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1.
Food Chem ; 463(Pt 4): 140976, 2024 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-39362089

RESUMO

Oleogels have attracted considerable attention due to their excellent viscoelasticity and high content of polyunsaturated fatty acid. This study explored the potential of Zein/(-)-epigallocatechin-3-gallate/Ca2+ complexes oleogels loaded with lycopene as potential substitute for solid fats in biscuit formulations. Utilizing an emulsion-templated method, oleogels were prepared and characterized for visual appearance, droplet size, microstructure, and rheological properties. The incorporation of lycopene indicated a dose-dependent effect on these characteristics, achieving optimal properties at a concentration of 0.3 mg/mL. At this concentration, oleogels exhibited higher encapsulation efficiency (> 90 %), lower oil loss (< 2 %), and denser network structures. Rheological analysis highlighted the shear-thinning behavior, gel-like structure, and thixotropic recovery of oleogels. Substituting of margarine with lycopene-loaded oleogels in biscuits yielded products with regular appearance, uniform color, and potential health benefits, demonstrating the viability of these oleogels as a healthier alternative to traditional solid fats in baking.

2.
Food Chem ; 463(Pt 1): 141051, 2024 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-39241419

RESUMO

In this study, the self-assembly mechanism of Zein/(-)-epigallocatechin-3-gallate/polyethylene glycol (Zein/EGCG/PEG) composite nanoparticles and their interface adsorption behavior at the oil-water interface were investigated by coarse-grained molecular dynamics simulation. Fourier transform infrared spectroscopy and conformation analysis demonstrated that there were electrostatic and hydrogen bond interactions between Zein and EGCG, physical entanglement between PEG and Zein, and hydrogen bond interaction between EGCG and PEG. The nanoparticles accumulated at the oil-water interface, and there was an obvious interface layer between oil phase and water phase, as indicated by confocal laser scanning microscope and scanning electron microscope. The adsorbing of Zein/EGCG/PEG nanoparticles at the oil-water interface was confirmed by coarse-grained molecular dynamics simulation. Further findings confirmed that Zein/EGCG/PEG nanoparticles could serve as stabilizers for oleogels with self-supporting structure, viscoelastic solid behavior and temperature response characteristics. The current research offered a novel approach to enhance protein interface characteristics and create food-grade emulsifiers and oleogelators.

3.
Sci Rep ; 14(1): 14848, 2024 06 27.
Artigo em Inglês | MEDLINE | ID: mdl-38937539

RESUMO

This study aimed to assess the safety and efficacy of interventional embolization in cirrhotic patients with refractory hepatic encephalopathy (HE) associated with large spontaneous portosystemic shunts (SPSS). Inverse probability of treatment weighting (IPTW) was employed to minimize potential bias. A total of 123 patients were included in this study (34 in the embolization group and 89 in the control group). In the unadjusted cohort, the embolization group demonstrated significantly better liver function, a larger total area of SPSS, and a higher percentage of patients with serum ammonia levels > 60 µmol/L and the presence of hepatocellular carcinoma (HCC) (all P < 0.05). In the IPTW cohort, baseline characteristics were comparable between the two groups (all P > 0.05). Patients in the embolization group exhibited significantly longer HE-free survival compared to the control group in both the unadjusted and IPTW cohorts (both P < 0.05). Subsequent subgroup analyses indicated that patients with serum ammonia level > 60 µmol/L, hepatopetal flow within the portal trunk, the presence of solitary SPSS, a baseline HE grade of II, and the absence of HCC at baseline showed statistically significant benefit from embolization treatment (all P < 0.05). No early procedural complications were observed in the embolization group. The incidence of long-term postoperative complications was comparable to that in the control group (all P > 0.05). Hence, interventional embolization appears to be a safe and effective treatment modality for cirrhotic patients with refractory HE associated with large SPSS. However, the benefits of embolization were discernible only in a specific subset of patients.


