RESUMO
The serological response towards Staphylococcus saprophyticus in young women with symptomatic urinary tract infection was measured with an enzyme-linked immunosorbent assay (ELISA) and a direct agglutination (DA) test. In both assays a marked antibody response was found in 2 of 3 patients with acute pyelonephritis caused by S. saprophyticus. Patients with acute cystitis due to S. saprophyticus or Escherichia coli showed antibody titers comparable to those of healthy controls.
Assuntos
Anticorpos Antibacterianos/biossíntese , Infecções Estafilocócicas/imunologia , Staphylococcus/imunologia , Infecções Urinárias/imunologia , Doença Aguda , Adolescente , Adulto , Testes de Aglutinação , Proteínas de Bactérias/análise , Proteínas de Bactérias/imunologia , Cistite/imunologia , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Infecções por Escherichia coli/imunologia , Feminino , Humanos , Pessoa de Meia-Idade , Pielonefrite/imunologiaRESUMO
Extracellular products from Staphylococcus saprophyticus were isolated and tested for their effect on the lymphoproliferative activity of con A and LPS and on the growth of human lymphocytes. The stimulatory effects of con A or LPS were not affected but a T-cell mitogenic activity was noted after 5 days of incubation. The extracellular material was separated by gel chromatography and the mitogenic activity was shown to be located to the protein fraction.