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J Int Med Res ; 33(6): 661-7, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-16372584

RESUMO

Local bone marrow (BM) renin-angiotensin system (RAS) affects physiological and pathological haematopoiesis, including erythropoiesis. In this study, quantitative expression of the messenger RNAs of the major RAS components--angiotensin-converting enzyme (CD143), renin and angiotensinogen--were measured in BM samples by quantitative real-time polymerase chain reaction, to evaluate the activity of local BM RAS in polycythemia rubra vera (PV) in comparison with normal erythropoiesis. The presence of CD143 was also investigated in the same BM samples by flow cytometry. Increased local synthesis of the major RAS components has been identified by demonstrating corresponding mRNAs in the BM of the patients with PV. Our findings indicate up-regulation of local BM RAS, together with down-regulation of the cell surface angiotensin-converting enzyme receptors, in the autonomous neoplastic clonal erythropoiesis of PV.


Assuntos
Células da Medula Óssea/metabolismo , Policitemia Vera/metabolismo , Sistema Renina-Angiotensina/genética , Adulto , Idoso , Angiotensinogênio/genética , Angiotensinogênio/metabolismo , Células da Medula Óssea/fisiologia , Estudos de Casos e Controles , Feminino , Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Peptidil Dipeptidase A/genética , Peptidil Dipeptidase A/metabolismo , Policitemia Vera/genética , Renina/genética , Renina/metabolismo
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