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1.
FEBS Lett ; 580(17): 4182-7, 2006 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-16828759

RESUMO

Diastolic heart failure (DHF) was produced in 6-day-old piglets by intravenous administration of Doxorubicin, and ANKRD1 protein and mRNA levels were determined in atrial (A) and ventricular (V) chambers of failing vs control hearts. In controls, ANKRD1 showed a left-right (L-R) asymmetric distribution with protein levels 2-fold higher in the LA as compared to the RA, and 8-fold higher in the LV than the RV. In failing hearts, ANKRD1 levels were augmented about 2-fold in each ventricle but equally reduced in both atria as compared to controls. ANKRD1 downregulation in atria is discussed as a process associated with advanced DHF.


Assuntos
Regulação para Baixo , Insuficiência Cardíaca/metabolismo , Proteínas Musculares/biossíntese , Animais , Antibióticos Antineoplásicos/farmacologia , Antibióticos Antineoplásicos/toxicidade , Diástole , Regulação para Baixo/efeitos dos fármacos , Doxorrubicina/farmacologia , Doxorrubicina/toxicidade , Átrios do Coração/metabolismo , Átrios do Coração/patologia , Insuficiência Cardíaca/induzido quimicamente , Insuficiência Cardíaca/patologia , Ventrículos do Coração/metabolismo , Ventrículos do Coração/patologia , Especificidade de Órgãos , Suínos
2.
Cell Tissue Res ; 322(3): 475-89, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16079966

RESUMO

The mechanism of sexualization of the tubular gonad in seawater bivalves is unknown, and no information regarding the genes involved in this process is yet available, except for the identification of esterase (Est)-like "male-associated polypeptide" in the male gonad of Mytilus galloprovincialis. Our present work reveals distinct protein profiles specific for the testicular or ovarian portion of the ovotestis of Pecten maximus. Two proteins exhibiting testis- or ovary-dependent enrichment in the ovotestis have been identified and partially characterized as Est-like and fibronectin (Fn)-like polypeptides, respectively. Immunofluorescence has demonstrated a close association between the localization of these polypeptides and the gonad tubule network and interstitial stroma of the ovotestis of P. maximus. We also present evidence of Est-like and Fn-like protein enrichment, respectively, in testicular and ovarian tissue in hermaphroditic, sex-reversal, and gonochoric species of seawater bivalves. Together, the results (1) strongly suggest that sex-cell-biased expression of Est-like and Fn-like polypeptides in gonad tissue is a widespread phenomenon among bivalve mollusks, despite the high diversification of their sexual patterns, (2) confirm and expand our previous demonstration of sex-biased protein expression in M. galloprovincialis, and (3) indicate a direct link between germ cell differentiation and sexual specialization of the bivalve somatic gonad.


Assuntos
Bivalves/fisiologia , Esterases/metabolismo , Fibronectinas/metabolismo , Diferenciação Sexual/fisiologia , Animais , Bivalves/anatomia & histologia , Bivalves/genética , Bivalves/metabolismo , Western Blotting , Transtornos do Desenvolvimento Sexual , Eletroforese em Gel de Poliacrilamida/métodos , Esterases/genética , Esterases/imunologia , Feminino , Fibronectinas/genética , Fibronectinas/imunologia , Masculino
3.
Eur J Heart Fail ; 6(2): 161-72, 2004 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-14984723

RESUMO

BACKGROUND AND AIM: Cardiac ankyrin repeat protein (CARP), whose expression is down-regulated in response to doxorubicin (Dox) in vitro, has been proposed to be a marker of experimentally-induced cardiac hypertrophy in rodent models. In piglets, the rapid hypertrophy rate of the left ventricle (LV) as compared to that of the right ventricle (RV) represents a natural model of asymmetric ventricular enlargement. We tested whether CARP expression correlates with postnatal ventricular hypertrophy and to what extent CARP can be sensitive to Dox treatment in vivo. METHODS: CARP mRNA and protein levels were quantified (by Northern blot hybridization, semi-quantitative RT-PCR and Western blot) in the piglet heart, both during early postnatal development and upon Dox-induced cardiomyopathy (Dox-CM). RESULTS: The study revealed: (1) significantly augmented CARP mRNA and protein levels in the LV compared to the RV resulting in left vs. right asymmetry in ventricular CARP expression throughout early postnatal development; (2) dose- and chamber-dependent CARP mRNA and protein enrichment in ventricular myocardium in response to Dox; and (3) abolishment of asymmetric patterns of ventricular CARP expression at heart failure resulting from Dox-CM. CONCLUSIONS: (1) CARP is differentially regulated in the LV and RV during both postnatal development and disease; and (2) monitoring of ventricular CARP expression patterns can be used for further analysis of transition from compensated to overt heart failure.


Assuntos
Antibióticos Antineoplásicos/efeitos adversos , Cardiomegalia/tratamento farmacológico , Doxorrubicina/efeitos adversos , Insuficiência Cardíaca/metabolismo , Miocárdio/metabolismo , Proteínas Nucleares/metabolismo , Proteínas Repressoras/metabolismo , Animais , Animais Recém-Nascidos , Antibióticos Antineoplásicos/farmacologia , Antibióticos Antineoplásicos/uso terapêutico , Northern Blotting , Western Blotting , Clonagem Molecular , Doxorrubicina/farmacologia , Doxorrubicina/uso terapêutico , Regulação da Expressão Gênica no Desenvolvimento , Insuficiência Cardíaca/fisiopatologia , Modelos Animais , Proteínas Nucleares/efeitos dos fármacos , Proteínas Nucleares/genética , RNA Mensageiro/metabolismo , Distribuição Aleatória , Proteínas Repressoras/efeitos dos fármacos , Proteínas Repressoras/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Suínos
4.
J Exp Biol ; 206(Pt 2): 313-26, 2003 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-12477901

RESUMO

Our interest in the comparative analysis of male reproductive-tract esterases in different animal groups has led us to undertake a detailed study of the Mytilus galloprovincialis male-associated polypeptide (MAP) throughout the mussel gonad-duct tract and at spawning. The results of this work indicate that MAP is a major protein in M. galloprovincialis semen, with dual presence in both sperm cells and cell-free seminal fluid. Shortly after spawning, the released sperm mass is subdivided in diffused cloudy-like and thread-shaped 'clots', in which a soluble-phase MAP may persist as long as the clots keep their compact form. Additional experiments involving the incubation of spawned spermatozoa at increasing Triton X-100 concentrations demonstrated that MAP is also strongly associated with sperm cells. These results were further validated by immunofluorescent staining, which revealed that MAP is localized in the mid-piece region of spawned spermatozoa. This unexpected finding raises the possibility that MAP may play a role in sperm fertility in bivalves. Using whole-mount histology and micromanipulation techniques, we studied the structural patterning of the mantle gonad-duct network and assessed the sampling of luminal contents from the ducts. Of particular interest is the observation that MAP content in the luminal fluid increases from the lumen of the spermatogenic tubules to that of the collecting gonad ducts, where MAP is detected at a very high concentration. These high levels may lead to a significant presence of MAP in semen and consequently to a prolonged survival of sperm spawned at sea. In addition, data related to the potential structural similarity between mussel MAP and esterase S of the Drosophila virilis ejaculatory bulb are presented and discussed. Finally, we show that the 64kDa protein of human semen reveals positive cross-reactivity with antibodies directed against Mytilus MAP and Drosophila esterase S. Taken together, the results reveal mussel MAP as the only esterase-like protein described so far whose distribution in the gonad and semen can be specifically associated with maturation, transport, emission and survival of spermatozoa outside.


Assuntos
Bivalves/metabolismo , Esterases/metabolismo , Peptídeos/metabolismo , Sêmen/metabolismo , Espermatozoides/metabolismo , Sequência de Aminoácidos , Animais , Bivalves/genética , Reações Cruzadas , Drosophila/genética , Drosophila/metabolismo , Esterases/genética , Esterases/imunologia , Humanos , Masculino , Peptídeos/genética , Peptídeos/imunologia , Água do Mar , Homologia de Sequência de Aminoácidos , Especificidade da Espécie
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