Exosomes from umbilical cord mesenchymal stromal cells promote the collagen production of fibroblasts from pelvic organ prolapse.

BACKGROUND: Pelvic organ prolapse (POP) involves pelvic organ herniation into the vagina due to pelvic floor tissue laxity, and vaginal structure is an essential factor. In POP, the vaginal walls exhibit abnormal collagen distribution and decreased fibroblast levels and functions. The intricate etiology of POP and the prohibition of transvaginal meshes in pelvic reconstruction surgery present challenges in targeted therapy development. Human umbilical cord mesenchymal stromal cells (hucMSCs) present limitations, but their exosomes (hucMSC-Exo) are promising therapeutic tools for promoting fibroblast proliferation and extracellular matrix remodeling. AIM: To investigate the effects of hucMSC-Exo on the functions of primary vaginal fibroblasts and to elucidate the underlying mechanism involved. METHODS: Human vaginal wall collagen content was assessed by Masson's trichrome and Sirius blue staining. Gene expression differences in fibroblasts from patients with and without POP were assessed via RNA sequencing (RNA-seq). The effects of hucMSC-Exo on fibroblasts were determined via functional experiments in vitro. RNA-seq data from fibroblasts exposed to hucMSC-Exo and microRNA (miRNA) sequencing data from hucMSC-Exo were jointly analyzed to identify effective molecules. RESULTS: In POP, the vaginal wall exhibited abnormal collagen distribution and reduced fibroblast 1 quality and quantity. Treatment with 4 or 6 µg/mL hucMSC-Exo suppressed inflammation in POP group fibroblasts, stimulated primary fibroblast growth, and elevated collagen I (Col1) production in vitro. High-throughput RNA-seq of fibroblasts treated with hucMSC-Exo and miRNA sequencing of hucMSC-Exo revealed that abundant exosomal miRNAs downregulated matrix metalloproteinase 11 (MMP11) expression. CONCLUSION: HucMSC-Exo normalized the growth and function of primary fibroblasts from patients with POP by promoting cell growth and Col1 expression in vitro. Abundant miRNAs in hucMSC-Exo targeted and downregulated MMP11 expression. HucMSC-Exo-based therapy may be ideal for safely and effectively treating POP.

Contrasting impacts of fertilization on topsoil and subsoil greenhouse gas fluxes in a thinned Chinese fir plantation.

Globally, forest soils are considered as important sources and sinks of greenhouse gases (GHGs). However, most studies on forest soil GHG fluxes are confined to the topsoils (above 20 cm soil depths), with only very limited information being available regarding these fluxes in the subsoils (below 20 cm soil depths), especially in managed forests. This limits deeper understanding of the relative contributions of different soil depths to GHG fluxes and global warming potential (GWP). Here, we used a concentration gradient-based method to comprehensively investigate the effects of thinning intensity (15% vs. 35%) and nutrient addition (no fertilizer vs. NPK fertilizers) on soil GHG fluxes from the 0-40 cm soil layers at 10 cm depth intervals in a Chinese fir (Cunninghamia lanceolata) plantation. Results showed that forest soils were the sources of CO2 and N2O, but the sinks of CH4. Soil GHG fluxes decreased with increasing soil depth, with the 0-20 cm soil layers identified as the dominant producers of CO2 and N2O and consumers of CH4. Thinning intensity did not significantly affect soil GHG fluxes. However, fertilization significantly increased CO2 and N2O emissions and CH4 uptake at 0-20 cm soil layers, but decreased them at 20-40 cm soil layers. This is because fertilization alleviated microbial N limitation and decreased water filled pore space (WFPS) in topsoils, while it increased WFPS in subsoils, ultimately suggesting that soil WFPS and N availability (especially NH4+-N) were the predominant regulators of GHG fluxes along soil profiles. Generally, there were positive interactive effects of thinning and fertilization on soil GHG fluxes. Moreover, the 35% thinning intensity without fertilization had the lowest GWP among all treatments. Overall, our results suggest that fertilization may not only cause depth-dependent effects on GHG fluxes within soil profiles, but also impede efforts to mitigate climate change by promoting GHG emissions in managed forest plantations.

Rhizosphere environmental factors regulated the cadmium adsorption by vermicompost: Influence of pH and low-molecular-weight organic acids.

Vermicompost is a promising amendment for immobilization of cadmium (Cd) in soils; however, its effectiveness can be influenced by rhizosphere environment conditions, such as pH and the presence of low-molecular-weight organic acids (LMWOAs). In this study, a batch experiment was conducted to examine the characteristics of Cd adsorption by vermicompost at different pH (pH = 3, 5, and 7) and after the addition of different LMWOAs (oxalic acid; citric acid; malic acid). Furthermore, a series of morphology and structural analyses were conducted to elucidate the mechanisms of observed effects. The results showed that the adsorption capacity of vermicompost for Cd increased as pH increased, and chemisorption dominated the adsorption process. Changes in pH altered adsorption performance by affecting the -OH groups of alcohol/phenol and the -CH2 groups of aliphatics. Further, the addition of oxalic acid promoted Cd adsorption, and the effect was concentration dependent. Modifying the verimicompost surface with more adsorption sites might be the main reason. Conversely, citric acid and malic acid showed the ability to inhibit Cd adsorption by vermicompost. Citric acid caused a blocking effect by covering flocculent substances on the vermicompost surface while reducing surface adsorption sites by dissolving mineral components such as iron oxides. However, the action of malic acid did not appear to be related to changes in morphology or the structure of vermicompost. Overall, the results of this study partially explain the limited effectiveness of Cd immobilization within the rhizosphere by vermicompost, and provide theoretical support for regulating rhizosphere environments to improve the effectiveness of vermicompost immobilization of Cd.

Clinical Characteristics of Tracheobronchopathia Osteochondroplastica: A Retrospective Study of 33 Patients.

Introduction: Tracheobronchopathia osteochondroplastica (TO) is a relatively rare benign tracheobronchial disease, which is often misdiagnosed or missed. To date, there is no specific treatment for TO. The aim of this study was to investigate the clinical manifestations, imaging features, bronchoscopy results, pathological findings, and diagnostic points of TO. Patients and methods: A total of 33 patients diagnosed with TO were enrolled. Clinical data were collected using retrospective methods in the period from January 2021 and November 2022. Descriptive analysis was used. Results: Patients included 17 (51.5%) male and 16 (48.5%) female, with a median age of 54 years. The main clinical manifestations included cough in 15 cases, fever in 6 cases, chest tightness in 4 cases, haemoptysis in 3 cases, and chest pain in 4 cases. The time from the onset of symptoms to diagnosis was 1 week to 96 months. Some patients were diagnosed with other lung diseases, including 16 patients with tuberculosis, 2 patients with lung cancer, 3 patients with nontuberculous mycobacteriosis, 3 patients with tuberculous pleurisy, 2 patients with bronchiectasis, and 1 patient with pneumonia. Chest computed tomography (CT) scan demonstrated calcified nodules in 10 (30.3%) patients. In bronchoscopy, entire tracheal involvement was found in 21 (63.6%) patients, 12 (36.4%) patients were found to have involvement of only part of the trachea. The patients were divided into three groups according to the bronchoscopic presentation, the largest proportion was stage II (19/33), followed by stage I (8/33) and stage III (6/33). Histopathological findings showed squamous metaplasia, cartilaginous, and bony tissues. Conclusion: TO is a slowly progressing disease with non-specific clinical symptoms and a low positive rate of imaging diagnosis, making it susceptible to misdiagnosis and missed diagnosis. The disease needs to be diagnosed by combining imaging features, fiberoptic bronchoscopy, and pathological findings.

[Species, Habitat Characteristics, and Screening Suggestions of Cadmium Hyperaccumulators in China].

Phytoremediation, as a green and effective in-situ remediation technology for heavy metal-contaminated soil, has attracted the attention of Chinese scholars and has resulted in a series of achievements over the past 20 years. In this study, the species characteristics, distribution of field discovery sites in various vegetation zones, habitat characteristics, habitat geological characteristics, and geochemistry of cadmium (Cd) of the Cd hyperaccumulators in China reported in the relevant literature from the past 20 years (from 2002 to 2021) were summarized by searching for related keywords. Finally, suggestions were proposed for the screening of new Cd hyperaccumulators. The results showed that a total of 45 species of Cd hyperaccumulators in China have been reported so far. In terms of plant species, they belonged to 22 families and 36 genera, among which Compositae with 14 species was the most abundant. There were 25 species discovered through the field investigation, which were mainly distributed in the subtropical broadleaf evergreen forest region of southern China. Additionally, the Cd hyperaccumulators discovered by field surveys were mainly found in high Cd-concentrated soils surrounding lead-zinc mines. In conclusion, abundant plant resources, high concentrations of heavy metal soils, and long-term domestication jointly promoted the formation of hyperaccumulators. Therefore, the region with these three points could be considered a high probability region for the presence of hyperaccumulators, and the screening of hyperaccumulators could be carried out around this. We proposed that the screening of new hyperaccumulators can be carried out through the following six steps:the identification and investigation of high probability areas, the enrichment capability test, the enrichment capability test in low concentration levels, the enrichment capability test between different ecotypes, the succession of enrichment capability, and the test of remediation proficiency.

Spatiotemporal distribution characteristics of potentially toxic elements in agricultural soils across China and associated health risks and driving mechanism.

Comprehensive analysis of spatiotemporal distribution characteristics of potentially toxic elements (PTEs) in soils of China, and associated driving mechanism and health risks is crucial for soil pollution prevention and risk control. In this study, a total of 8 PTEs in agricultural soils of 236 city case data from 31 provinces of China were collected from literatures published between 2000 and 2022. The pollution level, dominant drivers and probabilistic health risks of PTEs were analyzed using geo-accumulation index (Igeo), geo-detector model, and Monte Carlo simulation, respectively. The results indicated a notably accumulation of Cd and Hg, with Igeo of 1.13 and 0.63, respectively. Cd, Hg and Pb showed strong spatial heterogeneity, whereas As, Cr, Cu, Ni, and Zn has no significant differentiation. PM10 was the dominant driver for accumulation of Cd (0.248), Cu (0.141), Pb (0.108), and Zn (0.232), and PM2.5 has a significant effect on accumulation of Hg (0.245); however, soil parent material was primary driver for accumulation of As (0.066), Cr (0.113), and Ni (0.149). PM10 ∩ wind speed accounted for 72.6 % of Cd accumulation, and mining industry ∩ soil parent materials for 54.7 % of As accumulation. Approximately 38.53 %, 23.90 %, and 12.08 % of the hazard index values exceeded 1 for the minors aged 3- < 6, 6- < 12, and 12- < 18 years, respectively; whereas approximately 0.22 %, 0.21 %, 0.20 %, 0.54 % and 0.42 % of the carcinogenic risk values were greater than 1E-04 for individuals aged 3- < 6, 6- < 12, 12- < 18, 18- < 44, and 44- < 70 years, respectively. As and Cd were considered as priority elements for soil pollution prevention and risk control in China. Moreover, the hotspots of PTE pollution and associated health risks were mainly observed in southern, southwestern and central China. The results of this study provided a scientific basis for developing strategies for pollution prevention and risk control of soil PTEs in China.

Challenges and opportunities for improving the environmental quality of cadmium-contaminated soil in China.

Considering the soil cadmium pollution problem, the Chinese government proposed to estimate the costs and practicality "to completely improve the soil quality by the middle of this century". This study analyzed the challenges in achieving this goal using biophysical data from 10 typical demonstration soil phytoextraction projects. The current annual phytoextraction efficiency was determined as 14.8-490 g ha-1 a-1 at 319 RMB g-1 cadmium. A total of 798 billion RMB and 5 years were required for remediation of cadmium contamination, which was 22 times the investment in soil remediation during 2016-2022. The break-even point of phytoextraction projects was 29 years. The heavy financial burden was considered the primary challenge in improving the environmental quality of such soil. The cost could be reduced by 5.5-35.3 % through optimization measures such as resourcefulness of hyperaccumulator harvests, large-scale breeding, and mechanized management. The break-even point could be shortened to 6-15 years by intercropping/rotating crops, contributing to the goal. Active exploration of phytoextraction efficiency-more efficient accumulators, optimized agronomic measures-is worth practicing.

Human health risk apportionment from potential sources of heavy metals in agricultural soils and associated uncertainty analysis.

Evaluating heavy metal pollution level in the soils and apportioning the source-specific health risk of heavy metals are critical for proposing environmental protection and remediation strategies to protection human health. This study explored heavy metal pollution and associated source-specific health risks in a typical rural industrial area, southwestern China. A total of 105 topsoil samples were collected and the concentrations of heavy metals, including As, Cd, Cr, Cu, Ni, Pb and Zn, were determined. Pollution load index and enrichment factors were used to evaluate the pollution level of heavy metals. Positive matrix factorization (PMF) model was applied to apportion the heavy metals and the associated source-specific health risks to adults and children were estimated via combining the PMF model with the health risk assessment. The results indicated that the soils were highly polluted by multiple heavy metals, especially for Cd, with the EF values of 24.94 and 22.55 in the upstream and downstream areas, respectively. Source apportionment results showed that atmospheric deposition, smelting activities, fertilizer and sewage application, and agrochemical utilization were the main anthropogenic sources, with the contributions of 37.11%, 23.69%, 19.69%, and 19.51%, respectively. Source-specific risk assessment identified atmospheric deposition as the priority source for the non-carcinogenic (NCR) and carcinogenic risks (CR) in the study area, with the contribution of 43.71% and 52.52% for adults, and 44.29% and 52.58% for children, respectively. Moreover, non-carcinogenic and carcinogenic risks posed to children (NCR: 2.84; CR: 1.31 × 10-4) from four sources was higher than those posed to adults (NCR: 0.29; CR: 5.86 × 10-5). The results of source-specific health risk assessment provided the valuable information on the priority sources for pollution preventing and risk controlling.

Changes in arsenic accumulation and metabolic capacity after environmental management measures in mining area.

Due to the public health concern of arsenic, environmental management measures in mining areas had been implemented. To assess the effect of environmental management measures in the mining area comprehensively, arsenic accumulation in the urine, hair, nails, and urinary metabolites of residents in a realgar mining area in Hunan province, China were investigated in 2019, and the changes in arsenic levels in the biomarkers during 2012-2019 were tracked. The importance of confounding factors (age, sex, occupation, residence, clinical history, vegetable source, cooking fuel, smoking, alcohol consumption, BMI) was analyzed using the Boruta algorithm. After the implementation of environmental management measures (including ceasing mining and smelting activities, building landfills, adjusting the planting structure, and soil restoration), urine, hair, and nail arsenic concentration decreased drastically but were still excessive. Arsenic accumulation was highest in older male miners who were long settled in the mining area and consumed homegrown vegetables. The only factor for changes in urinary arsenic levels was the cooking fuel type; residents using wood as cooking fuel experienced sustained arsenic exposure. Occupation and sex were important for determining arsenic changes in the hair and nails. Short-term arsenic accumulation in urine was affected by arsenic exposure, while long-term accumulation in hair and nails by arsenic metabolic capacity. The percentage of urinary arsenic metabolism and arsenic methylation indices of the participants in the mining area were within the normal range (%iAs: 10-30 %, %MMA: 10-20 %, % DMA: 60-80 %); samples indicated worse metabolic capacity than the reference population. The arsenic metabolic capacity of male miners was relatively weak, probably aggravated by alcohol drinking and smoking. Without soil remediation, arsenic exposure will continue. Homegrown vegetables and biomass fuels should be abandoned; reduced cigarette and alcohol consumption is recommended. Urinary arsenic would be more proper for assessing environmental remediation in mining areas.

An interpolation method incorporating the pollution diffusion characteristics for soil heavy metals - taking a coke plant as an example.

The existing spatial interpolation methods in the prediction of soil heavy metal distribution are generally based on spatial auto correlation theory, rarely considering the pollution patterns. By contrast, in polluted sites, heavy metals have a strong heterogeneity even within a very small area, which is not exactly in line with auto correlation theory. This contradiction may lead to inaccuracy in spatial prediction. Atmospheric diffusion and deposition are one of the main sources of soil heavy metal pollution caused by coal-related production activities. To improve the prediction accuracy, the diffusion patterns of pollutants were considered in this paper by integrating Geodetector, Co-Kriging (COK), and partition interpolation. Geodetector was used to identify the main driving factors of soil pollution, based on which, the main driving factors were used as covariates introduced into the interpolation method (COK). Specifically, the amount of particulate matter deposition obtained by a pollutant diffusion model (AERMOD) was used as a covariate. For comparison, the distances to quenching, coke oven, and ammonium sulfate section were also used as covariates. Compared with the Ordinary Kriging method, the method COK-AERMOD established here decreased the root mean square error values of As (2.05 reduced to 1.89), Cd (0.18 reduced to 0.16), Cr (19.07 reduced to 12.97), Cu (6.92 reduced to 4.72), Hg (0.32 reduced to 0.28), Ni (16.92 reduced to 16.10), Pb (18.29 reduced to 16.62), and Zn (159.68 reduced to 153.66). This method in this paper is informative for the interpolation of soil elements in contaminated areas with known pollution source and diffusion patterns.

Knowledge Mapping of the Phytoremediation of Cadmium-Contaminated Soil: A Bibliometric Analysis from 1994 to 2021.

Cadmium pollution of soil threatens the environmental quality and human health. Phytoremediation of cadmium-contaminated soil has attracted global attention in recent decades. This study aimed to conduct a comprehensive and systematic review of the literature on phytoremediation of cadmium-contaminated soil based on bibliometric analysis. A total of 5494 articles published between 1994 and 2021 were retrieved from the Web of Science Core Collection. Our knowledge mapping presented the authors, journals, countries, institutions, and other basic information to understand the development status of phytoremediation of cadmium-contaminated soil. Based on a keyword cluster analysis, the identified major research domains were "biochar", "Thlaspi caerulescens", "endophytic bacteria", "oxidative stress", "EDTA", and "bioconcentration factor". Overall, this study provided a detailed summary of research trends and hotspots. Based on the keyword co-occurrence and burst analysis, the core concepts and basic theories of this field were completed in 2011. However, the pace of theoretical development has been relatively slow. Finally, future research trends/frontiers were proposed, such as biochar addition, rhizosphere bacterial community manipulation, cadmium subcellular distribution, and health risk assessment.

Accumulation, environmental risk characteristics and associated driving mechanisms of potential toxicity elements in roadside soils across China.

Roadside soils may be affected by potential toxicity elements (TPEs) from vehicles; however, pollution status, ecological and health risks of PTEs in roadside soils were rarely reported on national scale. In this study, a dataset of PTEs in roadside soils was compiled based on the literatures published in 2000-2021, and then pollution level, ecological and health risks of PTEs were evaluated using geochemical accumulation index (Igeo), potential ecological risk index (ER), and human risk assessment coupled with Monte Carlo simulation. Driving factors of PTE accumulation in soils were determined by Geo-detector method. Results indicated that Cd exhibited moderate pollution and considerable ecological risk with the highest Igeo of 1.25 and ER of 100.1, respectively. Vehicle ownership (VP) and precipitation (PP) significantly affected accumulation of PTEs, with q values of 0.209 and 0.191 (P < 0.05), respectively. VP paired with PP enhanced nonlinearly PTE accumulation (q = 0.77). Only 6.89% and 1.54% of non-carcinogenic risks for children and adolescent exceeded the threshold of 1, respectively, whereas 93.11%, 95.67%, 58.80% and 58.14% of carcinogenic risks for senior, adults, adolescent, and children surpassed 1E-06, respectively. The results of this study provided valuable implication for managers to design effective strategies for pollution prevent and risk control.

Source-specific health risks apportionment of soil potential toxicity elements combining multiple receptor models with Monte Carlo simulation.

Understanding the source-specific human health risk of soil potential toxicity elements (PTEs) for human is beneficial for pollution control and risk prevention. Multivariate statistics, absolute principal component score/multiple linear regression (APCS/MLR) model, positive matrix factorization (PMF) model, and GIS mapping were used to identify and apportion the sources of soil PTEs in typical mining and industrial area, southwestern China. Furthermore, source-specific health risks were apportioned by combining source apportionment with probabilistic health risk assessment based on Monte Carlo simulation which can define the probability that the risk exceed the guideline threshold value. The pollution factor and geo-accumulation index indicated that the soils were polluted by soil PTEs to different degrees. In particular, As and Cd were the primary pollutants. Mixed sources, agricultural activities, mining activities, and As-related smelting activities represented the potential sources of soil PTEs, with the contribution of 30.13%, 25.78%, 22.93%, and 21.16%, respectively. Source-specific probabilistic health risks indicated that As-related smelting activities contributed the most to non-carcinogenic risks (adults: 59.03%, children: 57.20%) and carcinogenic risks (adults: 81.82%; children 92.33%), despite the observation that it contributed the least to the accumulation of soil PTEs (21.16%). Non-carcinogenic and carcinogenic risk showed similar trend for children and adults. Therefore, As-related smelting activities were regarded as the priority source of soil PTEs, and corresponding prevention and control strategies should be implemented to protect human health.

Potential, risks, and benefits of the extract recycled from Pteris vittata arsenic-rich biomass as a broiler growth promoter.

