RESUMO
Rheumatologists are often confronted by patients with muscle weakness and elevated creatine kinase (CK) levels. Myositis cannot always be determined to be the cause of the complaints. This article presents two cases from our hospital where the diagnosis could only be determined by muscle biopsy. In the first case the patient presented with muscle weakness, pathological weight loss and a significant increase in CK levels. A muscle biopsy revealed an immune-mediated necrotizing myopathy (IMNM) caused by anti-3-hydroxy-3-methyl-gulatryl-CoA reductase (HMG-CoA reductase) autoantibodies due to the intake of statins. The second patient presented with cramp-like and burning muscle pain and weakness of the extremities without a relevant increase in CK level. Myoadenylate deaminase deficiency was also detected by muscle biopsy, and further confirmed by genetic testing.
Assuntos
Creatina Quinase/sangue , Debilidade Muscular , Miosite , Autoanticorpos/imunologia , Doenças Autoimunes , Humanos , Debilidade Muscular/sangue , Debilidade Muscular/diagnóstico , Doenças Musculares/sangue , Doenças Musculares/diagnóstico , Miosite/sangue , Miosite/diagnóstico , NecroseRESUMO
We report the case of a 32-year-old woman with severely elevated serum creatine kinase (CK; 80,000 U/l) and progressive proximal pareses. As muscular biopsy showed inflammatory infiltrates, polymyositis was suspected and immunosuppressive treatment was initiated. However, clinical improvement could not be achieved. Gene sequencing of the DYSF-gene showed a previously unreported homozygous mutation. In summary, elevated serum CK and inflammatory infiltrates in the muscle biopsy are not specific for polymyositis, but may also occur in degenerative diseases (muscular dystrophy), such as dysferlinopathy.
Assuntos
Disferlina/genética , Imunossupressores/uso terapêutico , Distrofia Muscular do Cíngulo dos Membros/diagnóstico , Polimiosite/diagnóstico , Adulto , Biópsia , Creatina Quinase/sangue , Análise Mutacional de DNA , Diagnóstico Diferencial , Feminino , Homozigoto , Humanos , Músculo Esquelético/patologia , Distrofia Muscular do Cíngulo dos Membros/tratamento farmacológico , Distrofia Muscular do Cíngulo dos Membros/genética , Distrofia Muscular do Cíngulo dos Membros/patologia , Polimiosite/tratamento farmacológico , Polimiosite/genética , Polimiosite/patologia , Análise de Sequência de DNA , Falha de TratamentoRESUMO
Central dopaminergic (DA) systems are affected during human immunodeficiency virus (HIV) infection. So far, it is believed that they degenerate with progression of HIV disease because deterioration of DA systems is evident in advanced stages of infection. In this manuscript we found that (a) DA levels are increased and DA turnover is decreased in CSF of therapy-naïve HIV patients in asymptomatic infection, (b) DA increase does not modulate the availability of DA transporters and D2-receptors, (c) DA correlates inversely with CD4+ numbers in blood. These findings show activation of central DA systems without development of adaptive responses at DA synapses in asymptomatic HIV infection. It is probable that DA deterioration in advanced stages of HIV infection may derive from increased DA availability in early infection, resulting in DA neurotoxicity. Our findings provide a clue to the synergism between DA medication or drugs of abuse and HIV infection to exacerbate and accelerate HIV neuropsychiatric disease, a central issue in the neurobiology of HIV.
