Healing features of experimental injuries of soft tissues that contain foreign bodies in the form of fragments of military personnel uniforms.

INTRODUCTION: The healing of combat wounds can be complicated by the presence of foreign bodies. The present research aimed to study the regeneration of soft tissue injuries complicated by foreign bodies in the form of fragments of military uniforms. METHODS: 60 rats were divided into four groups: intact (Int), control (Con) and two experimental (EG1, EG2). In Con, wounds were sutured without implantation of foreign bodies. A uniform consisting of 100% cotton was used as foreign bodies for EG1 and a uniform consisting of 65% cotton and 35% polyester for EG2. Histological studies were performed according to generally accepted methods. The growth factors wеre determined by ELISA. RESULTS: In EG1, the inflammatory reaction proceeded protractedly, which complicated the development of granulation tissue. In EG2, considering the moderate inflammatory reactions, healing of the wounds became possible. The increase of the growth factors content in Con was maximal at the 15th day: basic fibroblast growth factor (bFGF)-2.2 times and vascular endothelial growth factor (VEGF)-1.6 times (p<0.001). In experimental groups, the bFGF content was maximal at the 60th day and exceeded the normal values by 1.7 times in EG1 and by 2.6 times in EG2 (p<0.001); the level of VEGF was slightly higher than that in healthy animals. CONCLUSIONS: The study showed wound healing in all groups. The repair of injuries in rats of EG1 and EG2 was complicated by the textile implant and took longer. The dynamics of the content of growth factors reflected the impairment of wound healing processes. These violations were more pronounced in the EG1 group.

INTERACTIONS OF RANKL, OSTEOPROTEGERIN AND ADIPOKINES IN REGULATION OF BONE REMODELING IN EXPERIMENTAL CHRONIC KIDNEY FUNCTION DISORDER.

RANKL and osteoprotegerin are the key cytokines of the axis regulating not only bone remodeling but also bone-kidney interrelations. They are connected with adipokines in the regulatory actions. The aim of the article was to investigate the role of the main parts of RANK-RANKL-osteoprotegerin axis and cytokines of additional link of regulatory network - adiponectin and visfatin and the interrelationship of these cytokines in chronic kidney disorder model in rats. Chronic kidney disorder model was created in the rats 12 weeks after intramuscular 50 % glycerol injection-induced acute kidney injury. RANKL, osteoprotegerin, adiponectin and visfatin levels was measured using ELISA. Increase of all cytokines and changes of their interrelations has been revealed in rats with chronic kidney disorder model. Conclusion was made that effects of the cytokine axis where RANKL is a trigger in the regulation network of interconnections «bone - kidney¼ has inflammatory character, so as visfatin effect, and adiponectin has opposite preserving bone effect.