M2 macrophage-polarized anti-inflammatory microneedle patch for accelerating biofilm-infected diabetic wound healing via modulating the insulin pathway.

Macrophages play a pivotal role in the healing of diabetic ulcers. The sustained elevation of glucose levels damages the insulin signaling pathway in macrophages, leading to dysfunctional macrophages that struggle to transition from pro-inflammatory (M1) to reparative (M2) states. Therefore, modulating macrophage inflammatory responses via the insulin pathway holds promise for diabetic ulcer treatment. Additionally, the presence of biofilm impedes drug penetration, and the resulting immunosuppressive microenvironment exacerbates the persistent infiltration of pro-inflammatory M1 macrophages. Therefore, we designed an array of dissolvable microneedle (denoted as NPF@MN) loaded with self-assembled nanoparticles that could deliver NPF nanoparticles, acid-sensitive NPF-releasing Protocatechualdehyde (PA) with hypoglycemic and insulin-like effects, regulating macrophage polarization to an anti-inflammatory M2 phenotype. Additionally, this study extensively examined the mechanism by which NPF@MN accelerates the healing of diabetic ulcers through the activation of the insulin signaling pathway. Through RNA-seq and GSEA analysis, we identified a reduction in the expression of pathway-related factors such as IR, IRS-1, IRS-2, and SHC. Our work presents an innovative therapeutic approach targeting the insulin pathway in diabetic ulcers and underscores its translational potential for clinical management.

Long-term outcomes of IVUS-guided and angiography-guided drug-eluting stent implantation for left main coronary artery disease: a retrospective consort study.

BACKGROUND: In patients with unprotected left main coronary artery disease (ULMCAD), this study compared the long-term prognosis of drug-eluting stent insertion guided by intravascular ultrasonography (IVUS) vs. angiography. PATIENTS AND METHODS: This retrospective consort investigation was performed in December 2021. This analysis included 199 patients who underwent IVUS-guided (IVUS group, n = 81) or angiography-guided (angiography group, n = 118) drug-eluting stent implantation at the Affiliated Hospital of Inner Mongolia Medical University between September 2013 and September 2018. Major adverse cardiac events (MACE) were defined as cardiovascular death, sudden cardiac death, myocardial infarction. RESULTS: The IVUS group had considerably lower proportions of MACE within 1 year postoperatively (P = 0.002) and cardiac mortality within 3 years postoperatively (P = 0.018) compared to the angiography group. However, after adjusting for confounding variables, the hazard ratio for 3-year cardiac mortality was similar between the two groups (P = 0.28). In the IVUS group, there was considerably greater minimum lumen diameter (MLD) (P = 0.046), and reduced frequencies of target vessel restenosis (P < 0.050) and myocardial infarction (MI) (P = 0.024) compared to the angiography group. Cox regression analysis for 3-year cardiac mortality found that MSD was independently associated with low cardiac mortality (HR = 0.1, 95% CI: 0.01-14.92, P = 0.030). CONCLUSION: IVUS-guided drug-eluting stent implantation may lead to better long-term prognosis in patients with ULMCAD, and MSD may be a predictor for lower cardiac mortality.

Interactions between Helicobacter pylori infection and host metabolic homeostasis: A comprehensive review.

The microbiota actively and extensively participates in the regulation of human metabolism, playing a crucial role in the development of metabolic diseases. Helicobacter pylori (H. pylori), when colonizing gastric epithelial cells, not only induces local tissue inflammation or malignant transformation but also leads to systemic and partial changes in host metabolism. These shifts can be mediated through direct contact, toxic components, or indirect immune responses. Consequently, they influence various molecular metabolic events that impact nutritional status and iron absorption in the host. Unraveling the intricate and diverse molecular interaction links between H. pylori and human metabolism modulation is essential for understanding pathogenesis mechanisms and developing targeted treatments for related diseases. However, significant challenges persist in comprehensively understanding the complex association networks among H. pylori itself, the infected host's status, the host microbiome, and the immune response. Previous metabolomics research has indicated that H. pylori infection and eradication may selectively shape the metabolite and microbial profiles of gastric lesions. Yet, it remains largely unknown how these diverse metabolic pathways, including isovaleric acid, cholesterol, fatty acids, and phospholipids, specifically modulate gastric carcinogenesis or affect the host's serum metabolism, consequently leading to the development of metabolic-associated diseases. The direct contribution of H. pylori to metabolisms still lacks conclusive evidence. In this review, we summarize recent advances in clinical evidence highlighting associations between chronic H. pylori infection and metabolic diseases, as well as its potential molecular regulatory patterns.