Assuntos
Dopamina/metabolismo , Infecções por HIV/metabolismo , Infecções por HIV/patologia , Transmissão Sináptica/fisiologia , Ácido 3,4-Di-Hidroxifenilacético/líquido cefalorraquidiano , Adulto , Benzamidas , Antígenos CD4/metabolismo , Estudos de Casos e Controles , Quimiocina CCL2/metabolismo , Galactosefosfatos/metabolismo , HIV/genética , Infecções por HIV/líquido cefalorraquidiano , Infecções por HIV/diagnóstico por imagem , Infecções por HIV/imunologia , Ácido Homovanílico/líquido cefalorraquidiano , Humanos , Masculino , Pessoa de Meia-Idade , Pirrolidinas , Tomografia Computadorizada de Emissão de Fóton Único/métodos , Tropanos , Carga Viral/métodosRESUMO
Myopathies with pathological protein aggregates comprise a numerically significant group of sporadic and hereditary muscle disorders. A rare disease entity within the group of protein aggregate myopathies is the myosin storage myopathy, which is caused by heterozygous mutations in the MYH7 gene which encodes the slow/beta-myosin heavy chain. We report the clinical, myopathological and MRI findings in the first German patient suffering from a myosin storage myopathy due to a heterozygous R 1845W missense mutation.
Assuntos
Miosinas Cardíacas/genética , Doenças Metabólicas/genética , Doenças Metabólicas/metabolismo , Doenças Musculares/genética , Doenças Musculares/metabolismo , Cadeias Pesadas de Miosina/genética , Miosinas/genética , Miosinas/metabolismo , Adulto , DNA/genética , Análise Mutacional de DNA , Humanos , Cartilagem Hialina/patologia , Imageamento por Ressonância Magnética , Masculino , Doenças Metabólicas/patologia , Músculo Esquelético/patologia , Doenças Musculares/patologia , Mutação de Sentido Incorreto/genéticaRESUMO
The diagnosis "muscular dystrophy" without analysis of the underlying gene defect is nowadays obsolete. With the discovery and cloning of cytoskeleton proteins and intermediate filaments in the muscle fiber membrane, the sarcoplasm and the nucleus which are essential for the normal muscle fiber function, the classification of muscular dystrophies has dramatically improved. Muscular dystrophies are a group of clinically and genetically heterogeneous disorders. By means of immunohistochemistry and molecular genetics more than 40 different disease forms can be distinguished, which are characterised by distinct protein defects or defined gene loci and can be related to typical phenotypes. It is noteworthy that muscular dystrophies may be associated with cardiomyopathy with increased risk of sudden cardiac death. Thus, diagnosis and treatment require experienced investigators and clinicians and regular cardiologic follow-ups, preferably in a specialised muscle center.
Assuntos
Distrofias Musculares/genética , Distrofias Musculares/patologia , Biópsia , Cardiomiopatias/classificação , Cardiomiopatias/genética , Cardiomiopatias/patologia , Aberrações Cromossômicas , Mapeamento Cromossômico , Análise Mutacional de DNA , Morte Súbita Cardíaca/patologia , Genes Recessivos/genética , Loci Gênicos/genética , Genótipo , Humanos , Imuno-Histoquímica , Proteínas Musculares/genética , Músculo Esquelético/patologia , Distrofias Musculares/classificação , Miocárdio/patologia , FenótipoRESUMO
In this report we present the 9-year course of disease in a woman of Turkish origin suffering from Behçet's disease with parenchymal CNS involvement combined with myalgia. Differential diagnoses, such as drug-induced neuromyopathy were excluded on the basis of electromyographic and bioptic tests. We were able to arrest progression of symptoms of both the myopathy and the Neuro-Behçet's disease by means of increased combined immunosuppressive therapy.
