RESUMO
This study was conducted to determine effects of pre-synchronization of ovulation timing among heifers and delayed fixed-time artificial insemination (TAI) with sex-sorted semen on proportion of heifers pregnant after TAI (PR/AI). Heifers were assigned to one of eight treatments: 1 and 2), 7-d CO-Synchâ¯+â¯CIDR treatment regimen with administration of gonadotropin-releasing hormone and a CIDR insert on Day 0, prostaglandin F2α (PGF) at CIDR removal on Day 7, and TAI occurring 54â¯h later with conventionally processed (CTRL54-CNV) or sex-sorted semen (CTRL54-SEX); 3 and 4), same as CTRL54 but TAI delayed to 72â¯h with conventionally processed (CTRL72-CNV) or sex-sorted semen (CTRL72-SEX); 5 and 6), same as CTRL54 but additional administration of PGF on Day -7 and TAI with conventionally processed (PRE54-CNV) or sex-sorted semen (PRE54-SEX); 7 and 8), same as PRE54 treatments but TAI delayed to 72â¯h with conventionally processed (PRE72-CNV) or sex-sorted semen (PRE72-SEX). Proportion of heifers pregnant after TAI was greater (Pâ¯≤⯠0.02) with conventionally processed semen compared with sex-sorted semen, yet PR/AI did not differ (Pâ¯=⯠0.14) between heifers in PRE72-CNV and PRE72-SEX groups. There were greater PR/AI in the PRE72-SEX (Pâ¯=⯠0.03) than CTRL54-SEX group (46.1 % and 36.9 %) and there was no difference (Pâ¯=⯠0.31) in PR/AI between CTRL54-CNV and PRE72-SEX groups (50.4 % and 46.1 %). In conclusion, pre-synchronization of ovulation timing among heifers combined with delayed TAI resulted in increased PR/AI with sex-sorted semen compared with the 7-d CO-Synch+CIDR treatment regimen.
Assuntos
Bovinos , Sincronização do Estro/métodos , Inseminação Artificial/veterinária , Ovulação/fisiologia , Pré-Seleção do Sexo/veterinária , Animais , Dinoprosta/administração & dosagem , Dinoprosta/análogos & derivados , Dinoprosta/farmacologia , Estradiol/farmacologia , Feminino , Hormônio Liberador de Gonadotropina/farmacologia , Masculino , Gravidez , Progesterona/farmacologia , Prostaglandinas F/administração & dosagem , Prostaglandinas F/farmacologiaRESUMO
We hypothesized that both day of gestation and maternal nutrition would alter the relative mRNA expression of neutral and acid AA transporters , , , , and . Crossbred Angus heifers ( = 49) were synchronized, bred via AI, assigned to nutritional treatment (100% of NRC requirements for 0.45 kg/d gain [control heifers {CON}] and 60% of CON [restricted heifers {RES}]), and ovariohysterectomized on d 16, 34, or 50 of gestation ( = 6 to 9/d). Nonbred, nonpregnant (NB-NP) controls were ovariohysterectomized on d 16 of the estrous cycle ( = 6) after synchronization. The resulting arrangement was a 2 × 3 factorial + 1 (CON vs. RES × d 16, 34, or 50 + NB-NP controls). Tissues collected included caruncular endometrium (CAR), intercaruncular endometrium (ICAR), fetal membranes (FM; chorioallantois; d 16 and 34), cotyledonary placenta (COT; d 50 only), intercotyledonary placenta (ICOT; d 50 only), and amnion (AMN; d 50 only]). Relative expression of , , , , and was determined for each tissue using NB-NP CAR and NB-NP ICAR tissues for the baseline; for FM, endometrium from NB-NP controls served as the baseline. In CAR, no day × treatment interaction was observed ( > 0.05). However, day of gestation affected relative expression of , where expression on d 16 was greater ( < 0.01) than expression on d 34 and 50. Additionally, relative expression of and was greater ( ≤ 0.05) in pregnant heifers compared with NB-NP heifers. For ICAR, was influenced by a day × treatment interaction ( < 0.01), where expression in d 16 RES was greater ( ≤ 0.05) than that of any other day or nutritional treatment. Furthermore, expression in d 16 CON was greater ( ≤ 0.05) than that in d 50 RES, with those in d 34 CON and RES and d 50 CON being intermediate. In addition, was affected by day of gestation, where expression on d 16 was greater ( < 0.01) than that on d 34 and 50. A day × treatment interaction was not observed ( > 0.05) in FM; however, expression on d 34 was greater ( = 0.02) than on d 50, with that on d 16 being intermediate. Day of gestation also affected expression of , where expression on d 34 and 50 was greater ( < 0.01) than that on d 16. These data support our hypothesis in that both day of gestation and maternal nutrition affected the relative mRNA expression of AA transporter in ICAR, whereas day of gestation has a greater effect on the relative mRNA expression of other neutral and acidic AA transporters in the various tissues studied.
Assuntos
Sistemas de Transporte de Aminoácidos Acídicos/genética , Bovinos/fisiologia , Animais , Cruzamento , Bovinos/genética , Endométrio/fisiologia , Ciclo Estral , Feminino , Fenômenos Fisiológicos da Nutrição Materna , Placenta/fisiologia , Gravidez , RNA Mensageiro/genética , Útero/fisiologiaRESUMO
We hypothesized that maternal nutrition and day of gestation would impact utero-placental mRNA expression of the nutrient transporters , , , , and in beef heifers. Crossbred Angus heifers (n = 49) were estrous synchronized, bred via AI, assigned to nutritional treatment (CON = 100% of NRC requirements for 0.45 kg/d gain and RES = 60% of CON) and ovariohysterectomized on d 16, 34, or 50 of gestation (n = 6 to 9/d); Non-bred, non-pregnant (NB-NP) controls were fed the CON diet, not bred, and were ovariohysterectomized on d 16 of the synchronized estrous cycle = 6). The resulting arrangement of treatments was a 2 × 3 factorial + 1 (CON vs. RES × d 16, 34, or 50 + NB-NP controls). Caruncle (CAR), intercaruncular endometrium (ICAR), and fetal membranes (FM [chorioallantois]), were obtained from the pregnant uterine horn (the uterine horn containing the conceptus) immediately after ovariohysterectomy. On d 50 cotyledons (COT), intercotyledonary placenta (ICOT) and amnion (AMN) were also collected. Relative expression of nutrient transporters was determined for each tissue utilizing NB-NP-CAR and NB-NP-ICAR tissues as the baseline. For FM, NB-NP endometrium served as the baseline. There was no interaction of day × treatment ( ≥ 0.20) for any genes in CAR. However, CAR expression of was greater ( < 0.01) on d 16 compared with d 34 and 50, and , , and were greater ( ≤ 0.05) on d 34 compared with d 16 and 50. In ICAR, was the only gene to be influenced by the day × treatment interaction ( = 0.01), being greater in d 50 CON compared with d 34 CON and d 16 and 50 RES. In ICAR, expression of was greater ( < 0.01) on d 16 compared with d 34, and expression of was greater ( < 0.01) on d 34 and 50 compared with d 16. In FM, expression of was greater ( = 0.04) on d 16 compared with d 50 of gestation, and expression of was greater ( < 0.01) on d 34 and 50 compared with d 16. On d 50, expression of , , and expression were all greater ( < 0.05) in AMN compared with COT and ICOT, and expression of was greater ( < 0.01) in ICOT compared with COT and AMN. These data indicate that day was a more influential factor for mRNA expression of utero-placental glucose and cationic AA transporters than maternal nutritional status in heifers during early pregnancy.
