The impact of biomolecule interactions on the cytotoxic effects of rhenium(I) tricarbonyl complexes.

Rhenium complexes show great promise as anticancer drug candidates. Specifically, compounds with a Re(CO)3(NN)(py)+ core in their architecture have shown cytotoxicity equal to or greater than that of well-established anticancer drugs based on platinum or organic molecules. This study aimed to evaluate how the strength of the interaction between rhenium(I) tricarbonyl complexes fac-[Re(CO)3(NN)(py)]+, NN = 1,10-phenanthroline (phen), dipyrido[3,2-f:2',3'-h]quinoxaline (dpq) or dipyrido[3,2-a:2'3'-c]phenazine (dppz) and biomolecules (protein, lipid and DNA) impacted the corresponding cytotoxic effect in cells. Results showed that fac-[Re(CO)3(dppz)(py)]+ has higher Log Po/w and binding constant (Kb) with biomolecules (protein, lipid and DNA) compared to complexes of fac-[Re(CO)3(phen)(py)]+ and fac-[Re(CO)3(dpq)(py)]+. As consequence, fac-[Re(CO)3(dppz)(py)]+ exhibited the highest cytotoxicity (IC50 = 8.5 µM for HeLa cells) for fac-[Re(CO)3(dppz)(py)]+ among the studied compounds (IC50 > 15 µM). This highest cytotoxicity of fac-[Re(CO)3(dppz)(py)]+ are probably related to its lipophilicity, higher permeation of the lipid bilayers of cells, and a more potent interaction of the dppz ligand with biomolecules (protein and DNA). Our findings open novel avenues for rational drug design and highlight the importance of considering the chemical structures of rhenium complexes that strongly interact with biomolecules (proteins, lipids, and DNA).

Fatores contextuais do desempenho do atendimento odontológico para gestantes na Atenção Básica entre municípios baianos / Contextual factors of the performance of dental care for pregnant women in Primary Care among municipalities in Bahia

RESUMO Este estudo objetivou analisar os fatores contextuais do desempenho do atendimento odontológico para gestantes na Atenção Básica entre municípios baianos. A série temporal utilizou dados secundários dos 417 municípios, entre 2018 e 2022. O desfecho foi a meta do indicador de proporção de gestantes com atendimento odontológico por município: insuficiente (<60,0%) ou suficiente (≥60,0%). As variáveis contextuais foram: fatores sociodemográficos (Porte Populacional, Índice de Desenvolvimento Humano Municipal, Produto Interno Bruto municipal per capita - PIB, Índice de Gini da renda domiciliar per capita, Índice de Vulnerabilidade Social e Núcleos Regionais de Saúde) e serviço de saúde (Cobertura de Estratégia Saúde da Família - ESF e de Saúde Bucal). Realizou-se regressão de Poisson uni e multivariada (p<0,05). No último quadrimestre de cada ano: 2,9% (2018), 2,4% (2019), 5,3% (2020), 37,2% (2021) e 71,2% (2022) dos municípios atingiram a meta. No terceiro quadrimestre de 2021, os municípios com menor PIB, e em 2021 e 2022, com maior cobertura de ESF, apresentaram maior chance de atingir a meta. Conclui-se que houve aumento da proporção de atendimento odontológico para gestante ao longo dos anos entre os municípios baianos e atingir a meta foi associado a fatores sociodemográficos e de serviço de saúde.

ABSTRACT This study aimed to analyze the contextual factors of the performance of dental care for pregnant women in Primary Care among municipalities in Bahia. The time series used secondary data from 417 municipalities between 2018-2022. The outcome was the target indicator for the proportion of pregnant women with dental care per municipality: insufficient (<60.0%) or sufficient (≥60.0%). The contextual variables were: sociodemographic factors (Population Size, Municipal Human Development Index, municipal Gross Domestic Product per capita - GDP, Gini Index of household income per capita, Social Vulnerability Index and Regional Health Centers) and health service (Coverage of Family Health Strategy - ESF and Oral Health). Poisson regression was performed (p<0.05). In the last four months of each year: 2.9% (2018), 2.4% (2019), 5.3% (2020), 37.2% (2021) and 71.2% (2022) of the municipalities reached the goal. In the third four months of 2021, municipalities with lower GDP, and in 2021 and 2022, with greater ESF coverage, had a greater chance of reaching the target. It is concluded that there was an increase in the proportion of dental care for pregnant women over the years among the municipalities in Bahia, and achieving the goal was associated with sociodemographic and health service factors.

