RESUMO
Due to the bioavailability and movement of antimony in trophic web, the overexploitation of antimony mine resulted in antimony contamination that harmed the ecology nearby, raising concerns for public health. Whereas, most researches focused on the removal of antimony in the aqueous instead of the immobilization of antimony in the soil. Herein, the immobilized performance of biochar (BC) loaded with nano zero-valent iron (nZVI-BC) on antimony in the soil near the smelting area was researched through pot experiments for the first time, and its stabilization mechanism on antimony was investigated by valent state variation of antimony. The results demonstrated that BC restricted the cation exchange capacity and catalase activity in the soil, while nZVI-BC had a favorable and negative impact on two variables, respectively. The nZVI-BC showed more stable immobilization capacity on antimony over time than BC, whose exchangeable speciation only marginally rose (2%-6%), although the exchangeable speciation of antimony fell both from 15% to 2% after adding the BC and nZVI-BC, The electron attraction force between nZVI-BC and antimony was also intensified owing to the oxidation-reduction process, which was considered as the stabilizing principle of nZVI-BC on antimony in soil. Furthermore, the decreased bioaccumulation factor for the perennial ryegrass (0.46-0.21) and Galinsoga parviflora Cav. (0.26-0.17) stated that the BC effectively mitigated the bioaccumulation risk of antimony.
Assuntos
Antimônio , Carvão Vegetal , Recuperação e Remediação Ambiental , Ferro , Mineração , Poluentes do Solo , Solo , Antimônio/química , Carvão Vegetal/química , Poluentes do Solo/química , Poluentes do Solo/análise , Ferro/química , Recuperação e Remediação Ambiental/métodos , Solo/química , Disponibilidade BiológicaRESUMO
BACKGROUND: This study aims to investigate the effects of a conditioned medium (CM) from human umbilical cord mesenchymal stem cells (HuMSCs) cultivated in gelatin sponge (GS-HuMSCs-CM) on hair growth in a mouse model. METHODS: CM was collected from the HuMSCs cultivated in a monolayer or in a gelatin sponge. Vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), keratinocyte growth factor (KGF), and hepatocyte growth factor (HGF) levels in CMs were measured by enzyme-linked immunosorbent assays (ELISAs). A hair loss model by a C57 BL/6J mouse was prepared. The effects of GS-HuMSCs-CM and HuMSCs on hair regrowth in mice were investigated by intradermal injection in the depilated back skin with normal saline (NS) as the control. The time for hair regrowth and full covering in depilated areas was observed, and the hair growth was evaluated histologically and by grossly measuring hair length and diameter. RESULTS: Compared with monolayer cultured cells, the three-dimensional (3D) culture of HuMSCs in gelatin sponge drastically increased VEGF, IGF-1, KGF, and HGF production. GS-HuMSCs-CM and HuMSCs injection both promoted hair regeneration in mice, while GS-HuMSCs-CM presented more enhanced effects in hair length, hair diameter, and growth rate. GS-HuMSCs-CM significantly promoted angiogenesis in injected skin areas, which might also contribute to faster hair regrowth. CONCLUSION: GS-HuMSCs-CM exerted significant effects on inducing hair growth and promoted skin angiogenesis in C57BL/6J mice.
Assuntos
Cabelo , Fator de Crescimento Insulin-Like I , Células-Tronco Mesenquimais , Cordão Umbilical , Animais , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , Humanos , Meios de Cultivo Condicionados/farmacologia , Camundongos , Cordão Umbilical/citologia , Cabelo/crescimento & desenvolvimento , Cabelo/efeitos dos fármacos , Fator de Crescimento Insulin-Like I/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Fator de Crescimento de Hepatócito/metabolismo , Gelatina/química , Alicerces Teciduais/química , Camundongos Endogâmicos C57BL , Células Cultivadas , Fator 7 de Crescimento de Fibroblastos/metabolismoRESUMO
The discharge of tailing leachate and metallurgical wastewater has led to an increasing trend of water pollution. In this study, nZVI-modified low-temperature biochar was used to adsorb Sb(III) from water. The adsorption capacity and speed of nZVI-BC were better than those of BC, and the best adsorption effect was observed for 4nZVI-BC, with 93.60 mg·g-1 maximum adsorptive capacity, which was 208.61% higher than the original BC. The Langmuir and Temkin models were well fitted (R2 ≥ 0.99), and PSO was more in line with the 4nZVI-BC adsorption process, indicating that the adsorption was a monolayer physico-chemical adsorption. The combination of XRD, FTIR, and XPS characterization demonstrated that the adsorption mechanism predominantly included redox reactions, complexation, and electrostatic interactions. The thermodynamic results demonstrated that 4nZVI-BC adsorption on Sb(III) was a spontaneous endothermic process. Additionally, the order of the influence of interfering ions on 4nZVI-BC was CO32- > H2PO4- > SO42- > Cl-. After three repeated uses and adsorption-desorption, the adsorption ratio of Sb(III) by 4nZVI-BC was still as high as 90% and 65%, respectively. This study provides a theoretical reference for the exploration and development of Sb(III) removal technologies for aquatic environments.