Assuntos
Embolização Terapêutica , Encefalopatia Hepática , Cirrose Hepática , Humanos , Encefalopatia Hepática/terapia , Encefalopatia Hepática/etiologia , Masculino , Feminino , Embolização Terapêutica/métodos , Pessoa de Meia-Idade , Cirrose Hepática/complicações , Cirrose Hepática/terapia , Idoso , Resultado do Tratamento , Neoplasias Hepáticas/terapia , Neoplasias Hepáticas/complicações , Carcinoma Hepatocelular/terapia , Carcinoma Hepatocelular/complicações , Estudos Retrospectivos , Amônia/sangue
4.
Int J Biol Macromol ; 256(Pt 1): 128307, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37992941

RESUMO

Films with simultaneously excellent mechanical and anti-fog properties are of great importance for food packaging. A novel strategy is described here to prepare long-lasting anti-fog film with antibacterial and antioxidant capabilities via a simple, green approach. The CMC (carboxymethyl chitosan) gel was integrated with CNF/TA (cellulose nanofibers/tannic acid) composite solution based on layer-by-layer assembly to form a membrane with a bilayer structure. The anti-fog performance of the bilayer film could be adjusted by regulating the CNF/TA layer thickness. On the whole, the developed anti-fog film had high mechanical strength and excellent UV shielding properties, as well as good antibacterial and antioxidant properties, and could be non-fogging for a long time under water vapor (40 °C). The effect of double layer anti-fog film (3%CmFT-3) on the fresh-keeping effect of white Hypsizygus marmoreus was compared at room temperature (28 °C) with commercially available anti-fog PVC film. The results showed that the bilayer anti-fog film could effectively prevent the generation of fog, delay the Browning, inhibit mildew, improve the overall acceptability, and effectively extend the shelf life of white Hypsizygus marmoreus. This biomass-based anti-fog film offers great potential for the development of multifunctional green food packaging.


Assuntos
Agaricales , Quitosana , Nanofibras , Polifenóis , Quitosana/farmacologia , Quitosana/química , Celulose/farmacologia , Celulose/química , Nanofibras/química , Antioxidantes/farmacologia , Antibacterianos/farmacologia , Antibacterianos/química , Embalagem de Alimentos
5.
J Food Sci ; 87(1): 80-93, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34935129

RESUMO

Proteins, one of the vital nutritional compounds sensitive to the environment, can be modified by interaction with polyphenols. Ultrasonication has been applied for enhancing the functional properties of proteins. In this study, the interactions of gliadin (G) and rutin (R) in the absence and presence of ultrasonication (0, 150, 300, 450, and 600 W) for 20 min were investigated, with a focus on the properties of emulsions prepared by G-R complexes. Ultrasonication improved the interaction, which increased the content of ß-type secondary structure. Ultrasonication at 450 W increased the particle size of the conjugates. For Pickering emulsions, treating the covering of R on G with ultrasonication improves the stability of the G-based emulsion significantly, owing to the strong films formed on the oil-water interfaces. The G-R complexes treated at 450 W ultrasonication formed emulsions that showed higher potential and storage modulus (G') and denser microstructures than those of the untreated emulsions. Nevertheless, ultrasound treatment at 600 W weakened the emulsion properties that were stabilized by the conjugates. Ultrasound combined R was shown to be a potential processing technology for changing the protein structure and producing stable emulsions. PRACTICAL APPLICATION: The interactions between proteins and polyphenols are able to preserve the stability of the functional compounds, allow targeted and controlled release, and improve the texture of these complexes employed in the food industry. Improvements in the functional characteristics of the protein-polyphenol complexes so that they possess high emulsifying stability during food processing is a crucial factor for employing them in the food industry. Therefore, the aim of this research is using a soluble complex of gliadin-rutin for the development of its functional characteristics.


Assuntos
Gliadina , Nanopartículas , Emulsões , Tamanho da Partícula , Rutina , Água
6.
Food Chem ; 373(Pt B): 131489, 2022 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-34749091

RESUMO

Zein is potential in encapsulating and delivering polyphenols in food industry. Our study investigated the interaction mechanisms and structural changes of the interaction between ferulic acid (FA) and zein under different CaCl2 concentrations. Addition of CaCl2 resulted in amino acids micro-environment and structural changes of zein and zein/FA complex, which was dependent on different CaCl2 concentrations. At 0.5 mol/L CaCl2 concentration, zein/FA exhibited spherical particles with rough surfaces. Fourier transform infrared analysis showed the decrease of α-helix and ß-sheets contents accompanied by the increase of ß-turns and unordered coil contents. Molecular dynamics simulation demonstrated FA interacted with zein mainly through hydrogen bonds and hydrophobic force. These observations might contribute to the decreased surface hydrophobicity and digestibility of zein. Results provided a better understanding of the interaction between zein and other molecules, which might be helpful for the development of zein particles as functional materials to encapsulate and deliver bioactive compounds.


