RESUMO
BACKGROUND: Vascular calcification during aging is highly prevalent in patients with cardiovascular disease; however, there is still no improvement in clarifying the development of vascular calcification. FOSL1 is a transcription regulator belonging to the AP-1 family, which has a unique function in vascular senescence, but its role in vascular calcification needs to be further explored. METHODS: Primary mouse vascular smooth muscle cells were isolated and used to construct a calcification model in vitro. Seven-week-old male C57BL/6 mice were used to build the vitD3-induced calcification model in vivo. qRT-PCR and western blot were used to verify the expression of FOSL1 and other genes expressed in vascular smooth muscle cells and aortas. The level of calcification was determined by Alizarin Red S (ARS) staining and the calcium content assay. The level of cellular GSH was detected by the GSH assay kit. RESULTS: Here, we report that FOSL1 was up-regulated after high-calcium/phosphate treatment in both the in vivo and in vitro vascular calcification models. Functional studies have shown that the reduction of FOSL1 attenuates ferroptosis and calcification in vascular smooth muscle cells, as indicated by ARS staining, calcium content assay, and western blot. The inhibition of FOSL1 downregulated the expression of bone-related molecules including Msh Homeobox 2 (MSX2) and tumor necrosis factor receptor superfamily, member 11b/osteoprotegerin (OPG), suggesting that FOSL1 promoted osteogenic differentiation of vascular smooth muscle cells. Furthermore, we found that the ferroptosis-inducing drug erastin can significantly accelerate calcification in the aortic ring while Ferrostatin-1 (fer-1), a drug to protect cells from ferroptosis, can alleviate calcification. Further experiments have shown that inhibiting FOSL1 can promote the expression of ferroptosis-related genes and attenuate calcification. Functionally, cellular GSH levels were increased after the reduction of FOSL1. CONCLUSIONS: In this study, we observed a significant protective effect when we reduced the expression of FOSL1 during vascular calcification, and this effect might regulate ferroptosis to a great extent.
RESUMO
Countries all over the world are looking for fuel to replace fossil energy due to environmental concerns and a scarcity of fossil fuels. Oil shale (OS) and rice husk (RH) are both viable fuels, although they both have issues like high ash content and poor calorific value. OS and RH were used as feedstock for high-quality fuel in this study, which uses a hydrothermal technique to provide a novel way to utilize OS and rice. At different hydrothermal temperatures (150, 200 and 250 °C), including combustion and pyrolysis processes, the thermogravimetric analyzer (TGA) was used to analyse thermal transformation characteristics of co-hydrothermal carbonization (co-HTC) of OS and RH, as well as the synergistic effects. Results showed that the co-HTC pretreatment had a significant effect on the thermal transformation behaviour of OS and RH. On the one hand, the co-HTC has higher volatile content than its calculated value. On the other hand, a synergistic effect was found in combustion processes, and this effect was the most obvious when the hydrothermal temperature was around 200 °C, and the characteristic peak of functional groups vibration was strong. Therefore, the co-HTC was considered suitable for combustion. The combination of co-HTC modification with subsequent thermochemical processes has positive implications for the energy production and utilization of organic waste.