RESUMO
The obligate intracellular parasite Toxoplasma gondii can actively infect any nucleated cell type, including cells from the immune system. The rapid transfer of T. gondii from infected dendritic cells to effector natural killer (NK) cells may contribute to the parasite's sequestration and shielding from immune recognition shortly after infection. However, subversion of NK cell functions, such as cytotoxicity or production of proinflammatory cytokines, such as gamma interferon (IFN-γ), upon parasite infection might also be beneficial to the parasite. In the present study, we investigated the effects of T. gondii infection on NK cells. In vitro, infected NK cells were found to be poor at killing target cells and had reduced levels of IFN-γ production. This could be attributed in part to the inability of infected cells to form conjugates with their target cells. However, even upon NK1.1 cross-linking of NK cells, the infected NK cells also exhibited poor degranulation and IFN-γ production. Similarly, NK cells infected in vivo were also poor at killing target cells and producing IFN-γ. Increased levels of transforming growth factor ß production, as well as increased levels of expression of SHP-1 in the cytosol of infected NK cells upon infection, were observed in infected NK cells. However, the phosphorylation of STAT4 was not altered in infected NK cells, suggesting that transcriptional regulation mediates the reduced IFN-γ production, which was confirmed by quantitative PCR. These data suggest that infection of NK cells by T. gondii impairs NK cell recognition of target cells and cytokine release, two mechanisms that independently could enhance T. gondii survival.
Assuntos
Imunomodulação , Células Matadoras Naturais/microbiologia , Células Matadoras Naturais/fisiologia , Toxoplasma/imunologia , Toxoplasmose Animal/imunologia , Animais , Citotoxicidade Imunológica , Células Dendríticas/imunologia , Células Dendríticas/microbiologia , Interações Hospedeiro-Parasita , Interferon gama/biossíntese , Interferon gama/imunologia , Células Matadoras Naturais/imunologia , Camundongos , Fosforilação , Proteína Tirosina Fosfatase não Receptora Tipo 6/biossíntese , Proteína Tirosina Fosfatase não Receptora Tipo 6/genética , Fator de Transcrição STAT4/metabolismo , Toxoplasma/fisiologia , Fator de Crescimento Transformador beta/biossínteseRESUMO
Recently, monocytic cells were suggested to systemically transport Toxoplasma tachyzoites during acute infection in mice. The mechanism underlying this shuttling function may partly be explained by dramatically enhanced host-cell motility upon parasite invasion. Here, we report that infection of human and murine macrophages in vitro resulted in augmented migration across a transwell membrane, linked to host-cell differentiation and to the parasite genotype. The hypermotility phenotype was absent in infected monocytes, NK, B or T-cells. In contrast to previous observations with dendritic cells, adoptive transfer of infected macrophages or lymphocytes did not exacerbate infection in mice compared to inoculation with free parasites.
Assuntos
Movimento Celular , Leucócitos/parasitologia , Toxoplasma , Toxoplasmose Animal/imunologia , Transferência Adotiva , Animais , Células Cultivadas , Células Dendríticas/parasitologia , Relação Dose-Resposta Imunológica , Humanos , Linfócitos/parasitologia , Macrófagos/parasitologia , Camundongos , Camundongos Endogâmicos C57BLRESUMO
Following intestinal invasion, the processes leading to systemic dissemination of the obligate intracellular protozoan Toxoplasma gondii remain poorly understood. Recently, tachyzoites representative of type I, II and III T. gondii populations were shown to differ with respect to their ability to transmigrate across cellular barriers. In this process of active parasite motility, type I strains exhibit a migratory capacity superior to those of the type II and type III strains. Data also suggest that tachyzoites rely on migrating dendritic cells (DC) as shuttling leukocytes to disseminate in tissue, e.g., the brain, where cysts develop. In this study, T. gondii tachyzoites sampled from the three populations were allowed to infect primary human blood DC, murine intestinal DC, or in vitro-derived DC and were compared for different phenotypic traits. All three archetypical lineages of T. gondii induced a hypermigratory phenotype in DC shortly after infection in vitro. Type II (and III) strains induced higher migratory frequency and intensity in DC than type I strains did. Additionally, adoptive transfer of infected DC favored the dissemination of type II and type III parasites over that of type I parasites in syngeneic mice. Type II parasites exhibited stronger intracellular association with both CD11c(+) DC and other leukocytes in vivo than did type I parasites. Altogether, these findings suggest that infected DC contribute to parasite propagation in a strain type-specific manner and that the parasite genotype (type II) most frequently associated with toxoplasmosis in humans efficiently exploits DC migration for parasite dissemination.
Assuntos
Movimento Celular , Células Dendríticas/imunologia , Células Dendríticas/parasitologia , Toxoplasma/genética , Toxoplasma/patogenicidade , Transferência Adotiva , Animais , Células da Medula Óssea/imunologia , Células Cultivadas , Células Dendríticas/fisiologia , Genótipo , Humanos , Camundongos , Especificidade da Espécie , Toxoplasma/crescimento & desenvolvimento , Toxoplasma/imunologiaRESUMO
The obligate intracellular parasite Toxoplasma gondii chronically infects up to one-third of the global population, can result in severe disease in immunocompromised individuals, and can be teratogenic. In this study, we demonstrate that death receptor ligation in T. gondii-infected cells leads to rapid egress of infectious parasites and lytic necrosis of the host cell, an active process mediated through the release of intracellular calcium as a consequence of caspase activation early in the apoptotic cascade. Upon acting on infected cells via death receptor- or perforin-dependent pathways, T cells induce rapid egress of infectious parasites able to infect surrounding cells, including the Ag-specific effector cells.
Assuntos
Citotoxicidade Imunológica , Perforina/metabolismo , Receptores de Morte Celular/metabolismo , Linfócitos T Citotóxicos/imunologia , Toxoplasma/imunologia , Animais , Apoptose , Cálcio/metabolismo , Caspases/metabolismo , Linhagem Celular , Humanos , Camundongos , Camundongos Congênicos , Perforina/genética , Linfócitos T Citotóxicos/parasitologia , Receptor fas/metabolismoRESUMO
The processes leading to systemic dissemination of the obligate intracellular parasite Toxoplasma gondii remain unelucidated. In vitro studies on human and murine dendritic cells (DC) revealed that active invasion of DC by Toxoplasma induces a state of hypermotility in DC, enabling transmigration of infected DC across endothelial cell monolayers in the absence of chemotactic stimuli. Infected DC exhibited upregulation of maturation markers and co-stimulatory molecules. While modulation of cell adhesion molecules CD11/CD18 was similar for Toxoplasma-infected DC and lipopolysaccharide (LPS)-matured DC, Toxoplasma-infected DC did not exhibit upregulation of CD54/ICAM-1. Induction of host cell migration in vitro required live intracellular parasite(s) and was inhibited by uncoupling the Gi-protein signalling pathway with pertussis toxin, but did not depend on CCR5, CCR7 or Toll/interleukin-1 receptor signalling. When migration of Toxoplasma-infected DC was compared with migration of LPS-stimulated DC in vivo, similar or higher numbers of Toxoplasma-infected DC reached the mesenteric lymph nodes and spleen respectively. Adoptive transfer of Toxoplasma-infected DC resulted in more rapid dissemination of parasites to distant organs and in exacerbation of infection compared with inoculation with free parasites. Altogether, these findings show that Toxoplasma is able to subvert the regulation of host cell motility and likely exploits the host's natural pathways of cellular migration for parasite dissemination.