Assuntos
Zeína , Cloreto de Cálcio , Ácidos Cumáricos , Interações Hidrofóbicas e Hidrofílicas
7.
Food Funct ; 12(24): 12647-12658, 2021 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-34821891

RESUMO

Anthocyanins have anti-inflammatory, anticarcinogenic and antioxidant properties and anti-aging effects as well as potential application as pigments. The metabolism of anthocyanins in fermented food has attracted increasing attention. However, the effect of lactic acid bacteria (LAB) fermentation on its anti-aging activity remains mostly unknown. The current study aimed to investigate the compositions, antioxidant activities and anti-aging effect of fermented purple sweet potato anthocyanins (FSPA) on aging Caenorhabditis elegans compared to raw purple sweet potato anthocyanins (PSPA). Results showed that anthocyanins were degraded into more bioavailable phenolic acids by Weissella confusa fermentation. PSPA and FSPA can extend the lifespan of C. elegans by 26.7% and 37.5%, respectively, through improving the activity of antioxidant enzymes as well as decreasing MDA content, ROS levels and lipofuscin accumulation. Pretreatment of the worms with PSPA and FSPA induced their potential to resist to thermal tolerance and oxidative stress, and FSPA exerted a higher anti-stress effect than PSPA. Moreover, FSPA supplementation upregulated the mRNA expressions of genes daf-16, hsp-16.2, sir-2.1, skn-1 and sod-3 and downregulated the expression of daf-2 in the nematodes, whereas PSPA only induced the increase in the expressions of sir-2.1, skn-1 and sod-3. Overall, FSPA can improve stress resistance and extend the lifespan of C. elegans by both insulin/IGF-1 signaling pathway and dietary restriction pathway, providing a theoretical basis for the application of PSPA in fermented food as functional pigments.


Assuntos
Envelhecimento/efeitos dos fármacos , Antocianinas/farmacologia , Caenorhabditis elegans/efeitos dos fármacos , Ipomoea batatas/metabolismo , Extratos Vegetais/farmacologia , Animais , Fermentação
8.
Int J Pharm ; 602: 120650, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33957265

RESUMO

Curcumin (Cur), a hydrophobic active pharmaceutical ingredient with high anticancer activity, has poor water solubility and low bioavailability. Although many delivery systems have been developed to improve their bioavailability, some limitation such as low drug loading efficiency and poor stability are still remained. The metal-polyphenol networks (MPNs) delivery system designed in this subject solved above problems and effectively improved the anticancer activity of Cur. The synthesized Cur@EGCG-Fe(III) is consisting of epigallocatechin gallate (EGCG), iron chloride (FeCl3) and Cur, and the well-designed structure endow Cur@EGCG-Fe(III) high loading efficiency, good water solubility and stability. After the Cur@EGCG-Fe(III) nanoparticles were internalized by MCF-7 cells, the Cur could be released in endo/lysosomal microenvironment (pH = 5.0), and the Cur delivery in the deep tumor could be realized. The distribution of Cur@EGCG-Fe(III) in MCF-7 cells was analyzed by laser confocal, and Cur@EGCG-Fe(III) could effectively deliver more Cur into MCF-7 cells in comparison with free Cur. In addition, the results of flow cytometry and western blot further indicated that Cur@EGCG-Fe(III) had a stronger ability to induce apoptosis than free Cur. Transwell cell migration and invasion experiments showed that Cur and EGCG-Fe(III) had a synergistic effect in inhibiting MCF-7 cell migration and invasion. In vitro hemolysis and in vivo experiments showed that the Cur@EGCG-Fe(III) had negligible effect on the blood environment and a great tumor-inhibition efficacy, indicating that the MPNs delivery system had a good blood compatibility and antitumor activity. Our results indicated that MPNs-coated Cur nanoparticle could be a new form of Cur delivery system for anticancer application.


Assuntos
Antineoplásicos , Curcumina , Nanopartículas , Sistemas de Liberação de Medicamentos , Compostos Férricos , Humanos , Células MCF-7 , Polifenóis , Solubilidade
9.
Foods ; 11(1)2021 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-35010176

