RESUMO
The objective of this study was to evaluate the effect of administering injectable progesterone (P4i) before a timed artificial insemination (TAI) protocol on the follicular growth, ovulation, and pregnancy rate of Bos taurus suckled cows. The effect of P4i administration before the TAI on the pregnancy rate (P/AI) was evaluated in 576 suckled Bos taurus cows at 30-90 days postpartum. In addition, the effect of P4i administration before TAI on follicular dynamics was evaluated in subgroup of 401 suckled Bos taurus cows. On Day -10 (D-10), cows were divided into two experimental groups (Control and P4i). In this moment, P4i cows received i.m. 150 mg of injectable long-action progesterone. After that, both experimental groups received a synchronization protocol (Day 0; D0) that consisted of administration i.m. of 2 mg of estradiol benzoate and a progesterone intravaginal insert on D0. On Day 8 (D8), the progesterone insert was removed, and the cows received 500 µg of cloprostenol, 400 IU of eCG, and 1 mg of estradiol cypionate. TAI was performed 48 h after the removal of the progesterone insert. The ultrasound exams were performed in a subgroup of cows on Days 0, 8, 10 and 12 to evaluate the diameter of the largest follicle, rate of follicular growth and risks of single and double ovulation. The pregnancy diagnosis was performed 30 days after TAI in all cows to determine the pregnancy rate. The diameter of the largest follicle, on D10 (P = 0.84), rate of follicular growth (P = 0.14), ovulation rate (P = 0.40) and double ovulation rates (P = 0.23) did not differ between experimental groups. The pregnancy rate was greater in the P4i group [Control 46.2 % (133/288) vs. P4i 55.6 % (160/288); P = 0.03]. The diameter of the largest follicles (LF) on D0 (Control 11.6 ± 0.2 vs. P4i 13.3 ± 0.3) was greater (P = 0.01) in the P4i group. In conclusion, injectable progesterone before the ovulation synchronization protocol increased the diameter of the largest follicle on the D0 and the pregnancy rate in multiparous Bos taurus suckled beef cows.
Assuntos
Ovulação , Progesterona , Gravidez , Feminino , Bovinos , Animais , Progesterona/farmacologia , Folículo Ovariano , Paridade , Estradiol/farmacologia , Fertilidade , Inseminação Artificial/veterinária , Inseminação Artificial/métodos , Sincronização do Estro/métodosRESUMO
Group B Streptococcus (GBS) is the most common cause of life-threatening infection in neonates. Guidelines from CDC recommend universal screening of pregnant women for rectovaginal GBS colonization. The objective of this study was to compare the performance of a combined enrichment/PCR based method targeting the atr gene in relation to culture using enrichment with selective broth medium (standard method) to identify the presence of GBS in pregnant women. Rectovaginal GBS samples from women at ¡Ý36 weeks of pregnancy were obtained with a swab and analyzed by the two methods. A total of 89 samples were evaluated. The prevalence of positive results for GBS detection was considerable higher when assessed by the combined enrichment/PCR method than with the standard method (35.9% versus 22.5%, respectively). The results demonstrated that the use of selective enrichment broth followed by PCR targeting the atr gene is a highly sensitive, specific and accurate test for GBS screening in pregnant women, allowing the detection of the bacteria even in lightly colonized patients. This PCR methodology may provide a useful diagnostic tool for GBS detection and contributes for a more accurate and effective intrapartum antibiotic and lower newborn mortality and morbidity.
Assuntos
Feminino , Técnicas In Vitro , Streptococcus agalactiae/genética , Streptococcus agalactiae/isolamento & purificação , Metodologia como Assunto , Pacientes , GestantesRESUMO
We report results obtained with sera from 58 chronic chagasic patients that were evaluated for effects on heart rate and atrioventricular (AV) conduction in isolated rabbit hearts and screened for the presence of muscarinic and beta-adrenergic activity. We show that sera from 26 patients decreased heart rate, while 10 increased it and 22 had no effect. Additionally, sera from 20 of the 58 patients blocked AV conduction. Muscarinic activation seems to be involved in both effects, but is not the only mechanism, since atropine did not antagonize the decrease in heart rate in 23 percent of sera or AV block in 40 percent. Sera from patients with complex arrhythmias were significantly more effective in depressing both heart rate and AV conduction. Sera that induce increases in heart rate seem to operate exclusively through beta-adrenergic activation. Two of these sera, evaluated with respect to intercellular communication in primary cultures of embryonic cardiomyocytes were able to block gap junction conductance evaluated by a dye injection technique after 24-h exposure. The mechanisms underlying this uncoupling effect are currently being investigated
Assuntos
Animais , Coelhos , Camundongos , Humanos , Doença de Chagas/sangue , Colinérgicos , Receptores Muscarínicos , Análise de Variância , Nó Atrioventricular , Comunicação Celular , Cardiomiopatia Chagásica , Doença Crônica , Eletrocardiografia , Eletrofisiologia , Estruturas Embrionárias/citologia , Bloqueio Cardíaco , Sistema de Condução Cardíaco , Frequência Cardíaca , Fatores de TempoRESUMO
In this retrospective study, 47 patients with clinical diagnosis of central nervous system metastases of breast cancer were evaluated by computerized tomography (CT), magnetic resonance imaging (MRI) and cerebrospinal fluid (CSF) examination. The patients were divided in 2 groups: 1, without leptomeningeal neoplasm and 2, with leptomeningeal neoplasm. In the group 2, the time interval between the primary disease and the central nervous system metastasis as well as the survival time were shorter than in group 1 (40 and 4.3 months in group 2 versus 57 and 10 months respectively, in group 1). In both groups the most common neurological symptoms and signs were intracranial hypertension and motor deficits. The most sensitive diagnostic methods were CT and MRI in group 1, and the CSF examination in group 2. The use of the tumor markers CEA and CA-15.3 in the routine examination of CSF showed promising results, mainly in leptomeningeal forms.