Assuntos
Síndrome de Behçet/diagnóstico , Encefalopatias/diagnóstico , Doenças Neuromusculares/diagnóstico , Neurite Óptica/diagnóstico , Uveíte Posterior/diagnóstico , Adulto , Azatioprina/efeitos adversos , Azatioprina/uso terapêutico , Síndrome de Behçet/tratamento farmacológico , Síndrome de Behçet/patologia , Biópsia , Encefalopatias/tratamento farmacológico , Encefalopatias/patologia , Ciclosporina/efeitos adversos , Ciclosporina/uso terapêutico , Relação Dose-Resposta a Droga , Quimioterapia Combinada , Eletromiografia , Feminino , Seguimentos , Humanos , Imunossupressores/efeitos adversos , Imunossupressores/uso terapêutico , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/patologia , Doenças Neuromusculares/tratamento farmacológico , Doenças Neuromusculares/patologia , Neurite Óptica/tratamento farmacológico , Neurite Óptica/patologia , Prednisona/efeitos adversos , Prednisona/uso terapêutico , Uveíte Posterior/tratamento farmacológico , Uveíte Posterior/patologiaRESUMO
Nemaline myopathies (NM) are a rare group of muscle disorders, but represent one of the most common forms of congenital myopathy. The clinical picture ranges from severe muscular hypotonia often leading to death during childhood to mild forms with long life expectancy. Diagnosis is made by muscle biopsy showing characteristic sarcoplasmic and sometimes intranuclear rod bodies. So far, disease-associated mutations have been detected in six genes without any simple correlation between genotype and phenotype or histological findings. We report a patient with a phenotype typical of congenital onset nemaline myopathy and exclusively intranuclear rods. Mutation analysis revealed a new heterozygous missense mutation in exon 3 of the ACTA1 gene (Q139H). Molecular modelling predicts that substitution of Q139 for H139 alters the amino acid side chains and hydrogen bonding which may alter the nucleotide binding cleft by adding 'bulk' to the mutated molecule. Two-dimensional gel electrophoresis demonstrates that mutant actin Q139H is expressed at approximately half the level of wild-type actin in the patient's muscle. We speculate that these alterations, although not directly affecting the nuclear export signal, negatively interfere with the nuclear export of the mutated protein and thereby cause retention of mutant actin and intranuclear rod formation.
Assuntos
Actinas/genética , Corpos de Inclusão Intranuclear/patologia , Músculo Esquelético/patologia , Mutação , Miopatias da Nemalina/genética , Miopatias da Nemalina/patologia , Análise Mutacional de DNA , Feminino , Glutamina/genética , Histidina/genética , Humanos , Lactente , Corpos de Inclusão Intranuclear/ultraestrutura , Microscopia Eletrônica de Transmissão , Modelos Moleculares , Músculo Esquelético/ultraestruturaRESUMO
Three unrelated, sporadic patients with muscle coenzyme Q10 (CoQ10) deficiency presented at 32, 29, and 6 years of age with proximal muscle weakness and elevated serum creatine kinase (CK) and lactate levels, but without myoglobinuria, ataxia, or seizures. Muscle biopsy showed lipid storage myopathy, combined deficiency of respiratory chain complexes I and III, and CoQ10 levels below 50% of normal. Oral high-dose CoQ10 supplementation improved muscle strength dramatically and normalized serum CK.
Assuntos
Erros Inatos do Metabolismo/complicações , Músculo Esquelético/enzimologia , Doenças Musculares/etiologia , Ubiquinona/análogos & derivados , Adulto , Coenzimas , Creatina Quinase/sangue , Parto Obstétrico , Progressão da Doença , Complexo I de Transporte de Elétrons/deficiência , Complexo III da Cadeia de Transporte de Elétrons/deficiência , Feminino , Humanos , Ácido Láctico/sangue , Metabolismo dos Lipídeos , Masculino , Debilidade Muscular/etiologia , Músculo Esquelético/metabolismo , Doenças Musculares/tratamento farmacológico , Doenças Musculares/patologia , Doenças Musculares/fisiopatologia , Gravidez , Complicações na Gravidez/etiologia , Complicações na Gravidez/fisiopatologia , Ubiquinona/deficiência , Ubiquinona/uso terapêuticoRESUMO
Late-onset Pompe's disease, a generalized lysosomal glycogen storage disease caused by acid maltase deficiency, usually presents as a slowly progressive muscular weakness of proximal muscles in lower limbs, followed by involvement of respiratory muscles. In the case presented here, however, respiratory failure was the first and selective symptom which caused the uncommon appearance of a patient entering our outpatient clinic on foot carrying his own artificial respirator. Intercostal muscle biopsy eventually led to the diagnosis.