Assuntos
Sistemas de Transporte de Aminoácidos Básicos/metabolismo , Bovinos/fisiologia , Frutose/metabolismo , Glucose/metabolismo , Fenômenos Fisiológicos da Nutrição Materna , Animais , Cruzamento , Dieta/veterinária , Endométrio/metabolismo , Ciclo Estral/metabolismo , Feminino , Placenta/metabolismo , Gravidez , Útero/metabolismoRESUMO
Glucose transporter solute carrier family 2 member 14 () is a duplicon of glucose transporter solute carrier family 2 member 3 () with a 95% shared homology to and has not previously been isolated in ruminant uteroplacental tissues. The transporter has been previously isolated in Holstein heifer uterine epithelium but not in ovine epithelium. We hypothesized that and its duplicon would be found in bovine uteroplacental tissues and that maternal nutrition and day of gestation would impact mRNA expression of and . Crossbred Angus heifers ( = 49) were estrus synchronized, bred via AI, and assigned to nutritional treatment (CON = 100% of requirements to gain 0.45 kg/d; RES = 60% of CON) at breeding. Ovariohysterectomy was performed on d 16, 34, or 50 of gestation ( = 6 to 9/d); nonpregnant (NP) controls were not bred and ovariohysterectomized on d 16 of the synchronized estrous cycle ( = 6). The resulting treatment arrangement was a 2 × 3 factorial + 1. Uteroplacental tissues (caruncle, CAR; intercaruncular endometrium, ICAR; and fetal membrane [chorioallantois], FM) were obtained from the pregnant uterine horn immediately after ovariohysterectomy. For NP controls, only CAR and ICAR were obtained. There were no day × treatment interactions for or gene expression in CAR, ICAR, or FM. Expression of in CAR was greater ( = 0.03) on d 50 compared with d 16. In ICAR, was greatest ( = 0.02) on d 50 compared with d 16 and 34 of gestation. In FM, was greater ( = 0.04) on d 16 compared with d 50. Expression of was greater ( = 0.05) in pregnant compared with nonpregnant heifers. Additionally, expression of was greater ( = 0.01) on d 34 and 50 compared with d 16. Expression of in CAR was greater ( = 0.03) on d 50 compared to d 16 and 34. In CAR, tended ( = 0.07) to be greater on d 34 and 50 than on d 16 and was greater ( = 0.02) on d 50 than on d 34. There was no effect of treatment for either or in CAR, ICAR, or FM. These data demonstrate that glucose transporters and are expressed in beef heifer uteroplacental tissues and that they are expressed differentially by day of gestation in bovine uteroplacental tissues.
Assuntos
Bovinos/genética , Proteínas Facilitadoras de Transporte de Glucose/genética , Prenhez , Animais , Sequência de Bases , Cruzamento , Bovinos/fisiologia , Endométrio/fisiologia , Sincronização do Estro , Feminino , Expressão Gênica , Proteínas Facilitadoras de Transporte de Glucose/metabolismo , Placenta/fisiologia , Gravidez , Fenômenos Fisiológicos da Nutrição Pré-Natal , Alinhamento de Sequência/veterinária , Útero/fisiologiaRESUMO
Endogenous retroviral gene elements have been implicated in development and formation of the feto-maternal interface. A variant of the syncytin endogenous retroviral envelope gene family, , was recently found in ruminants. We hypothesized that mRNA would be differentially expressed in utero-placental tissues and would fluctuate during key time points of early gestation in beef heifers. Commercial Angus crossbred heifers ( = 46; â¼15 mo of age; BW = 362.3 ± 34.7kg) housed in 6-animal pens were fed daily with native grass hay and supplemented with cracked corn to gain 0.3 kg/d. The heifers were estrus synchronized, artificially inseminated, (d of breeding= d 0) and ovariohysterectomized on d 16, 22, 28, 34, 40, and 50 ( = 9, 6, 6, 7, 6, and 5, respectively) of gestation and at d 16 of the estrous cycle for non-bred, non-pregnant controls (NP; = 7). Harvested tissues were separated into maternal caruncle (CAR), intercarunclar endometrium (ICAR), and fetal membranes, (FM; chorioallantois, d 22 and later). All tissues were obtained from the ipsilateral uterine horn to the CL. Statistical analyses were conducted via the GLM procedure of SAS. Maternal CAR expression of was greater ( = 0.003) on d 50 by 81.5-fold compared to NP controls. At d 50 expression of in CAR was 190.3-fold greater than ( < 0.0001) ICAR. Fetal membranes had greater ( < 0.002) expression of from d 22 until d 50 of gestation compared to maternal ICAR (d 16 not analyzed). Expression of in FM was greater ( < 0.004) than in CAR until d 40 of gestation. Therefore, we conclude that is differentially expressed in utero-placental tissues and may be involved in the establishment of pregnancy. The expression of in maternal tissues is completely novel and indicates unique functions of syncytin in ruminant pregnancy.
