 1 (PRC1) degrader, compound 1 (MS181). We show that 1 induces preferential degradation of PRC1 components, BMI1 and RING1B, in an EED-, CRBN-, and ubiquitin-proteosome system (UPS)-dependent manner. Compound 1 also has superior antiproliferative activity in multiple metastatic cancer cell lines over EED-binding PRC2 degraders and can be efficacious in VHL-defective cancer cells. Altogether, compound 1 is a valuable chemical biology tool to study the role of PRC1 in cancer. Importantly, we show that CRBN can be utilized to develop bridged PROTACs, expanding the bridged PROTAC technology for degrading undruggable proteins.

RETRACTED: Nobel et al. Modern Subtype Classification and Outlier Detection Using the Attention Embedder to Transform Ovarian Cancer Diagnosis. Tomography 2024, 10, 105-132.

The Tomography Editorial Office retracts the article "Modern Subtype Classification and Outlier Detection Using the Attention Embedder to Transform Ovarian Cancer Diagnosis" [...].

Functional Evaluation of Fermented Rice Bran and Extracted Rice Bran Oil Addressing for Human Health Benefit.

Rice bran (RB) and rice bran oil (RBO) are exploring as prominent food component worldwide and their compositional variation is being varied among the world due to regional and production process. In this study, Fermented Rice Bran (FRB) was produced by employing edible gram-positive bacteria (Lactobacillus acidophilus, Lactobacillus bulgaricus and Bifidobacterium bifidum) at 125×10 5 spore g -1 of rice bran, and investigated to evaluate nutritional quality. The Crude Rice Bran Oil (CRBO) was extracted from RB and its quality was also investigated compared to market available rice bran oil (MRBO) in Bangladesh. We found that fermentation of rice bran with lactic acid bacteria increased total proteins (29.52%), fat (5.38%), ash (48.47%), crude fiber (38.96%), and moisture (61.04%) and reduced the carbohydrate content (36.61%). We also found that essential amino acids (Threonine, valine, leucine, lysine, histidine and phenylalanine) and non-essential amino acids (alanine, aspartate, glycine, glutamine, serine and tyrosine) were increased in FRB except methionine and proline. Moreover, total phenolic content, tannin content, flavonoid content and antioxidant activity were increased in FRB. The RBO analysis showed that γ-oryzanol content (10.00 mg/g) were found in CRBO compared to MRBO (ranging 7.40 to 12.70 mg/g) and Vitamin-E content 0.20% were found higher in CRBO compared to MRBO (ranging 0.097 to 0.12%). The total saturated (25.16%) and total unsaturated fatty acids (74.44%) were found in CRBO whereas MRBO contained total saturated (22.08 to 24.13%) and total unsaturated fatty acids (71.91 to 83.29%) respectively. The physiochemical parameters (density, refractive index, iodine value) were found satisfactory in all sample except acid value and peroxide value higher in CRBO. Heavy metal concentration was found within an acceptable range in both CRBO and MRBO. Thus FRB and RBO could be value added food supplement for human health.

Discovery of the First-in-class G9a/GLP PROTAC Degrader.

Aberrantly expressed lysine methyltransferases G9a and GLP, which catalyze mono- and di-methylation of histone H3 lysine 9 (H3K9), have been implicated in numerous cancers. Recent studies have uncovered both catalytic and non-catalytic oncogenic functions of G9a/GLP. As such, G9a/GLP catalytic inhibitors have displayed limited anticancer activity. Here, we report the discovery of the first-in-class G9a/GLP proteolysis targeting chimera (PROTAC) degrader, 10 (MS8709), as a potential anticancer therapeutic. 10 induces G9a/GLP degradation in a concentration-, time, and ubiquitin-proteasome system (UPS)-dependent manner, does not alter the mRNA expression of G9a/GLP and is selective for G9a/GLP over other methyltransferases. Moreover, 10 displays superior cell growth inhibition to the parent G9a/GLP inhibitor UNC0642 in prostate, leukemia, and lung cancer cells and has suitable mouse pharmacokinetic properties for in vivo efficacy studies. Overall, 10 is a valuable chemical biology tool to further investigate the functions of G9a/GLP and a potential therapeutic for treating G9a/GLP-dependent cancers.