Atuação do cirurgião - dentista da atenção primária à saúde durante a pandemia da covid-19 / Role of primary health care dental surgeons during the COVID-19 pandemic / Papel de los cirujanos dentistas de atención primaria de salud durante la pandemia de COVID-19

Introdução:A Atenção Primária à Saúde é caracterizada como a porta de entrada preferencial do Sistema Único de Saúde, ocupando lugar central no serviço. Todavia, a pandemia da COVID-19 instigou a necessidade de mudanças naorganização do trabalho das equipes de saúde da atenção básica.Objetivo:Neste cenário, torna-se fundamental conhecer a atuação docirurgião-dentista da Atenção Primária à Saúdeno que se refere à oferta dos serviços odontológicos, alterações no processode trabalho e capacidade técnica de ação, frente às condições estabelecidas pela pandemiadaCOVID-19.Metodologia:Trata-se de um estudo de caráter transversal descritivo e abordagem quantitativa, desenvolvido em um município do interior baiano, com 29 cirurgiões-dentistas vinculados àatenção básica. Esses profissionais foram convidados a responder um questionário on-lineque abordou questões relativas ao desempenho dos cirurgiões-dentistas e a condução das ações de saúde bucal frente às medidas restritivas impostas pela pandemiada COVID-19.Resultados:Foi possível identificar que o processo de trabalho e acesso da população aos serviços de odontologia sofreram interferências, expressas através da limitação do acesso da população à saúde bucal, alterações nas técnicas terapêuticas e interrupção das ações em saúde de abrangência coletiva.Conclusões:O presente estudo apontou limitaçõesno processo de trabalho do cirurgião-dentista da Atenção Primária à Saúdedurante a pandemia da COVID-19, como a dificuldade para atuar em equipe interprofissional e atender as proposições estabelecidas pelaPolítica Nacional da Atenção Básica (AU).

Introduction:Primary Health Care is the preferred gateway to the Brazilian Unified Health System, occupying a central place in the service. However, the COVID-19 pandemic instigated the need for changes in the organization of work of primary care health teams.Objective:In this scenario, it is essential to know the performance of dental surgeons in terms ofdental care offer, changes in the work process and of their technical capacity for action, in the face of the conditions established by the pandemic.Methodology:This is a descriptive cross-sectional study with a quantitative approach developed in a city in the countryside of Bahia with 29 dental surgeons linked to primary care. These professionals were invited to answer an online questionnaire that addressed issues related to the performance of dental surgeons and the management of oral health actions in the face of the restrictive measures imposed by the COVID-19 pandemic.Results:It was possible to identify that the pandemic interfered with the work process and the population's access to dental services, expressed through the limitation of the population's access to oral health, changes in therapeutic techniques and interruption of collective health actions.Conclusions:The present study pointed out limitations in the work process of the primary care dentist during the COVID-19 pandemic, such as the difficulty in working in an interprofessional team and meeting the propositions established by the Brazilian National Primary Care Policy in Health (AU).

Introducción: La Atención Primaria de Salud se caracteriza por ser la puerta de entrada preferente al Sistema Único de SaludBrasileño, ocupando un lugar central en el servicio. Sin embargo, la pandemia de COVID-19 instigó la necesidad de cambios en la organización del trabajo de los equipos de salud de atención primaria.Objetivo: En este escenario, es fundamental conocer el desempeño del odontólogo en cuanto, oferta de cuidado dental, cambios enel procesode trabajo, ya su capacidad técnica de actuación, ante las condiciones establecidas por la pandemiaMetodología: Se trata de un estudio descriptivo transversal con enfoque cuantitativo, desarrollado en una ciudad del interior de Bahía, con 29 odontólogos vinculados a la atención primaria. Estos profesionales fueron invitados a responder un cuestionario en línea que abordaba cuestiones relacionadas con la actuación de los odontólogos y la realización de acciones de salud bucal frente a las medidas restrictivas impuestas por la pandemia de la COVID-19.Resultados: Con los resultados fue posible identificar que el proceso de trabajo y el acceso de la población a los servicios odontológicos sufrieron interferencias, expresadas a través de la limitación del acceso de la población a la salud bucal, cambios en las técnicas terapéuticas e interrupción de las acciones de salud colectiva. Conclusiones: El presente estudio señaló limitaciones en el proceso de trabajo del cirujano dentista de la atención en lasaluddurante la pandemia de COVID-19, como la dificultad para trabajar enequipo interprofesional y cumplir con las proposiciones establecidas por la Política Brasileña de Atención Primaria en Salud (AU).

Acanthus mollis Formulations for Transdermal Delivery: From Hydrogels to Emulsions.

