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1.
Mol Immunol ; 111: 136-144, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31054407

RESUMO

Production of antimicrobial peptides cathelicidins, interferons and cytokines is an important feature in airway epithelial host defense. The innate immune response to alpha-herpesvirus infection at the sites of primary replication has not been fully studied. Thus, the aim of this study was to determine the expression of innate immune components, cathelicidins, IFNß, TNFα and TNF receptors (TNFRI and TNFRII) during acute infection and reactivation of bovine herpesvirus type 1 (BoHV-1) and 5 (BoHV-5) in the respiratory tract and lymphoid tissue of their natural host. We found that BoHV infection modulates mainly the expression of BMAP28, a key cathelicidin in cattle. It was downregulated by both viruses in retropharyngeal lymph nodes of acutely infected-calves, and it was accompanied by a lower expression of IFNß, TNFα and TNFRI. BoHV-5 showed a pronounced role in the downregulation of BMAP28, even in nasal mucosa and lung. However, during reactivation, BoHV-5 upregulated both BMAP28 and IFNß in retropharyngeal lymph nodes. Acute replication induced also TNFα mRNA and protein synthesis, and expression of TNFRI and II was positively regulated during both acute infection and reactivation, particularly in the trachea. Moreover, BMAP27 was detected during BoHV-1 reactivation suggesting a potential role at this stage. Thus, cathelicidins are implicated in alpha-herpesvirus infections of the bovine respiratory system and the response is distinct during BoHV-1 and BoHV-5 acute infection and reactivation. This demonstrates that these viruses modulate differentially the components of innate immune response, possibly influencing their pathogenesis. This study provides an initial pilot analysis of factors that might be implicated in alpha-herpesvirus infection of the bovine respiratory system.


Assuntos
Catelicidinas/imunologia , Doenças dos Bovinos/imunologia , Infecções por Herpesviridae/imunologia , Herpesvirus Bovino 1/imunologia , Interferon beta/imunologia , Fator de Necrose Tumoral alfa/imunologia , Animais , Bovinos , Citocinas/imunologia , Infecções por Herpesviridae/veterinária , Imunidade Inata/imunologia , Projetos Piloto , RNA Mensageiro/imunologia , Receptores do Fator de Necrose Tumoral/imunologia , Sistema Respiratório/imunologia , Sistema Respiratório/virologia , Regulação para Cima/imunologia
2.
Virus Genes ; 52(3): 372-8, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26995219

RESUMO

Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus, belonging to the Rhadinovirus genus, which is increasingly associated with various problems of the reproductive tract of cattle. In Argentina, analysis of BoHV-4 strains isolated from cervico-vaginal mucus of aborted cows revealed a high genetic divergence among strains, which could be classified in three different groups: Genotype 1 comprises Movar-like strains (European prototype), Genotype 2 includes DN599-like strains (American prototype) and Genotype 3 corresponds to a novel genotype group. Understanding the replication behavior in cell cultures and the molecular characteristics of this pathogen of cattle is critical for the rational design of in vitro experiments. The aim of this work was to quantitatively evaluate the replication properties of different Argentinean BoHV-4 strains and to characterize their phylogenetic relationships. Significant differences were evident among the virus titers of the different BoHV-4 isolates in vitro. The most conserved gene was the major capsid protein (ORF25). The glycoprotein B (gB), glycoprotein H (gH), and thymidine kinsase (TK) genes displayed both synonymous and non-synonymous substitutions, with the highest diversity observed for gB, which displayed amino acid substitutions in 24 out of the 178 positions examined. Strains 09/759, 12/512, and 07/568 presented a deletion encompassing amino acid position 27 to 35, whereas strains 07/435 and 09/227 had a deletion from position 28 to 35. Two strains, 07/435 and 09/227, also displayed the highest divergence compared to the other strains analyzed. This study provides information about the in vitro replication and behavior of nine field isolates of BoHV-4. These findings are relevant since available information on the in vitro growth characteristics of BoHV-4 strains is scarce. The results from this study may also be useful for establishing comparisons with other related viruses.


