Assuntos
Carcinoma de Células Escamosas/patologia , Neoplasias Cutâneas/patologia , Idoso de 80 Anos ou mais , Anticorpos Monoclonais/metabolismo , Carcinoma de Células Escamosas/metabolismo , Diagnóstico Diferencial , Granuloma/metabolismo , Granuloma/patologia , Humanos , Queratinas/imunologia , Masculino , Mucina-1/metabolismo , Neoplasias Cutâneas/metabolismoAssuntos
Tumor de Brenner/patologia , Carcinoma de Células de Transição/patologia , Neoplasias Primárias Múltiplas/patologia , Neoplasias Ovarianas/patologia , Neoplasias da Bexiga Urinária/patologia , Idoso , Proteína 1 de Troca de Ânion do Eritrócito/metabolismo , Tumor de Brenner/metabolismo , Tumor de Brenner/cirurgia , Carcinoma de Células de Transição/metabolismo , Carcinoma de Células de Transição/cirurgia , Cromogranina A/metabolismo , Cistectomia , Feminino , Humanos , Histerectomia , Proteínas de Membrana/metabolismo , Neoplasias Primárias Múltiplas/metabolismo , Neoplasias Primárias Múltiplas/cirurgia , Neoplasias Ovarianas/metabolismo , Neoplasias Ovarianas/cirurgia , Neoplasias da Bexiga Urinária/metabolismo , Neoplasias da Bexiga Urinária/cirurgiaAssuntos
Linfoma Folicular/patologia , Linfoma de Célula do Manto/patologia , Antígenos CD5/metabolismo , Cromossomos Humanos Par 11 , Cromossomos Humanos Par 14 , Cromossomos Humanos Par 18 , Ciclina D1/metabolismo , Diagnóstico Diferencial , Humanos , Antígeno Ki-67/metabolismo , Linfoma de Zona Marginal Tipo Células B/genética , Linfoma de Zona Marginal Tipo Células B/metabolismo , Linfoma de Zona Marginal Tipo Células B/patologia , Linfoma Folicular/genética , Linfoma Folicular/metabolismo , Linfoma Folicular/radioterapia , Linfoma de Célula do Manto/genética , Linfoma de Célula do Manto/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Pseudolinfoma/metabolismo , Pseudolinfoma/patologia , Translocação GenéticaRESUMO
OBJECTIVE: To study the value of combined use of paternally imprinted gene product p57(KIP2) immunohistochemistry and flow cytometry in the differential diagnosis of placental hydropic diseases. METHODS: A total of 32 cases of hydropic placenta with DNA polymorphism information were collected, and the genetic results were used as basis for the diagnosis of complete hydatidiform moles (CHM), partial hydatidiform moles (PHM) or hydropic abortions. All cases were examined by histology, p57(KIP2) immunohistochemical staining (EnVision method) and flow cytometry DNA ploidy analysis. The p57(KIP2) immunohistochemical staining and DNA ploidy results were compared with the genetic results. RESULTS: In CHM, p57(KIP2) negative rates were 95.2% (20/21), whereas all the 11 cases of non-CHM (7 cases PHM and 4 cases hydropic abortions) were positive (11/11). In 11 p57(KIP2) -positive cases, 7 cases with triploidy and 4 cases with diploidy by flow cytometry were proven to be PHM and hydropic abortions by genetic analysis, respectively. Overall, 96.9% (31/32) cases of hydropic placentas were correctly diagnosed by combined use of p57(KIP2) immunohistochemistry and flow cytometry. CONCLUSIONS: p57(KIP2) immunohistochemical negativity is a reliable index for the diagnosis of CHM. Combined flow cytometry DNA ploidy and p57(KIP2) immunohistochemistry are useful in the pathological differentiation of CHM, PHM and hydropic abortions.