Topical formulations of Acanthus mollis L. leaf and the optimization of the release of their active compounds and their topical bioavailability were investigated for the first time. In vitro, the release of active compounds from three formulations-an oil-in-water cream and two hydrogels (Carbopol 940 and Pluronic F-127)-was determined using Franz diffusion cells. Detection and quantification of the compounds was performed via high-performance liquid chromatography with a photodiode array (HPLC-PDA). DIBOA, a bioactive compound of this medicinal plant, exhibited release kinetics of the Weibull model for the Carbopol and Pluronic F-127 formulation, identifying it as a potential active agent to optimize the topical distribution of the formulations. The implications extend to applications in inflammation treatment and tyrosinase inhibition, suggesting that it can make a significant contribution to addressing skin conditions, including melanoma and various inflammatory diseases.

Upconversion rare Earths nanomaterials applied to photodynamic therapy and bioimaging.

Light-based therapies and diagnoses including photodynamic therapy (PDT) have been used in many fields of medicine, including the treatment of non-oncological diseases and many types of cancer. PDT require a light source and a light-sensitive compound, called photosensitizer (PS), to detect and destroy cancer cells. After absorption of the photon, PS molecule gets excited from its singlet ground state to a higher electronically excited state which, among several photophysical processes, can emit light (fluorescence) and/or generate reactive oxygen species (ROS). Moreover, the biological responses are activated only in specific areas of the tissue that have been submitted to exposure to light. The success of the PDT depends on many parameters, such as deep light penetration on tissue, higher PS uptake by undesired cells as well as its photophysical and photochemical characteristics. One of the challenges of PDT is the depth of penetration of light into biological tissues. Because photon absorption and scattering occur simultaneously, these processes depend directly on the light wavelength. Using PS that absorbs photons on "optical transparency windows" of biological tissues promises deeper penetration and less attenuation during the irradiation process. The traditional PS normally is excited by a higher energy photon (UV-Vis light) which has become the Achilles' heel in photodiagnosis and phototreatment of deep-seated tumors below the skin. Thus, the need to have an effective upconverter sensitizer agent is the property in which it absorbs light in the near-infrared (NIR) region and emits in the visible and NIR spectral regions. The red emission can contribute to the therapy and the green and NIR emission to obtain the image, for example. The absorption of NIR light by the material is very interesting because it allows greater penetration depth for in vivo bioimaging and can efficiently suppress autofluorescence and light scattering. Consequently, the penetration of NIR radiation is greater, activating the biophotoluminescent material within the cell. Thus, materials containing Rare Earth (RE) elements have a great advantage for these applications due to their attractive optical and physicochemical properties, such as several possibilities of excitation wavelengths - from UV to NIR, strong photoluminescence emissions, relatively long luminescence decay lifetimes (µs to ms), and high sensitivity and easy preparation. In resume, the relentless search for new systems continues. The contribution and understanding of the mechanisms of the various physicochemical properties presented by this system is critical to finding a suitable system for cancer treatment via PDT.

Forest Biomass as a Promising Source of Bioactive Essential Oil and Phenolic Compounds for Alzheimer's Disease Therapy.

Alzheimer's disease (AD) is the most common neurodegenerative disorder affecting elderly people worldwide. Currently, there are no effective treatments for AD able to prevent disease progression, highlighting the urgency of finding new therapeutic strategies to stop or delay this pathology. Several plants exhibit potential as source of safe and multi-target new therapeutic molecules for AD treatment. Meanwhile, Eucalyptus globulus extracts revealed important pharmacological activities, namely antioxidant and anti-inflammatory properties, which can contribute to the reported neuroprotective effects. This review summarizes the chemical composition of essential oil (EO) and phenolic extracts obtained from Eucalyptus globulus leaves, disclosing major compounds and their effects on AD-relevant pathological features, including deposition of amyloid-ß (Aß) in senile plaques and hyperphosphorylated tau in neurofibrillary tangles (NFTs), abnormalities in GABAergic, cholinergic and glutamatergic neurotransmission, inflammation, and oxidative stress. In general, 1,8-cineole is the major compound identified in EO, and ellagic acid, quercetin, and rutin were described as main compounds in phenolic extracts from Eucalyptus globulus leaves. EO and phenolic extracts, and especially their major compounds, were found to prevent several pathological cellular processes and to improve cognitive function in AD animal models. Therefore, Eucalyptus globulus leaves are a relevant source of biological active and safe molecules that could be used as raw material for nutraceuticals and plant-based medicinal products useful for AD prevention and treatment.

A review of the ethnomedicinal uses, chemistry, and pharmacological properties of the genus Acanthus (Acanthaceae).