Antioxidant-Engineered Milk-Derived Extracellular Vesicles for Accelerating Wound Healing via Regulation of the PI3K-AKT Signaling Pathway.

Inspired by the experience of relieving inflammation in infants with milk, antioxidant-engineered milk-derived extracellular vesicles (MEVs) are developed to evaluate their potential for accelerating wound healing. In this work, MEVs with polydopamines (PDA) are engineered using the co-extrusion method. Subsequently, the authors incorporated them into a Schiff-based crosslink hydrogel, forming a skin dosage form that could facilitate the wound healing process. The antioxidant properties of PDA assist in the anti-inflammatory function of engineered MEVs, while the gel provides better skin residency. The PDA@MEVs+GEL formulation exhibits excellent biocompatibility, pro-angiogenic capacity, and antioxidant ability in vitro. Furthermore, in vivo experiments demonstrate its efficacy in wound repair and inflammation inhibition. Mechanistically, PDA@MEVs+GEL simultaneously promotes the growth, migration, and anti-inflammation of 3T3 cells by activating PI3K-AKT pathway. Moreover, PDA@MEVs+GEL exhibits enhanced functionality in promoting wound healing in vivo, attributed to its ability to inhibit inflammation, stimulate angiogenesis, and promote collagen synthesis. In conclusion, this study delves into the mechanism of MEVs and underscores the improved efficacy of engineered extracellular vesicles. Additionally, the feasibility and prospect of engineered MEVs in treating skin wounds are verified, suggesting that antioxidant-engineered MEVs could be a promising therapeutic agent for wound healing applications.

Effects of NSC in different organs and at different growth stages on the yield of oil peony Fengdan with different ages.

Non-structural carbohydrates (NSC) as resource reserves of plants play important roles in energy supply for normal growth and reproduction under environmental stress. The yield of perennial crops is mainly determined by the carbohydrate production and allocation in the current growth season, as well as the re-allocation of NSC reserves. However, the contribution of NSC to crop yield has not been fully determined. Fengdan (Paeonia ostii) is a variety of oil Peony that is newly developed in China. The effects of tree age and NSC on yield were examined by investigated the variations of biomass, soluble sugars, starch, and NSC in the organ and whole tree levels in the dormant and ripening stages of Fengdan populations with 4-, 6-, and 8-year-old in 2020 and 5-, 7-, and 9- year old in 2021. Results showed that the biomass, yield (seed biomass), soluble sugars, starch, and NSC reserve of Fengdan at the whole tree level increased with the increase in age. Although consistent correlations were observed between soluble sugars, starch and NSC storage, and yield among the plants with different ages, Fengdan showed allometric growth relationships between the accumulation of soluble sugars, starch, and NSC and yield and biomass (standardized major axis analyses slope b ≠ 1). Tree age significantly affected biomass and its allocation and NSC levels, especially the yield of Fengdan plants. The results of the investigation of the variations in the relationships between the yield and seasonal fluctuations of NSC and biomass indicate that roots is the key storage structure, whereas stems both serve as sink and/or source functions for the adult (7-9a) plants. NSC level, particularly the concentration of soluble sugars, in stems mainly influences Fengdan yield. These findings together provide new insights into the mechanisms underlying the yield formation of Fengdan and have implications for manipulating sink-source relationship to achieve high yield.

Dynamic tagging to drive arginine nano-assembly to metabolically potentiate immune checkpoint blockade therapy.

