RESUMO
LC-UV/MS-based metabolomic analysis of the Hawaiian endophytic fungus Paraphaeosphaeria neglecta FT462 led to the identification of four unique mercaptolactated γ-pyranol-γ-lactams, paraphaeosphaerides E-H (1-4) together with one γ-lactone (5) and the methyl ester of compound 2 (11). The structures of the new compounds (1-5 and 11) were elucidated through the analysis of HRMS and NMR spectroscopic data. The absolute configuration was determined by chemical reactions with sodium borohydride, hydrogen peroxide, α-methoxy-α-(trifluoromethyl)phenylacetyl chlorides (Mosher reagents), and DP4 + NMR calculations. All the compounds were tested against STAT3, A2780 and A2780cisR cancer cell lines, E. coli JW2496, and NF-κB. Compounds 1 and 3 strongly inhibited NF-κB with IC50 values of 7.1 and 1.5 µM, respectively.
Assuntos
Ascomicetos/química , Lactamas/química , Lactamas/farmacologia , Pironas/química , Safrol/análogos & derivados , Sulfetos/química , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Safrol/químicaRESUMO
Five previously undescribed ambuic acid derivatives, pestallic acids A-E and three known analogs were isolated from the cultured broth of Pestalotiopsis sp. FT172. The structures of the pestallic acids A-E were determined through the analysis of HRMS and NMR spectroscopic data. The absolute configurations (ACs) of pestallic acids B-E were assigned by comparison of the experimental electric circular dichroism (ECD) spectra or the optical rotations with those in the literature. All compounds were tested against A2780 and cisplatin resistant A2780 (A2780CisR) cell lines. Pestallic acid E and (+)-ambuic acid showed potent activities with IC50 values from 3.3 to 17.0 µM.
Assuntos
Antineoplásicos Fitogênicos/química , Cicloexanonas/química , Xylariales/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Cicloexanonas/isolamento & purificação , Endófitos/química , Havaí , Humanos , Estrutura Molecular , Primulaceae/microbiologiaRESUMO
Three unusual polyketide-sesquiterpene metabolites peyronellins A-C (1-3), along with the new epoxyphomalin analog 11-dehydroxy epoxyphomalin A (4), have been isolated from the endophytic fungus Peyronellaea coffeae-arabicae FT238, which was isolated from the native Hawaiian plant Pritchardia lowreyana. The structures of compounds 1-4 were characterized based on NMR and MS spectroscopic analysis. The absolute configuration (AC) of the compounds was determined by electronic circular dichroism (ECD). Compound 4 showed antiproliferative activity with an IC50 of 0.5 µM against OVCAR3, and it also strongly inhibited Stat3 at 5 µM.
Assuntos
Antineoplásicos/isolamento & purificação , Arecaceae/microbiologia , Ascomicetos/química , Proliferação de Células/efeitos dos fármacos , Endófitos/química , Terpenos/isolamento & purificação , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Terpenos/farmacologiaRESUMO
The potential anti-tumor agent wentilactones were produced by a newly isolated marine fungus Aspergillus dimorphicus. This fungus was derived from deep-sea sediment and identified by polyphasic approach, combining phenotypic, molecular, and extrolite profiles. However, wentilactone production was detected only under static cultures with very low yields. In order to improve wentilactone production, culture conditions were optimized using the response surface methodology. Under the optimal static fermentation conditions, the experimental values were closely consistent with the prediction model. The yields of wentilactone A and B were increased about 11-fold to 13.4 and 6.5 mg/L, respectively. The result was further verified by fermentation scale-up for wentilactone production. Moreover, some small-molecule elicitors were found to have capacity of stimulating wentilactone production. To our knowledge, this is first report of optimized production of tetranorlabdane diterpenoids by a deep-sea derived marine fungus. The present study might be valuable for efficient production of wentilactones and fundamental investigation of the anti-tumor mechanism of norditerpenoids.