ETHNOPHARMACOLOGICAL RELEVANCE: The Acanthus genus belongs to the Acanthaceae family, and its species are distributed in all continents, mainly in tropical and subtropical regions. Several traditional applications are referred to, but few scientific studies validate them. Despite this, studies in animal models corroborate some of its uses in folk medicine, such as anticancer, antidiabetic, anti-inflammatory, and antinociceptive, which encourages the research on plants of this genus. AIM OF THE REVIEW: To our knowledge, this document is the first comprehensive review study that provides information on the geographic distribution, botanical characteristics, ethnomedicinal uses, phytochemicals, and pharmacological activities of some Acanthus species to understand the correlation between traditional uses, phytochemical, and pharmacological activities, providing perspectives for future studies. RESULTS: In traditional medicine, Acanthus species are mainly used for diseases of respiratory, nervous and reproductive system, gastrointestinal and urinary tract, and skin illness. The most used species are A. montanus, A. ilicifolius, and A. ebracteatus. Chemical compounds (125) from different chemical classes were isolated and identified in seven species, mainly from A. ilicifolius, about 80, followed by A. ebracteatus and A. montanus, appearing with a slightly lower number with fewer phytochemical profile studies. Isolated phytoconstituents have been mainly alkaloids, phenylpropanoid glycosides, and phenylethanoids. In addition, aliphatic glycosides, flavonoids, lignan glycosides, megastigmane derivatives, triterpenoids, steroids, fatty acids, alcohols, hydroxybenzoic acids, simple phenols were also cited. Scientific studies from Acanthus species extracts and their phytoconstituents support their ethnomedical uses. Antimicrobial activity that is the most studied, followed by the antioxidant, anti-inflammatory, and anticancer properties, underlie many Acanthus species activities. A. dioscoridis, A. ebracteatus, A. hirsutus, A. ilicifolius, A. mollis, A. montanus, and A. polystachyus have studies on these activities, A. ilicifolius being the one with the most publications. Most studies were essentially performed in vitro. However, the anticancer, antidiabetic, anti-inflammatory and antinociceptive properties have been studied in vivo. CONCLUSION: Acanthus species have remarkable phytoconstituents with different biological activities, such as antioxidant, antimicrobial, anti-inflammatory, antinociceptive, hepatoprotective, and leishmanicidal, supporting traditional uses of some species. However, many others remain unexplored. Future studies should focus on these species, especially pharmacological properties, toxicity, and action mechanisms. This review provides a comprehensive report on Acanthus genus plants, evidencing their therapeutic potential and prospects for discovering new safe and effective drugs from Acanthus species.

Myxobolus freitasi n. sp. (Myxozoa: Bivalvulida), a parasite of the brain of the electric knifefish in the Brazilian Amazon region / Myxobolus freitasi n. sp. (Myxozoa: Bivalvulida), parasita do cérebro do peixe-faca elétrico na Amazônia brasileira

Abstract A total of 30 specimens of the Amazonian electric knifefish, Brachyhypopomus beebei Schultz, 1944 (Gymnotiformes: Hypopomidae), were collected from the Peixe-Boi River in the state of Pará, Brazil (1°06'59" S; 47°18'26" W). Fragments of the brain tissue were extracted for analysis via optical microscopy, and 18 specimens (60%) presented microparasites of the genus Myxobolus, with unequal capsules. The spores were 18.6 µm (17.7-19.8 µm) long and 8.6 µm (8.4-9.0 µm) wide; the largest polar capsule was 13.0 µm (12.4-13.4 µm) long and 5.6 µm (5.3-6.0 µm) wide, and the smallest capsule was 5.0 µm (4.5-5.3 µm) long and 2.5 µm (2.3-2.6 µm) wide. Infected brain fragments were extracted for histological processing and staining with hematoxylin-eosin and Ziehl-Neelsen. Some fragments were conserved in ethanol for molecular genetics analysis. A partial sequence of the 18S DNA gene was obtained from the spores, which did not correspond to any other sequences deposited in GenBank, although it did form a clade with other Myxobolus parasites of the nervous system. The morphological data, together with molecular phylogeny, supported the designation of a new species Myxobolus freitasi n. sp.

Resumo Um total de 30 espécimes do peixe-faca elétrico da Amazônia, Brachyhypopomus beebei Schultz, 1944 (Gymnotiformes: Hypopomidae), foram coletados no rio Peixe-Mani, no estado do Pará, Brasil (1 ° 06'59 "S; 47 ° 18 ' 26 "W). Fragmentos de tecido cerebral foram extraídos para análise em microscopia óptica, sendo que 18 espécimes (60%) apresentavam microparasitos do gênero Myxobolus, com cápsulas desiguais. Os esporos apresentavam 18,6 µm (17,7-19,8 µm) de comprimento e 8,6 µm (8,4-9,0 µm) de largura; a maior cápsula polar tinha 13,0 µm (12,4-13,4 µm) de comprimento e 5,6 µm (5,3-6,0 µm) de largura, e a menor cápsula tinha 5,0 µm (4,5-5,3 µm) de comprimento e 2,5 µm (2,3-2,6 µm) de largura. Fragmentos cerebrais infectados foram extraídos para processamento histológico e coloração com hematoxilina-eosina e Ziehl-Neelsen. Alguns fragmentos foram conservados em etanol para análise genética molecular. Dos esporos, foi obtida uma sequência parcial do gene 18S do DNA, que não correspondeu a nenhuma outra sequência depositada no GenBank, embora tenha formado um clado com outros parasitas do gênero Myxobolus do sistema nervoso. Os dados morfológicos, juntamente com a filogenia molecular, apoiaram a designação de uma nova espécie Myxobolus freitasi n. sp.