L-arginine metabolism is essential for the activation, survival, and effector function of the T lymphocytes and critical in eliminating tumors via T-cell-mediated immunotherapy, such as immune checkpoint blockade (ICB). Unfortunately, efficient delivery of hydrophilic L-arginine to the tumor microenvironment (TME) has met tremendous difficulties because of the limited loading efficacy and rapid diffusion. Inspired by the small-molecule prodrug nanoassemblies with ultrahigh drug-loading, we screen out aromatic aldehydes compounds to be used as dynamic tags to decorate L-arginine (reversible imine). Nano-Arginine (ArgNP, 104 nm) was created based on dynamic tag-mediated self-assembly. Molecular dynamics simulations indicate that the driving force of this self-assembly process is intermolecular hydrogen bonds, π-π stacking, and cation-π interactions. Notably, ArgNP metabolic synergy with anti-PD-L1 antibody (aPDL1) can promote tumor-infiltrating T cells (3.3-fold than aPDL1), resulting in a tumor inhibition ratio of 2.6-fold than aPDL1. Besides, such a strategy efficiently reduces the myeloid-derived suppressor cells, increases the M1-macrophages against the tumor, and induces the production of memory T cells. Furthermore, this synergistic therapy effectively restrains lung metastasis and prolongs mouse survival (60% survival ratio). The study highlights the dynamic tags strategy with facility and advance to deliver L-arginine that can metabolically promote ICB therapy.

Acidic and hypoxic tumor microenvironment regulation by CaO2-loaded polydopamine nanoparticles.

Hypoxia and high accumulation of lactic acid in the tumor microenvironment provide fertile soil for tumor development, maintenance and metastasis. Herein, we developed a calcium peroxide (CaO2)-loaded nanostructure that can play a role of "one stone kill two birds", i.e., acidic and hypoxic tumor microenvironment can be simultaneously regulated by CaO2 loaded nanostructure. Specifically, CaO2-loaded mesoporous polydopamine nanoparticles modified with sodium hyaluronate (denoted as CaO2@mPDA-SH) can gradually accumulate in a tumor site. CaO2 exposed in acidic microenvironment can succeed in consuming the lactic acid with oxygen generation simultaneously, which could remodel the acid and hypoxia tumor microenvironment. More importantly, the relief of hypoxia could further reduce lactate production from the source by down-regulating the hypoxia inducible factor-1α (HIF-1α), which further down-regulated the glycolysis associated enzymes including glycolysis-related glucose transporter 1 (GLUT1) and lactate dehydrogenase A (LDHA). As a result, CaO2@mPDA-SH alone without the employment of other therapeutics can dually regulate the tumor hypoxia and lactic acid metabolism, which efficiently represses tumor progression in promoting immune activation, antitumor metastasis, and anti-angiogenesis.

[Effects of attapulgite addition on soil evaporation and crack characteristics]. / å‡¹å‡¸æ£’åœŸæ·»åŠ å¯¹åœŸå£¤è’¸å‘åŠè£‚ç¼ç‰¹å¾çš„å½±å“.

Water is one of the most important factors limiting vegetation recovery and agricultural development in arid and semi-arid areas. The reduction of ineffective soil evaporation can improve soil water use efficiency. As a kind of clay mineral, attapulgite (ATP) plays a critical role in limiting soil evaporation due to its hydrophilicity and adsorption. In this study, three typical soils with different textures (dark loessial soil, cultivated loess soil, and sandy soil) were selected from the arid and semi-arid area of the Loess Plateau, and five ATP additions (0%, 1%, 2%, 3% and 4%) were set for conducting soil evaporation experiments under natural conditions using micro-evaporators to investigate the effects of ATP addition on different soil evaporation processes and the characteristics of evaporation surface cracks. The results showed that the cumulative evaporation and evaporation loss ratio of the same soil decreased with the increases of ATP addition when the ATP addition was <3%. When ATP was added at 3%, the cumulative evaporation and evaporation loss ratio of dark loessial soil and sandy soil decreased, while those of cultivated loess soil increased. When ATP was added at 4%, the cumulative evaporation decreased and the evaporation loss ratio increased for dark loessial soil, the cumulative evaporation increased and the evaporation loss ratio decreased for sandy soil, and the cumulative evaporation and evaporation loss ratio decreased for cultivated loess soil. The average cumulative evaporation of different soils followed an order of dark loessial soil > cultivated loess soil > sandy soil. Soil water content of ATP treatment was consistently higher than that of control throughout the whole evaporation process in the same soil. Simulations of cumulative evaporation versus the square root of time indicated that the amount of water released from the ATP-treated soil samples at the end of evaporation was higher than that of the control. After the addition of ATP, the crack area density of dark loessial soil and cultivated loess soil increased significantly, and the crack area density of sandy soil increased with the increase of ATP addition. The crack area density of all three soils reached the maximum at 4% of ATP addition. In summary, ATP addition of 3% could minimize the ineffective evaporation of soil water.