Assuntos
Doença de Depósito de Glicogênio Tipo II/complicações , Doença de Depósito de Glicogênio Tipo II/diagnóstico , Debilidade Muscular/diagnóstico , Insuficiência Respiratória/diagnóstico , Insuficiência Respiratória/etiologia , Músculos Respiratórios/patologia , Adulto , Doença de Depósito de Glicogênio Tipo II/terapia , Humanos , Masculino , Debilidade Muscular/etiologia , Doenças Raras/diagnóstico , Doenças Raras/etiologiaAssuntos
Osteosclerose/etiologia , Polineuropatia Paraneoplásica/etiologia , Plasmocitoma/complicações , Polirradiculoneuropatia Desmielinizante Inflamatória Crônica/etiologia , Sacro/diagnóstico por imagem , Neoplasias da Coluna Vertebral/complicações , Adulto , Humanos , Imunoglobulinas Intravenosas/uso terapêutico , Masculino , Metilprednisolona/uso terapêutico , Polineuropatia Paraneoplásica/tratamento farmacológico , Polineuropatia Paraneoplásica/terapia , Troca Plasmática , Plasmocitoma/diagnóstico por imagem , Plasmocitoma/patologia , Plasmocitoma/radioterapia , Polirradiculoneuropatia Desmielinizante Inflamatória Crônica/tratamento farmacológico , Polirradiculoneuropatia Desmielinizante Inflamatória Crônica/terapia , Radiografia , Sacro/patologia , Neoplasias da Coluna Vertebral/diagnóstico por imagem , Neoplasias da Coluna Vertebral/patologia , Neoplasias da Coluna Vertebral/radioterapiaRESUMO
The basal ganglia, structures rich in the neurotransmitter dopamine, are primarily affected during human immunodeficiency virus (HIV) infection. The authors measured levels of dopamine and its metabolites, homovanillic acid and 3,4-dihydroxyphenylacetic acid, in brains of uninfected and simian immunodeficiency virus (SIV)-infected rhesus monkeys during the asymptomatic stage of the infection. Moreover, the authors investigated changes in cyclic adenosine monophosphate (cAMP) and cAMP response element-binding protein (CREB), two factors involved in the signaling pathway of dopamine. The brain regions examined were the nucleus accumbens and the corpus amygdaloideum, which are limbic structures of the basal ganglia that are involved in the pathophysiology of psychiatric disorders and substance abuse. Dopamine content was reduced in both regions of SIV-infected monkeys compared to uninfected animals. Moreover, dopamine deficits were associated with a decrease in expression of total CREB. Intracellular concentrations of cAMP were decreased in nucleus accumbens and remained unchanged in corpus amygdaloideum of SIV-infected macaques. Changes in dopamine signaling were not related to pathology or viral load of the investigated animals. The results suggest that dopamine defects precede neurologic deficits and implicate dysfunction of the dopaminergic system in the etiopathogenesis of HIV dementia. Therefore, affective complications in HIV subjects should not be interpreted only as reactive psychological changes. The alterations in the mesolimbic dopaminergic system during asymptomatic stage of SIV infection implicate a biological background for psychiatric disorders in HIV infection.
