Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
Microbiology (Reading) ; 145 ( Pt 9): 2453-2461, 1999 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10517598

RESUMO

The Bordetella bronchiseptica tonB gene was cloned by detection of a chromosomal restriction fragment hybridizing with each of two degenerate oligonucleotides that corresponded to Pro-Glu and Pro-Lys repeats characteristic of known TonB proteins. The tonB(Bb) gene was situated upstream of exbB and exbD homologues and downstream of a putative Fur-regulated promoter. Hybridization results indicated that the tonB operon and flanking regions were highly conserved between B. bronchiseptica, Bordetella pertussis and Bordetella parapertussis. Disruption of tonB in B. bronchiseptica resulted in inability to grow in iron-limiting media, and inability to utilize alcaligin, enterobactin, ferrichrome, desferroxamine B, haemin and haemoglobin. Although it was not possible to inactivate tonB in a clinical B. pertussis isolate, tonB was disrupted in a laboratory B. pertussis strain previously selected for the ability to grow on Luria-Bertani medium. This B. pertussis tonB mutant shared a similar iron complex utilization deficient phenotype with the B. bronchiseptica tonB mutant. The B. bronchiseptica tonB operon present on a plasmid did not complement an Escherichia coli tonB mutant, but inefficient reconstitution of enterobactin utilization was observed in one fepA mutant harbouring plasmid copies of the B. pertussis fepA homologue and tonB(Bb) operon.


Assuntos
Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Bordetella bronchiseptica/metabolismo , Bordetella pertussis/metabolismo , Proteínas de Escherichia coli , Ferro/metabolismo , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Antibacterianos/farmacologia , Southern Blotting , Bordetella bronchiseptica/efeitos dos fármacos , Bordetella bronchiseptica/genética , Bordetella pertussis/efeitos dos fármacos , Bordetella pertussis/genética , Conjugação Genética , Enterobactina/metabolismo , Ferricromo/análogos & derivados , Ferricromo/farmacologia , Deleção de Genes , Hemina/metabolismo , Hemoglobinas/metabolismo , Humanos , Dados de Sequência Molecular , Óperon , Análise de Sequência de DNA , Sideróforos/metabolismo , Transformação Bacteriana
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA