Severe Acute Respiratory Syndrome Coronavirus 2 Spreads to Lymph Nodes and Strongly Expands CD4+ Effector Memory RA Cells in a Patient With Mild Coronavirus Disease 2019.

A woman with mild coronavirus disease 2019 developed cervical adenopathy, being diagnosed of Epstein-Barr virus infectious mononucleosis. We performed fine needle aspiration, and demonstrate that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is found in lymph nodes even in mild disease along with a strong expansion of terminally differentiated effector memory CD4+ T cells, a cell population that is practically absent in lymph nodes.

Daclatasvir combined with sofosbuvir or simeprevir in liver transplant recipients with severe recurrent hepatitis C infection.

Daclatasvir (DCV) is a potent, pangenotypic nonstructural protein 5A inhibitor with demonstrated antiviral efficacy when combined with sofosbuvir (SOF) or simeprevir (SMV) with or without ribavirin (RBV) in patients with chronic hepatitis C virus (HCV) infection. Herein, we report efficacy and safety data for DCV-based all-oral antiviral therapy in liver transplantation (LT) recipients with severe recurrent HCV. DCV at 60 mg/day was administered for up to 24 weeks as part of a compassionate use protocol. The study included 97 LT recipients with a mean age of 59.3 ± 8.2 years; 93% had genotype 1 HCV and 31% had biopsy-proven cirrhosis between the time of LT and the initiation of DCV. The mean Model for End-Stage Liver Disease (MELD) score was 13.0 ± 6.0, and the proportion with Child-Turcotte-Pugh (CTP) A/B/C was 51%/31%/12%, respectively. Mean HCV RNA at DCV initiation was 14.3 × 6 log10 IU/mL, and 37% had severe cholestatic HCV infection. Antiviral regimens were selected by the local investigator and included DCV+SOF (n = 77), DCV+SMV (n = 18), and DCV+SMV+SOF (n = 2); 35% overall received RBV. At the end of treatment (EOT) and 12 weeks after EOT, 88 (91%) and 84 (87%) patients, respectively, were HCV RNA negative or had levels <43 IU/mL. CTP and MELD scores significantly improved between DCV-based treatment initiation and last contact. Three virological breakthroughs and 2 relapses occurred in patients treated with DCV+SMV with or without RBV. None of the 8 patient deaths (6 during and 2 after therapy) were attributed to therapy. In conclusion, DCV-based all-oral antiviral therapy was well tolerated and resulted in a high sustained virological response in LT recipients with severe recurrent HCV infection. Most treated patients experienced stabilization or improvement in their clinical status.

Optimización de un medio de cultivo para el crecimiento de Gardnerella vaginalis / Optimization of a cultural medium for growing Gardnerella vaginalis

Se presenta una técnica para la optimización del medio de cultivo Caldo Triptona Soya suplementado con suero equino para el crecimiento de Gardnerella vaginalis. Se utilizó un diseño experimental 2² donde se evaluó la influencia de dos componentes del medio y un diseño compuesto central para mejorar los rendimientos celulares. Con la mejor variante de este estudio se realizaron corridas para caracterizar el crecimiento bacteriano empleando diferentes estimaciones. Se obtuvo que la dirección hacia los mayores crecimientos se localizó hacia mayores concentraciones de triptona soya y suero equino. La variación en la concentración de los componentes evaluados no influyó en la expresión de las proteínas antigénicas dado por los perfiles electroforéticos obtenidos.

The optimization of the cultural medium Soy Triptone Broth supplemented with equine serum to grow Gardnerella vaginalis is presented in this paper. An experimental design 2² was used in which the influence of two components of this medium was evaluated; and a central design aimed at improving cell performances. With the best variant of this study, the runs allowed characterize the bacterial growth using different estimations. The greatest growths were observed in higher concentrations of soy triptone and equine serum. The variation of the evaluated component concentrations did not have an impact on the expression of antigenic proteins given by the obtained electrophoretic profiles.