Assuntos
Bovinos/fisiologia , Suplementos Nutricionais , Produtos do Gene env/metabolismo , Proteínas da Gravidez/metabolismo , Animais , Cruzamento , Bovinos/genética , Retrovirus Endógenos , Ciclo Estral , Sincronização do Estro , Feminino , Produtos do Gene env/genética , Inseminação Artificial , Placenta/fisiologia , Folhas de Planta , Poaceae , Gravidez , Proteínas da Gravidez/genética , Carne Vermelha , Sementes , Zea maysRESUMO
We hypothesized that GnRH would increase pregnancy risk (PR) in a split-time AI program for cows in which estrus was not detected. A total of 1,236 suckled beef cows at 12 locations in 3 states (Colorado, Kansas, and North Dakota) were enrolled. Before applying the fixed-time AI program, BCS was assessed. Cows were treated on d -7 with a progesterone insert concurrent with 100 µg GnRH and on d 0 with 25 mg PGF plus removal of the insert. Estrus-detection patches were affixed to cows at insert removal. Estrus was defined to have occurred when an estrus-detection patch was >50% colored (activated). Cows in estrus by 65 h ( = 758; 61.3% of all cows) were randomly allocated to 2 treatments: 1) 100 µg GnRH and early + GnRH (E+G; = 373) or 2) AI only at 65 h (early - no GnRH [E-G]; = 385). The remaining cows were randomly allocated to 2 treatments: 1) 5(L+G; = 252) or 2) AI only at 84 h (late no GnRH [L-G]; = 226). Pregnancy was determined 35 d after AI via transrectal ultrasound. Pregnancy risk did not differ ( = 0.68) between E+G and E-G cows (61.9 vs. 60.4%, respectively). Conversely, for cows inseminated at 84 h, PR was greater ( = 0.01) in cows that received GnRH (L+G) compared with their herd mates not receiving GnRH (L- G; 41.7 vs. 30.8%, respectively). Of those cows not detected in estrus by 65 h, 42.1% were detected by 84 h, for a total expression of estrus by all cows of 77.6%. Administration of GnRH increased ( < 0.01) PR in cows not detected in estrus by 84 h (+GnRH = 33.4% [ = 146] vs. no GnRH = 15.0% [ = 128]) but had no effect in cows expressing estrus by 84 h (+GnRH = 65.3% [ = 103] vs. no GnRH = 61.7% [ = 97]). Neither estrus expression by 65 or 84 h nor PR was influenced by BCS, parity, or days postpartum at AI. Cows had greater PR when they had been detected in estrus before AI, and PR was improved by administration of GnRH at 65 h after insert removal in cows that were not detected in estrus and inseminated at 84 h.
Assuntos
Bovinos/fisiologia , Hormônio Liberador de Gonadotropina/administração & dosagem , Animais , Colorado , Estro , Detecção do Estro , Sincronização do Estro , Feminino , Inseminação Artificial/veterinária , Kansas , North Dakota , Paridade , Período Pós-Parto , Gravidez , Taxa de Gravidez , Progesterona/administração & dosagem , Distribuição Aleatória , RiscoRESUMO
During early gestation, nutrients are transported to the developing embryo via transporters in the uterine endometrium and chorioallantois. In the present study, we examined glucose transporters and and the cationic AA transporters , , and to test the hypotheses that 1) relative mRNA expression of transporters would be different among uteroplacental tissue type as gestation progresses and 2) concentrations of glucose and cationic AA would be different among target sites (placental compartments, serum, and histotrophic) and days of gestation. To test these hypotheses, crossbred Angus heifers ( = 46) were synchronized, bred via AI, and then ovariohysterectomized on d 16, 22, 28, 34, 40, or 50 of gestation (5 to 9/d) or not bred and ovariohysterectomized on d 16 of the synchronized estrous cycle ( = 7) to serve as nonpregnant (NP) controls. Uteroplacental tissues (maternal caruncle [CAR], intercaruncular endometrium [ICAR], and fetal membranes [FM; chorioallantois, d 22 and later]) were collected from the uterine horn ipsilateral to the corpus luteum immediately following ovariohysterectomy. Relative mRNA expression of the glucose transporters and cationic AA transporters was determined for each tissue from d 16 to 50 of gestation and from NP controls. Chorioallantoic, amniotic, and plasma fluids were collected from heifers on d 40 and 50 of gestation to determine concentrations of glucose and cationic AA. Expression of and showed a tendency ( < 0.10) toward being greater in d 16 ICAR and d 34 ICAR, respectively. Day × tissue interactions ( < 0.05) were present for , , and . Expression of was greater in d 50 CAR, expression of was greater on d 34 in ICAR, and expression of was greater in CAR tissue on d 34 compared with all other tissues and days of gestation. Glucose concentrations tended ( = 0.10) to be impacted by a day × fluid interaction. A day × fluid interaction ( = 0.01) for arginine concentration was observed, with greater concentrations in allantoic fluid on d 40 compared with all other days and fluid types. These data support our hypothesis that glucose and cationic AA transporters differ in their level of mRNA expression due to day of gestation and uteroplacental tissue type. In addition, concentrations of nutrients were differentially impacted by day, target site, and/or their respective interaction.