DNA-MnO2 Nanoconjugates Investigation and Application for Electrochemical Polymerase Chain Reaction.

Manganese dioxide (MnO2) nanosheets are emerging for biomedical applications with excellent physical and chemical properties. Adsorption of DNA on MnO2 is important for biosensing, bioimaging, and therapy. Nevertheless, current fundamental understanding about the interaction is preliminary. Herein, UV-vis absorption spectra are applied to systematically explore the biointerfacial interaction between DNA and MnO2 with the factors of salt concentration, pH value, temperature, DNA concentration, and length. The results offer important fundamental insights into the investigation of DNA-MnO2 nanocomposites. Meanwhile, the optimal parameters are applied to construct a screen-printed electrode (SPE) modified with polymerase chain reaction (PCR) primer-decorated MnO2 nanosheets. An electrochemical PCR system is then developed for ultrasensitive detection of circulating tumor DNA (ctDNA). The limit of detection is determined to be 0.1 fM, and high selectivity is demonstrated. Combining the merits of SPE, DNA-MnO2 nanosheets, and an amplified reaction, this developed strategy shows great promise in bioanalysis, clinical disease diagnosis, and biomedicine applications.

Application of magnetic susceptibility and heavy metal bioaccessibility to assessments of urban sandstorm contamination and health risks: Case studies from Dunhuang and Lanzhou, Northwest China.

Direct ingestion of sandstorm particles is an important pathway in human exposure to heavy metals. This study investigated the potential health risks of heavy metals transported in sandstorms from Dunhuang to Lanzhou in northwestern China using environmental magnetic parameters and metal bioaccessibilities in simulated gastric and intestinal tracts. The mean magnetic susceptibility of sandstorms in Lanzhou was 366.86 × 10-8 m3/kg, which was more than 5-fold higher than that of sandstorms in Dunhuang, indicating that these sandstorms continuously receive heavy metals with high magnetic mineral content along their pathways. Heavy metal concentrations in sandstorms were higher than background values and those in urban topsoil. Enrichment factors and pollution load indices showed that these heavy metals were derived from both natural and anthropogenic sources, with Cu, Zn, Pb, and Cd being strongly influenced by anthropogenic sources. The bioaccessibilities of Cd, Cu, Zn, and Pb in the sandstorms of Lanzhou were very high, ranging from 22.69% (Cu) to 50.86% (Pb) for gastric phase, and 12.07% (Pb)-22.11% (Cd) for interstinal phase, with the significant reduction in χlf of the physiologically-based extraction testing (PBET) treated sandstorms. The magnetic minerals are significant correlation with the concentrations of heavy metals in sandstorm and effect the release of heavy metals during human digestion process. The overall ecological risk posed by heavy metals contained in sandstorms was relatively low; however, the risk was moderate to high at individual sites. Ingestion posed the highest carcinogenic and non-carcinogenic risks for both adults and children, with the risk for children being higher.

HACE2-Exosome-Based Nano-Bait for Concurrent SARS-CoV-2 Trapping and Antioxidant Therapy.

Corona Virus Disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is seriously threatening human health. Following SARS-CoV-2 infection, immune cell infiltration creates an inflammatory and oxidative microenvironment, which can cause pneumonia, severe acute respiratory syndrome, kidney failure, and even death. Clinically, a safe and effective treatment strategy remains to be established. Herein, a nano-bait strategy for inhibition of SARS-CoV-2 infection by redirecting viral attack while simultaneously relieving inflammation is developed. Specifically, the nano-bait was based on the exosome-sheathed polydopamine (PDA@Exosome) nanoparticles, which were generated by exocytosis of the PDA nanoparticles from H293T cells. In this approach, PDA@Exosome inherits from the source cells of H293T cells a surface display of ACE2 through pre-engineered expression. The resulting PDA@Exosome can compete with ACE2-expressing epithelial cells for S protein binding, in either the pre-exposure or post-exposure route. Moreover, relying on the ability of PDA to intercept and deactivate radical species, the PDA@Exosome can significantly attenuate the level of inflammatory cytokines by mediating oxidative stress, a major cause of organ injury. Due to its high trapping, multiple antioxidant ability, and good biocompatibility, the HACE2-exosome based nano-bait is a promising robust antiviral nanotherapeutics for the ongoing COVID-19 pandemic.

