Changes in the Tibial Strain After Proximal Fibular Osteotomy: A Biomechanical Cadaveric Study.

Total knee arthroplasty surgery is an increasingly common procedure for the treatment of uni- and tricompartmental knee osteoarthritis, particularly in advanced stages and in the older population. Its usage is being extended to younger patients, where implant longevity is of concern. In the younger age group, especially with early disease, other options merit consideration. On the other hand, it may not be possible for elderly patients with medical comorbidities to undergo joint replacement surgery. Proximal fibular osteotomy (PFO) has recently been advocated to treat medial knee osteoarthritis. Although there have been clinical reports showing promising outcomes, the biomechanical basis of this procedure is still unclear. We performed a cadaveric study to investigate the effect of PFO on proximal tibial strain. Eight unpaired cadaveric lower limb specimens were loaded in compression at 2 times body weight. Strain gauges were mounted on various sites on the proximal tibia and fibula. After PFO, there was a significant increase in the lateral tibial strain adjacent to the proximal tibiofibular joint (P<.05). There was moderate effect size reduction in the anteromedial tibial strain as well as moderate effect size increase in the posterior tibial strain. The strain reduction seen at the anteromedial tibia can offer a possible explanation for symptomatic relief after PFO. However, the increase in the lateral and posterior tibial strain raises concern about long-term accelerated wear in these regions. [Orthopedics. 2022;45(5):314-319.].

The Relationship of Transepicondylar Width with the Distal and Posterior Femoral Condyles and Its Clinical Implications: A Three-Dimensional Study.

BACKGROUND: Restoration of the anatomical joint line, while important for clinical outcomes, is difficult to achieve in revision total knee arthroplasty (rTKA) due to distal femoral bone loss. The objective of this study was to determine a reliable method of restoring the anatomical joint line and posterior condylar offset in the setting of rTKA based on three-dimensional (3D) reconstruction of computed tomography (CT) images of the distal femur. METHODS: CT scans of 50 lower limbs were analyzed. Key anatomical landmarks such as the medial epicondyle (ME), lateral epicondyle, and transepicondylar width (TEW) were determined on 3D models constructed from the CT images. Best-fit planes placed on the most distal and posterior loci of points on the femoral condyles were used to define the distal and posterior joint lines, respectively. Statistical analysis was performed to determine the relationships between the anatomical landmarks and the distal and posterior joint lines. RESULTS: There was a strong correlation between the distance from the ME to the distal joint line of the medial condyle (MEDC) and the distance from the ME to the posterior joint line of the medial condyle (MEPC) (p < 0.001; r = 0.865). The mean ratio of MEPC to MEDC was 1.06 (standard deviation [SD]: 0.07; range: 0.88-1.27) and that of MEPC to TEW was 0.33 (SD: 0.03; range: 0.25-0.38). CONCLUSIONS: Our findings suggest that the fixed ratios of MEPC to TEW (0.33) and that of MEPC to MEDC (1.06) provide a reliable means for the surgeon to determine the anatomical joint line when used in combination.

The tale of two vessels, vascular complications following a breach of the pelvic inner table due to acetabular screws: a report of two cases.

Injuries to the pelvic vasculature during total hip arthroplasties are rare but have serious consequence. They demand urgent and early identification so that appropriate treatment can be instituted. If the bleeding is severe, cardiovascular compromise occurs intraoperatively and this will alert the surgeon of this possibility during acetabular screw placement. Alternatively, a delay in diagnosis can occur because the bleeding and the injured vessel are in the pelvic cavity and not visualized during the surgery. In this article, we report two cases from our center occurring within a six-month interval that sustained a vascular injury during acetabular drilling for screw placement for cementless cup fixation. Each case had a different vessel injury and different lessons can be learned from these rare injuries. The first case had an injury of the inferior gluteal artery following a breach of the sciatic notch. The vessel was treated with percutaneous embolization. The second case demonstrated a venous injury, following a medial protrusio technique for congenital hip dysplasia and a short anterosuperior screw, transecting the external iliac vein. This was subsequently repaired using an endovascular technique. We conclude the reasons for these vessel injuries after analyzing advanced imaging, discuss measures to avoid vessel injury and detail the minimally invasive method for their treatment.

Fate of tenogenic differentiation potential of human bone marrow stromal cells by uniaxial stretching affected by stretch-activated calcium channel agonist gadolinium.