Assuntos
Aminoácidos/metabolismo , Bovinos/fisiologia , Glucose/metabolismo , Proteínas de Membrana Transportadoras/genética , Placenta/metabolismo , Útero/metabolismo , Animais , Transporte Biológico , Bovinos/embriologia , Endométrio/metabolismo , Ciclo Estral , Feminino , Alimentos , Regulação da Expressão Gênica no Desenvolvimento , Idade Gestacional , Proteínas de Membrana Transportadoras/metabolismo , GravidezRESUMO
We hypothesized that a standing flank ovariohysterectomy procedure could be developed in beef heifers that would provide high quality tissues for addressing critical questions during early pregnancy, while concomitantly keeping livestock stewardship a high priority. To test the hypothesis, we: 1) developed a standing flank ovariohysterectomy procedure for use in beef heifers, and 2) implemented this procedure in a cohort of heifers up to d 50 of pregnancy for tissue collections, documentation of post-surgical recovery, and assessment of feedlot finishing performance. Ovariectomy and cesarean section protocols are well established in research and veterinary medicine and were used as starting points for procedural development. Crossbred Angus heifers ( = 46; â¼ 15 mo of age; BW = 362.3 ± 34.7 kg) were used to develop this new surgical tissue collection technique. Heifers were subjected to the 5-d CO-Synch + CIDR estrous synchronization protocol so ovariohysterectomy occurred at d 16, 22, 28, 34, 40, and 50 of gestation. Key aspects of the standing flank ovariohysterectomy technique included 1) use of local anesthetic for a standing flank incision, 2) locate the uterine and ovarian arteries via blind palpation and ligate them through the broad ligament via an improved clinch knot, 3) cut the ovaries and uterus free from the broad ligament, 4) ligate the cervix and uterine branch of the vaginal artery, and 5) cut through the cervix and remove the reproductive tract. Surgical times, from skin incision to placement of the last suture, were influenced ( = 0.04) by stage of gestation. In pregnant heifers, time decreased from d 22 (120.0 ± 12.0 min) of gestation to d 40 (79.5 ± 12.0 min) of gestation; then increased at d 50 (90.5 ± 14.7 min) of gestation. Using this procedure, we obtained uterine, placental, and embryo/fetal tissues that had experienced limited hypoxia, little or no trauma, and thus were excellent quality for scientific study. All heifers recovered from surgery quickly and were moved to a finishing period. During the finishing period, ovariohysterectomized heifers had a DMI of 13.8 kg, gained 1.99 ± 0.35 kg/d, and had a G:F of 0.145 over 132-d. The standing flank ovariohysterectomy technique represents a new and viable model to economically obtain high quality tissues for investigating critical biological mechanisms during early pregnancy in beef heifers.
Assuntos
Bovinos/cirurgia , Histerectomia/veterinária , Ovariectomia/veterinária , Prenhez , Animais , Bovinos/fisiologia , Feminino , Histerectomia/métodos , Ovariectomia/métodos , Ovário , Gravidez , ProgesteronaRESUMO
Sixty-nine Angus-sired steer calves (332.3 kg initial BW) were used to determine the effects of single or double implant strategies on steers of high or low genetic potential (GP) determined by the GeneMax (Zoetis, Florham Park, NJ) genetic profiling test. Steers were assigned to treatments in a 2 × 2 factorial design with factors of 1) composite GP score (high, mean GP score of 86.5 [HI]; low, mean GP score of 25.3[LO]) and 2) implant strategy (single, steers implanted on d 70 [1X], or double, steers implanted d 0 and 70 [2X]). All steers were given the same implant (Revalor-S; Merck Animal Health, Summit, NJ), with the 2X group implanted on d 0 and 70 and the 1X group implanted only on d 70. A diet containing 1.38 Mcal NEg/kg DM was fed ad libitum, once daily. Ultrasound was used to measure body composition characteristics on d 0 and 70. Steers