RESUMO
Eosinophils are differentiated in the bone marrow and transit through the blood circulation to home into tissues primarily under the regulation of IL-5. Because the number of eosinophils in the peripheral blood is relatively low under normal conditions, in vivo functional studies of eosinophils remain extremely difficult. Increasing their numbers in vivo might be useful for assessing eosinophil activities during parasite infections, allergic inflammation, and so on. Here, we provide a method for eosinophil expansion using IL-5 gene transfer by electroporation in vivo.
Assuntos
Técnicas de Cultura de Células/métodos , Eletroporação/métodos , Eosinófilos/citologia , Animais , Medula Óssea , Células da Medula Óssea/fisiologia , Diferenciação Celular , Eosinófilos/metabolismo , Técnicas de Transferência de Genes , Humanos , Inflamação , Interleucina-5 , Contagem de Leucócitos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BLAssuntos
Leishmania infantum/isolamento & purificação , Leishmaniose Visceral/diagnóstico , Adulto , Argentina , Medula Óssea/parasitologia , Citocromos b/genética , Humanos , Leishmania infantum/genética , Leishmaniose Visceral/sangue , Leishmaniose Visceral/parasitologia , Masculino , Tipagem Molecular , Parasitemia/sangue , Parasitemia/parasitologia , Proteínas de Protozoários/genética , Análise de Sequência de DNARESUMO
BACKGROUND: Information regarding leishmaniasis recidiva cutis (LRC), a clinical variant of cutaneous leishmaniasis, in the New World is scarce. LRC is characterized by slowly progressing lesion(s) that appear after a variable period of time, from months to years, in or around the scar of an apparently clinically healed sore. PATIENTS AND METHODS: Six patients are reported who presented with crusted, papular lesions located on the edge of a healed scar, with a mean of 18.2 months of slowly progressive evolution. The isolated strains of Leishmania parasites were characterized by enzyme electrophoresis. Eleven enzyme systems were assayed. Skin biopsies from the active border of the lesions were taken for histopathology. RESULTS: Tissue sections showed a granulomatous, lymphohistiocytic, dermal infiltrate containing Langhans' giant cells. The anamnestic data, together with the clinical and histopathologic findings, support the diagnosis of LRC. The isoenzyme profile of Leishmania parasites isolated from five of the six patients identified them as Leishmania (Viannia) panamensis. CONCLUSIONS: These findings are the first reported evidence of LRC within the clinical spectrum of American tegumentary leishmaniasis in Ecuador, and of its causative agent. The existence of LRC has future implications for both disease treatment and vaccine development.