RESUMO
OBJECTIVE: Proteomics-based approach was applied to analyze and compare the difference of proteins among human nasal inverted papilloma (NIP), nasal polyposis and normal nasal mucosa, in order to screen different proteins as marker. METHODS: The total proteins of NIP, nasal polyposis and normal nasal mucosa were separated by two-dimensional gel electrophoresis (2-DE). Protein image obtained by using the gel of Calibrated GS-800 Densitometer system, and determined different protein spots. RESULTS: Six differential proteins between NIP and nasal polyp tissue were identified, which were galectin-1, Manganese-superoxide dismutase, galectin-7, trichostatin A, prohibitin and transferring. All of them were increased in NIP. CONCLUSIONS: Six differential proteins were possibly involved in NIP, which provided a new way for discriminating NIP from nasal polyposis. The data would be good for the establishment of NIP protein 2-DE map.
Assuntos
Mucosa Nasal/metabolismo , Pólipos Nasais/metabolismo , Neoplasias Nasais/metabolismo , Papiloma Invertido/metabolismo , Proteômica , Eletroforese em Gel Bidimensional , Galectina 1/metabolismo , Humanos , Mucosa Nasal/patologia , Pólipos Nasais/patologia , Neoplasias Nasais/patologia , Papiloma Invertido/patologiaRESUMO
OBJECTIVE: To observe the transcriptional regulation of the two isoflavones genistein and daidzein on target genes. METHODS: In this study, we used ERalpha or ERbeta over-expressing Hela cells to observe the transcriptional regulation of genistein and daidzein on ERE reporter gene with calcium-phosphate method, and furthermore observing the effects of phytoestrogen antagonist ICI 182780 on their activation. RESULTS: Our results showed that both genistein and daidzein could activate ERE receptor gene through ERalpha and ERbeta, and these effects could be blocked by ICI 182780. CONCLUSION: Both genistein and daidzein can mimic estrogen's effect to activate the transcription of target genes through binding to the ERs.