Genetic dissection of grain iron and zinc, and thousand kernel weight in wheat (Triticum aestivum L.) using genome-wide association study.

Genetic biofortification is recognized as a cost-effective and sustainable strategy to reduce micronutrient malnutrition. Genomic regions governing grain iron concentration (GFeC), grain zinc concentration (GZnC), and thousand kernel weight (TKW) were investigated in a set of 280 diverse bread wheat genotypes. The genome-wide association (GWAS) panel was genotyped using 35 K Axiom Array and phenotyped in five environments. The GWAS analysis showed a total of 17 Bonferroni-corrected marker-trait associations (MTAs) in nine chromosomes representing all the three wheat subgenomes. The TKW showed the highest MTAs (7), followed by GZnC (5) and GFeC (5). Furthermore, 14 MTAs were identified with more than 10% phenotypic variation. One stable MTA i.e. AX-95025823 was identified for TKW in both E4 and E5 environments along with pooled data, which is located at 68.9 Mb on 6A chromosome. In silico analysis revealed that the SNPs were located on important putative candidate genes such as Multi antimicrobial extrusion protein, F-box domain, Late embryogenesis abundant protein, LEA-18, Leucine-rich repeat domain superfamily, and C3H4 type zinc finger protein, involved in iron translocation, iron and zinc homeostasis, and grain size modifications. The identified novel MTAs will be validated to estimate their effects in different genetic backgrounds for subsequent use in marker-assisted selection. The identified SNPs will be valuable in the rapid development of biofortified wheat varieties to ameliorate the malnutrition problems.

BODIPY-Hg2+ Complex: A Fluorescence "Turn-ON" Sensor for Cysteine Detection.

A BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) based pioneering sensing material (HLPy) having 2-amino pyridine as receptor was synthesized and used for the selective detection of Hg2+ ions. The synthesized HLPy features a high affinity towards Hg2+ (ka = 2.04 × 105 M-1), accompanied by effective quenching of fluorescence in DMF:H2O (1:9 v/v, 10 mM HEPES buffer, pH 7.4) with 54 nM limit of detection (LOD). The emission titration experiments (Job's plot) in the presence of varying mole-fraction of Hg2+ ions reveals the formation of non-fluorescent 2:1 coordination complex [Hg(LPy)2]. The resulting non-fluorescent [Hg(LPy)2] was thoroughly characterized using various spectroscopic techniques and analyses. Interestingly, the non-fluorescent complex [Hg(LPy)2] is able to specifically respond towards Cys over other biothiols and amino acids through a reversible de-complexation mechanism. As a result, the remarkable recovery of the fluorescence can be observed. The limit of detection (LOD) for Cys detection is estimated to be 29 nM in DMF:H2O (1:9 v/v, 10 mM HEPES buffer, pH 8.0). The reversibility and reusability of [Hg(LPy)2] were achieved by the sequential addition of Cys and Hg2+ ions up to five cycles. Moreover, the removal of Hg2+ ions up to 89% from aqueous samples using HLPy was successfully demonstrated.