Comparative evaluation of physicochemical and antimicrobial properties of rubber seed oil from different regions of Bangladesh.

In Bangladesh, the annual production of rubber seeds is typically left untapped although the seeds contained a high percentage of oil but underutilized without any value-added utilization. This study aims to evaluate the geographical effect on physicochemical properties, fatty acid composition and the antimicrobial activity of oil extracted from rubber seeds. Seeds were collected from three different regions of Bangladesh and the oil was extracted by the soxhlet method using n-hexane as a solvent. Results demonstrated that the geographical regions have some significant effect on the properties of rubber seed oil (RSO). The physicochemical properties of RSO varied from region to region. For example, the percent of yield, higher heating value, and flash point varied from 50.0 to 50.8 %, 31.8-33.3 kJ/g, and 237-245 °C, respectively. The chemical parameters, such as acid value, iodine value, and hydroxyl value varied from 13.3 to 18.2 mg KOH/g, 132-137 g I2/100g, and 47.7-55.8 mg KOH/g, respectively. Chromatographic analysis showed that RSO mainly contains palmitic, linoleic, linolenic, and stearic acid. Regional variations were also seen in the composition of these fatty acids. Most notably, regardless of the rubber seeds collected from various locations, RSO exhibited inhibitory activity against only gram positive bacteria. The zone of inhibition range for different tested gram positive bacteria was 2.33-11.17 mm irrespective of different RSO samples.

Method development of multi pesticide residue analysis in country beans collected from Dhaka, Bangladesh, and their dietary risk assessment.

The aim of the study was to develop a modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of five multi-class pesticides in country beans collected from Dhaka, Bangladesh. Pesticides were extracted using ACN, and to minimize the co-extraction matrix, optimized d-SPE cleanup was done using sorbents (GCB, PSA, and C18). In the calibration range, the method showed excellent linearity with a correlation coefficient of R2 ≥ 0.9990 both in solvent- and matrix-matched calibration. For the selected pesticides, average recoveries (at four spiking levels (n = 5) of 10, 20, 100, and 200 µg/kg) of 70-100 % were achieved with relative standard deviations (RSDs) ≤ 9.5 %. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.3333 to 1.3333 µg/kg and 1.0 to 4.0 µg/kg, respectively. The dietary risk assessment, in terms of hazard quotient (HQ), was calculated to assess consumers' health risks.

Modern Subtype Classification and Outlier Detection Using the Attention Embedder to Transform Ovarian Cancer Diagnosis.

Ovarian cancer, a deadly female reproductive system disease, is a significant challenge in medical research due to its notorious lethality. Addressing ovarian cancer in the current medical landscape has become more complex than ever. This research explores the complex field of Ovarian Cancer Subtype Classification and the crucial task of Outlier Detection, driven by a progressive automated system, as the need to fight this unforgiving illness becomes critical. This study primarily uses a unique dataset painstakingly selected from 20 esteemed medical institutes. The dataset includes a wide range of images, such as tissue microarray (TMA) images at 40× magnification and whole-slide images (WSI) at 20× magnification. The research is fully committed to identifying abnormalities within this complex environment, going beyond the classification of subtypes of ovarian cancer. We proposed a new Attention Embedder, a state-of-the-art model with effective results in ovarian cancer subtype classification and outlier detection. Using images magnified WSI, the model demonstrated an astonishing 96.42% training accuracy and 95.10% validation accuracy. Similarly, with images magnified via a TMA, the model performed well, obtaining a validation accuracy of 94.90% and a training accuracy of 93.45%. Our fine-tuned hyperparameter testing resulted in exceptional performance on independent images. At 20× magnification, we achieved an accuracy of 93.56%. Even at 40× magnification, our testing accuracy remained high, at 91.37%. This study highlights how machine learning can revolutionize the medical field's ability to classify ovarian cancer subtypes and identify outliers, giving doctors a valuable tool to lessen the severe effects of the disease. Adopting this novel method is likely to improve the practice of medicine and give people living with ovarian cancer worldwide hope.

Ultrafast and simultaneous removal of four tetracyclines from aqueous solutions using waste material-derived graphene oxide-supported cobalt-iron magnetic nanocomposites.

