'Do you remember the first time?' Host plant preference in a moth is modulated by experiences during larval feeding and adult mating.

In insects, like in other animals, experience-based modulation of preference, a form of phenotypic plasticity, is common in heterogeneous environments. However, the role of multiple fitness-relevant experiences on insect preference remains largely unexplored. For the multivoltine polyphagous moth Spodoptera littoralis we investigated effects of larval and adult experiences on subsequent reproductive behaviours. We demonstrate, for the first time in male and female insects, that mating experience on a plant modulates plant preference in subsequent reproductive behaviours, whereas exposure to the plant alone or plant together with sex pheromone does not affect this preference. When including larval feeding experiences, we found that both larval rearing and adult mating experiences modulate host plant preference. These findings represent the first evidence that host plant preferences in polyphagous insects are determined by a combination of innate preferences modulated by sensory feedback triggered by multiple rewarding experiences throughout their lifetime.

Insect host plant selection in complex environments.

Selection of suitable host plants is essential for the development and survival of herbivorous insects. Here we address behavioural mechanisms and the role of olfactory cues governing host choice, and their adaptive significance in complicated ecological contexts, with a focus on polyphagous insects. We also consider how recent developments in the study of olfactory systems of insects can provide a functional description of physiological mechanisms behind host plant choice. This may apply from the broader evolutionary history and local adaptations of olfactory receptor genes, to the underlying neural mechanisms behind innate host preferences and experience-based plasticity in host plant choice.

Comparison of plant preference hierarchies of male and female moths and the impact of larval rearing hosts.

Selection of a suitable host plant is essential for the fitness of herbivorous insects. For polyphagous insects the underlying proximate mechanisms for host plant selection, including phenotypic plasticity, remain only partially understood. We established an experimental protocol evaluating preferences to five plant species in males and females of the polyphagous moth Spodoptera littoralis. Female preference hierarchies were assessed by oviposition decisions; those of males were assessed by the attraction to female sex pheromones in background odors of different plant species. The experiments revealed clear preference hierarchies in both males and females, which were partly overlapping in spite of the different behavioral contexts of the respective assays. Furthermore, we demonstrated strong effects of the larval rearing host on adult plant preference, where the larval host plant species was generally elevated to the most preferred plant in both sexes, without otherwise affecting the overall preference hierarchy. Our results suggest that both sexes are involved in host plant choice and that experience-based convergent intersexual plant preferences may confer selective advantages. The host plant choice is guided by a stable plant preference hierarchy, which can be modified by the larval rearing host, permitting fast adaptation to variation in local conditions and to novel environments. It may also provide a mechanism for reducing costs associated with polyphagy by functional plasticity in plant choice.

Conserved, highly specialized olfactory receptor neurons for food compounds in 2 congeneric scarab beetles, Pachnoda interrupta and Pachnoda marginata.

Few studies have systematically addressed evolutionary changes in olfactory neuron assemblies, either by genetic drift or as an adaptation to specific odor environments. We have studied the sense of olfaction in 2 congeneric scarab beetles, Pachnoda interrupta Olivier and Pachnoda marginata Drury (Coleoptera: Scarabaeidae: Cetoniinae), which are both opportunistic polyphages, feeding mainly on fruit and flowers. The 2 species occur in dissimilar habitats: P. interrupta is found in dry savannah, and P. marginata in tropical parts of equatorial Africa. To study how these species may have adapted their sense of olfaction to their odor environments, we utilized single-unit electrophysiology on olfactory sensilla with a wide selection of food-related compounds. Despite the differences in habitat, we found that the species shared most of the physiological types of olfactory receptor neurons (ORNs) encountered, although their proportions frequently varied between the species. The high degree of conservation in olfaction between the species implies that a similar sensory strategy is efficient for food search in both habitats. However, shifts in proportions of receptor neuron classes, and slight shifts in response profiles and/or presence of some ORN classes unique to either species, may reflect adaptation to a different set of hosts.