Antibiotics armed neutrophils as a potential therapy for brain fungal infection caused by chemotherapy-induced neutropenia.

Chemotherapy-induced neutropenia, a symptom of neutrophil depletion, makes cancer patients highly susceptible to invasive fungal infection with substantial morbidity and mortality. To address the cryptococcal brain infection in this condition, this study attempts to arm neutrophils (NEs) with antibiotics to potentiate the antifungal capability of NEs. To allow effective integration, amphotericin B, a potent antibiotic, is assembled with albumin nanoparticles through hydrophobic and hydrogen-bond interactions to form AmB@BSA nanoparticles (A-NPs). The nutrient composition (albumin) and virus-like size (~40 nm) facilitate efficient uptake of A-NPs by NEs to construct the antibiotics-armed NEs. It is demonstrated that the armed NEs can maintain the intrinsic biological functions of NEs, such as cell viability and capacity of migration to an inflammatory site. In a neutropenic mouse model of brain fungal infection, the treatment with the armed NEs allows for preventing fungal invasion more effectively than that with the native NEs, without the apparent systemic toxicity. Such a synergistic anti-infection system maximizes the antifungal effects by taking advantage of NEs and antibiotics. It provides a potential NEs-mediated therapeutic approach for treating fungal infection caused by chemotherapy-induced neutropenia.

Supply-side reform measures of public sports service from the perspective of public health promotion / Reforma da oferta de serviços esportivos públicos do ponto de vista da promoção da saúde pública / Reforma de la oferta de servicios deportivos públicos desde la perspectiva de la promoción de la salud pública

ABSTRACT With the rapid development of public health construction, the supply of public sports services and the demand of residents at this stage are gradually divergent. In order to solve the contradiction between the supply and demand of public sports services, this study, from the perspective of public health, combined with the current situation of demand for public sports services, used the fuzzy analytic hierarchy process to build the supply-side optimization model of public sports services, and to verify it. The results show that the weight of the model optimization index from high to low is service level > resource conditions > public degree > service content > location and type of facilities; the fuzzy comprehensive evaluation result of the model is 85 points, which shows that the index of the optimization model has good applicability. The supply-side optimization model constructed in this study is evaluated by community residents and experts in related fields, so the evaluation results are closer to the residents' demand and have high practical value. The results of this study have reference significance for the supply-side reform of public sports services, and fundamentally ensure the close relationship between the supply-side reform measures and the actual demand of residents.

RESUMO Com o rápido desenvolvimento da construção da saúde pública, a oferta de serviços desportivos públicos e a procura por parte da população, nesta fase, são gradualmente divergentes. A fim de resolver a contradição entre a oferta e a demanda por serviços esportivos públicos, este estudo, sob a perspectiva da saúde pública, combinado com a atual situação da demanda por serviços esportivos públicos por parte da população, utilizou o processo hierárquico analítico difuso para construir o modelo de otimização da oferta de serviços esportivos públicos, e para verificar esse modelo. Os resultados mostram que o peso do índice de otimização do modelo de alto a baixo é o nível de serviço > condições de recursos > Grau público > conteúdo de serviço > localização e tipo de instalações. O resultado de avaliação abrangente difusa do modelo é 85 pontos, o que mostra que o índice do modelo de otimização tem boa aplicabilidade. O modelo de otimização da oferta construído neste estudo é avaliado por residentes da comunidade e especialistas em campos relacionados, de modo que os resultados da avaliação estão mais perto da demanda da população e têm alto valor prático. Os resultados deste estudo têm um significado de referência para a reforma da oferta dos serviços desportivos públicos e garantem fundamentalmente a estreita relação entre as medidas de reforma da oferta e a procura real por parte da população.