In this work, a graphene oxide-supported cobalt-iron oxide (GO/Co-Fe) magnetic nanocomposite was successfully synthesized using waste dry cells for the efficient and simultaneous removal of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC), and doxycycline (DTC) from aqueous solutions. The GO/Co-Fe nanocomposite was thoroughly characterized using Fourier transform infrared spectroscopy, vibrating sample magnetometry, X-ray diffraction, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and zeta potential analysis. This multi-faceted characterization provided clean insights into the composition and properties of the synthesized nanocomposite. The adsorption of tetracyclines (TCs) was systematically investigated by assessing the influence of critical factors, such as adsorbent dosage, contact duration, initial pH of the solution, initial concentration, and temperature. The GO/Co-Fe adsorbent showed high removal efficiencies of 94.1% TC, 94.32% CTC, 94.22% OTC, and 96.94% DTC within 30 s contact period. The maximum removal efficiency of TCs was found at a low adsorbent dose of 0.15 g L-1. Notably, this superior removal efficiency was achieved at neutral pH and room temperature, demonstrating the adsorbent's efficacy under environmentally viable conditions. The kinetic studies demonstrated that the adsorption process was fitted satisfactorily with the pseudo-second-order model. Additionally, the adsorption behaviour of TCs on the GO/Co-Fe adsorbent was assessed by isotherm models, Langmuir and Freundlich. The experimental data followed the Langmuir isotherm, signifying a monolayer adsorption mechanism on the surface of the adsorbent. The adsorption capacities (qm) of GO/Co-Fe for TC, CTC, OTC and DTC were determined to be 64.10, 71.43, 72.46 and 99.01 mg g-1, respectively. Importantly, the GO/Co-Fe adsorbent showed reusability capabilities. The super magnetic properties of GO/Co-Fe made it easy to use for several cycles. These results clearly establish GO/Co-Fe as an exceptionally effective adsorbent for the removal of TCs from aqueous systems, highlighting its great potentiality in water treatment applications.

Development of functional noodles by encapsulating mango peel powder as a source of bioactive compounds.

Antioxidant compounds such as phenolics and carotenoids scavenge reactive oxygen species and protect against degenerative diseases such as cancer and cardiovascular disease when used as food additives or supplements. Mango peel is a by-product of mango which is a good source of bioactive substances such as phytochemicals, antioxidants, and dietary fibers. Unfortunately, the study on mango peel as a potential food additive is very limited. Accordingly, the present study aimed to develop functional noodles through extrusion technology with the encapsulation of mango peel powder as a natural source of bioactive compounds. First, mango peel powder (MPP) was prepared and incorporated during the mixing of ingredients before noodles formation at three different levels (2.5, 5 and 7.5 %). Afterward, the noodles were studied to determine how the encapsulated MPP affects the proximate composition, physicochemical characteristics, polyphenols, carotenoids, anthocyanin, antioxidant and antidiabetic activity, and sensory characteristics. The noodles exhibited a dose-dependent relationship in the content of bioactive components and functional activities with the encapsulation of MPP levels. A significantly (p 0.05) higher value was noticed in 7.5 % of MPP-encapsulated noodles than in any level of MPP encapsulation in noodles. The fiber and protein contents in the MPP-encapsulated noodles were increased by about 0-1.22 % and 0-3.16 %, respectively. However, noodles' color index and water absorption index were decreased with the level of MPP encapsulation. The cooking loss of noodles increased from 4.64 to 5.17, 6.49, and 7.32 %, whereas the cooked weight decreased from 35.11 to 34.40, 33.65, and 33.23 % with 2.5, 5.0, and 7.5 % of MPP encapsulation, respectively. However, MPP was stable during storage of noodles exhibiting higher phenolic content and antioxidant activity than control samples. The sensory evaluation showed that MPP-encapsulated noodles at levels 2.5 and 5 % had approximately similar overall acceptability values with the control sample. As a result of the findings, it appears that adding MPP up to 5 % to noodles improves their nutritional quality without changing their cooking, structural, or sensory aspects. Therefore, mango peel powder can be a potential cheap source for the development of functional noodles and food ingredients.

Impaired intestinal immunity and microbial diversity in common carp exposed to cadmium.