Assuntos
Ferro , Poluentes Químicos da Água , Ferro/química , Antimônio , Adsorção , Poluentes Químicos da Água/análise , Carvão Vegetal/químicaRESUMO
BACKGROUND: Axenfeld-Rieger syndrome (ARS) is a rare kind of anterior segment dysgenesis (ASD). The most common ocular features of ARS are posterior embryotoxon and iris hypoplasia, while some patients may manifest as corneal opacity and edema. However, the current understanding of how ARS affects the cornea is still incomplete. This study reports a novel histopathological finding of ARS, complicating corneal abnormalities, including congenital corneal opacity and irreversible endothelial decompensation. METHODS: This retrospective study included 6 eyes of 3 ARS patients, 5 of which underwent keratoplasty for irreversible endothelial decompensation from May 2016 to January 2019. No eye had a history of surgery. We reviewed the data of epidemiology, clinical manifestations and histopathologic examinations. RESULTS: Five eyes developed irreversible endothelial decompensation, among which 4 were born with corneal opacity. One eye exhibited transparent cornea but showed a continuous loss of endothelial cells in the absence of surgery and elevated intraocular pressure thereafter. Anterior segment optical coherence tomography photographs showed that anterior synechia existed in the area with corneal opacities, where we found the interlayer splitting of the Descemet membrane inserted by hypoplastic iris and a basement membrane-like structure under a light microscope. CONCLUSION: Anterior synechia might be associated with corneal abnormalities in ARS patients. The novel histopathologic finding revealed the internal relation between anterior segment dysgenesis and would help explore the inner mechanism of corneal abnormalities in ARS.
Assuntos
Doenças da Córnea , Opacidade da Córnea , Anormalidades do Olho , Doenças da Íris , Humanos , Células Endoteliais/patologia , Estudos Retrospectivos , Segmento Anterior do Olho/anormalidades , Anormalidades do Olho/patologia , Doenças da Córnea/patologia , Opacidade da Córnea/complicações , Doenças da Íris/patologiaRESUMO
Hydroxyproline-O-galactosyltransferase (GALT) initiates O-glycosylation of arabinogalactan-proteins (AGPs). We previously characterized GALT2 (At4g21060), and now report on functional characterization of GALT5 (At1g74800). GALT5 was identified using heterologous expression in Pichia and an in vitro GALT assay. Product characterization showed GALT5 specifically adds galactose to hydroxyproline in AGP protein backbones. Functions of GALT2 and GALT5 were elucidated by phenotypic analysis of single and double mutant plants. Allelic galt5 and galt2 mutants, and particularly galt2 galt5 double mutants, demonstrated lower GALT activities and reductions in ß-Yariv-precipitated AGPs compared to wild type. Mutant plants showed pleiotropic growth and development phenotypes (defects in root hair growth, root elongation, pollen tube growth, flowering time, leaf development, silique length, and inflorescence growth), which were most severe in the double mutants. Conditional mutant phenotypes were also observed, including salt-hypersensitive root growth and root tip swelling as well as reduced inhibition of pollen tube growth and root growth in response to ß-Yariv reagent. These mutants also phenocopy mutants for an AGP, SOS5, and two cell wall receptor-like kinases, FEI1 and FEI2, which exist in a genetic signaling pathway. In summary, GALT5 and GALT2 function as redundant GALTs that control AGP O-glycosylation, which is essential for normal growth and development.