Assuntos
Inibidor de Quinase Dependente de Ciclina p57/metabolismo , Mola Hidatiforme/diagnóstico , Neoplasias Uterinas/diagnóstico , Aborto Espontâneo/diagnóstico , Aborto Espontâneo/genética , Aborto Espontâneo/metabolismo , Adulto , DNA de Neoplasias/análise , Diagnóstico Diferencial , Diploide , Feminino , Citometria de Fluxo , Humanos , Mola Hidatiforme/genética , Mola Hidatiforme/metabolismo , Imuno-Histoquímica , Pessoa de Meia-Idade , Gravidez , Triploidia , Neoplasias Uterinas/genética , Neoplasias Uterinas/metabolismo , Adulto JovemAssuntos
Hiperplasia do Linfonodo Gigante/patologia , Sarcoma de Células Dendríticas Foliculares/patologia , Doenças Torácicas/patologia , Hiperplasia do Linfonodo Gigante/complicações , Hiperplasia do Linfonodo Gigante/metabolismo , Hiperplasia do Linfonodo Gigante/cirurgia , Sarcoma de Células Dendríticas Foliculares/complicações , Sarcoma de Células Dendríticas Foliculares/metabolismo , Sarcoma de Células Dendríticas Foliculares/cirurgia , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Receptores de Complemento 3b/metabolismo , Doenças Torácicas/complicações , Doenças Torácicas/metabolismo , Doenças Torácicas/cirurgia , Parede Torácica , Vimentina/metabolismoAssuntos
Omento/patologia , Neoplasias Peritoneais/patologia , Neoplasias Trofoblásticas/patologia , Adulto , Diagnóstico Diferencial , Feminino , Humanos , Queratina-18/metabolismo , Mucina-1/metabolismo , Omento/metabolismo , Neoplasias Peritoneais/metabolismo , Neoplasias Peritoneais/ultraestrutura , Gravidez , Neoplasias Trofoblásticas/metabolismo , Neoplasias Trofoblásticas/ultraestrutura , Tumor Trofoblástico de Localização Placentária/patologia , Vimentina/metabolismoAssuntos
Neoplasias Ósseas/patologia , Tumor Glômico/patologia , Tíbia/patologia , Adolescente , Neoplasias Ósseas/metabolismo , Neoplasias Ósseas/cirurgia , Diagnóstico Diferencial , Seguimentos , Tumor Glômico/metabolismo , Tumor Glômico/cirurgia , Humanos , Imuno-Histoquímica , Masculino , Melanoma/patologia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Tíbia/metabolismo , Tíbia/cirurgia , Vimentina/metabolismoRESUMO
Epithelioid angioleiomyoma is rare in the skin and subcutis. We report here an unusual case of epithelioid angioleiomyoma with prominent clear-cell change. Smooth-muscle differentiation was confirmed by immunostains. The extensive clear-cell change in epithelial cells and the marked hyalinization and calcification made the histologic differential diagnosis challenging. Epithelioid angioleiomyoma shares some histologic characteristics with glomus tumors and seems to be an intermediate entity between angioleiomyomas and glomus tumors.
Assuntos
Angiomioma/diagnóstico , Epiderme/patologia , Células Epitelioides/patologia , Tumor Glômico/diagnóstico , Neoplasias Cutâneas/diagnóstico , Actinas/análise , Adulto , Angiomioma/química , Angiomioma/patologia , Calcinose/diagnóstico , Diferenciação Celular , Desmina/análise , Diagnóstico Diferencial , Epiderme/química , Células Epitelioides/química , Feminino , Tumor Glômico/química , Tumor Glômico/patologia , Humanos , Hialina/metabolismo , Imuno-Histoquímica , Miócitos de Músculo Liso/patologia , Reticulina/análise , Proteínas S100/análise , Neoplasias Cutâneas/química , Neoplasias Cutâneas/patologia , Vacúolos/patologiaAssuntos
Neoplasias Ósseas/patologia , Sarcoma de Células de Langerhans/patologia , Tálus , Neoplasias Ósseas/metabolismo , Neoplasias Ósseas/cirurgia , Diagnóstico Diferencial , Feminino , Humanos , Imuno-Histoquímica , Sarcoma de Células de Langerhans/metabolismo , Sarcoma de Células de Langerhans/cirurgia , Pessoa de Meia-Idade , Proteínas S100/metabolismo , Vimentina/metabolismoRESUMO
OBJECTIVE: To investigate the clinicopathological features of pulmonary lymphangioleiomyomatosis (PLAM). METHODS: By means of HE and immunohistochemistry (SP method) studies, the clinical and pathological features of 5 PLAM cases were analyzed and the related literature reviewed. RESULTS: PLAM was a rare lung disease of unknown etiology and was restricted to females who were generally pre-menopausal. Pathological features showed abnormal smooth muscle cells (LAM cells) line the airways, lymphatics and blood vesssels leading to airflow obstruction and replacement of the lung parenchyma by cysts. LAM cells were positive for HMB45. Clinically the disease was categorized by dyspnoea, haemoptysis, recurrent pneumothoraces and chylous effusions. CONCLUSIONS: PLAM should be considered when recurrent pneumothorax, haemoptysis and dyspnoea occur in females. Pathologic examination of lung tissue biopsy is required for confirmation of PLAM diagnosis.