RESUMEN Con el rápido desarrollo de la implementación de la salud pública, la oferta de servicios deportivos públicos y la demanda de la población en esta etapa son gradualmente divergentes. Para resolver la contradicción entre la oferta y la demanda de servicios deportivos públicos, se realizó este estudio, desde la perspectiva de la salud pública, combinado con la situación actual de la demanda de servicios deportivos públicos. Para el mismo, se utilizó el proceso de jerarquía analítica difusa para construir el modelo de optimización de la oferta de los servicios deportivos públicos y verificarlo. Los resultados muestran que el peso del índice de optimización del modelo de mayor a menor es el nivel de servicio> las condiciones de los recursos> el grado público> el contenido del servicio> la ubicación y el tipo de instalaciones. El resultado de la evaluación integral difusa del modelo es de 85 puntos, lo que muestra que el índice del modelo de optimización tiene una buena aplicabilidad. El modelo de optimización del lado de la oferta construido en este estudio fue evaluado por habitantes de la comunidad y expertos en campos relacionados, por lo que los resultados de la evaluación están más cerca de la demanda de los residentes y tienen un alto valor práctico. Los resultados de este estudio tienen un significado de referencia para la reforma de la oferta de los servicios deportivos públicos y garantizan fundamentalmente la estrecha relación entre las medidas de reforma de la oferta y la demanda real de los habitantes.

Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice.

In vivo beta-cell neogenesis may be one way to treat diabetes. We aimed to investigate the effect of glucagon-like peptide-1 (GLP-1) on beta-cell neogenesis in type 2 diabetes mellitus (T2DM). Male C57BL/6J mice, 6 wk old, were randomly divided into three groups: Control, T2DM, and T2DM + Lira. T2DM was induced using high-fat diet and intraperitoneal injection of streptozotocin (40 mg/kg/d for 3 d). At 8 wk after streptozotocin injection, T2DM + Lira group was injected intraperitoneally with GLP-1 analog liraglutide (0.8 mg/kg/d) for 4 wk. Apparently for the first time, we report the appearance of a primitive bud connected to pancreas in all adult mice from each group. The primitive bud was characterized by scattered single monohormonal cells expressing insulin, GLP-1, somatostatin, or pancreatic polypeptide, and four-hormonal cells, but no acinar cells and ductal epithelial cells. Monohormonal cells in it were small, newborn, immature cells that rapidly proliferated and expressed cell markers indicative of immaturity. In parallel, Ngn3+ endocrine progenitors and Nestin+ cells existed in the primitive bud. Liraglutide facilitated neogenesis and rapid growth of acinar cells, pancreatic ducts, and blood vessels in the primitive bud. Meanwhile, scattered hormonal cells aggregated into cell clusters and grew into larger islets; polyhormonal cells differentiated into monohormonal cells. Extensive growth of exocrine and endocrine glands resulted in the neogenesis of immature pancreatic lobes in adult mice of T2DM + Lira group. Contrary to predominant acinar cells in mature pancreatic lobes, there were still a substantial number of mesenchymal cells around acinar cells in immature pancreatic lobes, which resulted in the loose appearance. Our results suggest that adult mice preserve the capacity of pancreatic neogenesis from the primitive bud, which liraglutide facilitates in adult T2DM mice. To our knowledge, this is the first time such a phenomenon has been reported.

Ratiometric fluorescence method for ctDNA analysis based on the construction of a DNA four-way junction.

We present a novel ratiometric fluorescent biosensor for ctDNA analysis based on the construction of a DNA four-way junction (FWJ). Three fuel strands for the FWJ are firstly designed and prepared. Another essential strand for the formation of the structure is the DNA product generated from target ctDNA initiated strand displacement amplification. With the transformation of the DNA structure, the FRET states of two fluorophores change and the ratiometric fluorescence response can be recorded to indicate the level of the initial ctDNA. The proposed method also has excellent capability to discriminate mismatches and shows potential practical utility for clinical samples.

Triple-Input Molecular AND Logic Gates for Sensitive Detection of Multiple miRNAs.