were harvested after 140 d on feed. At both the d-0 and d-70 ultrasound, HI steers had greater ( < 0.001) percent intramuscular fat (IMF) than LO steers, but no differences ( ≥ 0.24) were observed in LM area (LMA), rib fat thickness (RF), or rump fat thickness (RMFT). Steers in the 2X group had larger ( = 0.02) LMA and less ( = 0.03) IMF on d 70 than 1X steers and no differences ( ≥ 0.50) in RF or RMFT were observed. From d 0 to 70, HI steers had ADG, DMI, and G:F ( ≥ 0.60) similar to LO steers; however, 2X steers had greater ( < 0.001) ADG and were more ( < 0.001) feed efficient compared with 1X steers during the same interval. Over the entire 140-d feeding period, there were no differences ( ≥ 0.6) in BW, ADG, DMI, or G:F between GP groups; however, 2X steers had greater ( = 0.03) ADG compared with 1X steers and still had similar ( ≥ 0.12) DMI and G:F. Upon slaughter, marbling score tended to be impacted by a GP × implant interaction (499.9 ± 18.5, 545.6 ± 18.5, 487.1 ± 18.5, and 469.8 ± 18.5 for HI and 2X, HI and 1X, LO and 2X, and LO and 1X, respectively; = 0.06). No differences ( ≥ 0.7) were observed between GP groups for HCW, LMA, RF, KPH, or yield grade (YG). Steers in the 1X group had less ( = 0.003) RF than 2X steers but similar ( ≥ 0.14) HCW, marbling, LMA, KPH, and YG. A greater proportion ( = 0.03) of HI steers had choice carcasses (100 ± 0.0%) compared with LO steers (87.8 ± 3.9%). Results of this study indicate that the GP test used in the current study predicted differences in IMF, carcass marbling, and percent carcasses graded as choice.
Assuntos
Ração Animal/análise , Composição Corporal/efeitos dos fármacos , Bovinos/fisiologia , Estradiol/farmacologia , Acetato de Trembolona/análogos & derivados , Aumento de Peso/efeitos dos fármacos , Tecido Adiposo , Animais , Bovinos/genética , Dieta/veterinária , Relação Dose-Resposta a Droga , Combinação de Medicamentos , Estradiol/administração & dosagem , Acetato de Trembolona/administração & dosagem , Acetato de Trembolona/farmacologia , Aumento de Peso/genéticaRESUMO
This 24-year old black female presented with a chief complaint of this gingival mass. She states she first noticed the lesion about three months ago and it has gotten progressively larger. What is your diagnosis?
Assuntos
Hemorragia Gengival/diagnóstico , Doenças Mandibulares/diagnóstico , Adulto , Carcinoma de Células Escamosas/diagnóstico , Diagnóstico Diferencial , Feminino , Fibroma Ossificante/diagnóstico , Granuloma/diagnóstico , Humanos , GravidezRESUMO
Four cases of a rare odontogenic lesion are reported. In each of the 4 examples, the lesion manifested itself as a well-circumscribed unilocular radiolucency in the mandibular third molar region in an adult. The histopathologic features consisted of an encapsulated proliferation of odontogenic hard and soft tissues. The hard tissue component consisted of dentin deposited in a peripheral ringlike configuration that enclosed odontogenic epithelium resembling adenomatoid odontogenic tumor. Whether this process represents a neoplasm or an odontogenic hamartoma is an unresolved question. Treatment in each case consisted of curettage, and no recurrences were observed.
Assuntos
Neoplasias Mandibulares/patologia , Tumores Odontogênicos/patologia , Adulto , Diagnóstico Diferencial , Feminino , Hamartoma/diagnóstico , Humanos , Masculino , Doenças Mandibulares/diagnósticoRESUMO
A multiinstitutional study of macrognathia secondary to renal osteodystrophy in dialysis patients is presented. The nine cases reviewed reveal a variety of radiographic and histopathologic features, some of which resemble fibrous dysplasia and others suggestive of Paget's disease of bone. This article contains diagnostic criteria for differentiating renal osteodystrophy from similar fibro-osseous proliferations along with a discussion of the underlying cause and appropriate therapeutic interventions.
