RESUMO
A colorimetric probe TQA ((E)-4-(((8-(sec-butoxy)-2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-yl)methylene)amino)benzylacrylate) possessing greater potent towards the sensing of cysteine was successfully synthesized and characterized. The aqueous soluble probe TQA detects Cys based on "ON-OFF" effect with excellent absorbance and emission properties. The probe TQA detects Cys up to its ultra-low level concentration of 1.5 nM and also quantifies the Cys up to 5.05 nM with the quicker response time of 140 s (2.3 min). In addition, the color change produced by the probe TQA on integrated with Cys was also identified easily via paper strip, cotton wool buds and RGB color picker app in smart mobiles. Further, the admirable selectivity and sensitivity of the probe TQA towards Cys extends its utility towards food samples and imaging of live HeLa cells.
Assuntos
Cisteína , Água , Humanos , Células HeLa , Acrilatos , Corantes FluorescentesRESUMO
Two novel fluorescent probes, namely, 3-(2,4-dinitrophenoxy)-2-(4-(diphenylamino)phenyl)-4H-chromen-4-one (P1) and 3-(2,4-dinitrophenoxy)-2-(pyren-1-yl)-4H-chromen-4-one (P2), were designed and synthesized here. The probes (P1 and P2) were found to be highly selective and sensitive toward hydrogen sulfide (H2S) in the presence of a wide range of anions. The new probes (P1 and P2) were fully characterized by analytical, NMR spectroscopy (1H and 13C), and ESI mass spectrometry. The sensing capability of chemodosimeters (P1 and P2) toward H2S was confirmed by fluorescence studies. The 'turn-on' fluorescence was used to calculate the detection limit of probes (LOD), which were found to be 2.4 and 1.2 µM for P1 and P2, respectively. Moreover, the probes were tested for their cytotoxicity against HeLa cells using the MTT assay and found to be non-cytotoxic in nature; hence, the probes P1 and P2 were successfully utilized to visualize H2S in the living cells.