Morphometric changes in C57BL/6 mice retina infected by Toxoplasma gondii ME 49 strain.

This study evaluated the morphometric implications in C57BL/6 mouse retina infected by Toxoplasma gondii, ME 49 strain. Twenty C57BL/6 female mice were divided into group 1 (n=8, intraperitoneally infected with 30 cysts of T. gondii ME 49 strain) and group 2 (n=12 non-infected controls). The eyes were enucleated on the 60th day after infection, fixed and processed for light microscopy. Changes in retinal thickness and in the perimeter/area ratio (P/A) of the retinal layers were analyzed by digital morphometry. We considered that P/A was the measurement of retinal architecture distortion induced by toxoplasmosis. This study considered the ganglion cells and nerve fiber layers as a monolayer, thus six layers of retina were evaluated: photoreceptors (PRL), outer nuclear (ONL), outer plexiform (OPL), inner nuclear (INL), inner plexiform (IPL) and ganglion cells/nerve fiber monolayer (GNL). Histological analysis of infected mouse retina showed inflammatory infiltrate, necrosis, glial reaction and distortion of the retina architecture. It also presented increased thickness (167.8±24.9µm versus 121.1±15.4µm, in controls) and increased retinal thickness within the retinitis foci (187.7±16.6µm versus 147.9±12.2µm out of the retinitis foci). A statistically significant difference in P/A was observed between infected and uninfected mouse retinas. The same was observed in PRL, OPL, INL and GNL. Retinal morphometry may be used to demonstrate differences between infected and uninfected mouse retinas.

Comportamento do lactato sanguíneo de pacientes com síndrome isquêmica aguda nas 48 horas de internação na unidade coronariana submetidos à reabilitação cardíaca fase I / Behaviour of sanguineous lactate in pacients with acute ischemic syndrome among 48 hours of in-patient (phase I) cardiac reabilitation in coronary unit

Avaliou-se a influência do exercício precoce pós Síndrome Isquêmica Aguda (SIA) no nível do lactato sanguíneo, nas 48 horas de evolução dos pacientes em unidade coronariana. Foram medidos 12 indivíduos com idade média de 69,4 ± 12,7 anos, sendo 8 do sexo masculino e 4 do sexo feminino, submetidos à análise laboratorial por coleta de amostra sanguínea, antes e após a reabilitação cardíaca na fase 1, segundo o protocolo estabelecido pelo American College of Sports Medicine (ACSM), dentro das 48 horas da admissão na referida unidade. Não houve diferença significativa (p < 0,05) dos níveis de lactato obtidos no período de 48 horas da admissão pós SIA, demonstrando que o exercício físico precoce na fase aguda desta síndrome impediu o pico do lactato em torno das 48 horas de evolução.

Was evaluated the effect of early exercise post Acute Ischemic Syndrome (AIS) on the level of the blood lactate within 48 hours of evolution of patients in the coronary unit. The data collection, which included 12 AIS patients, 69,4 ± 12,7 years old, being 8 males and 4 females, subject to laboratorial analyses by means of blood sample collection before and after the cardiac rehabilitation in phase 1, in compliance with the protocol established by American College of Sports Medicine (ACMS), within the 48 hours of admission in the mentioned unit. There was no significant difference (p < 0,05) on the levels obtained in the period of 48 hours of admission post AIS showing that the early exercise in the acute phase of this syndrome prevented the lactate peak close to the 48 hours of evolution.