Abnormal miRNA expressions are closely related to the occurrence and development of cancers. It is of great significance to monitor miRNA expression levels for early diagnosis and therapy of the diseases. This study presents two independent colorimetric strategies for simultaneously monitoring multiple miRNAs based on cross-linking or non-cross-linking aggregations of gold nanoparticles (AuNPs). By introducing a Y shaped DNA structure and two types of DNA modified AuNPs, a triple-input DNA AND logic gate is facilely developed with the cross-linking aggregation of AuNPs as the signal output. To improve the sensitivity and shorten reaction time, the logic gate is modified by further employing a three DNA strands formed duplex and hybridization chain reaction. Non-cross-linking aggregation of AuNPs is used to evaluate the concentration of initial miRNA inputs. This strategy does not require DNA modification of AuNPs and ultrahigh sensitivity is achieved with the amplification of hybridization chain reaction. The present work may provide powerful tools for multiple miRNAs diagnostics and inspire further development of DNA based logic gates.

Recognition of Helicobacter pylori by protein-targeting aptamers.

BACKGROUND: Helicobacter pylori (H pylori) is a disease-causing pathogen capable of surviving under acidic conditions of the human stomach. Almost half of the world's population is infected with H pylori, with gastric cancer being the most unsatisfactory prognosis. Although H pylori has been discovered 30 years ago, the effective treatment and elimination of H pylori continue to be problematic. MATERIALS AND METHODS: In our study, we screened nucleic acid aptamers using H pylori surface recombinant antigens as targets. Trypsin was used for separating aptamers that were bound to proteins. Following nine rounds of screening, we performed sequence similarity analyses to assess whether the aptamers can recognize the target protein. Two sequences with desirable recognition ability were selected for affinity detection. Aptamer Hp4 with the strongest binding ability to the H pylori surface recombinant antigen was chosen. After optimization of the binding conditions, we conducted specificity tests for Hp4 using Escherichia coli, Staphylococcus aureus, Vibrioanguillarum, and H pylori. RESULTS: The data indicated that the aptamer Hp4 had an equilibrium dissociation constant (Kd ) of 26.48 ± 5.72 nmol/L to the target protein. This aptamer was capable of exclusively detecting H pylori cells, without displaying any specificity for other bacteria. CONCLUSIONS: We obtained a high-affinity aptamer for H pylori, which is expected to serve as a new molecular probe for detection of H pylori.

Experiment and simulation of supersaturated total dissolved gas dissipation: Focus on the effect of confluence types.

Total dissolved gas supersaturation (TDGS) downstream caused by spill discharge from high dams can easily cause fish to suffer from gas bubble disease (GBD). One potential approach to mitigate the impact of TDGS is at the confluence of a downstream tributary, where the introduction of low-TDG water might provide refuge space for fish. In this study, we carried out a series of flume experimental cases and established a three-dimensional TDGS model at confluences. The formula of the dissipation coefficient of TDGS had been obtained by parts of experiment cases. The other parts of experimental cases were carried out to validate the established TDGS model. The biggest relative error of TDG concentration between the experiment and simulation was -5.7%. The results show that the convergence of tributary water (TDG = 100%) can affect the mainstream water (TDG = 140% ∼ 150%) significantly. The two most obvious features are the presence of the separation zone and secondary flow which become more significant as the flow rate increases. The separation zone area at the bottom is smaller than that at the surface. There are two secondary circulations on transversal planes which decrease as the longitudinal distance increases. In addition, the area below 110% and 120% of TDGS in different planes of different cases were compared in detail. This study can provide scientific basis for the utilization of the low-TDG-saturation region to protect fish from the damage of TDGS at confluences during high dam discharge.

Silver nanoparticle@DNA tetrahedron-based colorimetric detection of HIV-related DNA with cascade strand displacement amplification.

DNA tetrahedron-modified silver nanoparticles (AgNPs) were achieved via amino-silver chemistry for the first time and were applied as a colorimetric biosensor for detecting HIV-related DNA. Target DNA initiated strand displacement polymerization and nicking endonuclease-aided cycles were involved to link DNA tetrahedron-modified AgNPs, reporting colorimetric responses. This developed method showed excellent specificity and sensitivity. A wide linear range from 1 to 15 000 nM was achieved with a limit of detection of 0.84 nM. Moreover, it was successfully applied to determine DNA in blood serum samples.