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Toxicol In Vitro ; 98: 105830, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38641231

RESUMO

Local drug delivery systems based on bioceramics ensure safe and effective treatment of bone defects and anticancer therapy. A promising drug delivery scaffold material for bone treatment applications is diopside (CaMgSi2O6) which is bioactive, degradable, and possesses drug-release ability. Currently, in vitro assessment of drug release from biomaterials is performed mostly on a 2D cell monolayer. However, to interpret and integrate biochemical signals, cells need a 3D microenvironment that provides cell-cell and cell-extracellular matrix interactions. In this regard, 3D cell models are gaining popularity. In this work, we proposed the protocol for evaluation of the effect of doxorubicin released from diopside on MG-63 cells and primary human fibroblasts in 3D culture conditions. Tissue spheroids with similar diameters were incubated with doxorubicin-loaded diopside for 72 h, the amount of diopside was calculated in accordance with the required doxorubicin concentration. We demonstrated that doxorubicin is gradually released from diopside and exhibits an activity similar to that of the pure drug at the same total concentration. It is important to note that doxorubicin was more potent on MG-63 spheroids compared to HF spheroids, which confirmed the reliability of spheroids as 3D models of tumor and healthy tissues.


Assuntos
Antibióticos Antineoplásicos , Doxorrubicina , Liberação Controlada de Fármacos , Esferoides Celulares , Humanos , Doxorrubicina/farmacologia , Esferoides Celulares/efeitos dos fármacos , Antibióticos Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Fibroblastos/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Técnicas de Cultura de Células em Três Dimensões/métodos
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