Assuntos
Herpesvirus Bovino 4/isolamento & purificação , Herpesvirus Bovino 4/fisiologia , Replicação Viral/genética , Animais , Argentina , Bovinos , Doenças dos Bovinos/virologia , Linhagem Celular , DNA Viral/genética , Feminino , Variação Genética , Genótipo , Infecções por Herpesviridae/veterinária , Infecções por Herpesviridae/virologia , Herpesvirus Bovino 4/genética , Filogenia , Timidina Quinase/genética , Vagina/virologia , Esfregaço Vaginal/veterinária , Proteínas do Envelope Viral/genética
3.
Res Vet Sci ; 96(1): 204-12, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24295740

RESUMO

Infection with Bovine Viral Diarrhea Viruses (BVDV) in cattle results in a wide range of clinical manifestations, ranging from mild respiratory disease to fetal death and mucosal disease, depending on the virulence of the virus and the immune and reproductive status of the host. In this study 30 Argentinean BVDV isolates were characterized by phylogenetic analysis. The isolates were genotyped based on comparison of the 5' untranslated region (5' UTR) and the E2 gene. In both phylogenetic trees, 76% of the viruses were assigned to BVDV 1b, whereas BVDV 1a, 2a and 2b were also found. Eight of the BVDV 1b isolates were further characterized by cross-neutralization tests using guinea pig antisera and sera from bovines vaccinated with two different commercial vaccines. The results demonstrated the presence of a marked antigenic diversity among Argentinean BVDV isolates and suggest the need to incorporate BVDV 1b isolates in diagnostic strategies.


Assuntos
Variação Antigênica/imunologia , Doença das Mucosas por Vírus da Diarreia Viral Bovina/imunologia , Vírus da Diarreia Viral Bovina/imunologia , Filogenia , Regiões 5' não Traduzidas/genética , Sequência de Aminoácidos , Animais , Variação Antigênica/genética , Argentina , Sequência de Bases , Doença das Mucosas por Vírus da Diarreia Viral Bovina/genética , Bovinos , Vírus da Diarreia Viral Bovina/genética , Cobaias , Dados de Sequência Molecular , Testes de Neutralização/veterinária , RNA Viral/química , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Alinhamento de Sequência , Análise de Sequência de DNA , Proteínas do Envelope Viral/química , Proteínas do Envelope Viral/genética
4.
Vet Parasitol ; 199(1-2): 1-7, 2014 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-24184173

RESUMO

The aim of this study was to evaluate whether Neospora caninum tachyzoites (Nc-1) inoculated by the conjunctival route in pregnant cows were able to generate infection in their fetuses. Group 1 contained 2 naturally infected cows; group 2 contained two cows inoculated intravenously with 2.5 × 10(8) tachyzoites, group 3 contained two cows inoculated with 2.5 × 10(8) tachyzoites by the conjunctival route, and group 4 contained two uninfected control cows. The four inoculated cows from groups 2 and 3 were challenged at 23 weeks of gestation. An indirect fluorescent antibody test (IFAT), recombinant NcGRA7-based ELISA, ELISA for IgG subisotypes and Western blot analysis were assessed to characterize the humoral immune response in dams. Sera from their fetuses were tested also using Western blot analysis. Routine microscopic evaluation of H&E stained fetal tissues was made and any fetal tissues and placentas with lesions compatible with Neospora-infection were processed by immunohistochemistry (IHC). DNA extraction from fresh and formalin-fixed, paraffin-embedded fetal tissues were tested by nested PCR. All dams from groups 1, 2 and 3 were seropositive by IFAT, rNcGRA7-based-ELISA and Western blot. IgG1/IgG2 ratios were ≤ 1 at weeks 27 and 29 of gestation. Only fetuses from groups 1 and 2 developed N. caninum specific antibodies by Western blot. Histopathological lesions compatible with those caused by N. caninum were observed in fetuses from groups 1 and 2. N. caninum cysts and tachyzoites were observed by IHC on fetal tissues from groups 1 and 2. Only fetal samples from group 2 were positive by PCR. Further work is needed not only to characterize the cellular immune response but also to clarify the consequences on the dam after conjunctival inoculation of N. caninum tachyzoites. This study shows that N. caninum tachyzoites inoculated by the conjunctival route were not vertically transmitted in pregnant cows.


