RESUMO
Polyvinyl-pyrrolidone capped spherical cadmium sulphide quantum dots (CdS-PVP QDs), 2-6 nm in size, were developed as a selective turn-on fluorescence nanosensor for monohydrogen phosphate ion (HPO42-) in aqueous medium. Fluorescence intensity of CdS-PVP QDs significantly increased with addition of HPO42- ions, whereas the other common inorganic ions had very little effect on the fluorescence intensity. The proposed sensor may be efficiently used for the detection of HPO42- ions at a low level of concentration up to 213 nM in real urine sample. Cell imaging study indicates that the CdS-PVP QDs are cell permeable and can detect the intracellular distribution of HPO42- ions under fluorescence microscope. The CdS-PVP QDs showed considerable activity against Staphylococcus aureus also.
Assuntos
Anti-Infecciosos/farmacologia , Compostos de Cádmio/química , Compostos de Cádmio/farmacologia , Fosfatos/análise , Povidona/química , Pontos Quânticos/química , Sulfetos/química , Sulfetos/farmacologia , Teoria da Densidade Funcional , Fluorescência , Humanos , Concentração de Íons de Hidrogênio , Íons , Limite de Detecção , Testes de Sensibilidade Microbiana , Nanopartículas/química , Nanopartículas/ultraestrutura , Tamanho da Partícula , Pós , Salinidade , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Difração de Raios XRESUMO
BACKGROUND:The purpose of the study was to extract carotenoids from thermophilic bacteria which show efficient antioxidant and protein oxidation inhibition properties, characterize and identify those isolates, extract the carotenoids in different solvents, quantify the carotenoids and perform concentration-dependent and solvent-dependent quantitative assays validated and analysed by appropriate statistical tests. METHODS: Three pigment-forming thermophilic strains were isolated from water sample of Paniphala hot spring, India, and tentatively identified by 16S ribosomal DNA (rDNA) homology. Different concentrations of the carotenoid extracts (100, 80, 40 and 20µg) in three solvents, methanol, DMSO and water, were used to determine the antioxidant activity through five methods: the DPPH (1,1-diphenyl-2-picrylhydrazyl) assay, the ABTS (2,2-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid)) assay, the hydrogen peroxide assay, TOC (total antioxidant capacity) assay and inhibition of protein oxidation assay. Statistical analysis of mean, standard deviation, ANOVA and Pearson correlationcoefficient was performed in Microsoft Excel statistical package.Results:The isolates were tentatively identified as Meiothermussp. strain RP, Meiothermussp. strain TP and Thermusstrain YY.Meiothermussp. formed red coloured pigment, where as Thermussp. formed yellow coloured pigment. Allof the extracts showed positive results in DPPH assay, ABTS assay and hydrogen peroxide radical scavenging assaywith best results obtained when the extracts were dissolved in water. Total antioxidant capacity assay was also highin all the extracts. Protein oxidation inhibition activity was only seen in extracts of strain YY. One-way ANOVA(analysis of variance) clearly showed that choice of solvent influenced the antioxidant capacity of all of the extracts. CONCLUSIONS: Newer and efficient antioxidative compounds are constantly being searched for, and the carotenoid extracts of RP, TP and YY have been shown to catalyze various types of antioxidative reactions, including proteinoxidation inhibition by YY. Thus, all these extracts have huge potential to be industrially and pharmaceutically useful.
Assuntos
Produtos da Oxidação Avançada de Proteínas/análise , Bactérias/isolamento & purificação , Carotenoides/biossíntese , Carotenoides/uso terapêuticoRESUMO
Several naphthalene-based aldazine derivatives were developed as efficient colorimetric and fluorescence probes for selective ratiometric recognition of traces of zinc acetate. The derivative structures were characterized by single-crystal X-ray diffraction. The probes were used for in vitro tracking of zinc acetate in endophytic bacteria within rice root tissue and to image zinc acetate in human breast cancer cells (MCF7) by normal and fluorescence microscopy. Density functional theoretical studies were in close agreement with the experimental findings.
Assuntos
Bactérias/isolamento & purificação , Microscopia de Fluorescência , Oryza/microbiologia , Acetato de Zinco/química , Bactérias/química , Cristalografia por Raios X , Corantes Fluorescentes/química , Humanos , Células MCF-7 , Conformação Molecular , Raízes de Plantas/microbiologia , Teoria QuânticaRESUMO
A pyrene-thiophene conjugate has been used for the efficient detection of intracellular irons through the generation of bright green luminescence. Amongst three different probes being reported, it is observed that the efficiency for the naked eye green luminescent detection of iron increases on moving from naphthalene to anthracene to pyrene. The developed technique may be very useful to monitor iron and zinc supplementation as single micronutrients in living systems through the construction of an INHIBIT logic gate.
Assuntos
Antracenos/química , Ferro/análise , Microscopia de Fluorescência , Naftalenos/química , Pirenos/química , Tiofenos/química , Zinco/química , Candida albicans/química , Candida albicans/metabolismo , Cristalografia por Raios X , Íons/química , Conformação Molecular , Espectrometria de FluorescênciaRESUMO
A visible light excitable rhodamine based probe operates as a SCN(-) selective fluorescent "turn-on" sensor for living cell imaging with a detection limit of 0.01 µM, which is much lower than the normal SCN(-) level in the human body. A "lock" and "key" model has been proposed to explain the fluorescence enhancement of the rhodamine probe in the presence of SCN(-).
Assuntos
Corantes Fluorescentes/química , Luz , Rodaminas/química , Espectrometria de Fluorescência , Tiocianatos/análise , Candida albicans/citologia , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/patologia , Humanos , Microscopia de Fluorescência , Fumar , Tiofenos/químicaRESUMO
Ni(2+)-induced intramolecular excimer formation of a naphthalene-based novel fluorescent probe, 1-[(naphthalen-3-yl)methylthio]-2-[(naphthalen-6-yl)methylthio]ethane (L), has been investigated for the first time and nicely demonstrated by excitation spectra, a fluorescence lifetime experiment, and (1)H NMR titration. The addition of Ni(2+) to a solution of L (DMSO:water = 1:1, v/v; λ(em) = 345 nm, λ(ex) = 280 nm) quenched its monomer emission, with subsequent enhancement of the excimer intensity (at 430 nm) with an isoemissive point at 381 nm. The fluorescence lifetime of free L (0.3912 ns) is much lower than that of the nickel(2+) complex (1.1329 ns). L could detect Ni(2+) as low as 1 × 10(-6) M with a fairly strong binding constant, 2.0 × 10(4) M(-1). Ni(2+)-contaminated living cells of plant origin could be imaged using a fluorescence microscope.