Intracellular expression of pro-inflammatory cytokines (IL-1alpha, TNF-alpha, and IL-6) in chronic hepatitis C.

The study aimed at localizing TNF-alpha, IL-1alpha, IL-6 at light and electron microscope levels in patients with chronic hepatitis C, using the immunocytochemical techniques in biopsy material from patients with chronic hepatitis C and at comparing the expression of the cytokines with histopathological changes. Our studies demonstrated an augmented expression of all cytokines in liver biopsies in chronic hepatitis C, in comparison with respective values, obtained in control biopsy material. The highest expression of the cytokines was observed in hepatocytes. That was confirmed by electron microscopy, which demonstrated the cytokines mainly in altered ER cisterns and in the cytoplasm. In children, the expression of IL-1alpha was negatively correlated with staging, while in adult patients; the staging was positively correlated with the expression of TNF-alpha. The new element involves demonstration of cellular and subcellular expression of TNF-alpha, IL-1alpha and IL-6 in hepatocytes in in vivo infection.

[Evaluation in school anti-tobacco programmes]. / Ewaluacja w szkolnych programach antytytoniowych.

The National Research Institute of Mother and Child (NRIMC) school anti-tobacco programmes are presented. They are discussed with reference to the Centres for Disease Control and Prevention (CDC) guidelines and other effective school programmes to prevent tobacco use among children and young people. The general objectives of the programmes at different educational levels and the elaborated strategic recommendations are presented. The own conceptualised evaluation model of school anti-tobacco programmes is analysed, including process and outcome evaluation. The presented model clarifies concepts and procedures of planning the design and evaluation of the programme in its different stages. In our opinion, the NRIMC evaluation model can be applied in other heath promotion programmes.