Assuntos
Distúrbio Mineral e Ósseo na Doença Renal Crônica/patologia , Doenças Maxilomandibulares/etiologia , Terapia de Substituição Renal/efeitos adversos , Adulto , Doenças Ósseas Metabólicas/etiologia , Doenças Ósseas Metabólicas/patologia , Distúrbio Mineral e Ósseo na Doença Renal Crônica/complicações , Distúrbio Mineral e Ósseo na Doença Renal Crônica/metabolismo , Diagnóstico Diferencial , Feminino , Humanos , Doenças Maxilomandibulares/metabolismo , Doenças Maxilomandibulares/patologia , Masculino , Pessoa de Meia-IdadeRESUMO
PURPOSE: This article compares the demographic features of the anterior midline maxillary odontogenic keratocyst with those in other regions. MATERIALS AND METHODS: The data on 18 cases of anterior midline maxillary odontogenic keratocyst were reviewed. RESULTS: Thirteen of the cases were in men (72.2%), and 16 of the patients (88.9%) were past the age of 60 years. The mean age of the patients was 69.9 years, which is much higher than for odontogenic keratocysts in other locations. The most common clinical diagnoses were periapical cyst and nasopalatine duct cyst. CONCLUSION: It is important to include odontogenic keratocyst in the differential diagnosis of anterior midline maxillary radiolucencies, especially when they occur in older individuals.
Assuntos
Doenças Maxilares/diagnóstico , Cistos Odontogênicos/diagnóstico , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Cavidade Nasal , Cisto Periodontal/diagnóstico , Fatores SexuaisRESUMO
An atypical presentation of squamous cell carcinoma of the mandibular gingiva is presented. This case, along with previously reported cases in the literature, demonstrate the need for thorough intraoral examination and investigation of any unexplained, persistent lesions. Emphasis is placed upon histopathologic examination of such lesions in order to arrive at a definitive diagnosis.
Assuntos
Carcinoma de Células Escamosas/patologia , Neoplasias Gengivais/patologia , Idoso , Diagnóstico Diferencial , Feminino , Humanos , MandíbulaAssuntos
Fibroma Ossificante/patologia , Neoplasias Maxilares/patologia , Criança , Humanos , MasculinoRESUMO
Five previously unreported cases of congenital epulis of the newborn are presented. All five cases were on the anterior maxillary alveolar ridge. Four were removed at 2 days of age and one at 7 weeks. Light microscopy demonstrated large eosinophilic granular cells within vascular fibrous connective tissue. Immunohistochemical studies revealed a positivity for vimentin and neuron specific enolase. Cytogenetic evaluation performed on one case was normal. Estrogen and progesterone receptors were absent in the one case so studied. Electron microscopy demonstrated tumor cells that were filled with autophagosomes. Cellular organelles were significantly reduced and inversely related to the number of cytoplasmic autophagosomes. Many of the autophagosomes contained collagen precursors. Poorly formed junctional complexes were seen. Occasional tumor cells demonstrated long processes that contained contractile microfilaments, pinocytosis, and areas of exocytosis. These studies suggest the tumor cells represent early mesodermal cells that express pericytic and myofibroblastic features that undergo cytoplasmic autophagocytosis.
Assuntos
Neoplasias Gengivais/congênito , Neoplasias Gengivais/patologia , Tumor de Células Granulares/congênito , Tumor de Células Granulares/patologia , Colágeno/análise , Feminino , Neoplasias Gengivais/química , Tumor de Células Granulares/química , Histocitoquímica , Humanos , Lactente , Recém-Nascido , Razão de MasculinidadeRESUMO
One of the most feared complications of neurofibromatosis type I (NF-I) is development of cancer, which is estimated to occur in about 5% of cases. The most common associated malignancy is the neurofibrosarcoma (NFS). HOwever, oral NFS in association with NF-I has rarely been reported. We report two cases of oral NFS arising in patients with NF-I. Both patients died of their tumors. Oral NFS arising in association with NF-I appears to have an extremely poor prognosis, as do these tumors at other sites of the body.
Assuntos
Neoplasias Mandibulares/etiologia , Neoplasias Maxilares/etiologia , Neurofibroma/etiologia , Neurofibromatose 1/complicações , Adulto , Idoso , Feminino , Humanos , Neoplasias Mandibulares/patologia , Neoplasias Maxilares/patologia , Neurofibroma/patologia , Prognóstico , Resultado do TratamentoRESUMO
A case of eccrine spiradenoma of the lip is presented. This benign lesion of the eccrine sweat glands must be considered in the differential diagnosis of solitary or multiple soft tissue lesions of the head and neck. Final diagnosis is dependent on histologic examination. Carcinomatous transformation has been reported in eight cases, especially in those tumors of long duration.