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1.
Mech Ageing Dev ; 156: 55-62, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27134149

RESUMO

The male Fischer 344 rat is an established model to study progressive renal dysfunction that is similar, but not identical, to chronic kidney disease (CKD) in humans. These studies were designed to assess age-dependent alterations in renal structure and function at late-life timepoints, 16-24 months. Elevations in BUN and plasma creatinine were not significant until 24 months, however, elevations in the more sensitive markers of function, plasma cystatin C and proteinuria, were detectable at 16 and 18 months, respectively. Interestingly, cystatin C levels were not corrected by caloric restriction. Urinary Kim-1, a marker of CKD, was elevated as early as 16 months. Klotho gene expression was significantly decreased at 24 months, but not at earlier timepoints. Alterations in renal structure, glomerulosclerosis and tubulointerstitial fibrosis, were noted at 16 months, with little change from 18 to 24 months. Tubulointerstitial inflammation was increased at 16 months, and remained similar from 18 to 24 months. A SEM (structural equation modeling) model of age-related renal dysfunction suggests that proteinuria is a marker of renal damage, while urinary Kim-1 is a marker of both damage and function. Taken together, these results demonstrate that age-dependent nephropathy begins as early as 16 months and progresses rapidly over the next 8 months.


Assuntos
Envelhecimento , Moléculas de Adesão Celular/urina , Cistatina C/sangue , Modelos Biológicos , Proteinúria , Insuficiência Renal Crônica , Envelhecimento/sangue , Envelhecimento/urina , Animais , Glucuronidase/metabolismo , Humanos , Proteínas Klotho , Masculino , Proteinúria/sangue , Proteinúria/urina , Ratos , Ratos Endogâmicos F344 , Insuficiência Renal Crônica/sangue , Insuficiência Renal Crônica/urina
2.
Am J Nephrol ; 39(5): 409-17, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24818804

RESUMO

BACKGROUND: The aging kidney has a decreased ability to repair following injury. We have shown a loss in expression of α-catenin in the aging rat kidney and hypothesize that decreased α-catenin expression in tubular epithelial cells results in diminished repair capacity. METHODS: In an effort to elucidate alterations due to the loss of α-catenin, we generated NRK-52E cell lines with stable knockdown of α(E)-catenin. RESULTS: α(E)-catenin knockdown resulted in decreased wound repair due to alterations in cell migration. Analysis of gene expression in the α(E)-catenin knockdown cells demonstrated almost a complete loss of bone morphogenetic protein-7 (BMP-7) expression that was associated with decreased phospho-Smad1/5/8 staining. However, addition of exogenous BMP-7 increased phospho-Smad1/5/8, suggesting that the BMP-7 pathway remained intact in C2 cells. Given the potential role of BMP-7 in repair, we investigated its role in wound repair. Inhibition of BMP-7 decreased repair in non-targeted control cells; conversely, exogenous BMP-7 restored repair in α(E)-catenin knockdown cells to control levels. CONCLUSIONS: Taken together, the data suggests that the loss of α(E)-catenin expression and subsequent downregulation of BMP-7 is a mechanism underlying the altered migration of tubular epithelial cells that contributes to the inability of the aging kidney to repair following injury.


Assuntos
Proteína Morfogenética Óssea 7/metabolismo , Células Epiteliais/fisiologia , Transdução de Sinais/genética , alfa Catenina/genética , alfa Catenina/metabolismo , Animais , Proteína Morfogenética Óssea 7/genética , Proteína Morfogenética Óssea 7/farmacologia , Linhagem Celular , Movimento Celular/genética , Proliferação de Células , Regulação para Baixo , Expressão Gênica , Regulação da Expressão Gênica , Técnicas de Silenciamento de Genes , Túbulos Renais , Masculino , Camundongos , Fosforilação , Transdução de Sinais/efeitos dos fármacos , Proteína Smad1/metabolismo , Proteína Smad5/metabolismo , Proteína Smad8/metabolismo , Cicatrização
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