Assuntos
Biofilmes/crescimento & desenvolvimento , Fímbrias Bacterianas/fisiologia , Bactérias Gram-Positivas/fisiologia , Percepção de Quorum , Adesinas Bacterianas/fisiologia , Aminoaciltransferases/fisiologia , Amilases/fisiologia , Proteínas de Bactérias/fisiologia , Corynebacterium diphtheriae/genética , Corynebacterium diphtheriae/patogenicidade , Corynebacterium diphtheriae/fisiologia , Cisteína Endopeptidases/fisiologia , Ilhas Genômicas/fisiologia , Bactérias Gram-Positivas/patogenicidade , VirulênciaRESUMO
Antitumor activity of Trypanosoma cruzi CH4 strain isolated in Mexico was studied. This parasite is not tumoritropic, but inhibits the growth and metastasizing of solid L5178Y-R lymphoma transplanted to Balb/C mice. Conditioned medium from cell cultures infected with this strain produced a cytostatic effect. Possible mechanisms of this phenomenon are discussed.
Assuntos
Doença de Chagas/complicações , Linfoma de Células T/patologia , Linfoma de Células T/parasitologia , Trypanosoma cruzi , Animais , Proliferação de Células/efeitos dos fármacos , Doença de Chagas/parasitologia , Meios de Cultivo Condicionados/farmacologia , Linfoma de Células T/complicações , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Músculos/parasitologia , Metástase Neoplásica , Carga TumoralRESUMO
A total of 176 sera from 73 patients with verified Lyme borreliosis at different stages of the disease are examined. Serological diagnosis was carried out by 2 methods: indirect immunofluorescence (IIF) with corpuscular B. burgdorferi antigen and enzyme immunoassay (EIA) with purified flagellar B. burgdorferi antigen (Dako). EIA with Dako antigen is more sensitive for the diagnosis of Lyme borreliosis at any period of the disease than IIF. Analysis of correlations between the results of IIF and EIA showed correlation in the levels of IgG but not IgM antibodies. The findings confirmed a previous hypothesis that inadequate antibacterial therapy before investigation decreases the level of antibodies to Borrelia. In patients with a history of Lyme borreliosis, antibodies to B. burgdorferi are detected less frequently by both IIF and EIA. Patients with persistent levels of antibodies to B. burgdorferi, even without clinical signs of infection, are in need of regular check-ups, because the prognostic significance of antibodies to B. burgdorferi is unknown and relapses may occur after months and years.
Assuntos
Técnica Indireta de Fluorescência para Anticorpo , Técnicas Imunoenzimáticas , Doença de Lyme/diagnóstico , Adolescente , Adulto , Idoso , Anticorpos Antibacterianos/análise , Grupo Borrelia Burgdorferi/imunologia , Feminino , Humanos , Imunoglobulina G/análise , Imunoglobulina M/análise , Masculino , Pessoa de Meia-Idade , Prognóstico , RecidivaRESUMO
The suggested scheme of laboratory tests helped identify the etiology of viral encephalitides in 65.9% of cases. Diagnosis of tick-borne encephalitis was confirmed by laboratory findings in 93.6% of cases. Patients with Californian encephalitis were detected in the Sverdlovsk region for the first time. A rather high rate of detection of antibodies to Tahyna virus was revealed in the examined population: up to 60%. Influenza origin of encephalitis was recognized in 6.2% of cases, Lyme's disease was diagnosed in 9.3%.