The arsenic-rich biomass of Pteris vittata is a heavy burden to phytoremediation, but the compositions of extracts recycled from arsenic-rich biomass, such as rutin, may promote broiler growth. As such, this extract can be used to reduce the usage of antibiotics in the broiler industry and the cost of phytoremediation at the same time. Therefore, the critical issues for using extract from arsenic-rich biomass as a growth promoter have been studied, including its effective composition, health and environmental risks, and potential benefits and feasibility. Forty-five compounds were identified in the extract, and they were mainly flavonoids, chlorogenic acids, and proanthocyanidins, which can directly or indirectly influence the growth of broiler. The lifetime carcinogenic risks of broiler edible parts may be maximally increased by 4.75 × 10-9 due to feeding the extract. The arsenic concentration of the farmland fertilized with the excrement from the broiler fed with the extract may increase by 0.00003-0.01857 mg/kg per year. Results revealed a feasible scenario that the sustainability of phytoremediation and broiler industry could be benefited through wastes from each other.

Method on site-specific source apportionment of domestic soil pollution across China through public data mining: A case study on cadmium from non-ferrous industries.

The lack of emission data of major Cd-emitting enterprises has long limited the source apportionment of soil cadmium (Cd). Non-ferrous metal enterprises (NMEs) contribute the most Cd emissions in China in recent years. We estimated the cumulative Cd emission of 8750 NMEs across China through public data collection and material balance methods for the first time. The results showed that the total Cd emissions were estimated at 133,177 tons, of which 78.68% contributed by zinc primary smelting and mining. The emission hotspots are mainly concentrated in the south of the Yangtze River, such as Nanling Mountain areas, Nanpan River Basin, and Jincheng River Basin, as well as a few parts of the North and Northwest China. Then a significant positive spatial correlation was furtherly detected between NMEs and soil Cd, except for secondary smelting enterprises. Moreover, the hotspots of soil Cd pollution caused by NMEs were identified across China. By promoting the accounting calibrator from annual emission intensity of regional (mainly provincial) scale to the cumulative emission of site-specific enterprise in its entire life cycle, this study realized the finer description of the spatial heterogeneity of Cd emission from non-ferrous industry on a large scale and make it possible to refine the reliability of follow-up site-specific source apportionment, by introducing the emission intensity instead of the enterprise sites density. Finally, a modified approach for the regional source apportionment of soil pollution was proposed to obtain a more realistic and precise drawing. The results pointed out key NMEs subcategories and the affected hotspots which require continuous strengthening of Cd-related rectification. This methodological framework is expected to contribute to the precise management and differential sources control of Cd pollution and can be further extended to other pollutants for the precise targeting of key industries and hotspots during source pollution control in the future.

Source-specific ecological and health risks of potentially toxic elements in agricultural soils in Southern Yunnan Province and associated uncertainty analysis.

Source-specific risk apportionment is critical to prevent and control soil potentially toxic element (PTE) pollution. This study explored source-specific ecological and human health risks of soil PTEs in Southern Yunnan Province. Geochemical baseline values were determined to assess the pollution level of PTEs; then source-specific risk was apportioned combining positive matrix factorization (PMF) with ecological and human health risk assessment. Obvious accumulation of As, Cd, Pb, and Zn was observed in this area, especially Cd in 21.33% of the samples exhibited significant enrichment. Four sources were quantified based on PMF assisted with GIS-mapping: natural sources (41.49%), traffic emissions (24.70%), industrial activities (17.48%), and agricultural activities (16.33%). Industrial activities were the largest source (64.55%) to ecological risk. Agricultural activities were regarded as the major contributor to non-carcinogenic (adults: 75.93%, children: 62.33%) and carcinogenic risks (adults: 55.97%, children: 56.36%). Non-carcinogenic and carcinogenic risks for children were higher than adults, and their health risks showed similar trend. Thus, agricultural activities should be regarded as a priority to reduce health risk, whereas industrial activities should be given priority to control ecological risk. Although source-specific risk was quantified, combination with bioavailability and interactions of PTEs are necessary to obtain more accurate results in future.

Effective strategy to recycle arsenic-accumulated biomass of Pteris vittata with high benefits.