Assuntos
Encéfalo/metabolismo , AMP Cíclico/metabolismo , Dopamina/metabolismo , Transdução de Sinais/fisiologia , Síndrome de Imunodeficiência Adquirida dos Símios/fisiopatologia , Ácido 3,4-Di-Hidroxifenilacético/análise , Ácido 3,4-Di-Hidroxifenilacético/metabolismo , Animais , Encéfalo/patologia , Encéfalo/virologia , Cromatografia Líquida de Alta Pressão , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo , Dopamina/análise , Eletroforese em Gel de Poliacrilamida , Ácido Homovanílico/análise , Ácido Homovanílico/metabolismo , Immunoblotting , Imuno-Histoquímica , Macaca mulatta , Síndrome de Imunodeficiência Adquirida dos Símios/patologia , Vírus da Imunodeficiência Símia/fisiologiaRESUMO
We report the clinical and bioptic findings for a 57-year-old woman with severe chloroquine-induced myopathy. Since 1989, she had been suffering from systemic lupus erythematosus (SLE) with renal involvement and undergone periods of treatment with azathioprine and cyclophosphamide. Additional therapy with chloroquine (CQ) was started because of arthralgia. At the same time, slightly increased creatine kinase (CK) levels were noted. Myositis was suspected, and the patient was treated with steroids. The CK increase persisted, however, and she developed progressive muscular weakness and muscular atrophy. Routine controls revealed markedly elevated CK levels of 1,700 U/l. The neurological and electrophysiological findings were not typical of myositis. Thus, muscle biopsy of the deltoid muscle was performed in order to exclude polymyositis or toxic myopathy. As it revealed chloroquine-induced myopathy, medication was stopped. Discriminating between primary SLE-induced affection of the musculoskeletal system and drug-induced side effects is important for appropriate treatment of SLE patients.
Assuntos
Creatina Quinase/sangue , Lúpus Eritematoso Sistêmico/sangue , Lúpus Eritematoso Sistêmico/diagnóstico , Músculo Esquelético/patologia , Doenças Musculares/diagnóstico , Antirreumáticos/efeitos adversos , Cloroquina/efeitos adversos , Diagnóstico Diferencial , Feminino , Humanos , Lúpus Eritematoso Sistêmico/tratamento farmacológico , Microscopia Eletrônica , Pessoa de Meia-Idade , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/enzimologia , Doenças Musculares/sangue , Doenças Musculares/induzido quimicamente , Miofibrilas/efeitos dos fármacos , Miofibrilas/ultraestruturaRESUMO
Hypertrophy of the cricopharyngeal muscle is a serious clinical condition that can cause severe dysphagic symptoms, including prolonged deglutition and postdeglutitive aspiration. Although the therapeutical concepts are well established, the pathogenic mechanism of cricopharyngeal hypertrophy remains unclear. We present a patient with a ten-year history of progressive dysphagia. The neurological and MRI findings were normal. However, videocineradiography showed severe hypertrophy of the cricopharyngeal muscle. This condition was first treated by injections of botulinum toxin, which did not alleviate the symptoms. Next, myotomy and muscle biopsy were performed. Histological evaluation disclosed lymphoplasmacellular florid myositis, single-fiber atrophy, and muscle fiber necrosis with phagocytosis. There were no signs of inclusion body myositis or oculopharyngeal muscular dystrophy. Our finding of severe cricopharyngeal muscle hypertrophy associated with myositis has been published previously (n = 34). The study presented here shows cricopharyngeal dysphagia associated with various systemic diseases, including motor neuron disease, general granulomatous disease, dermatomyositis, or inclusion body myositis. Isolated changes of the cricopharyngeal muscle were described in 65% of the cases.
