RESUMO
Incidence of white matter injury (WMI), which is featured as softening of white matter tissues, has recently increased. Previous studies have demonstrated a close correlation between T helper cell 1 and T helper cell 2 (Th1/Th2) imbalance and nuclear factorκB (NFκB) with brain disease. Their role in premature WMI, however, remains to be illustrated. Serum samples were collected from 60 premature WMI neonates, plus another control group of 60 premature babies without WMI. Patients were further divided into mild, moderate and severe WMI groups. Reverse transcription quantitative polymerase chain reaction was used to test mRNA expression levels of Th1/Th2 cytokines, including interleukin 2 (IL)2, tumor necrosis factorα (TNFα), IL4, IL10 and nuclear factor (NF)κB, whilst their serum levels were measured by ELISA. Their correlation with disease occurrence and progression were further analysed, to illustrate the effect of Th1/Th2 balance and NFκB on pathology of premature WMI. Serum levels of IL4 and IL10 were significantly decreased in premature WMI babies, whilst IL2, TNFα and NFκB were upregulated (P<0.05 vs. control group). With aggravated disease, IL4 and IL10 expression was further decreased while IL2, TNFα and NFκB were increased (P<0.05 vs. mild WMI group). Th1 cytokines IL2 and TNFα and NFκB were negatively correlated with Th2 cytokines IL4 and IL10. Disease severity was positively correlated with IL2, TNFα and NFκB expression, and was negatively correlated with IL4 and IL10 (P<0.05). Th1/Th2 imbalance and NFκB upregulation were observed in WMI pathogenesis, with elevated secretion of Th1 cytokines and decreased Th2 cytokines, suggesting that Th1/Th2 imbalance and NFκB upregulation may be a potential indicator for the early diagnosis and treatment of WMI pathogenesis and progression.