Assuntos
Haplótipos , Neoplasias Hematológicas/genética , Janus Quinase 2/genética , Modelos Genéticos , Mutação de Sentido Incorreto , Transtornos Mieloproliferativos/genética , Proteínas de Neoplasias/genética , Substituição de Aminoácidos , Feminino , Neoplasias Hematológicas/enzimologia , Humanos , Janus Quinase 2/metabolismo , Masculino , Transtornos Mieloproliferativos/enzimologiaRESUMO
Collagenolytic proteases from invertebrates, the active matter of new structure-destroying enzyme preparations (Collagenase from hydrobionts, Polycollagenase-K, Fermenkol), ensure deep hydrolysis of polypeptide substrates (native or partially denatured collagen types, elastin, fibrin, hemoglobin, and casein) that cannot be attained by collagenases and serine proteases. Biochemical properties of collagenolytic proteases from invertebrates, techniques and doses that may be important for elaboration and employment of preparations based on this complex are described.
Assuntos
Colágeno/metabolismo , Colagenases/metabolismo , Cosméticos , Invertebrados/metabolismo , Animais , Reprodutibilidade dos TestesRESUMO
In alimentary deficiency of vitamin K in rats, accompanied by an increase in the prothrombin time by 30%, activity of kidney creatine kinase and of blood serum alkaline phosphatase was unaltered, while the activity of alkaline phosphatase in small intestinal mucose was decreased by 20% and that of creatine kinase from skeletal muscles--by 10%. In vitamin K-deprived animals the rate of coupling between respiration and mitochondrial phosphorylation was decreased, which might be due to alteration in the NADH-dehydrogenase complex. Menadion reductase activity and cyanide-resistant respiration of mitochondria were unaltered in presence of menadion. Palmitic acid effectively activated of mitochondrial respiration in vitamin K-deprived animals (contrary to the control rats). This effect appears to occur as a result of structural alterations in mitochondria depending on vitamin K level in the organelles.
Assuntos
Metabolismo Energético , Deficiência de Vitamina K/enzimologia , Fosfatase Alcalina/metabolismo , Animais , Creatina Quinase/metabolismo , Mucosa Intestinal/enzimologia , Rim/enzimologia , Cinética , Mitocôndrias Hepáticas/metabolismo , Músculos/enzimologia , Consumo de Oxigênio/efeitos dos fármacos , Ácidos Palmíticos/farmacologia , Quinona Redutases/metabolismo , Ratos , Ratos Endogâmicos , Vitamina K/metabolismo , Deficiência de Vitamina K/metabolismoRESUMO
The influence of vitamin D and C deficiency on the kinetic parameters of sucrase and alkali phosphatase activities was studied in the microsomal fraction of the small intestinal mucosa of guinea pigs. It was found that Km values for these enzymes did not depend on the animal providing with these vitamins. Deficiency of one of these vitamins did not influence sucrase activity, however, simultaneous elimination of vitamins D and C resulted in the activity rise by 92%. Alkali phosphatase and Ca-ATPase activities proved to be similarly dependent on providing with vitamin D in the presence of vitamin C in the ration, while in the absence of vitamin C this dependence was not observed.