Assuntos
Transtornos de Deglutição/etiologia , Miosite/complicações , Músculos Faríngeos/patologia , Idoso , Feminino , Humanos , Hipertrofia/etiologiaRESUMO
Electron microscopic, fluorescence microscopic, and immunohistochemical studies earlier performed on archival cerebral tissue from Max Bielchowsky's original three patients revealed curvilinear bodies rich in subunit C of mitochondrial ATP synthase (SCMAS). Recent progress in the elucidation of CLN2, i.e. identification of the defective lysosomal enzyme tripeptidyl-peptidase I (TPP-I) and mutations in the CLN2 gene have further corroborated earlier data. Immunohistochemically the absence of the TPP-I protein could be confirmed in the archival tissues using pathological controls. Unlike biochemistry, immunohistochemistry enables examination of these archival tissues elucidating the causative defect. Complementary molecular studies identified mutations in the CLN2 gene in the archival tissues and thereby convincingly demonstrated that these three children truly had classic late infantile neuronal ceroid lipofuscinosis (LINCL), now called CLN2. This archival study documents the possibilities to revalidate disease-specific original nosologic reports. Chloroquine is toxic to lysosomal enzymes and results in lysosomal storage. The material is autofluorescent and gives the ultrastructural pattern of curvilinear profiles, thus resembling classic late infantile NCL, representing a good experimental model. In humans chloroquine therapy may cause a myopathy (and retinopathy) and, as recently suggested, an encephalopathy marked by lysosomal accretion in several cell types including neurons. Immunohistochemically, SCMAS also accumulates, further strengthening morphologic similarity between LINCL and human chloroquine intoxication.
Assuntos
Encéfalo/patologia , ATPases Mitocondriais Próton-Translocadoras , Lipofuscinoses Ceroides Neuronais/patologia , Peptídeo Hidrolases/genética , Aminopeptidases , Criança , Dipeptidil Peptidases e Tripeptidil Peptidases , Endopeptidases/análise , Feminino , Humanos , Imuno-Histoquímica , Masculino , Mutação , Lipofuscinoses Ceroides Neuronais/enzimologia , Lipofuscinoses Ceroides Neuronais/genética , ATPases Translocadoras de Prótons/análise , Serina Proteases , Tripeptidil-Peptidase 1RESUMO
A 47-year-old Greek man presented with a 4-week history of speech difficulties. CT and MRI revealed a low-density multilobulated cystic frontal mass with peripheral ring contrast enhancement adjacent to the sylvian fissure. Examination was normal. Blood tests revealed leucocytosis (16,000 cells/microl) and an elevated erythrocyte sedimentation rate (30/52). A malignant brain tumour was suspected and surgically removed. Histological examination disclosed intracerebral neurocysticercosis.
Assuntos
Encefalopatias/diagnóstico , Neoplasias Encefálicas/diagnóstico , Glioblastoma/diagnóstico , Neurocisticercose/diagnóstico , Encefalopatias/parasitologia , Encefalopatias/patologia , Neoplasias Encefálicas/patologia , Diagnóstico Diferencial , Glioblastoma/patologia , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Neurocisticercose/patologia , Tomografia Computadorizada por Raios XRESUMO
A 13-year-old girl was referred to our clinic because of a positive rheumatoid factor test, muscle pain and weakness. Laboratory evaluation revealed an increased ESR, hypergammaglobulinaemia, antinuclear antibodies, circulating immune complexes, complement consumption and elevated serum creatine kinase (CK) activity. A needle biopsy of the dolent muscle showed normal routine histology. Immunohistochemistry disclosed single lymphocytes and a weak myocytic HLA class I expression. The diagnosis of myositis was considered and corticosteroids were initiated, leading to an increase of complement levels and a decrease of CK-activity and ESR. She subjectively felt stronger but still reported exercise intolerance and metabolic myopathy was considered. Myophosphorylase activity was completely lacking, establishing the diagnosis of McArdle's disease. CK level was found to be elevated in an obese 4-year-old brother too, who refused extensive walking but reported no muscle pain. Myophosphorylase deficiency was demonstrated by histochemistry and by biochemical analysis of his muscle. The female case illustrates that in children with the clinical picture of inflammatory myopathy and serological but not clinical response to therapy underlying metabolic muscle disorders should be excluded. Since the pathogenesis of polymyositis remains unclear, we speculate that inflammatory changes observed in the muscles may have been initiated by muscular damage resulting from the underlying metabolic disease. The serological changes remained unexplained and may contribute to a so far undeterminable connective tissue disease.