The role for mechanical stimulation in the control of cell fate has been previously proposed, suggesting that there may be a role of mechanical conditioning in directing mesenchymal stromal cells (MSCs) towards specific lineage for tissue engineering applications. Although previous studies have reported that calcium signalling is involved in regulating many cellular processes in many cell types, its role in managing cellular responses to tensile loading (mechanotransduction) of MSCs has not been fully elucidated. In order to establish this, we disrupted calcium signalling by blocking stretch-activated calcium channel (SACC) in human MSCs (hMSCs) in vitro. Passaged-2 hMSCs were exposed to cyclic tensile loading (1 Hz + 8% for 6, 24, 48, and 72 hours) in the presence of the SACC blocker, gadolinium. Analyses include image observations of immunochemistry and immunofluorescence staining from extracellular matrix (ECM) production, and measuring related tenogenic and apoptosis gene marker expression. Uniaxial tensile loading increased the expression of tenogenic markers and ECM production. However, exposure to strain in the presence of 20 µM gadolinium reduced the induction of almost all tenogenic markers and ECM staining, suggesting that SACC acts as a mechanosensor in strain-induced hMSC tenogenic differentiation process. Although cell death was observed in prolonged stretching, it did not appear to be apoptosis mediated. In conclusion, the knowledge gained in this study by elucidating the role of calcium in MSC mechanotransduction processes, and that in prolonged stretching results in non-apoptosis mediated cell death may be potential useful for regenerative medicine applications.

Three-Dimensional Computed Tomography Analysis of the Posterior Tibial Slope in 100 Knees.

BACKGROUND: The posterior tibial slope (PTS) is an important consideration in knee arthroplasty. However, there is still no consensus for the optimal slope. The objectives of this study were (1) to reliably determine the native PTS in this population using 3-dimensional computed tomography scans and (2) to determine the normal reference range for PTS in this population. METHODS: One hundred computed tomography scans of disease-free knees were analyzed. A 3-dimensional reconstructed image of the tibia was generated and aligned to its anatomic axis in the coronal and sagittal planes. The tibia was then rotationally aligned to the tibial plateau (tibial centroid axis) and PTS was measured from best-fit planes on the surface of the proximal tibia and individually for the medial and lateral plateaus. This was then repeated with the tibia rotationally aligned to the ankle (transmalleolar axis). RESULTS: When rotationally aligned to the tibial plateau, the mean PTS, medial PTS, and lateral PTS were 11.2° ± 3.0 (range, 4.7°-17.7°), 11.3° ± 3.2 (range, 2.7°-19.7°), and 10.9° ± 3.7 (range, 3.5°-19.4°), respectively. When rotationally aligned to the ankle, the mean PTS, medial PTS, and lateral PTS were 11.4° ± 3.0 (range, 5.3°-19.3°), 13.9° ± 3.7 (range, 3.1°-24.4°), and 9.7° ± 3.6 (range, 0.8°-17.7°), respectively. CONCLUSION: The PTS in the normal Asian knee is on average 11° (mean) with a reference range of 5°-17° (mean ± 2 standard deviation). This has implications to surgery and implant design.

Biomechanical analysis of knee laxity with isolated anteromedial or posterolateral bundle-deficient anterior cruciate ligament.

PURPOSE: The purpose of this study was to clarify the changes in the kinematics of the knee that result from isolated deficiency of the anteromedial (AM) or posterolateral (PL) bundle. METHODS: Fourteen cadaveric knees were mounted in a 6-df rig and tested using the following 5 loading conditions: 90-N anterior and posterior tibial loads, 5-Nm internal and external tibial torques, and a simulated pivot-shift test. Tibiofemoral kinematics during flexion-extension was recorded with an optical tracking system for (1) intact knees, (2) knees in which the isolated AM bundle was cut, (3) knees in which the isolated PL bundle was cut, and (4) anterior cruciate ligament (ACL)-deficient knees. The distances between the femoral and tibial attachments of the AM and PL bundles of the ACL were also calculated. RESULTS: Anterior translation laxity under an anterior tibial load, rotational laxity under an internal tibial torque, and anterior translation laxity under pivot-shift loading were significantly different between the knees with AM and PL bundle deficiencies (P < .024), but the changes were small: less than 3 mm or 1.5°. The AM bundle distance increased significantly more after an AM bundle tear (P = .004) than after a PL bundle tear in flexion. Cutting the PL bundle did not have a significant effect on the lengths between the bundle attachments. CONCLUSIONS: An isolated AM or PL bundle tear caused a small increase in laxity (<3 mm or <1.5°). CLINICAL RELEVANCE: If there is a clinically identifiable increase in laxity, then--in addition to the isolated tear of the AM or PL bundle--there must also be a tear of the other bundle of the ACL, or at least a partial tear.

Patellar thickness and lateral retinacular release affects patellofemoral kinematics in total knee arthroplasty.

PURPOSE: To study the effect of increasing patellar thickness (overstuffing) on patellofemoral kinematics in total knee arthroplasty and whether subsequent lateral retinacular release would restore the change in kinematics. METHODS: The quadriceps of eight fresh-frozen knees were loaded on a custom-made jig. Kinematic data were recorded using an optical tracking device for the native knee, following total knee arthroplasty (TKA), then with patellar thicknesses from -2 to +4 mm, during knee extension motion. Staged lateral retinacular releases were performed to examine the restoration of normal patellar kinematics. RESULTS: Compared to the native knee, TKA led to significant changes in patellofemoral kinematics, with significant increases in lateral shift, tilt and rotation. When patellar composite thickness was increased, the patella tilted further laterally. Lateral release partly corrected this lateral tilt but caused abnormal tibial external rotation. With complete release of the lateral retinaculum and capsule, the patella with an increased thickness of 4 mm remained more laterally tilted compared to the TKA with normal patellar thickness between 45° and 55° knee flexion and from 75° onwards. This was on average by 2.4° ± 2.9° (p < 0.05) and 2.°9 ± 3.0° (p < 0.01), respectively. Before the release, for those flexion ranges, the patella was tilted laterally by 4.7° ± 3.2° and 5.4° ± 2.7° more than in the TKA with matched patellar thickness. CONCLUSION: Patellar thickness affects patellofemoral kinematics after TKA. Although lateral tilt was partly corrected by lateral retinacular release, this affected the tibiofemoral kinematics. LEVEL OF EVIDENCE: IV.

Effect of growth differentiation factor 5 on the proliferation and tenogenic differentiation potential of human mesenchymal stem cells in vitro.

The use of growth differentiation factor 5 (GDF-5) in damaged tendons has been shown to improve tendon repair. It has been hypothesized that further improvements may be achieved when GDF-5 is used to promote cell proliferation and induce tenogenic differentiation in human bone marrow-derived mesenchymal stem cells (hMSCs). However, the optimal conditions required to produce these effects on hMSCs have not been demonstrated in previous studies. A study to determine cell proliferation and tenogenic differentiation in hMSCs exposed to different concentrations of GDF-5 (0, 5, 25, 50, 100 and 500 ng/ml) was thus conducted. No significant changes were observed in the cell proliferation rate in hMSCs treated at different concentrations of GDF-5. GDF-5 appeared to induce tenogenic differentiation at 100 ng/ml, as reflected by (1) a significant increase in total collagen expression, similar to that of the primary native human tenocyte culture; (2) a significant upregulation in candidate tenogenic marker gene expression, i.e. scleraxis, tenascin-C and type-I collagen; (3) the ratio of type-I collagen to type-III collagen expression was elevated to levels similar to that of human tenocyte cultures, and (4) a significant downregulation of the non-tenogenic marker genes runt-related transcription factor 2 and sex determining region Y (SRY)-box 9 at day 7 of GDF-5 induction, further excluding hMSC differentiation into other lineages. In conclusion, GDF-5 does not alter the proliferation rates of hMSCs, but, instead, induces an optimal tenogenic differentiation response at 100 ng/ml.

Length-change patterns of the collateral ligaments after total knee arthroplasty.

PURPOSE: Total knee arthroplasty (TKA) is a procedure with function dependent upon correct tensioning of the soft-tissue constraints. The purpose of this study was to examine the length-change behaviour of the collateral ligaments during knee flexion-extension before and after TKA. The influence of differing degrees of internal-external rotation of the femoral component on slackening/tightening of the collateral ligaments during knee flexion was to be studied. METHODS: The length-change patterns of the collateral ligaments were measured in eight intact knees in vitro: sutures were passed along the ligaments and attached to displacement transducers. Measurements were repeated after TKA with the femoral component in neutral rotation, then with 5° internal and 5° external rotation. RESULTS: Both the MCL and LCL slackened during knee flexion from 0° to 110° flexion, at all stages of the experiment. In the native knee, the MCL slackened 2 mm, whilst the LCL slackened 7 mm. The MCL slackened a further 3 mm and the LCL a further 4 mm during flexion post-TKA. A 5° external rotation of the femoral component slackened the MCL 2 mm more and tightened the LCL by 2 mm. The opposite effects resulted from 5° internal rotation. CONCLUSIONS: The collateral ligaments slackened more than normal following TKA, and these length changes were increased by femoral component rotation. External rotation of the femoral component to address patellar tracking may slacken the MCL and thus lead to valgus instability in the flexed knee.

