Vaccination of African penguins (Spheniscus demersus) against high-pathogenicity avian influenza.

BACKGROUND: High-pathogenicity avian influenza (HPAI) has become a conservation threat to wild birds. Therefore, suitable vaccine technology and practical application methods require investigation. METHODS: Twenty-four African penguins (Spheniscus demersus) were vaccinated with either a conventional inactivated clade 2.3.4.4b H5N8 HPAI whole virus or a tobacco leaf-produced H5 haemagglutinin-based virus-like particle (VLP). Six birds received a second dose of the inactivated vaccine. Antibody responses were assessed and compared by employing haemagglutination inhibition tests. RESULTS: A second dose of inactivated vaccine was required to induce antibody titres above the level required to suppress virus shedding, while a single dose of VLP vaccine produced these levels by day 14, and one bird still had antibodies on day 430. LIMITATIONS: Bacterial contamination of the VLP vaccine limited the monitoring period and sample size in that treatment group, and it was not possible to perform a challenge study with field virus. CONCLUSION: VLP vaccines offer a more practical option than inactivated whole viruses, especially in logistically challenging situations involving wild birds.

Factors determining the number of seabirds impacted by oil spills and the success of their rehabilitation: Lessons learned from Namibia and South Africa.

The coastal waters of Namibia and South Africa have an extensive history of oil spills, with 71 recorded up to 2021. Thirty-nine spills reportedly affected 83,224 seabirds, with African penguins (Spheniscus demersus; 91.0 %) and Cape gannets (Morus capensis; 8.5 %) most affected. Spills affecting seabirds were caused by unknown sources (46 %), bulk/cargo carriers (43 %), tankers (38 %) and ship-to-ship transfers (14 %). The number of penguins oiled was predicted by the breeding population size within 25 to 75 km, but not the volume of oiled spilled, the month or the year. Rehabilitation records from penguins oiled in spills since 2001 reveal that the day of admission (relative to the start of the spill) was predictive of packed cell volume, body mass, and plasma total solids, with the latter two being predictive of rehabilitation success. Our results highlight the importance of rapid monitoring at colonies to locate oiled birds in the event of spills.