Assuntos
Doença de Depósito de Glicogênio Tipo V/diagnóstico , Miosite/diagnóstico , Adolescente , Diagnóstico Diferencial , Feminino , HumanosRESUMO
In 1995 Laing et al. (Am J Hum Genet 56(1995)422) described a single family with nine members affected by an autosomal dominant infantile onset distal myopathy. This family generated a LOD score of 2.6 for a locus on chromosome 14. We describe two families with an infantile onset distal myopathy: a new family with four affected members and the family previously described by Scoppetta et al. (Acta Neurol Scand 92(1955)122) in both of which haplotype segregation was compatible with linkage to the same chromosome 14 locus, generating LOD scores of 0.9 at a penetrance of 100% for the markers D14S283 and D14S64 (theta=0) in both families. The loci for autosomal recessive hereditary inclusion body myopathy and Nonaka myopathy on chromosome 9 and for autosomal dominant distal myopathy of Markesberry-Griggs and Udd on chromosome 2q31-33 were excluded by linkage analysis. The disease followed a uniform course with selective wasting of the anterior tibial muscles, starting in infancy and recognizable by a characteristic clinical sign of the 'hanging big toe'. This was followed by slow progression, with involvement of the finger and wrist extensor muscles in the third decade and proximal limb muscles in the fourth decade. Interestingly, we also found evidence of an accompanying mild peripheral neuropathy in the oldest individual with hypomyelination of numerous large myelinated fibres. In addition, this patient's muscle biopsy also showed autophagic vacuoles and numerous intranuclear tubulo-filamentous inclusions of 15-20 nm diameter. Given that all three families with infantile onset distal myopathy are compatible with linkage to the same locus on chromosome 14, this study supports evidence for, and enlarges the clinical and neuropathological spectrum of the distal myopathy on chromosome 14.
Assuntos
Cromossomos Humanos Par 14/genética , Genes Dominantes/genética , Distrofias Musculares/genética , Adolescente , Adulto , Criança , Pré-Escolar , Mapeamento Cromossômico , Feminino , Ligação Genética/genética , Haplótipos , Humanos , Masculino , Músculo Esquelético/patologia , Músculo Esquelético/fisiopatologia , Músculo Esquelético/ultraestrutura , Distrofias Musculares/patologia , LinhagemRESUMO
Somatic gene therapy with the herpes simplex virus type I thymidine kinase gene/ganciclovir (HSV-Tk/GCV) system and murine retroviral vector producer cells (VPCs) was introduced as a new adjuvant treatment modality to treat tumor bulk and to prevent tumor recurrence in patients harboring malignant glioma. The single-center experience after treatment of 27 patients undergoing tumor resection followed by intracerebral VPC injection for HSV-Tk suicide gene therapy will be presented focused on findings of systematic and close MRI follow-up and a few histological specimens. The data indicate that hemorrhagic necrosis due to endothelial cell transfection mediated vessel necrosis and that local inflammatory immune response occurs frequently after gene therapy. These phenomena seem to be specific because none of the patients of a control group showed any similar features. The prognosis (time to progression, survival) of the patients with "bystander effects" after gene therapy was better, but compared to those patients without bystander effects, they were also privileged by a favorable constellation of prognostic factors. Therefore, the appearance of these neuroradiologic features cannot serve as an indicator for treatment effectiveness and outcome.
