RESUMO
Monkey studies were conducted for the preclinical safety assessment of SCH 412499, an adenovirus encoding p21, administered by subconjunctival injection prior to trabeculectomy for postoperative maintenance of the surgical opening. Biodistribution of SCH 412499 was minimal and there was no systemic toxicity. Findings included swollen, partially closed or shut eye(s) and transient congestion in the conjunctiva. A mononuclear cell infiltrate was present in the conjunctiva, choroid and other ocular tissues, but completely or partially resolved over time. Electroretinograms and visual evoked potentials revealed no adverse findings. Thus, the findings are not expected to preclude the clinical investigation of SCH 412499.
Assuntos
Adenoviridae/genética , Inibidor de Quinase Dependente de Ciclina p21/genética , Terapia Genética , Implantes para Drenagem de Glaucoma , Anestesia , Animais , Pressão Sanguínea/fisiologia , Túnica Conjuntiva , Conjuntivite/patologia , Eletrorretinografia , Ensaio de Imunoadsorção Enzimática , Potenciais Evocados Visuais/fisiologia , Olho/patologia , Feminino , Frequência Cardíaca/fisiologia , Injeções , Macaca fascicularis , Masculino , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Distribuição Tecidual , Malha Trabecular , CicatrizaçãoRESUMO
In this overview the current status of the role of veterinary ophthalmology in laboratory animal studies is discussed. Attention is devoted to current regulatory requirements and study construction, examination techniques commonly used in studies, and a consideration of species characteristics that may be important to investigators. Methods for recording data are discussed with examples of recording systems.
Assuntos
Animais de Laboratório , Técnicas de Diagnóstico Oftalmológico/veterinária , Oftalmologia , Papel do Médico , Medicina Veterinária , Animais , Guias de Prática Clínica como AssuntoRESUMO
A 6-year-old intact male cynomolgus monkey of Chinese origin was received at the Sierra Biomedical Facility. While physical examination revealed good body condition with no abnormalities, routine ophthalmic examination revealed bilateral proliferative optic neuropathy involving the dorsal aspect of the optic disc. No changes were noted in the appearance of the lesions over 8 months, and fluoroescein angiography revealed no abnormalities other than obstruction of the view of the retinal vessels by the lesions. Histopathologic studies revealed characteristics consistent with a diagnosis of bilateral neuroepithelial choristoma.