Assuntos
Antineoplásicos/isolamento & purificação , Aspergillus/metabolismo , Sedimentos Geológicos/microbiologia , Compostos Heterocíclicos de 4 ou mais Anéis/isolamento & purificação , Aspergillus/isolamento & purificação , Meios de Cultura , FermentaçãoRESUMO
Bioassay-guided fractionation of a culture extract of Beauveria felina EN-135, an entomopathogenic fungus isolated from a marine bryozoan, led to the isolation of a new cyclodepsipeptide, iso-isariin D (1); two new O-containing heterocyclic compounds that we have named felinones A and B (2 and 3); and four known cyclodepsipeptides (4-7). The structures were elucidated via spectroscopic analysis, and the absolute configurations of 1 and 2 were determined using single-crystal X-ray diffraction and CD, respectively. All isolated compounds were evaluated for antimicrobial activity and brine-shrimp (Artemia salina) lethality.
Assuntos
Beauveria/química , Depsipeptídeos/química , Compostos Heterocíclicos/química , Animais , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Antineoplásicos/farmacologia , Artemia , Bactérias/efeitos dos fármacos , Depsipeptídeos/isolamento & purificação , Depsipeptídeos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Fungos/efeitos dos fármacos , Compostos Heterocíclicos/isolamento & purificação , Compostos Heterocíclicos/farmacologia , Testes de Sensibilidade Microbiana , Modelos Moleculares , Conformação MolecularRESUMO
Three new indolediketopiperazine peroxides, namely, 24-hydroxyverruculogen (1), 26-hydroxyverruculogen (2), and 13-O-prenyl-26-hydroxyverruculogen (3), along with four known homologues (4-7), were isolated and identified from the culture extract of the marine sediment-derived fungus Penicillium brefeldianum SD-273. Their structures were determined based on the extensive spectroscopic analysis and compound 1 was confirmed by X-ray crystallographic analysis. The absolute configuration of compounds 1-3 was determined using chiral HPLC analysis of their acidic hydrolysates. Each of the isolated compounds was evaluated for antibacterial and cytotoxic activity as well as brine shrimp (Artemia salina) lethality.
Assuntos
Dicetopiperazinas/farmacologia , Indóis/farmacologia , Penicillium/metabolismo , Peróxidos/farmacologia , Animais , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Antineoplásicos/farmacologia , Artemia/efeitos dos fármacos , Linhagem Celular Tumoral , Cromatografia Líquida de Alta Pressão , Cristalografia por Raios X , Dicetopiperazinas/química , Dicetopiperazinas/isolamento & purificação , Sedimentos Geológicos/microbiologia , Humanos , Indóis/química , Indóis/isolamento & purificação , Peróxidos/química , Peróxidos/isolamento & purificação , Prenilação , Análise Espectral , Testes de Toxicidade/métodosRESUMO
Sumalarins A-C (1-3), the new and rare examples of sulfur-containing curvularin derivatives, along with three known analogues (4-6), were isolated and identified from the cytotoxic extract of Penicillium sumatrense MA-92, a fungus obtained from the rhizosphere of the mangrove Lumnitzera racemosa . Their structures were established by detailed interpretation of NMR and MS data, and compound 1 was confirmed by X-ray crystallographic analysis. Compounds 1-3 and 5 showed potent cytotoxicity against some of the tested tumor cell lines. Sulfur substitution at C-11 or a double bond at C-10 significantly increased the cytotoxic activities of the curvularin analogues.