Waterborne cadmium (Cd) accumulates in the fish intestine and causes irreversible toxicity by disrupting intestinal immunity and microbial diversity. To explore the toxicity of environmentally available high Cd concentration on intestinal immunity and microbial diversity of fish, we selected the widely used bioindicator model species, Common carp (Cyprinus carpio). Literature review and Cd pollution data supported sequential doses of 0.2, 0.4, 0.8, 1.6, 3.2, and 6.4 mg/L Cd for 30 days. Based on intestinal tissue Cd accumulation, previous studies, and environmentally available Cd data, 0.4 and 1.6 mg/L Cd were selected for further studies. Intestinal Cd bioaccumulation increased significantly to ~100 times in fish exposed to 1.6 mg/L Cd. We observed villous atrophy, increased goblet cells with mucus production, muscularis erosion, and thickened lamina propria due to intense inflammatory cell infiltration in the intestine at this Cd concentration. Cd-induced immunosuppression occurred with increased lysozyme, alkaline phosphate (AKP), and acid phosphate (ACP). High levels of catalase (CAT), total antioxidant capacity (T-AOC), malondialdehyde (MDA), and hydrogen peroxide (H2O2) suggested induced oxidative stress and poor metabolism by α-amylase and lipase suppression for Cd toxicity. Proteobacteria (41.2 %), Firmicutes (21.8 %), and Bacteroidetes (17.5 %) were the dominant bacterial phyla in the common carp intestine. Additionally, potential pathogenic Cyanobacteria increased in Cd-treated fish. The decrease of beneficiary bacteria like Aeromonas, and Cetobacterium indicated Cd toxicity. Overall, these findings indicate harmful consequences of high Cd concentration in the intestinal homeostasis and health status of fish.

Morphometric features, seminal profile and diluters effect on post-thaw semen quality of Munshiganj cattle in Bangladesh.

The study investigates morphometric features, seminal profile and post-thaw semen quality of Munshiganj cattle. Morphometric features were measured using measuring tape from 20 Munshiganj cattle while coat color was measured by observing in naked eye. Fresh and post thaw semen quality parameters were analyzed using Computer Assisted semen analyzer (CASA). Coat color of Munshiganj male cattle were creamy white to dull pinkish and female were white to creamy. The mean body weight, body length, hearth girth, height at wither, head length, head width, ear length, ear width, fore leg length, hind leg length, tail length, tail doc circumference, horn length, horn diameter and mouth circumference were 362.80 kg, 137.31 cm, 160.66 cm, 135.21 cm, 50.97 cm, 20.58 cm, 19.75 cm, 9.88 cm, 73.02 cm, 74.84 cm, 106.10 cm, 20.75 cm, 13.60 cm, 16.12 cm and 43.00 cm, respectively. There was significant difference (p < 0.05) between male and female in terms of body weight (418.00 vs 307.60 kg), heart girth (173.74 vs 147.57 cm), head width (22.50 vs 18.67 cm), horn diameter (18.58 vs 13.66 cm) and mouth circumference (46.60 vs 39.40 cm). Average scrotal length was 16.76 cm while scrotal circumference was 32.70 cm. Age had significant effect (p < 0.05) on morphometric characteristics of Munshiganj male and female cattle. On the other hand, season had no significant effect on semen quality. Mean semen volume, sperm concentration, total motile, progressive, static, slow and live spermatozoa were 5.83 ± 0.88 ml, 1510.27 ± 844.07 million/ml, 91.9 ± 2.17 %, 63.80 ± 12.53 %, 8.10 ± 2.17 %, 0.10 ± 0.10 %, 91.38 ± 0.25 %, respectively. On the other hand, sperm head length and width, sperm tail length of Munshiganj cattle were 10.39 ± 0.16 µm, 4.26 ± 0.07 µm, 21.5 ± 0.52 µm, respectively. Individual breeding bull had a significant (p < 0.05) effect on post-thawed motile sperm percentage. Four different diluters (Triladyl, Steridyl, Tris-egg yolk-Citrate and Andromed) were used to compare the effects of diluter on post-thaw semen quality of Munshiganj cattle and found that diluter had no significant effect (p > 0.05) on post thaw semen quality except slow motility and proximal droplet percentages. Munshiganj cattle had a distinctive phenotypic feature with standard quality semen and had no effect of egg yolk free and egg yolk based diluters on post thaw semen quality.