Manutenção do aleitamento materno no retorno ao trabalho / Maintenance of breastfeeding in the return to work / Mantenimiento del alcance materno en el retorno al trabajo

Pesquisa foi motivada a investigar como as mulheres trabalhadoras realizava a manutenção do aleitamento materno ao retornar de seu trabalho, que teve como objetivo descrever se havia dificuldades e estratégias realizadas para a manutenção do aleitamento. O método análise dos dados pelo referencial de Bardin, realizada entre os meses de setembro à outubro de 2017, com vinte mulheres entre 19 a 39 anos de idade cadastradas e atendidas na unidade de estratégia saúde da família do município do Rio de Janeiro, sendo produzidas duas categorias: Paradoxo da rede de apoio; Ausência do Enfermeiro no trabalho multiprofissional. Resultado obteve como desmame precoce havendo a introdução de leite pasteurizado como consequência. Conclui-se que há insegurança para a realização de ações de continuidade de aleitamento materno após o retorno ao trabalho.(AU)

The research was motivated to investigate the hard-working women accomplished the maintenance of the maternal breast feeding when returning of his / her work, that he / she had an objective described if there were difficulties and strategies accomplished for the maintenance of the breast feeding. The methodology analysis of the data for the reference of Bardin, conducted between the months of September to October of 2017, twenty women among 19 to 39 years of age registered and assisted in the unit of strategy. municipal district of Rio de Janeiro, being produced two categories: Paradox of the support net; Absence of the Nurse in the multi-professional work. Result obtained how it weans precocious having the introduction of milk pasteurized as consequence. It is ended that there is insecurity for the accomplishment of actions of continuity of maternal breast feeding after the return to the work.(AU)

La investigación fue motivada a investigar cómo las mujeres trabajadoras realizaban el mantenimiento de la lactancia materna al retornar de su trabajo, que tuvo como objetivo describir si había dificultades y estrategias realizadas para el mantenimiento de la lactancia. La metodología análisis de los datos por el referencial de Bardin, realizada entre los meses de septiembre a octubre de 2017, veinte mujeres entre 19 a 39 años de edad registradas y atendidas en la unidad de estrategia salud de la familia del municipio de Río de Janeiro En enero, se producirá dos categorías: Paradoja de la red de apoyo; Ausencia del enfermero en el trabajo multiprofesional. Resultado obtenido como destete precoz habiendo la introducción de leche pasteurizada como consecuencia. Se concluye que hay inseguridad para la realización de acciones de continuidad de lactancia materna después del retorno al trabajo.(AU)

Atomização de polpa de cajá-umbu utilizando maltodextrina como agente carreador em diferentes temperaturas de secagem / Atomization of pulp cajá-umbu using maltodextrin as carrier agent and different drying temperatures

O objetivo do presente trabalho foi obter e caracterizar de polpa de cajá-umbu atomizada por spray-dryer. A cajá-umbu madura passaram pelas etapas de seleção, lavagem, sanitização, enxágue e despolpamento, obtendo-se assim a polpa. Para atomização por spray dryer adicionou-se 15% de maltodextrina e atomizou em três temperaturas de entrada (100, 120 e 160ºC). A polpa de cajá-umbu in natura e atomizadas foram submetidas a análise de umidade, atividade de água (aw), cinzas, sólidos solúveis, acidez, pH, vitamina C e carotenoides. A polpa in natura apresentou maiores teores de carotenoides (16,58 µg·g-1) demonstrando que a atomização influência nos carotenoides, já a polpa atomizada, apresentou menor teor de umidade com o aumento da temperatura de secagem e reduziu a aw. Para obtenção do pó de cajá-umbu indica-se o uso da temperatura de 120°C.