Assuntos
Antineoplásicos/isolamento & purificação , Antineoplásicos/farmacologia , Combretaceae/microbiologia , Macrolídeos/isolamento & purificação , Macrolídeos/farmacologia , Penicillium/química , Enxofre/análise , Zearalenona/análogos & derivados , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antineoplásicos/química , Cristalografia por Raios X , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Macrolídeos/química , Conformação Molecular , Estrutura Molecular , Relação Estrutura-Atividade , Zearalenona/química , Zearalenona/isolamento & purificação , Zearalenona/farmacologiaRESUMO
Six new 4-phenyl-3,4-dihydroquinolone derivatives (1-6) along with the related aflaquinolone A (7) were isolated and identified from the cultures of Aspergillus nidulans MA-143, an endophytic fungus obtained from the fresh leaves of the marine mangrove plant Rhizophora stylosa. Their structures including absolute configurations were determined by spectroscopic analysis and electronic circular dichroism experiments, and the structure of compound 1 was confirmed by single-crystal X-ray crystallographic analysis. In bioscreening experiments, none of the isolated compounds showed potent antibacterial or cytotoxic activity. However, compounds 2, 3, and 7 exhibited lethality against brine shrimp (Artemia salina), with LD50 values of 7.1, 4.5, and 5.5 µM, respectively.
Assuntos
Aspergillus nidulans/química , Quinolonas/isolamento & purificação , Rhizophoraceae/microbiologia , Animais , Artemia/efeitos dos fármacos , Dicroísmo Circular , Cristalografia por Raios X , Ensaios de Seleção de Medicamentos Antitumorais , Escherichia coli/efeitos dos fármacos , Feminino , Células HL-60 , Humanos , Células K562 , Testes de Sensibilidade Microbiana , Conformação Molecular , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Folhas de Planta/microbiologia , Quinolonas/química , Quinolonas/farmacologia , Staphylococcus aureus/efeitos dos fármacosRESUMO
Five new anthranilic acid derivatives, penipacids A-E (1-5), together with one known analogue (6), which was previously synthesized, were characterized from the ethyl acetate extract of the marine sediment-derived fungus Penicillium paneum SD-44. Their structures were elucidated mainly by extensive NMR spectroscopic and mass spectrometric analysis. The cytotoxicity and antimicrobial activity of the isolated compounds were evaluated. Compounds 1, and 5 exhibited inhibitory activity against human colon cancer RKO cell line, while compound 6 displayed cytotoxic activity against Hela cell line.
Assuntos
Anti-Infecciosos/farmacologia , Antineoplásicos/farmacologia , Penicillium/química , ortoaminobenzoatos/farmacologia , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Neoplasias do Colo/tratamento farmacológico , Neoplasias do Colo/patologia , Sedimentos Geológicos , Células HeLa , Humanos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Ácido Micofenólico/análogos & derivados , Ácido Micofenólico/química , Ácido Micofenólico/isolamento & purificação , Ácido Micofenólico/farmacologia , ortoaminobenzoatos/química , ortoaminobenzoatos/isolamento & purificaçãoRESUMO
Penicillium sp. MA-37, which was obtained from the rhizospheric soil of the mangrove plant Bruguiera gymnorrhiza, exhibited different chemical profiles in static and shaken fermentation modes. Three new meroterpenoid derivatives, 4,25-dehydrominiolutelide B (1), 4,25-dehydro-22-deoxyminiolutelide B (2), and isominiolutelide A (3), together with three known ones were characterized from its static fermentation, while three new diphenyl ether derivatives, namely, Δ(1('),3('))-1'-dehydroxypenicillide (4), 7-O-acetylsecopenicillide C (5), and hydroxytenellic acid B (6), along with five related metabolites were isolated from the shaken culture. The structures of these compounds were elucidated on the basis of spectroscopic analysis, and the structure of compound 2 was confirmed by X-ray crystallographic analysis. The absolute configurations of 1-3 and 6 were determined by ECD and modified Mosher's method, respectively. All isolated compounds were evaluated for brine shrimp lethality and antibacterial activity.
