[Oncotropic viruses and antitumor autovaccines (review)].

Oncotropic viruses and antitumor autovaccines are reviewed. The progress of tumor process is associated with the invasive growth and the formation of metastases. Nowadays it is obvious that micrometastases are formed before any clinical symptoms are revealed, and, therefore, at the stage of clinical manifestation neoplastic process is in fact a systemic pathology and the surgical intervention is quite inefficient. This work deals with the main mechanisms of the progress of neoplastically transformed cells. Special account is given to the processes and methods which determine the antiblastomic effectiveness of the systemic influence of oncotropic viruses and antitumor autovaccines. The vaccine generated from the autologous tumor cells is actually quite individual. Тechnological procedure requires that the tissues should be removed from different places (primary base, metastases, lymph nodes), otherwise, the immune response to one or several determinants associated with tumor will not block the developing process, but it will promote the selective growth of subclones on the surface of which those determinants are not revealed. Hence, pharmacists face a difficult task - for the purpose of the advancement of the effectiveness of antitumor autovaccine therapy all the blastomic subclones should be infected with oncotropic viruses and additional viral antigens should be formed on their surface. Attenuated strains of natural viruses (flu, measles, herpes) and genetically engineered recombinanted viruses are generally used as oncotherapeutic agents. During the combined viro- and vaccinotherapy it is essentially important to keep the sequence of the following stages: 1) Evaluation of immune status and infection of the organism with oncotropic viruses; 2) Implementation of radical operation before the formation of antiviral immunoglobulins; 3) Removal of tumor tissues from different places ant preparation of autovaccine; 4) Starting the immunization.

[The model specification of the period without recurrences in rats after Pliss lymphosarcoma resection].

The purpose of the present study was to make an experimental model of the period without recurrences in rats after Pliss lymphosarcoma (PLS) resection. Experiments were carried out on white low-bred male rats (n=109). For the tumor transinoculation the rats received subcutaneously 0.2 ml of the aseptic 50% suspension of PLS prepared according to the standards on saline without antibiotic addition. The injections were done in the right abdomen region. The animals were classified into three groups. Group 1 was used for control. An operation of lymphosarcoma resection was carried out on the rats from group 2 on the fifth day from the tumor inoculation. The tumor in the animals from group 3 was dissected on 12-22nd day from its inoculation. At the same time the specification of the duration of the period without recurrences was estimated in every group. According to the suggested method the Pliss lymphosarcoma resection significantly increases the life duration of operated rats that proves the efficiency of surgical interventions. The absence of intraoperative and postoperative lethality on the fifth day from the Pliss lymphosarcoma resection and 100% animal death resulting from the recurrences makes the proposed model potentially suitable for the investigation of adjuvant methods that are used in the treatment of cancer.

[Clomiphene, ovarian hyperstimulation syndrome and pregnancy].

In case of an ovarian hyperstimulation syndrome surgical treatment causes the regress of symptoms much faster than pharmacotherapy--during the resection of an ovary the concentration of estrogenes in blood is effectively reduced. Frequent use of ovulation inductors (Clomiphene Gonadotrop(h)in) is accompanied by ovarian hyperstimulation syndrome. It is characterized by the increase of sizes of ovaries; the formation of ascites and hydrothorax, by the thromboemboly of main blood vessels and etc. Clomiphene accelerates the maturation process of follicles, but contributes to the increase of concentration of oncomarker CA-125 in blood. This makes it difficult to verify the diagnosis of ovary cancer, particularly among pregnants. The case report of infertility treatment with Clomiphene is depicted. Woman became pregnant after three courses of infertility treatment, but pregnancy was complicated with cardiac and lung insufficiency; the suspicion of stage III ovarian cancer aroused. Serious threat to health of a woman resulted in prevention of pregnancy. Right side adnexectomy was conducted. Surgical treatment led to improvement and after four years the patient delivered a healthy child.

[Cytoreductive surgery and immunotherapy].

The aim of the article was to treat Pliss lymphosarcoma by means of Cytoreductive surgery to improve patient outcomes by taking into consideration the dynamics of Blood Auto-Proteolytic Activity (BAPA). In rats suffering from tumour proves to be obvious that higher the BAPA, more intensive is the process of invasive growth of tumour. Therefore, the performance of cytoreductive surgery is more reasonable when BAPA is at its minimal level, i. e. either at the early stage of tumour, though this may sound as a paradox. Removal of the tumour at the maximal level of BAPA will favour rapid development of a recurrent tumour. This can be explained by the fact that the remained cells tend to be more serious threat when BAPA level appears to be high.