Influência da temperatura de entrada de secagem e concentração de maltodextrina sobre as propriedades físico-química de polpa de acerola em pó / Influence of drying entry temperature and maltodextrin concentration on the physical-chemical properties of powdered powder

O objetivo do presente trabalho foi avaliar a influência da concentração da maltodextrina e da temperatura de entrada de secagem sob as características físico-químicas de polpa de acerola em pó. As acerolas maduras foram selecionadas, lavadas, sanitizadas, enxaguadas e despolpada. As polpas foram divididas em quatro tratamentos, utilizando-se duas concentrações de maltodextrina (14 e 26%) que foram atomizadas em spray dryer em duas temperaturas (128 e 152°C). A polpa em pó e in natura foram analisadas quanto a umidade, SST, ATT, pH e carotenoides. De acordo com os resultados obtidos foi possível verificar que os teores de umidade foram influenciados pela temperatura e concentração de maltodextrina. A acidez da polpa em pó diminui com o aumento da do agente carreador. O teor de carotenoides não apresentou diferença significativa entre os tratamentos estudados. Para o processo de atomização da acerola por spray dryer indica-se o uso de maltodextrina a 26% independente da temperatura.

Myxozoan infection in the muscle layer of the intestine of Rhamdia quelen from the Amazon River Basin, Brazil / Infecção por Myxozoa na camada muscular intestinal do Rhamdia quelen da Bacia do Rio Amazonas, Brasil

ABSTRACT: The present study investigated the morphology and pathology associated with the occurrence of cysts caused by Myxobolus sp. in the intestine of the silver catfish Rhamdia quelen . Comparisons with the other Myxobolus species that infect the intestines of cyprinids and siluriforms revealed that the pyriform spores of Myxobolus sp. are similar in shape to those described previously in M. miyairii, M. duodenalis and M. cunhai, but different in size. Morphometric analyses revealed that mature spores of Myxobolus sp. (10.9 x 5.1μm) are smaller than those of most species except M. cunhai (10.0 x 5.0μm), which has been described infecting the Brazilian catfish Pimelodus clarias , although the spores differ morphologically in relation to the shape of the polar capsules. Further research is necessary in order to clarify the taxonomic and phylogenetic relationships among these congeneric species.

RESUMO: O presente estudo investigou a morfologia e patologia associada com a ocorrência de cistos de Myxobolus sp. no intestino do peixe siluriforme Rhamdia quelen . Comparações com outras espécies de Myxobolus que têm infectado intestinos de carpas e bagres de água doce demonstraram que os esporos piriformes de Myxobolus sp. são similares no formato dos anteriormente descritos para M. miyairii, M. duodenalis e M. cunhai , diferindo, no entanto, quanto ao tamanho. As análises morfométricas revelaram que os esporos maduros de Myxobolus sp. (10,9 x 5,1μm) são menores do que os da maioria das espécies, exceto para M. cunhai (10,0 x 5,0μm), que foi descrito infectando o bagre brasileiro Clarias pimelodus , embora os esporos sejam diferentes morfologicamente em relação à forma das cápsulas polares. Futuras pesquisas são necessárias para esclarecer as relações taxonômicas e filogenéticas entre espécies congêneres.

Lymphocytic meningoencephalomyelitis associated with Myxobolus sp. (Bivalvulidae: Myxozoa) infection in the Amazonian fish Eigenmannia sp. (Sternopygidae: Gymnotiformes) / Meningoencefalomielite linfocitária associada à infecção por Myxobolus sp. (Bivalvulidae:Myxozoa) em peixe amazônico Eigenmannia sp. (Sternopygidae: Gymnotiformes)

Abstract The genus Myxobolus, parasites that infect fishes, which cause myxobolosis, includes spore organisms belonging to the phylum Myxozoa and represents approximately 36% of all species described for the entire phylum. This study describes lymphocytic meningoencephalomyelitis associated with Myxobolus sp. infection in the brain and spinal cord (the central nervous system, CNS) of Eigenmannia sp., from the Amazon estuary region, in the Administrative District of Outeiro (DAOUT), Belém, Pará, Brazil. In May and June 2015, 40 Eigenmannia sp. specimens were captured from this region and examined. The fish were anesthetized, slaughtered and dissected for sexing (gonad evaluation) and studying parasites and cysts; after diagnosing the presence of the myxozoans using a light microscope, small fragments of the brain and spinal cord were removed for histological processing and Hematoxylin-Eosin and Ziehl-Neelsen staining. Histopathological analysis of the brain and spinal cord, based on histological sections stained with Hematoxylin-Eosin, pronounced and diffuse edema in these tissues, and congestion, degeneration, and focal necrosis of the cerebral cortex. The present study describes lymphocytic meningoencephalomyelitis associated with infection by Myxobolus sp. in the central nervous system of Eigenmannia sp.