Assuntos
Neoplasias Encefálicas/terapia , Encéfalo/irrigação sanguínea , Efeito Espectador , Encefalite/etiologia , Ganciclovir/uso terapêutico , Terapia Genética/efeitos adversos , Glioma/terapia , Herpesvirus Humano 1/enzimologia , Timidina Quinase/genética , Transfecção/métodos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Encéfalo/patologia , Neoplasias Encefálicas/diagnóstico , Encefalite/diagnóstico , Encefalite/imunologia , Feminino , Vetores Genéticos , Glioma/diagnóstico , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Neovascularização Patológica/diagnóstico , Retroviridae/genéticaRESUMO
Therapy of hepatitis B virus (HBV)-associated poly-arteritis nodosa is still evolving. Here we report a successful treatment with a short-term steroid administration in combination with a-interferon and lamivudine and a complete sequence analysis of the HBV genome. A 58-year-old man presented with the symptoms of mononeuritis multiplex associated in time with the onset of highly replicative hepatitis B. Polyarteritis nodosa was confirmed by biopsy. During an initial course with alpha-interferon and prednisolone no clinical improvement or hepatitis B virus seroconversion was observed. After addition of lamivudine to the protocol with fast tapering of prednisolone, HBV DNA fell to undetectable levels within 1 month and liver transaminases normalized. After 6 months of treatment HBeAg seroconversion took place, followed by HBsAg seroconversion 2 months later. Clinical symptoms of polyarteritis improved. No relapse of polyarteritis or hepatitis B was seen during the follow up of 9 months. Complete sequence analysis of the HBV genome revealed 6 nucleotide mutations but none in a relevant antigenic epitope. The present protocol of short-term prednisolone administration combined with alpha-interferon and lamivudine was effective for the treatment of HBV-related polyarteritis nodosa and may be a promising new therapeutic approach.
Assuntos
Antivirais/uso terapêutico , Hepatite B/complicações , Hepatite B/tratamento farmacológico , Interferon-alfa/uso terapêutico , Lamivudina/uso terapêutico , Poliarterite Nodosa/tratamento farmacológico , Poliarterite Nodosa/etiologia , Prednisolona/uso terapêutico , Sequência de Bases , Biópsia , Primers do DNA , DNA Viral/genética , DNA Viral/isolamento & purificação , Quimioterapia Combinada , Genoma Viral , Hepatite B/fisiopatologia , Antígenos E da Hepatite B/sangue , Vírus da Hepatite B/genética , Vírus da Hepatite B/isolamento & purificação , Humanos , Testes de Função Hepática , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Poliarterite Nodosa/patologiaRESUMO
Muscle fiber diameter and fiber-type distribution were analyzed during callus distraction. The right tibia in 24 beagles was lengthened 2.5 cm by callus distraction after osteotomy and application of a ring fixator. Distraction was started at the fifth postoperative day, at a rate of two times for 0.5 mm per day. Twelve dogs that underwent limb-lengthening and three dogs in the control group that did not undergo limb-lengthening were killed at the end of the 25-day distraction phase (group A). The remaining dogs (12 that underwent limb-lengthening and three that did not) were killed after an additional consolidation period of 25 days (group B). The tibialis anterior, extensor digitorum longus, peroneus longus, and gastrocnemius muscles were removed from the right limb (which had undergone distraction) and the left control side of each animal. Crosscut cryostat sections were stained by adenosine triphosphatase at pH 4.3 and 9.4 to determine the size and distribution of types I and II fibers. Morphometric analysis of the muscle fibers was performed by a computer-assisted two-point technique. On the lengthened side, the muscles revealed marked atrophy affecting predominantly type-II fiber in the dogs in group A and affecting both fiber types in dogs in group B. Fiber density increased in both groups. In addition, fiber-type grouping indicative of reinnervation was obvious in group B. Fiber-type distribution in the dogs in group B showed a shift toward type I in the tibialis anterior (p = 0.043) and extensor digitorum longus (p = 0.034) muscles and a shift toward type II in the gastrocnemius (p = 0.038). The data show that tension-stress during tibial lengthening leads to atrophy of type-II fiber, reflecting disuse of muscle fiber in the distraction period as well as neurogenic atrophy followed by the reinnervation processes. Furthermore, the data are consistent with the occurrence of histoneogenesis during limb-lengthening resulting in an increase in fiber density.