RESUMO
OBJECTIVE: To observe the effects of moxibustion at "Tianshu"ï¼ST25ï¼ and "Shangjuxu"ï¼ST37ï¼ on the colonic metabolites and inflammatory factors in rats with Crohn's diseaseï¼CDï¼, so as to explore the mechanisms of moxibustion in protecting colon of CD rats based on metabolomics. METHODS: Twelve rats were first randomly selected from 36 male SD rats as a normal groupï¼NGï¼. The CD model was induced by 2, 4, 6 trinitrobenzene sulfonic acidï¼TNBSï¼ enema on the rest 24 rats. After successful modeling, rats were randomly divided into modelï¼TNBSï¼ and moxibustionï¼TNBS+MOXï¼ groupsï¼n=10 rats/groupï¼. Moxibustion was applied at bilateral ST25 and ST37 for 30 min, once daily for 7 consecutive days in the TNBS+MOX group, while rats in the NG and TNBS groups did not receive any interventions. Body weight of rats was recorded and disease activity indexï¼DAIï¼ was assessed during the experiment. After interventions, HE staining was performed to observe pathological damage of colon. Serum levels of inflammatory factors were measured by ELISA. NMR hydrogen spectroscopy was used to detect colonic metabolites of each group, and orthogonal partial least squares discriminant analysisï¼OPLS-DAï¼ was used to screen differential colonic metabolites between groups, followed by pathway analysis using MetaboAnalyst 5.0 platform. RESULTS: After modeling, compared with the NG group, the body weight of the rats in the TNBS group was significantly decreasedï¼P<0.05ï¼, the DAI score was increased ï¼P<0.05ï¼, the colon had obvious inflammatory damage and the pathological injury index was increasedï¼P<0.05ï¼, and levels of serum tumor necrosis factor-αï¼TNF-αï¼, interleukinï¼ILï¼-1ß and interferon-γï¼IFN-Î³ï¼ were significantly increasedï¼P<0.05ï¼. After moxibustion intervention, compared with the TNBS group, the body weight was significantly increasedï¼P<0.05ï¼, while the levels of serum TNF-α, IL-1ß, IFN-γ, and DAI score of the rats in the TNBS+MOX group were significantly decreasedï¼P<0.05ï¼, with alleviated colonic inflammatory injury detected by HE staining. Compared with the NG group, the relative expressions of colonic hypoxanthine, betaine, creatine, inositol, taurine, uracil, and methanol of the TNBS group were decreasedï¼P<0.05ï¼, while the relative expressions of histidine, leucine, proline, lysine, isoleucine, phenylalanine, tyrosine, propionic acid, and valine were increasedï¼P<0.05ï¼ in the TNBS group, among which, relative expressions of hypoxanthine, leucine, lysine, isoleucine, betaine, tyrosine, and taurine were reversed in the TNBS+MOX group relevant to the TNBS group, mainly involving phenylalanine, tyrosine and tryptophan biosynthesis, and taurine and subtaurine metabolism pathway. CONCLUSION: The mechanism of moxibustion at ST25 and ST37 for CD may be related to improving colon metabolic disorder state by regulating multiple metabolic metabolites and metabolic pathways, and reducing the level of inflammatory factors, so as to maintain intestinal immune homeostasis.
Assuntos
Doença de Crohn , Moxibustão , Animais , Masculino , Ratos , Betaína , Peso Corporal , Colo , Doença de Crohn/terapia , Hipoxantinas , Isoleucina , Leucina , Lisina , Ratos Sprague-Dawley , Fator de Necrose Tumoral alfa/genéticaRESUMO
OBJECTIVE: To investigate the effects of lipopolysaccharide (LPS) and heat co-exposure on serum urea and creatinine (Cr) concentrations in rats. METHODS: Male Wistar rats were randomized into normothermic saline injection control group (group C), heat exposure saline injection group (group H), normothermic LPS injection group (group L), and heat exposure LPS injection group (group HL). The rats in groups H and HL were exposed to heat in a chamber at an dry bulb temperature (Tdb) of 35.0-/+0.5 degrees Celsius, and those in groups C and L were kept in a chamber at Tdb of 26-/+0.5 degrees Celsius. LPS (8 mg/kg) was injected via the tail vein in the rats in groups L and HL to induce endotoxemia, while those in groups C and H were given normal saline injection (8 ml/kg) via the tail vein. The serum levels of urea and Cr were determined at the time points of 0, 40, 80, and 120 min after the injections. RESULTS: No significant difference was found in serum Cr level at any level of the main effects of time, drug, or Tdb (P>0.05), but serum urea level varied significantly between the different time points, different levels of Tdb, and the drug injections (P<0.01). Significant interactions were identified between the time after injection, injected agents, and Tdb (P<0.01). Except for those in the group C, all rats showed elevated serum urea levels 40 min after the injection, particularly those in group HL. The serum urea levels were positively correlated to the level of tumor necrosis factor-alpha (P<0.01). CONCLUSION: Co-exposure to LPS and heat in rats may elicit and worsen systemic inflammatory response syndrome and kidney injury.