Assuntos
Penicillium/química , Éteres Fenílicos/isolamento & purificação , Rhizophoraceae/microbiologia , Terpenos/isolamento & purificação , Animais , Antibacterianos/química , Artemia/efeitos dos fármacos , Cristalografia por Raios X , Ensaios de Seleção de Medicamentos Antitumorais , Fermentação , Biologia Marinha , Testes de Sensibilidade Microbiana , Estrutura Molecular , Éteres Fenílicos/química , Éteres Fenílicos/farmacologia , Microbiologia do Solo , Terpenos/química , Terpenos/farmacologiaRESUMO
Bioassay-guided isolation of a fungal strain Nigrospora sp. MA75, an endophytic fungus obtained from the marine semi-mangrove plant Pongamia pinnata, which was fermented on three different culture media, resulted in the isolation and identification of seven known compounds, 2, 3, and 5-9, from a medium containing 3.5% NaCl, while a new compound, 2,3-didehydro-19α-hydroxy-14-epicochlioquinone B (10) was obtained from the medium containing 3.5% NaI. In addition, two new griseofulvin derivatives, 6-O-desmethyldechlorogriseofulvin (1) and 6'-hydroxygriseofulvin (4), were isolated and identified from the rice solid medium. Dechlorogriseofulvin (2) and griseofulvin (3) were the major components in fermentation extracts of all these culture media, while compounds 1 and 4, 5 and 6, and 10 were only present in the extract of respective culture medium. The structures of these compounds were elucidated by detailed spectroscopic analysis, and the absolute configuration of 1 was determined by CD measurement. Compounds 9 and 10 exhibited antibacterial activities toward five tested bacterial strains, while compounds 5, 6, and 8 selectively inhibited MRSA, E. coli, and S. epidermidis, and compound 3 showed moderate activity against V. mali and S. solani. Moreover, compound 10 potently inhibited the growth of MCF-7, SW1990, and SMMC7721 tumor cell lines with IC(50) values of 4, 5, and 7 µg/ml, respectively.
Assuntos
Ascomicetos/metabolismo , Animais , Antibacterianos , Ascomicetos/química , Ascomicetos/classificação , Linhagem Celular Tumoral , Cromatografia Líquida de Alta Pressão , Dicroísmo Circular , Simulação por Computador , Meios de Cultura , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Concentração Inibidora 50 , Biologia Marinha , Estrutura MolecularRESUMO
Bioassay-guided fractionation of the culture extract of Aspergillus wentii EN-48, an endophytic fungus isolated from an unidentified marine brown algal species of the genus Sargassum, led to the isolation of three new tetranorlabdane diterpenoids, asperolides A-C (1-3), and five related derivatives (4-8). The structures of these compounds were established on the basis of spectroscopic interpretation, and compound 1 was confirmed by X-ray crystallographic analysis. The absolute configuration of 1 was determined by application of the modified Mosher's method. An X-ray structure for wentilactone B (6) is also reported. Compounds 1-8 were evaluated for cytotoxic and antibacterial activities.
Assuntos
Aspergillus/química , Diterpenos/isolamento & purificação , Phaeophyceae/química , Ampicilina/farmacologia , Cristalografia por Raios X , Diterpenos/química , Diterpenos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Células HeLa , Humanos , Biologia Marinha , Testes de Sensibilidade Microbiana , Conformação Molecular , Estrutura Molecular , Ressonância Magnética Nuclear BiomolecularRESUMO
Eight new α-pyrone derivatives, namely, nigerapyrones A-E (1-5) and nigerapyrones F-H (8-10), along with two known congeners, asnipyrones B (6) and A (7), were isolated from Aspergillus niger MA-132, an endophytic fungus obtained from the fresh tissue of the marine mangrove plant Avicennia marina. The structures of these compounds were elucidated on the basis of spectroscopic analysis. The undescribed geometries of the trisubstituted double bonds (C-9 and C-11) for asnipyrone B (6) have now been explicitly determined, while the incorrect placement of the methyl group at C-5 of asnipyrone A (7) has now been revised at C-3. The cytotoxic activities of the isolated α-pyrone derivatives against eight tumor cell lines as well as antimicrobial activities against two bacteria and four plant-pathogenic fungi of these compounds were evaluated. Compounds 2, 4, 5, and 7 showed weak cytotoxicity against some of the tested tumor cell lines.
