RESUMO
Anticoagulant-induced aggregation of platelets leads to pseudothrombocytopenia. Blood cell counters generally trigger alarms to alert the user. We describe an insidious case of pseudothrombocytopenia, where the complete absence of Coulter counter alarms both in ethylenediaminetetraacetic acid blood and in citrate or acid citrate dextrose blood samples was compounded by the fact that the massive aggregates were exclusively found at the edges of the blood smear. Non-recognition of pseudothrombocytopenia can have serious diagnostic and therapeutic consequences. While the anti-aggregant mixture citrate-theophylline-adenosine-dipyridamole completely failed in preventing pseudothrombocytopenia, addition of iloprost to anticoagulants only partially prevented the aggregation. Only the prior addition of gentamicin to any anticoagulant used resulted in a complete prevention of pseudothrombocytopenia and enabled to count accurately the platelets.
Assuntos
Anticoagulantes/efeitos adversos , Contagem de Plaquetas , Trombocitopenia/sangue , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Iloprosta/administração & dosagem , Agregação Plaquetária/efeitos dos fármacosRESUMO
The well-known Kjeldahl method for the determination of faecal nitrogen is rather complex, time-consuming and expensive. Therefore, the use of near-infrared spectroscopy in determining the amount of nitrogen in faeces has been studied. To our knowledge we are the first to present the calibration equation for the determination of nitrogen with near-infrared spectroscopy. A good correlation (r = 0.96) was found between results from near-infrared spectroscopy and the Kjeldahl method. The imprecision of both methods was comparable. Once the rather laborious calibration has been performed, near-infrared spectroscopy is shown to be a very simple and rapid method for measuring nitrogen in faeces.
Assuntos
Fezes/química , Nitrogênio/análise , Adulto , Calibragem , Fibrose Cística/complicações , Fibrose Cística/metabolismo , Humanos , Síndromes de Malabsorção/complicações , Espectrofotometria InfravermelhoRESUMO
The applicability of near-infrared spectroscopy to determine the amount of fat in faeces has been investigated. Near-infrared spectroscopy was favourably compared with the well known titrimetric method (Van de Kamer et al., J Biol Chem 1948; 177:347-55). A good correlation between near-infrared spectroscopy and the titrimetric method was found. The measurement of faecal fat by near-infrared spectroscopy is found to be more precise than the manual method. Moreover, near-infrared spectroscopy is shown to be a very simple and rapid method for measuring fat in faeces. However, it was shown that performing one's own calibration curve is necessary. Due to this necessity and the costs of the apparatus application of near-infrared spectroscopy is especially advantageous in laboratories with a substantial amount of samples to be analysed.