Assuntos
Neoplasias da Mama/patologia , Neoplasias do Sistema Nervoso Central/secundário , Adulto , Idoso , Cistos Aracnóideos/diagnóstico , Biomarcadores Tumorais/líquido cefalorraquidiano , Neoplasias do Sistema Nervoso Central/líquido cefalorraquidiano , Neoplasias do Sistema Nervoso Central/diagnóstico , Humanos , Neoplasias Meníngeas/diagnóstico , Pessoa de Meia-Idade , Estudos RetrospectivosRESUMO
Scanning and transmission electron microscope studies were performed in the pineal recess region of eighteen adult Didelphis albiventris opossums. With the scanning electron microscope, three concentric regions could be observed in the pineal recess: a central zone lined by cells free of cilia or microvilli, or by a meshwork of interdigitating cell processes; an intermediate zone lined by microvillous cells; and a peripheral zone, lined by ciliated cells. The transmission electron microscope revealed that the central zone is lined mainly by pinealocytes. The lining cells are frequently overlaid by a meshwork of pinealocytic and gliak oricesses. A <
Assuntos
Animais , Epêndima/ultraestrutura , Gambás/anatomia & histologia , Glândula Pineal , Microscopia Eletrônica , Microscopia Eletrônica de VarreduraAssuntos
Cisticercose/epidemiologia , Adolescente , Adulto , Fatores Etários , Idoso , Brasil , Criança , Pré-Escolar , Feminino , Hospitais Gerais , Humanos , Lactente , Pacientes Internados , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores SexuaisRESUMO
1. The activity of choline acetyltransferase (CAT) measured by a radilabeling method was significantly reduced in the heart, submandibular gland and esophagus of rats 20 days after inoculation with Trypanosoma cruzi (Y strain). 2. Normal enzyme activity was recovered in all these organs 100 days after inoculation. 3. In the transcerse colon, no change, 30% reduction and normal activity were found at days 20, 100 and 430 of infection, respectively. 4. The data indicate recovery of parasympathetic function in experimental Chagas' disease. Axonal regrowth and sprouting are discussed as possible candidates for the recovery mechanisms
Assuntos
Ratos , Animais , Masculino , Feminino , Doença de Chagas/enzimologia , Colina O-Acetiltransferase/metabolismo , Esôfago/enzimologia , Glândula Submandibular/enzimologia , Miocárdio/enzimologia , Sistema Nervoso Parassimpático/fisiopatologia , Colo/enzimologia , Regeneração Nervosa , Plasticidade Neuronal , Neurônios/fisiologia , Ratos EndogâmicosRESUMO
Estudou-se a ultra-estrutura do orgao pineal da ave Tinamiforme Crypturellus parvirostris. Ele apresenta uma organizacao folicular com celulas do tipo fotoreceptor rudimentar (celula A) e celulas de sustentacao (celulas B). As primeiras possuem morfologia de celulas fotoreceptoras, com excecao do pediculo basal, que e assinaptico, e do segmento externo, que e atipico e esta representado por um cilio dilatado do tipo 9 + 0 (bulbo-cilio). Assim, apesar do Crypturelus parvirostris pertencer a um grupo de aves considerado muito primitivo, sua pineal nao apresenta caracteristicas morfologicas que indiquem uma atividade fotossensorial pelo menos do tipo convencional. Entretanto, a presenca de um complexo de Golgi bem desenvolvido de cisternas do reticulo endoplasmatico granular muito dilatadas e de abundantes vesiculas indicam a existencia de uma atividade secretora intensa. A presenca de vesiculas revestidas em relacao com a membrana dos processos apicais sugere uma interacao entre estes processos e a substancia na luz dos foliculos
Assuntos
Masculino , Feminino , Animais , Glândula Pineal , AvesRESUMO
O conteudo de noradrenalina do hipotalamo e do tronco encefalico de ratos controle e inoculados con a cepa Y (300.000 tripomastigotas, i.p.) de Trypanosoma cruzi foi medido pela tecnica fluorimetrica de Anton e Sayre. Os animais foram sacrificados 20 e 32 dias depois da inoculacao. Para avaliacao do grau de desnervacao simpatica do coracao dos animais infectados, a auricula direita foi observada com microscopio de fluorescencia apos tratamento histoquimico pela tecnica do acido glioxilico.O conteudo de noradrenalina do hipolatamo e do tronco encefalico dos animais infectados nao diferiu do medido nos animais controle Contudo, um quase completo desaparecimento das fibras adrenergicas foi observado no coracao dos animais chagasicos, sugerindo que o mecanismo envolvido na lesao discrimina neuronios adrenergicos centrais e perifericos
Assuntos
Masculino , Animais , Ratos , Tronco Encefálico , Doença de Chagas , Hipotálamo , NorepinefrinaRESUMO
As concentracoes da LZM foram determinadas nas amostras de LCR normal e patologico; o metodo utilizado foi o turbidimetrico melhorado. A LZM foi mensuravel em todas as amostras normais; apresentou-se aumentada em 60% e 83% dos casos de meningites virais e bacterianas, respectivamente. Foram, tambem observadas elevacoes em neurocisticercose, leucemia linfoblastica aguda, mieloma multiplo e neurocriptococose, nos dois casos de neurolues, a LZM do LCR foi normal