RESUMO
The concentration of vanillymandelic acid (VMA) in urine is closely related with pheochromocytoma diagnosis. Thus, it is essential to develop more accurate and convenient fluorescence sensing strategies toward VMA. Until now, the design of double ratiometric detection methods for VMA was still in the unexplored stage. In this work, novel Ln3+-based metal-organic frameworks (QBA-Eu and QBA-Gd0.875Eu0.125) possessing dual emission peaks was fabricated successfully, which served as isomers of YNU-1 and exhibited more excellent water stability in fluorescence and structure than the ones of YNU-1. The formation of the complex between QBA ligands and VMA molecules via hydrogen bonds within QBA-Eu frameworks produced a new emission band centered at 450 nm and resulted in the decline of monomer emission intensity for QBA at 390 nm. Owing to the reduced energy gap [ΔE (S1 - T1)], the antenna effect was hampered and luminescence of Eu3+ ions also decreased. The developed double ratiometric (I615nm/I475nm, I390nm/I475nm) fluorescence sensors based on QBA-Eu and QBA-Gd0.875Eu0.125 possessed the advantages of fast response (4 min), low detection limits (0.58 and 0.51; 0.22 and 0.31 µM), and wide linear ranges (2-100 and 2-80 µM), which met the requirements of pheochromocytoma diagnosis. We also applied them to determine VMA in an artificial urine sample and diluted human urine sample and obtained satisfactory results. They will become prospective fluorescence sensing platforms for VMA.
Assuntos
Neoplasias das Glândulas Suprarrenais , Elementos da Série dos Lantanídeos , Estruturas Metalorgânicas , Feocromocitoma , Humanos , Elementos da Série dos Lantanídeos/química , Estruturas Metalorgânicas/química , Corantes Fluorescentes/química , Estudos Prospectivos , Espectrometria de Fluorescência/métodosRESUMO
Osteosarcoma (OS) is the most frequent type of cancer that starts in the bones, with a rather high tendency to metastasize to other bones at the early stages. Although many types of research have demonstrated that long noncoding RNAs commonly take part in the development of various cancers, the modulating mechanism of LEF1-AS1 in OS was unknown yet. In this study, our results disclosed that LEF1-AS1, as well as LEF1, had higher expression levels in OS cells than that in normal bone cells. LEF1-AS1 knockdown dramatically inhibited the proliferation, migration, as well as invasion in OS, which proved that LEF1-AS1 contributed to the growth of OS. Furthermore, HNRNPL knockdown suppressed the expression of LEF1. LEF1-AS1 was confirmed to sponge HNRNPL and HNRNPL could bind with LEF1. Both LEF1-AS1 and HNRNPL could enhance the stability of LEF1 mRNA. LEF1-AS1 acted as a promoter in stimulating the Wnt signaling pathway in OS. In rescue experiments, overexpression of LEF1 partially offset the inhibition LEF1-AS1 knockdown brought in the proliferation, migration as well as invasion of OS cells. Collectively, this study had investigated that LEF1-AS1 bound with HNRNPL to promote OS cell proliferation, migration as well as invasion by enhancing the messenger RNA stability of LEF1.
Assuntos
Movimento Celular/genética , Proliferação de Células/genética , Fator 1 de Ligação ao Facilitador Linfoide/genética , Osteossarcoma/metabolismo , Osteossarcoma/patologia , Estabilidade de RNA/genética , RNA Longo não Codificante/metabolismo , Ribonucleoproteínas/metabolismo , Via de Sinalização Wnt/genética , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/genética , Técnicas de Silenciamento de Genes , Humanos , Camundongos , Camundongos Nus , Invasividade Neoplásica/genética , RNA Longo não Codificante/genética , RNA Mensageiro/química , Ribonucleoproteínas/genética , Transfecção , Carga Tumoral/genética , Ensaios Antitumorais Modelo de XenoenxertoRESUMO
Bone homeostasis is known as a dynamic balance, including bone formation through osteoblasts and bone resorption by osteoclasts. MicroRNAs (miRs) play a critical role in regulating bone formation and homeostasis. In the study, the effects of miR-451a on bone homeostasis were investigated. The results indicated that the primary osteoblasts and mesenchymal stem cells (MSCs), as the main source of osteoblasts, isolated from miR-451a-knockout (KO) mice showed promoted osteogenesis. in vivo, an ovariectomized (OVX) animal model was used to further explore the effect of miR-451a on osteoporosis. Micro-computed tomography (µCT) indicated a promoted bone volume in miR-451a-KO mice compared to wild-type (WT) mice after OVX operation, demonstrating a redundant bone formation after the knockout of miR-451a. Importantly, we for the first time found that bone morphogenetic protein 6 (Bmp6) was a direct target of miR-451a, elevating bone formation through regulating SMAD1/5/8 expression. In conclusion, reducing miR-451a expression levels could enhance bone formation during the progression of osteoporosis, which might be at least partly via the meditation of Bmp6 expression.
Assuntos
Proteína Morfogenética Óssea 6/metabolismo , Reabsorção Óssea/metabolismo , Células-Tronco Mesenquimais/metabolismo , MicroRNAs/metabolismo , Osteoblastos/metabolismo , Osteogênese , Osteoporose Pós-Menopausa/metabolismo , Animais , Proteína Morfogenética Óssea 6/genética , Reabsorção Óssea/genética , Reabsorção Óssea/patologia , Células Cultivadas , Modelos Animais de Doenças , Regulação para Baixo , Feminino , Humanos , Células-Tronco Mesenquimais/patologia , Camundongos Knockout , MicroRNAs/genética , Osteoblastos/patologia , Osteoporose Pós-Menopausa/genética , Osteoporose Pós-Menopausa/patologia , Ovariectomia , Transdução de Sinais , Proteína Smad1/metabolismo , Proteína Smad5/metabolismo , Proteína Smad8/metabolismoRESUMO
A series of Ag-K/MnO2 nanorods with various molar ratios of K/Ag were synthesized by a conventional wetness incipient impregnation method. The as-prepared catalysts were used for the catalytic oxidation of HCHO. The Ag-K/MnO2 nanorods with an optimal K/Ag molar ratio of 0.9 demonstrated excellent HCHO conversion efficiency of 100% at a low temperature of 60 °C. The structures of the samples were investigated by BET, TEM, SEM, XRD, H2-TPR, O2-TPD and XPS. The results showed that Ag-0.9K/MnO2-r exhibited more facile reducibility and greatly abundant surface active oxygen species, endowing it with the best catalytic activity of the studied catalysts. This work provides new insights into the development of low-cost and highly efficient catalysts for the removal of HCHO.