RESUMO

Ulcerative colitis (UC) is a chronic and nonspecific inflammatory disease of the colon and rectum, and its etiology remains obscure. Cherry polyphenols showed potential health-promoting effects. However, both the protective effect and mechanism of cherry polyphenols on UC are still unclear. This study aimed to investigate the potential role of the free polyphenol extract of cherry in alleviating UC and its possible mechanism of action. Our study revealed that the free polyphenol extract of cherry management significantly alleviated UC symptoms, such as weight loss, colon shortening, the thickening of colonic mucous layer, etc. The free polyphenol extract of cherry treatment also introduced a significant reduction in levels of malondialdehyde (MDA), myeloperoxidase (MPO) and nitric oxide (NO), while causing a significant elevation in levels of catalase (CAT), glutathione (GSH-Px), superoxide dismutase (SOD), as well as the downregulation of pro-inflammatory cytokines. This indicated that such positive effects were performed through reducing oxidative damage or in a cytokine-specific manner. The immunofluorescence analysis of ZO-1 and occludin proteins declared that the free polyphenol extract of cherry had the potential to prompt intestinal barrier function. The reduced expression levels of ß-catenin, c-myc, cyclin D1 and GSK-3ß suggested that the cherry extract performed its positive effect on UC by suppressing the Wnt/ß-ctenin pathway. This finding may pave the way into further understanding the mechanism of cherry polyphenols ameliorating ulcerative colitis.

10.
Carbohydr Polym ; 201: 464-470, 2018 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-30241842

RESUMO

Emulsified oily wastewater threated to human health seriously, and traditional technologies are unable to separate the emulsion for the small size of oil droplets. Membranes with special wettability are considered as an efficient way for oil/water emulsions separation, but challenges remain in terms of high material costs, complex processing, and easy to be fouled. Herein, novel self-cleanable membranes composed of nanocellulose and TiO2 nanoparticles were developed through a facile and sustainable vacuum-assisted filtration process. TiO2 nanoparticles in situ generated on the surface of tunicate cellulose nanocrystals (TCNC), a kind of nanocellulose, by hydrolysis of titanium oxysulfate. The prepared nanocomposite membrane showed hierarchical architecture, high roughness, superhydrophilicity and underwater oleophobicity. After UV-light irradiation, the resulting membrane exhibited improved underwater oil contact angles and water fluxes, beneficial for the selective oil/water emulsions separation. Importantly, the TiO2/nanocellulose membrane could rapidly degrade contaminant (oleic acid) under UV-light irradiation, suggesting excellent self-cleaning property. This works provided a facile strategy for development of self-cleanable membrane from nanocellulose for oil/water emulsion separation.

11.
J Proteomics ; 185: 39-50, 2018 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-29953961

RESUMO

Physical exercise has been reported to increase neurotrophin in brain tissues as hippocampus as well as increased neurotrophic level peripherally in blood plasma and might have an effect on/or affect molecular processes of energy metabolism (and homeostasis). In this study, using quantitative proteomic analysis, we obtained a plasma protein profile from the rat with long-term moderate exercise. A total of 752 proteins were identified in the plasma. Among them, 54 proteins were significant up-regulated and 47 proteins were down-regulated in the plasma of exercise group compared with the control group. Bioinformatic analyses showed that these altered proteins are widely involved in multiple biological processes, molecular functions and cellular components, which connect with 11 signaling pathways. Interestingly, 5 up-regulated proteins Rap1b, PTPN11, ARHGDIA, Cdc42 and YWHAE, confirmed by Western blots, are involved in the neurotrophin signaling pathway which shows the lowest P value among the identified pathways. Further analyses showed that the 5 neurotrophin-signaling-pathway-related proteins participate in two important protein-protein interaction networks associated to cell survival and apoptosis, axonal development, synapse formation and plasticity. This study provides an exercise-induced plasma protein profile, suggesting that long-term exercise enhances the proteins involved in neurotrophin signaling pathway which may contribute to health benefit. SIGNIFICANCE: Physical activity contributes to myriad benefits on body health across the lifespan. The changes in plasma proteins after chronic moderate exercise may be used as biomarkers for health and may also play important roles in increase of cardiovascular fitness, enhancement of immune competence, prevention of obesity, decrease of risk for neurological disorders, cancer, stroke, diabetes and other metabolic disorders. Using a TMT-based proteomic method, this study identified 101 altered proteins in the plasma of rats after long-term moderate treadmill running, which may provide novel biomarkers for further investigation of the underlying mechanism of physical exercise. We confirmed that exercise enhances 5 proteins of the neurotrophin signaling pathway that may contribute to health benefits.


Assuntos
Proteínas Sanguíneas/análise , Fatores de Crescimento Neural/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Condicionamento Físico Animal/métodos , Proteômica/métodos , Animais , Proteínas Sanguíneas/química , Proteínas Sanguíneas/metabolismo , Estudos de Casos e Controles , Cromatografia Líquida , Masculino , Neurogênese/fisiologia , Plasma/química , Plasma/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/fisiologia , Espectrometria de Massas em Tandem , Fatores de Tempo
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