Assuntos
Neoplasias Pulmonares/patologia , Pulmão/patologia , Linfangioleiomiomatose/patologia , Actinas/metabolismo , Adulto , Antígenos de Neoplasias , Biópsia , Diagnóstico Diferencial , Feminino , Humanos , Imuno-Histoquímica , Pulmão/diagnóstico por imagem , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/metabolismo , Linfangioleiomiomatose/diagnóstico por imagem , Linfangioleiomiomatose/metabolismo , Metaloproteinase 2 da Matriz/metabolismo , Antígenos Específicos de Melanoma , Pessoa de Meia-Idade , Proteínas de Neoplasias/metabolismo , Tomografia Computadorizada por Raios XAssuntos
Doença de Hodgkin/patologia , Neoplasias Pulmonares/patologia , Pulmão/patologia , Linfonodos/patologia , Adulto , Diagnóstico Diferencial , Feminino , Histiocitoma Fibroso Maligno/imunologia , Histiocitoma Fibroso Maligno/patologia , Histiocitose de Células de Langerhans/imunologia , Histiocitose de Células de Langerhans/patologia , Histiocitose Sinusal/imunologia , Histiocitose Sinusal/patologia , Doença de Hodgkin/imunologia , Humanos , Antígeno Ki-1/metabolismo , Antígenos CD15/metabolismo , Pulmão/imunologia , Neoplasias Pulmonares/imunologia , Linfonodos/imunologiaRESUMO
OBJECTIVE: To investigate the expression of thymosin beta10 (Tbeta10) and related changes of actin filament organization in human tumor cell lines with different metastatic potential. METHODS: Four groups of nine human tumor cell lines with different metastatic potential were analyzed for the expression of Tbeta10 mRNA detected by northern-blot and its peptide by immunohistochemical staining. The filamentous actin (F-actin) was stained with TRITC-phalloidin to detect changes in actin organization. RESULTS: In comparison with the non and/or weakly metastatic counterparts, Tbeta10 was upregulated in highly metastatic human lung cancer, malignant melanoma and breast cancer cell lines. TRITC-phalloidin staining revealed less actin bundles and a fuzzy network of shorter filaments in the highly metastatic tumor cells, while in the non and/or weakly metastatic cancer cell lines, there were thick and orderly arranged actin filaments. CONCLUSIONS: Tbeta10 levels correlate positively with the metastatic phenotype in human tumors currently examined. The increased metastatic potential of tumor cells is accompanied by the loss of F-actin and poorly organized actin skeleton. There is a consistent correlation between the elevated Tbeta10 expression and the disrupted actin skeleton.
Assuntos
Actinas/análise , Metástase Neoplásica , Timosina/análise , Northern Blotting , Linhagem Celular Tumoral , Humanos , Imuno-HistoquímicaRESUMO
BACKGROUND: To investigate the differential expression levels of thymosin beta 10 (T beta 10) and the corresponding changes of actin filament organization in human tumor cell lines with different metastatic potential. METHODS: Four groups of nine human tumor cell lines with different metastatic potential were analyzed for the amount of T beta 10 mRNAs by Northern blot and for their peptide expression levels by immunohistochemistry. The filamentous actin (F-actin) was observed by staining of TRITC-phalloidin to detect changes in actin organization. RESULTS: In comparison with non-/weakly metastatic counterparts, T beta 10 was upregulated in highly metastatic human lung cancer, malignant melanoma and breast cancer cell lines. Staining of TRITC-phalloidin revealed less actin bundles, a fuzzy network of shorter filaments and some F-actin aggregates in the highly metastatic tumor cells. Meanwhile, the actin filaments were robust and orderly arranged in the non-/weakly metastatic cancer cell lines. CONCLUSION: T beta 10 levels correlate positively with the metastatic capacity in human tumors currently examined. The increasing metastatic potential of tumor cells is accompanied by a loss of F-actin, poorly arranged actin skeleton organizations and presence of F-actin aggregates. There is a consistent correlation between the elevated T beta 10 expression and the disrupted actin skeleton.
Assuntos
Citoesqueleto de Actina/ultraestrutura , Metástase Neoplásica/genética , Metástase Neoplásica/ultraestrutura , Timosina/análise , Northern Blotting , Linhagem Celular Tumoral , Humanos , Imuno-Histoquímica , RNA Mensageiro/análiseRESUMO
OBJECTIVE: To investigate expression and significance of hTERT, telomerase associated-regulation protein (TRAP) and proliferating cell nuclear antigen (PCNA) in ovarian epithelial tumors. METHODS: 106 specimens of ovarian epithelial tumors and their clinical history were collected, including 54 cases of malignancy, 33 borderline cases and 19 benign tumor cases. Immunohistochemical staining for hTERT, TRAP and PCNA were performed. Follow-up information was obtained for 45 of 87 cases (malignancy in 54 and borderline malignancy in 33). RESULTS: The expression of hTERT was significantly different between benign (4/19) and borderline (90.9%, 30/33) cases, benign and malignant (94.4%, 51/54) cases (P < 0.001), as was the expression of TRAP between benign (4/15) and malignant (77.8%, 28/36) cases (P < 0.001). The expression of hTERT and TRAP was not higher in stage III, IV ovarian cancer patients than in stage I and II (P > 0.05, P > 0.3). The expression of PCNA between benign (6.9 +/- 5.9)% and borderline (26.4 +/- 17.8)% cases, benign and malignant (51.8 +/- 22.1)% cases, and borderline and malignant cases were different, and were statistically significant (P < 0.01, P < 0.001, P < 0.05). 33 cases of borderline malignancy are all survive. In 54 cases of malignancy, 35 of them have metastasis (64.8%), including 5 cases of lymph nodes metastasis. 4 of them died (7.4%). CONCLUSIONS: The expression of hTERT and TRAP is associated with the malignant degree of ovarian cancer, but does not correlate with stage. The expression of TRAP resembles hTERT, which may be a new tumor-associated gene. Telomerase activity is positively associated with PCNA.