Biomechanical comparisons of knee stability after anterior cruciate ligament reconstruction between 2 clinically available transtibial procedures: anatomic double bundle versus single bundle.

BACKGROUND: Several trials have compared the clinical results between anatomic double-bundle and single-bundle anterior cruciate ligament reconstruction procedures. However, it remains controversial whether the anatomic double-bundle procedure is superior to the single-bundle procedure. HYPOTHESIS: The anatomic double-bundle procedure will be better than the single-bundle procedure at resisting anterior laxity, internal rotation laxity, and pivot-shift instability. STUDY DESIGN: Controlled laboratory study. METHODS: Eight cadaveric knees were tested in a 6 degrees of freedom rig using the following loading conditions: 90-N anterior tibialforce, 5-N.m internal and external tibial torques, and a simulated pivot-shift test. Tibiofemoral kinematics during the flexion-extension cycle were recorded with an optical tracking system for (1) intact, (2) anterior cruciate ligament-deficient knee, (3) anatomic double-bundle reconstruction, and (4) single-bundle reconstruction placed at 11 o'clock in the intercondylar notch. RESULTS: There were significant reductions of anterior laxity of 3.5 mm at 20 degrees of flexion, internal rotational laxity of 2.5 degrees at 20 degrees of flexion, and anterior translations (2 mm) and internal rotations (5 degrees ) in the simulated pivot-shift test in the double-bundle reconstruction com-pared with the single-bundle reconstruction. There were no significant differences between the 2 procedures for external rotation laxity. CONCLUSION: The postoperative anterior translation and internal rotation stability after anatomic double-bundle anterior cruciate ligament reconstruction were significantly better than after single-bundle reconstruction, in both static tests and the pivot shift. CLINICAL RELEVANCE: Unlike previous laboratory studies, this work used clinical arthroscopic methods for anterior cruciate ligament reconstruction, and found that the anatomic reconstruction was superior to a single graft placed at 11 o'clock.

The structural properties of the lateral retinaculum and capsular complex of the knee.

UNLABELLED: Although lateral retinacular releases are not uncommon, there is very little scientific knowledge about the properties of these tissues, on which to base a rationale for the surgery. We hypothesised that we could identify specific tissue bands and measure their structural properties. Eight fresh-frozen knees were dissected, and the lateral soft tissues prepared into three distinct structures: a broad tissue band linking the iliotibial band (ITB) to the patella, and two capsular ligaments: patellofemoral and patellomeniscal. These were individually tensile tested to failure by gripping the patella in a vice jaw and the soft tissues in a freezing clamp. RESULTS: the ITB-patellar band was strongest, at a mean of 582N, and stiffest, at 97 N/mm. The patellofemoral ligament failed at 172 N with 16 N/mm stiffness; the patellomeniscal ligament failed at 85 N, with 13N/mm stiffness. These structural properties suggest that most of the load in-vivo is transmitted to the patella by the transverse fibres that originate from the ITB.

Radiographic features predictive of patellar maltracking during total knee arthroplasty.

Despite improvements in component design and surgical technique, some patients still require lateral retinacular release during TKA to improve patella tracking. We studied 148 fixed-bearing TKAs to identify parameters in pre-operative knee radiographs that would predict intraoperative patellar maltracking. Digital radiographs and software were used to measure coronal alignment, distal femoral valgus angle, proximal tibia varus angle, patellar tilt, patellar shift, Insall-Salvati ratio, and patellar component placement and alignment. Patellar tracking was assessed after all components had been cemented, using both no-touch and modified "towel clip" techniques. The only radiographic parameter independently associated with maltracking was patellar shift. The median pre-operative patellar lateral shift in patients who had maltracking was 4.1 mm compared to 0.0 mm in those who did not. Patients who had a patellar shift of more than 3.0 mm had a high likelihood of maltracking, with estimated positive and negative predictive values of 78 and 95%, respectively. Pre-operative patellar shift may thus be clinically relevant for identifying osteoarthritic patients who have a higher likelihood for patellar maltracking during TKA. Variations in the intrinsic risk for maltracking within patient study populations may account for the widely differing reported rates of patellar maltracking, and our data suggest that information on pre-operative patellar shift may be helpful in stratifying these sample populations.