Assuntos
Antineoplásicos/isolamento & purificação , Aspergillus niger/química , Pironas/isolamento & purificação , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antineoplásicos/química , Antineoplásicos/farmacologia , Avicennia/microbiologia , Ensaios de Seleção de Medicamentos Antitumorais , Células Hep G2 , Humanos , Biologia Marinha , Testes de Sensibilidade Microbiana , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Pironas/química , Pironas/farmacologia , EstereoisomerismoRESUMO
A novel triazole carboxylic acid, penipanoid A (1), two new quinazolinone alkaloids, penipanoids B (2) and C (3), and a very recently reported quinazolinone derivative (4) were isolated from the marine sediment-derived fungus Penicillium paneum SD-44. Their structures were elucidated by spectroscopic analysis, and the structure for 1 was confirmed by X-ray crystallographic analysis. Compound 1 represents the first example of a triazole derivative from marine sediment-derived fungi, and compound 2 is a rare quinazolinone derivative having a dihydroimidazole ring system. The cytotoxicity of compounds 1 and 4 and the antimicrobial activity of 1-4 were evaluated.
Assuntos
Alcaloides/isolamento & purificação , Antineoplásicos/isolamento & purificação , Imidazóis/isolamento & purificação , Penicillium/química , Quinazolinonas/isolamento & purificação , Triazóis/isolamento & purificação , Alcaloides/química , Alcaloides/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Ácidos Carboxílicos , Ensaios de Seleção de Medicamentos Antitumorais , Sedimentos Geológicos/química , Humanos , Imidazóis/química , Imidazóis/farmacologia , Testes de Sensibilidade Microbiana , Estrutura Molecular , Quinazolinonas/química , Quinazolinonas/farmacologia , Triazóis/química , Triazóis/farmacologiaRESUMO
Chemical investigation of Penicillium commune QSD-17, a fungus isolated from a marine sediment sample collected in the southern China Sea, yielded six new azaphilone derivatives, namely, comazaphilones A-F (1-6). The structures of these compounds were established on the basis of spectroscopic analysis. Attempts to define the absolute configuration of these azaphilones through investigation of Mosher's esters failed, possibly due to steric crowding at C-6 and C-7 and due to the degradation of these azaphilone derivatives under the reaction conditions. The inhibitory activities of the six azaphilones against four bacteria, one pathogenic fungus, and seven tumor cell lines were evaluated. Compounds 3-5 displayed potent inhibitory activity against several of these bacteria, while compounds 4-6 showed cytotoxic activity against human pancreatic tumor cell line SW1990. The preliminary SAR results indicated that the double bond at C-10 and the location of the orsellinic acid unit at C-6 in these azaphilones are important for the antibacterial activity and cytotoxicity, respectively. This is the first report of the isolation of azaphilone derivatives from a marine sediment-derived fungus.
Assuntos
Antibacterianos/isolamento & purificação , Antineoplásicos/isolamento & purificação , Benzopiranos/isolamento & purificação , Penicillium/química , Pigmentos Biológicos/isolamento & purificação , Antibacterianos/química , Antibacterianos/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Benzopiranos/química , Benzopiranos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Sedimentos Geológicos/microbiologia , Humanos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Pigmentos Biológicos/química , Pigmentos Biológicos/farmacologia , Relação Estrutura-AtividadeRESUMO
7-Nor-ergosterolide (1), a rare 7-norsteroid with an unusual pentalactone B-ring system, and two new steroid derivatives, 3ß,11α-dihydroxyergosta-8,24(28)-dien-7-one (2) and 3ß-hydroxyergosta-8,24(28)-dien-7-one (3), were characterized from the culture extract of Aspergillus ochraceus EN-31, an endophytic fungus isolated from the marine brown alga Sargassum kjellmanianum. In addition, nine known related steroids were isolated and identified. The structures of these compounds were established on the basis of extensive spectroscopic analysis. The absolute configuration of the new steroids (1-3) was determined by application of the modified Mosher's method. Compound 1, which represents the first example of a 7-nor-ergosteroid possessing a pentalactone B-ring system in a naturally occurring steroid, displayed cytotoxicity against NCI-H460, SMMC-7721, and SW1990 cell lines with IC(50) values of 5.0, 7.0, and 28.0 µg/mL, respectively. Compound 2 also displayed cytotoxicity against the SMMC-7721 cell line with an IC(50) value of 28.0 µg/mL.
