Ameliorative effects of betaine and ascorbic acid on endocrine and erythrocytic parameters of sexually-maturing female Japanese quails during the dry season.

This study investigated the ameliorative effects of betaine and ascorbic acid on some endocrine and erythrocytic parameters in female Japanese quails (Coturnix coturnix japonica) reared during the dry season. A total of 372 fourteen- day-old female quails sourced commercially was kept in cages for 56 days. After seven days acclimation, all birds were weighed and allotted by complete random design to four groups with 3 replicates per group. Every group having 93 quails, comprised of 31 birds per replicate. Experimental groups were birds fed: Control (basal); ascorbic acid (AA), at 200 mg/Kg; betaine (BET) at 2 g/kg and combination of AA (200 mg/Kg) + BET (2 g/kg) of diets. Daily dry-bulb temperature (DBT), relative humidity (RH) and temperature-humidity index (THI) measured at 08:00 h, 13:00 h and 17:00 h fluctuated widely and exceeded the zone of thermal comfort for Japanese quails. Serum levels of catalase (CAT), reduced glutathione (GSH), cortisol, sex hormones (luteinizing hormone, LH and estradiol) and erythrocyte parameters (packed cell volume, PCV; red blood count, RBC; haemoglobin concentration, Hb; mean corpuscular volume (MCV), hemoglobin (MCH) were obtained at 28, 49 and 70 days of age. In female quails, AA ± BET increased (P < 0.05) CAT and GSH, but decreased (P < 0.05) cortisol levels when compared with control values at varying ages. There were higher (P < 0.05) values of LH in quails fed dietary AA + BET (28 and 49 day-old) and estradiol in those which consumed either BET or AA + BET (28, 49 and 70 day-old) and AA (at 70 day-old). At 49 day-old, either BET or AA + BET increased (P < 0.05) RBC count, but lowered (P < 0.05) MCV and MCH. In conclusion, betaine and ascorbic acid supplementation improved activities of serum sex and stress hormones, and erythrocytic parameters of Japanese quails during the dry season.

Seasonal variations in the morphology of the seminal vesicles of the African straw-coloured fruit bat (Eidolon helvum).

The study investigated seasonal changes in the morphology of the seminal vesicles of Eidolon helvum in a typical African tropical environment. Seminal vesicles of forty-eight bats were examined during the early rainy, late rainy and peak dry seasons, using gross anatomical, histological and ultrastructural techniques. The interlobular connective tissue septa from the external capsule demarcated the glandular parenchyma into numerous lobules of tubulo-alveolar glands. A simple cuboidal epithelium of mono- or bi-nucleated cuboidal cells lined the glandular mucosa during the rainy season. This was replaced by a bistratified cuboidal epithelium, whose upper layer of cells showed evidence of degeneration, during the dry season. The PAS-positive secretory products appeared to be numerous during the rainy season, but few and clumped together during the dry season. The mean gross weight, interlobular septal thickness, acini diameter and epithelial height of the glands varied significantly with season. Ultrastructural features of the secretory cells during the rainy season showed well-developed cytoplasmic organelles, numerous electron lucid secretory vesicles and electron-dense granules. Secretory epithelial cells of the seminal vesicles are very active during the rainy season, while regeneration of degenerated epithelium in preparation for the next reproductive cycle occurs during the dry season.

Effects of betaine and ascorbic acid supplementation on serum gonadotropin, testicular histological analysis and sperm quality in male Japanese quails during the dry season.

The study investigated the effects of betaine and ascorbic acid supplementation on serum gonadotropin, testicular histological features and sperm quality in male Japanese quails reared during the dry season. Two hundred and forty 14 day-old male Japanese quails, obtained commercially and housed in cages, were assigned randomly to four dietary treatments for 56 days. Each treatment comprised of 3 replicates (20 birds per replicate). Birds in Control group were fed only basal diet, while those in AA group consumed supplemental ascorbic acid at (200 mg/kg); BET group, betaine (2 g/kg) and AA + BET, a combination of AA (200 mg/kg) and betaine (2 g/kg) in diets. Feeds and water were offered to all birds ad libitum. The prevailing environmental conditions during the study predominantly exceeded the zone of thermal comfort for Japanese quails with DBT ranging from 25.0 to 37.0 °C; RH, 48.0-92.0% and THI, 69.8-91.0. At 28, 49 and 70 day-old, serum concentrations of malondialdhyde (MDA), superoxide dismutase (SOD), gonadotropins [luteinizing hormone (LH) and follicle stimulating hormones (FSH)] and testicular histological analyses were evaluated. Sperm quality was assessed in 70 day - old quails as sperm motility (SM), vitality (SV), total abnormalities (STA) and reserve (SR). Supplemental AA + BET decreased (P < 0.05) MDA, but improved (P < 0.05) SOD in 28, 49 and 70 day-old quails. Serum LH and FSH activities were enhanced (P < 0.05) in birds fed AA ± BET diets, but those which consumed BET or AA + BET showed enhanced (P < 0.05) testicular histological architecture and spermatogenic activities. Birds fed AA ± BET showed improved SM, SV and SR (P < 0.05), but lower STA in 70 days old male quails. It is concluded that supplementation of betaine and ascorbic acid enhances male reproductive potentials in Japanese quails during the dry season.