Recycling the arsenic-rich biomass of Pteris vittata is a critical problem during phytoremediation primarily because of the low value and high risk of arsenic-rich biomass. Nevertheless, extracts of P. vittata have been found to have a variety of bio-activities (e.g., anti-oxidation, anti-cancer, and anti-bacterial) and abundant valuable bio-active compositions (e.g., flavonoids), which might present a new solution for the recycling of P. vittata harvests. This work demonstrated a pilot-scale experiment to extract and purify the phenolic compounds from 1 t of arsenic-rich P. vittata biomass. Result showed that 47.9 kg of phenolic-rich extract with a potential value of US$908.66-8345.14 was obtained. This extract showed no acute oral toxicities (LD50 > 10 g/kg), no skin irritation, and no chronic risks in the long-term skin contact exposure pathways. All of the wastes from production have been recycled and safely disposed with low cost (US$28.44), and the cost may be further reduced. The calculated benefits from this method showed a potential to provide 995-53,050 US$/hm2 per year to a phytoremediation project. Therefore, this strategy could address the issue of expensive phytoremediation.

Dexamethasone prevents the Epstein-Barr virus induced epithelial-mesenchymal transition in A549 cells.

Clinical data have shown that pulmonary interstitial fibrosis is likely to occur in the later stages of viral pneumonia. While viral infections are thought to cause chronic pulmonary interstitial inflammation and pulmonary fibrosis, it remains unclear if they promote pulmonary fibrosis by epithelial-mesenchymal transition (EMT). In this study, human epithelial cell line A549 has been used to model the infection of the Epstein-Barr virus (EBV) and the respiratory syncytial virus (RSV). Their differences were compared and the possible infection mechanisms analyzed by randomly assigning cells to one of five treatments. Exposure of the LMP1 is thought to be the key gene during EBV-induced EMT in the A549 cells. Enzyme-linked immunosorbent assay analysis revealed that the EBV infection was associated with the induction of a number of cytokines (interleukin-8 [IL-8], IL-13, tumor necrosis factor-α, and transforming growth factor-ß) and dexamethasone (DXM) could significantly prevent the phenotypic changes, and partly the mechanisms related with the IL-13 pathway. Surprisingly, different results were seen with the RSV infection as the A549 cells still displayed an epithelial morphology but the levels of E-cadherin, α-SMA, vimentin, and fibronectin did not change. This is the first study demonstrating the different reactions induced by different viruses, and the protective effects of DXM on the EBV-induced EMT in the A549 cells by partially inhibiting the IL-13 pathway. These findings suggest a novel mechanism, by which DXM or anti-IL-13 may delay the progression of pulmonary fibrosis by preventing the progress of EBV-induced EMT.

PLA2G10 facilitates the cell-cycle progression of soft tissue leiomyosarcoma cells at least by elevating cyclin E1/CDK2 expression.

Soft tissue leiomyosarcoma (STLMS) is a major histological subtype of adult sarcoma. Although the molecular mechanisms ofLMS have been gradually revealed, no valid therapeutic targets have been identified. In this study, we performed a systematic screening to explore relapse-associated genes in STLMS, using data from The Cancer Genome Atlas-Sarcoma (TCGA-SARC). Then, we investigated the functional role of the gene with the best relapse-prediction value in STLMS by both in-vitro and in-vivo studies. Results showed that AMH and PLA2G10 were two genes with area under curve (AUC) values higher than 0.80 in ROC analysis when detecting relapse. Patients in the high AMH or PLA2G10 expression group had significantly worse relapse-free survival (RFS) compared to the respective low expression group. PLA2G10 was highly expressed in STLMS, but not in other sarcoma subtypes. PLA2G10 overexpression promoted SK-LMS-1 cell growth and G1/S transition, while PLA2G10 knockdown slowed the growth and resulted in G1 phase arrest. PLA2G10 overexpression markedly increased the expression of CDK2 and cyclin E1, but did not influence CDK4, CDK6, cyclin D1, CDK1 or cyclin A expression. PLA2G10 overexpression enhanced SK-LMS-1 cell-derived xenograft tumor growth in nude mice, while PLA2G10 inhibition slowed the growth. Mutation of two critical catalyzing amino acid residues (p.H88A and p.D89A) abrogated the capability of PLA2G10 to catalyze the production of arachidonic acid (AA), and also canceled the regulatory effects on cyclin E1 and CDK2 expression, as well as G1/S transition. In conclusion, PLA2G10 was a specific relapse-associated gene in STLMS. It facilitated the cell-cycle progression of STLMS cells at least by elevating the expression of cyclin E1 and CDK2. The hydrolytic activity was crucial for its oncogenic properties.