The Effect of Curcuma phaeocaulis Valeton (Zingiberaceae) Extract on Prion Propagation in Cell-Based and Animal Models.

Prion diseases are neurodegenerative disorders in humans and animals for which no therapies are currently available. Here, we report that Curcuma phaeocaulis Valeton (Zingiberaceae) (CpV) extract was partly effective in decreasing prion aggregation and propagation in both in vitro and in vivo models. CpV extract inhibited self-aggregation of recombinant prion protein (PrP) in a test tube assay and decreased the accumulation of scrapie PrP (PrPSc) in ScN2a cells, a cultured neuroblastoma cell line with chronic prion infection, in a concentration-dependent manner. CpV extract also modified the course of the disease in mice inoculated with mouse-adapted scrapie prions, completely preventing the onset of prion disease in three of eight mice. Biochemical and neuropathological analyses revealed a statistically significant reduction in PrPSc accumulation, spongiosis, astrogliosis, and microglia activation in the brains of mice that avoided disease onset. Furthermore, PrPSc accumulation in the spleen of mice was also reduced. CpV extract precluded prion infection in cultured cells as demonstrated by the modified standard scrapie cell assay. This study suggests that CpV extract could contribute to investigating the modulation of prion propagation.

Influence of interfering co-appearing container peaks on the accuracy of direct quantitative Raman measurement of a sample in a plastic container.

The axially perpendicular offset (APO) scheme was previously demonstrated as a versatile scheme able to minimize or eliminate the glass background in the direct and non-sampling Raman measurement of an ethanol sample housed in a glass bottle. Alternatively, when directly analyzing a sample housed in a plastic container, another typical container yielding strong Raman peaks itself, the Raman peaks of both the container and the housed sample are unavoidably present together in a collected spectrum. Therefore, a crucial issue to investigate under this situation is how the magnitude of the co-appearing container peaks influences the accuracy for quantitative analysis of the housed sample. For the evaluation, a non-sampling Raman spectroscopic measurement of the urea concentration in a urea gel housed in a circular polypropylene (PP) container was attempted by employing two axially perpendicular offset (APO) schemes with detection windows of different sizes (25.4 and 10.0 mm, referred to as the wide-window APO (WW-APO) and narrow-window APO (NW-APO), respectively), and transmission and back-scattering schemes incorporating a 25.4 mm detection window. The intensity ratios between the container and urea peaks in the collected spectra were different depending on the adopted measurement scheme. The intensity ratio was greatest (smallest container peak) in the NW-APO measurement due to the narrowed detection window, making the generated container Raman photons at the side-wall less detectable to the bottom-positioned detector. A spectral acquisition scheme allowing the maximal suppression of the container peaks, while still maintaining the sample features, was a key requirement to secure an accurate measurement of the sample concentration. In addition, a Monte Carlo simulation was used to visualize the distributions of the container and urea photons inside the sample-housed container.

Free and bound form bioactive compound profiles in germinated black soybean (Glycine max L.).

This study investigated the transition between the free and bound forms of functional compounds in germinated black soybean. Black soybean was germinated at 25°C over 6 days and then the free and bound forms of functional compounds were extracted. Total free polyphenol, flavonoid, and phenolic acid contents in raw black soybean increased from 1.03 mg GAE/g, 0.29 mg CE/g, and 315.67 µg/g to 1.44mg GAE/g, 0.64mg CE/g, and 511.01 µg/g, respectively, by 4 days after germination. Changes to phenolic acid compositions can be divided into four groups, and the germination process can convert compounds to phenolic acid via anabolism and catabolism. The highest total free isoflavone content in germinated black soybean (3,724.40 µg/g) was observed at 4 days. Bound polyphenol, flavonoid, phenolic acid, and isoflavone contents decreased as the germination period increased. These results suggest that the germination process increased compound functionality in black soybean.