Assuntos
Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Aspergillus ochraceus/química , Noresteroides/química , Noresteroides/isolamento & purificação , Antineoplásicos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Concentração Inibidora 50 , Biologia Marinha , Estrutura Molecular , Noresteroides/farmacologia , Ressonância Magnética Nuclear Biomolecular , Phaeophyceae/microbiologia , EstereoisomerismoRESUMO
Cytoglobosins A-G (1-7), seven new cytochalasan derivatives, along with two structurally related known compounds, isochaetoglobosin D (8) and chaetoglobosin F(ex) (9), were isolated and identified from the cultures of Chaetomium globosum QEN-14, an endophytic fungus derived from the marine green alga Ulva pertusa. The structures of the new natural products as well as their relative configurations were elucidated on the basis of 1D and 2D NMR spectra (COSY, HSQC, HMBC, and NOESY) and HRESIMS data. Cytoglobosins C and D (3 and 4) displayed cytotoxic activity against the A-549 tumor cell line.
Assuntos
Alcaloides Indólicos/isolamento & purificação , Animais , Chaetomium/química , Clorófitas/microbiologia , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Alcaloides Indólicos/química , Alcaloides Indólicos/farmacologia , Células KB , Leucemia P388 , Biologia Marinha , Camundongos , Estrutura Molecular , Ressonância Magnética Nuclear BiomolecularRESUMO
Penicillium chrysogenum QEN-24S, an endophytic fungus isolated from an unidentified marine red algal species of the genus Laurencia, displayed inhibitory activity against the growth of pathogen Alternaria brassicae in dual culture test. Chemical investigation of this fungal strain resulted in the isolation of four new (1-3 and 5) and one known (4) secondary metabolites. Their structures were identified as two polyketide derivatives penicitides A and B (1 and 2), two glycerol derivatives 2-(2,4-dihydroxy-6-methylbenzoyl)-glycerol (3) and 1-(2,4-dihydroxy-6-methylbenzoyl)- glycerol (4), and one monoterpene derivative penicimonoterpene (5). Penicitides A and B (1 and 2) feature a unique 10-hydroxy- or 7,10-dihydroxy-5,7-dimethylundecyl moiety substituting at C-5 of the α-tetrahydropyrone ring, which is not reported previously among natural products. Compound 5 displayed potent activity against the pathogen A. brassicae, while compound 1 exhibited moderate cytotoxic activity against the human hepatocellular liver carcinoma cell line.
Assuntos
Antifúngicos/farmacologia , Antineoplásicos/farmacologia , Penicillium chrysogenum/metabolismo , Alternaria/efeitos dos fármacos , Antifúngicos/química , Antifúngicos/isolamento & purificação , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Organismos Aquáticos , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Células HeLa , Células Hep G2 , Humanos , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Oceanos e Mares , Penicillium chrysogenum/química , Penicillium chrysogenum/genética , Rodófitas/microbiologiaRESUMO
A new alkaloid, daphenylline (1), with an unprecedented rearranged 22-nor-calyciphylline skeleton, was isolated from the fruits of Daphniphyllum longeracemosum. Its structure and stereochemistry were elucidated on the basis of spectroscopic and computational approaches. A plausible biosynthetic pathway of 1 was also proposed.
Assuntos
Alcaloides/química , Alcaloides/isolamento & purificação , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Saxifragaceae/química , Alcaloides/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Estrutura MolecularRESUMO
Three types with six new carboline alkaloids, trigonostemonines A-F (1- 6), were isolated from the roots and stems of Trigonostemon lii. Their structures were elucidated by spectroscopic analyses. It is the first time that beta-carboline alkaloids have been reported in Trigonostemon species. Trigonostemonine F (6) exhibited moderate cytotoxic activity against HL-60 with an IC (50) value of 16 microM.