Resumo O gênero Myxobolus é composto por parasitas esporais que podem infectar peixes e causar a “myxobolose”. São organismos pertencentes ao filo Myxozoa e representam cerca de 36% do total de espécies descritas para todo o Filo. Este estudo descreve meningoencefalomielite linfocitária, associada à infecção por Myxobolus sp. no cérebro e medula espinhal (SNC) de Eigenmannia sp, oriundo de região estuarina amazônica, no Distrito Administrativo de Outeiro (DAOUT), município de Belém, Pará, Brasil. Foram capturados e examinados 40 espécimes de Eigenmannia sp. entre os meses de maio e junho de 2015. Os peixes foram anestesiados, abatidos e dissecados para sexagem (avaliação das gônadas) e pesquisa de parasitos e cistos. Após o diagnóstico da presença dos mixosporidios, utilizando-se microscópio de luz, pequenos fragmentos do cérebro e da medula espinal foram removidos para processamento histológico e coloração por Hematoxilina-Eosina e coloração especial em Ziehl-Neelsen. A análise histopatológica do cérebro e da medula espinhal, com base em cortes histológicos corados com Hematoxilina-Eosina, mostrou edema difuso nesses tecidos, e congestão, degeneração e necrose focal do córtex cerebral. O presente estudo descreve meningoencefalomielite linfocítica, associada à infecção por Myxobolus sp., no sistema nervoso central de Eigenmannia sp.

Lymphocytic meningoencephalomyelitis associated with Myxobolus sp. (Bivalvulidae: Myxozoa) infection in the Amazonian fish Eigenmannia sp. (Sternopygidae: Gymnotiformes).

The genus Myxobolus, parasites that infect fishes, which cause myxobolosis, includes spore organisms belonging to the phylum Myxozoa and represents approximately 36% of all species described for the entire phylum. This study describes lymphocytic meningoencephalomyelitis associated with Myxobolus sp. infection in the brain and spinal cord (the central nervous system, CNS) of Eigenmannia sp., from the Amazon estuary region, in the Administrative District of Outeiro (DAOUT), Belém, Pará, Brazil. In May and June 2015, 40 Eigenmannia sp. specimens were captured from this region and examined. The fish were anesthetized, slaughtered and dissected for sexing (gonad evaluation) and studying parasites and cysts; after diagnosing the presence of the myxozoans using a light microscope, small fragments of the brain and spinal cord were removed for histological processing and Hematoxylin-Eosin and Ziehl-Neelsen staining. Histopathological analysis of the brain and spinal cord, based on histological sections stained with Hematoxylin-Eosin, pronounced and diffuse edema in these tissues, and congestion, degeneration, and focal necrosis of the cerebral cortex. The present study describes lymphocytic meningoencephalomyelitis associated with infection by Myxobolus sp. in the central nervous system of Eigenmannia sp.

Henneguya paraensis n. sp. (Myxozoa; Myxosporea), a new gill parasite of the Amazonian fish Cichla temensis (Teleostei: Cichlidae): morphological and molecular aspects.

The present study describes light microscopy, transmission electron microscopy, and molecular analyses of a myxosporid found parasitizing the gill region of the teleost fish Cichla temensis, collected from the Tocantins River, near Cametá, Pará State, Brazil. The prevalence of infection was 60 %. The spore-containing cysts that were located in the gill lamellae were oval and whitish. The spores had a mean length of 42.3 ± 0.65 µm; fusiform body, 12.8 ± 0.42-µm long and 8.6 ± 0.32-µm wide; each of the two valves exhibited a tapering tail of 29.5 ± 0.73 µm length. The spores had two polar capsules, 7.4 ± 0.16-µm long by 2.6 ± 0.08-µm wide, containing a polar filament with 5-7 twists. The spores differ from the species previously described, and phylogenetic analysis based on spore morphology and molecular aspects indicated that the fish parasite Henneguya sp. has a strong trend to form clades mainly based on the environment and host order/family. Thus, we conclude that the species belongs to the family Myxobolidae, genus Henneguya, which comprises a new species: Henneguya paraensis n. sp.

Association between atypical parathyroid adenoma and neurofibromatosis.

Primary hyperparathyroidism is a disease characterized by excessive production of parathyroid hormone (PTH), which is due to a parathyroid adenoma in 85% of cases. An atypical parathyroid adenoma, with some histopathological features of parathyroid carcinoma, may be found in some of the cases, although it may not fulfill all the criteria for this diagnosis. Neurofibromatosis type 1 (NF1) is an autosomal dominant systemic disease that may be associated with hyperparathyroidism. We report here the rare combination of a patient with NF1 and clinical manifestations of hyperparathyroidism due to an atypical parathyroid adenoma.

