Light microscopic morphology of blood cells of non-descript indigenous Zoar chicken of Mizoram, India.

Identifying and analysing distinct blood cells is crucial for the diagnosis and treatment of diseases in the field of biomedicine. The present study was undertaken to study the cytomorphological and cytochemical characteristics of the blood cells of Zoar, a non-descript indigenous breed of chicken extensively reared under backyard poultry farming in Mizoram, India. For this study, 2 mL of blood samples were aseptically collected from the wings veins of 12 chickens and were processed for light microscopic study under standard protocols. The matured erythrocytes were elliptical, while the immature erythrocytes appeared oval. The heterophils were positive for SBB (SBB), Periodic Acid Schiff (PAS), acid phosphatase, alkaline phosphatase and Arylsulphatase while the eosinophils were positive for SBB, PAS, alkaline phosphatase, cytochrome oxidase and peroxidase. The basophils of were positive for toluidine blue while the thrombocytes were positive for PAS. These cytochemical and cytoenzymatic staining properties plays a very important role in diagnosis, differentiation, and classification of leukaemias.

Observations on the cytomorphology and ultrastructure of the peripheral blood cells of native cattle (Zobawng) of Mizoram, India.

This study aims to characterize the cytomorphology and ultrastructure of blood cells of native cattle of Mizoram. Twelve numbers of blood samples (10 ml) were collected from the Zobawng cattle, irrespective of sex. Blood smears were prepared and stained with different stains for cytomorphological study. The standard protocol has been followed for preparing blood samples for electron microscopy. Under a light microscope, erythrocytes of cattle were non-nucleated and round. The neutrophils were round, and the cytoplasm contained cytoplasmic granules. The eosinophils were rounded in outline with distinct cytoplasmic granules. The presence of basophils was infrequent with distinct blue color cytoplasmic granules. Small, medium, and large types of lymphocytes were recorded. The monocytes were round to oval in outline. Platelets were irregular to round. The reticulocytes were recorded occasionally, like small blue thin rods or granules. The cytoplasm and nucleus of granulocytes fluoresced greenish-yellow and red, respectively, with supravital stain. Under scanning electron microscopy (SEM), the erythrocytes appeared as biconcave discs. Different leukocytes were observed with their finger-like, plate-like, and narrow cell processes on their surface. Platelets were irregular structures. In transmission electron microscopy (TEM), the erythrocytes appeared anucleated biconcave elongated, neutrophils were roughly rounded with small cytoplasmic processes, and eosinophils were roughly circular with small cytoplasmic processes, the basophils were roughly circular with oval to elongated cytoplasmic granules, lymphocytes were roughly circular with centrally placed well-marked oval indented nucleus and some cytoplasmic processes, monocytes appeared spherical with long thick cytoplasmic processes and the non-nucleated platelets appeared roughly round to elongated. RESEARCH HIGHLIGHTS: Blood tests are a very good tool for determining animal health and diagnosing hematological illnesses. This study showed the cytomorphological and ultrastructural features of the blood cells of native cattle (Zobawng) of Mizoram state of India. The cytomorphological studies revealed that the morphological features of the blood cells of Zobawng cattle resembled to other domestic and wild animals, however, there were some differences in the cellular components. Nuclear DNA appeared orthochromatically greenish-yellow, but cytoplasmic RNA was observed to be metachromatically red under the fluorescence microscope. scanning electron microscopic (SEM) and transmission electron microscopic (TEM) results revealed that the ultrastructural features of the blood cells of Zobawng cattle contain certain cellular morphological differences as compared to the other domestic and wild animals.

Morphological and Applied Anatomical Studies on the Head Region of Local Mizo Pig (Zovawk) of Mizoram / Estudios Morfológicos y Anatómicos Aplicados en la Cabeza de Cerdo Local Mizo (Zovawk) de Mizoram

SUMMARY: Zovawk is newly identified breed of pig of Mizoram approved by breed registration committee of Indian Council of Agricultural Research (ICAR), New Delhi. This study was designed to provide maximum number of morphometrical parameters of skull and some valuable information on clinically important parameters of Zovawk. The skull of the Zovawk was dolichocephalic according to the cephalic index (53.56±0.11). The supraorbital foramina distance, infraorbital foramina distance, skull length, skull width, cranial length and nasal length of the Zovawk were 3.49±0.01 cm, 6.55±0.01 cm, 28.26±0.03 cm, 15.11±0.26 cm, 13.17±0.04 cm and 13.79±0.02 cm, respectively. The orbital margins were found to be incomplete with bilateral variation among the orbits of both the sides. The distance from the process of alveolar socket of canine tooth to the infraorbital canal and from the latter to the root of the fourth upper premolar alveolar tooth directly ventral to it was 4.77±0.04 cm and 1.20±0.01 cm, respectively in Zovawk. The data are of clinical importance as a guide for tracking the infra-orbital nerve, and necessary for its desensitization during the manipulations in the skin of the upper lip, nostril and face at the level of the foramen. The distance between the lateral end of the alveolus of the third incisor tooth to the mental foramen was 3.57±0.04 cm in Zovawk, which is an important landmark for achieving the location of the mental nerve for the regional nerve block Zovawk. The length and height of the mandible were 25.02±0.09 cm and 10.54±0.07 cm, respectively in Zovawk. The morphometric measurements of the skull and applied anatomy of the head region of the Zovawk provide an important baseline data for further research in the field of applied anatomy.

RESUMEN: Zovawk es una raza de cerdo de Mizoram recientemente identificada, aprobada por el comité de registro de razas del Consejo Indio de Investigación Agrícola (ICAR), Nueva Delhi. Este estudio fue diseñado para proporcionar el número máximo de parámetros morfométricos del cráneo y cierta información valiosa sobre parámetros clínicamente importantes de Zovawk. El cráneo de Zovawk es dolicocefálico según el índice cefálico (53,56 ± 0,11). La distancia de foramina supraorbital, la distancia de foramina infraorbital, la longitud del cráneo, el ancho del cráneo, la longitud craneal y la longitud nasal del Zovawk fueron 3.49 ± 0.01 cm, 6.55 ± 0.01 cm, 28.26 ± 0.03 cm, 15.11 ± 0.26 cm, 13.17 ± 0.04 cm y 13.79 ± 0,02 cm, respectivamente. Se encontró que los márgenes orbitales estaban incompletos con una variación bilateral entre las órbitas de ambos lados. La distancia desde el proceso de la cavidad alveolar del diente canino al canal infraorbitario y desde este último a la raíz del cuarto diente alveolar premolar superior directamente ventral fue de 4.77 ± 0.04 cm y 1.20 ± 0.01 cm, respectivamente, en Zovawk. Los datos son de importancia clínica como guía para el seguimiento del nervio infra-orbital y necesarios para su desensibilización durante las manipulaciones en la piel del labio superior, fosa nasal y cara a nivel del foramen. La distancia entre el extremo lateral del alvéolo del tercer diente incisivo y el foramen mental fue de 3.57 ± 0.04 cm en Zovawk, que es un hito importante para lograr la ubicación del nervio mental para el bloqueo nervioso regional Zovawk. La longitud y la altura de la mandíbula fueron 25.02 ± 0.09 cm y 10.54 ± 0.07 cm, respectivamente, en Zovawk. Las mediciones morfométricas del cráneo y la anatomía aplicada de la región de la cabeza de Zovawk proporcionan datos de referencia importantes para futuras investigaciones en el campo de la anatomía aplicada.