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1.
J Tissue Eng Regen Med ; 14(12): 1763-1778, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32931632

RESUMO

Bioengineered scaffolds derived from the decellularized extracellular matrix (ECM) obtained from discarded animal organs and tissues are attractive candidates for regenerative medicine applications. Tailoring these scaffolds with stem cells enhances their regeneration potential making them a suitable platform for regenerating damaged tissues. Thus, the study was designed to investigate the potential of mesenchymal stem cells tailored acellular bubaline diaphragm and aortic ECM for the repair of full-thickness abdominal wall defects in a rabbit model. Tissues obtained from bubaline diaphragm and aorta were decellularized and bioengineered by seeding with rabbit bone marrow derived mesenchymal stem cells (r-BMSC). Full-thickness abdominal wall defects of 3 cm × 4 cm size were created in a rabbit model and repaired using five different prostheses, namely, polypropylene sheet, nonseeded diaphragm ECM, nonseeded aorta ECM, r-BMSC bioengineered diaphragm ECM, and r-BMSC bioengineered aorta ECM. Results from the study revealed that biological scaffolds are superior in comparison to synthetic polymer mesh for regeneration in terms of collagen deposition, maturation, neovascularization, and lack of any significant (P > 0.05) adhesions with the abdominal viscera. Seeding with r-BMSC significantly increased (P < 0.05) the collagen deposition and biomechanical strength of the scaffolds. The bioengineered r-BMSC seeded acellular bubaline diaphragm showed even superior biomechanical strength as compared to synthetic polymer mesh. Tailoring of the scaffolds with the r-BMSC also resulted in significant reduction (P < 0.01) in antibody and cell mediated immune reactions to the xenogeneic scaffolds in rabbit model.


Assuntos
Parede Abdominal/patologia , Aorta/fisiologia , Bioengenharia , Diafragma/fisiologia , Células-Tronco Mesenquimais/citologia , Regeneração/fisiologia , Alicerces Teciduais/química , Adipogenia , Animais , Fenômenos Biomecânicos , Búfalos , Bovinos , Linhagem da Célula , Condrogênese , Colágeno/metabolismo , DNA/metabolismo , Matriz Extracelular/metabolismo , Implantes Experimentais , Osteogênese , Coelhos , Dodecilsulfato de Sódio , Aderências Teciduais/patologia , Água
2.
Res Vet Sci ; 125: 360-369, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31376718

RESUMO

Alpha-tocopherol-selenium (ATS) and ascorbic acid (AA) are the potent antioxidants. The present study investigated whether supplementation of ATS and AA in periparturient sows has positive effects on amelioration of oxidative stress, serum immunoglobulin G (IgG), lipid profile and sows performance. For this, twenty-four pregnant multiparous sows (landrace×indigenous) were randomly distributed into four groups (6 sows per group) 20 days before expected date of farrowing as Control (basal diet); ATS (basal diet + ATS); AA (basal diet + AA) and ATS-AA (basal diet + ATS plus AA). The results of the study revealed that the concentrations of triglyceride and cholesterol significantly reduced from day -7 to day 7 of farrowing irrespective of supplementations to sows, but the leptin concentration significantly reduced on day 7 of farrowing in ATS-AA supplemented sows (p<0.05). Moreover, sows of supplemented groups experienced decreased oxidative stress and cortisol level than control sows. The serum IgG concentration was significantly increased on day 7 post-farrowing in ATS group but it was much earlier on day 2 of farrowing in ATS-AA group (p<0.001). Supplementing sows with ATS and/or AA did not influence significantly the birth weight, weaning weight and litter size at weaning (p>0.05). Although piglet survival rate was not affected significantly by supplementation, however, piglet mortality rate was lowest in ATS-AA than any other groups. It was concluded that supplementation of ATS and/or AA to sows during late gestating and early lactating period ameliorated oxidative stress, improved lipid profile and serum IgG level without influencing reproductive performance.


Assuntos
Ácido Ascórbico/metabolismo , Metabolismo dos Lipídeos , Estresse Oxidativo , Selênio/metabolismo , Sus scrofa/fisiologia , alfa-Tocoferol/metabolismo , Ração Animal/análise , Animais , Animais Recém-Nascidos/fisiologia , Ácido Ascórbico/administração & dosagem , Contagem de Células Sanguíneas/veterinária , Peso Corporal , Dieta/veterinária , Suplementos Nutricionais/análise , Feminino , Lactação , Leptina/metabolismo , Tamanho da Ninhada de Vivíparos , Longevidade , Gravidez , Selênio/administração & dosagem , alfa-Tocoferol/administração & dosagem
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