Association between atypical parathyroid adenoma and neurofibromatosis

Primary hyperparathyroidism is a disease characterized by excessive production of parathyroid hormone (PTH), which is due to a parathyroid adenoma in 85% of cases. An atypical parathyroid adenoma, with some histopathological features of parathyroid carcinoma, may be found in some of the cases, although it may not fulfill all the criteria for this diagnosis. Neurofibromatosis type 1 (NF1) is an autosomal dominant systemic disease that may be associated with hyperparathyroidism. We report here the rare combination of a patient with NF1 and clinical manifestations of hyperparathyroidism due to an atypical parathyroid adenoma.

Infection by Henneguya sp. (Myxozoa) in the bone tissue of the gill filaments of the Amazonian catfish Hypophthalmus marginatus (Siluriformes).

This study describes aspects of the infection caused by the myxosporean genus Henneguya, which forms cysts in the bony portion of the gill filaments of Hypophthalmusmarginatus. Specimens of this catfish were acquired dead from artisanal fishermen near the town of Cametá, state of Pará, northern Brazil, between July 2011 and May 2012. They were transported in refrigerated containers to the Carlos Azevedo Research Laboratory at the Federal Rural University of Amazonia, in Belém, where analyses were performed. After confirmation of parasitism by the genus Henneguya, observation were made using optical and differential interference contrast (DIC) microscopy. The histological technique of embedment in paraffin was used. Ziehl-Neelsen staining was applied to the histological sections. Necropsy analyses on specimens of H. marginatus showed that 80% of them (40/50) had cysts of whitish coloration inside the bony portion of the gill filaments, filled with Henneguya spores. The present study found inflammatory infiltrate in the vicinity of the cysts. Furthermore, the special Ziehl-Neelsen staining technique made it possible to mark the Henneguya sp. cysts in the bone tissue and in spore isolates in the gill tissue structure. The descriptions of these histopathological findings show that this parasite is very invasive and causes damage to its host tissues.

Morphological aspects of Henneguya aequidens n. sp. (Myxozoa: Myxobolidae) in Aequidens plagiozonatus Kullander, 1984 (Teleostei: Cichlidae) in the Amazon region, Brazil.

A new species of Myxosporea, Henneguya aequidens sp. n. (Myxozoa: Myxobolidae), was described based on its ultrastructural features. This is a parasite of the freshwater fish Aequidens plagiozonatus, in the Peixe-boi River, Pará, Brazil. This parasite was found in the gills, in the form of whitish ellipsoid cysts with mature spores inside them. The average spore body was 15 ± 0.9 µm in length (n = 30) and 6 ± 0.8 µm in width (n = 30), and the tail measured 27 ± 0.5 µm in length (n = 15). The spores showed typical features of the genus Henneguya with two valves of equal size and two symmetrical polar capsules of 3 ± 0.3 µm in length and 2 ± 0.3 µm in width. Each polar capsule had a polar filament forming a helix from the apical region to the polar caps, with four to six turns. Based on the ultrastructural differences in morphology of these spores, the location of the parasite, and its host specificity, this parasite was described as a new species.

Thyroid imaging reporting and data system score combined with Bethesda system for malignancy risk stratification in thyroid nodules with indeterminate results on cytology.

CONTEXT: The thyroid imaging reporting and data system (TI-RADS) was designed to better select patients who had undergone fine-needle aspiration biopsies (FNABs) with high sensitivity and accuracy. However, the combination of TI-RADS scores and Bethesda system categories in indeterminate thyroid nodules has not been examined extensively. OBJECTIVE: This study aimed to stratify indeterminate thyroid nodules (Bethesda categories III, IV and V) according to risk of malignancy as determined by combining TI-RADS score with Bethesda system classification. DESIGN: Retrospective study. Histopathological, cytological and ultrasound (US) data were available for 242 cases after surgery, including 136 indeterminate nodules. METHODS: All thyroid cytopathological slides and US reports were reviewed and classified according to Bethesda system and TI-RADS categories. The malignancy rate was determined for each Bethesda category, TI-RADS score and both methods combined of indeterminate nodules. RESULTS: The malignancy rates were 8·7%, 51·3% and 67·5% for Bethesda categories III, IV and V, respectively. Based on histopathological comparison, the accuracy was 66·7% for TI-RADS greyscale. TI-RADS 3 and 4A scores were observed in 80% of Bethesda III cases, which led to 80% sensitivity and 90% of negative predictive value (NPV). In contrast, for nodules scored as TI-RADS 4B and 5, the combined cytological results of Bethesda IV and V resulted in a higher risk of malignancy (75% and 76·9%, respectively, P < 0·001). CONCLUSIONS: In view of the high NPV of TI-RADS 3/4A only in Bethesda III category, a surgical approach could be considered for lesions defined as Bethesda III, IV and V when TI-RADS 4B and 5 were concomitant.