Assuntos
Doenças dos Bovinos/transmissão , Coccidiose/veterinária , Neospora/fisiologia , Animais , Anticorpos Antiprotozoários/sangue , Western Blotting/veterinária , Bovinos , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/parasitologia , Doenças dos Bovinos/patologia , Coccidiose/imunologia , Coccidiose/patologia , Coccidiose/transmissão , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Feto/parasitologia , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Transmissão Vertical de Doenças Infecciosas/veterinária , Neospora/imunologia , Gravidez
5.
Vet Microbiol ; 160(1-2): 1-8, 2012 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-22673320

RESUMO

Bovine herpesvirus 4 (BoHV-4) is a γ-herpesvirus that has been isolated both from apparently healthy animals and from cattle with a variety of clinical signs, including post-partum endometritis and abortion. BoHV-4 causes either a persistent or a latent infection in cells of the monocyte/macrophage lineage. Two groups of BoVH-4 strains have been defined based on their restriction patterns: the Movar-like strains (European prototype) and the DN 599-like strains (American prototype). The purpose of the present study was to genetically characterize wild type BoHV-4 strains isolated from vaginal discharges of aborted cows in Argentina. The virus was identified by isolation and nested PCR in all vaginal discharge samples from aborted cows, either as a sole agent or in association with other pathogens. Restriction enzyme profiling and phylogenetic analysis demonstrated that there is a high genetic variability among the studied field isolates. The existence of three groups of strains, which were designated as genotypes 1, 2 and 3, is described. Genotypes 1 and 2 possibly correspond to the Movar-like and DN 599-like groups, respectively, whereas Genotype 3 corresponds to a novel group. Two viral strains did not cluster into any of these three groups, indicating that other genotypes could be circulating in Argentina. These results suggest a complex epidemiological background for the Argentinean BoHV-4 strains, probably influenced by independent events of genetic drift. This hypothesis cannot be rejected based on the available data. However, there is no direct evidence supporting this possibility. Thus, it seems speculative to suggest that interspecific jumps are responsible for the observed phylogenetic grouping. On the other hand, our analyses suggest a geographical structure for the observed viral genotypes, since genotypes 1 and 2 included the European (Movar-like) and American (DN599-like) reference strains, respectively. Geographic dispersion is known to be a driver of herpes viruses diversification, and independent evolution in geographical isolated places ensures the emergence of particular mutations in each location due to genetic drift (Compans, 2007; Zong et al., 1999). Therefore, at this point, the genetic drift hypothesis is the one that requires less ad-hoc considerations and thus, to our understanding, is the one that fits to the findings from this study. The involvement of this genetic variability in the detection and pathogenesis of BoHV-4 remains to be investigated.


Assuntos
Doenças dos Bovinos/virologia , Infecções por Herpesviridae/veterinária , Herpesvirus Bovino 4/genética , Aborto Animal/virologia , Animais , Argentina , Bovinos , Doenças dos Bovinos/genética , Linhagem Celular , Feminino , Infecções por Herpesviridae/genética , Infecções por Herpesviridae/virologia , Herpesvirus Bovino 4/isolamento & purificação , Filogenia , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterinária , Gravidez , Vagina/virologia , Esfregaço Vaginal/veterinária
6.
Rev. argent. microbiol ; Rev. argent. microbiol;41(2): 79-85, abr.-jun. 2009. graf, tab
Artigo em Inglês | LILACS | ID: lil-634620

RESUMO

The aim of this work was to study the in vitro amplification of BVDV (Pestivirus, Flaviridae) field isolates from Argentina in MDBK, BoTur and BHK-21 continuous cell lines. Field isolates 99/134 (mucosal disease), 00/693 (mucosal disease), 04P7016 (respiratory disease) and 04/89 (mucosal disease), genotype 1b, were used and compared with the Singer and NADL reference strains, genotype 1a. Additionally, cell lines derived from explants of bovine testis (RD- 420), bovine uterus (NCL-1) and porcine kidney (PKZ) were tested as alternative substrates for BVDV propagation in vitro. The effect of cell line, harvest time and infection protocol was evaluated. The viral titers observed depended on the virus and harvest time but not on the infection protocol. We found that MDBK and BoTur cell lines were susceptible to the infection whereas BHK-21 and PKZ were not. NADL viral titers, 00/693 and 04/89, increased from 24 to 48 h p.i. in BoTur cells and then reached a plateau, whereas those of 99/134 and 04P7016 remained constant between 24 and 72 h p.i. BVDV Singer, on the other hand, presented a maximum titer at 24 h p.i. and then decreased. BVDV-NADL titers increased in MDBK and NCL-1 but not in RD-420 between 24 and 48 h p.i., and then decreased at 72 h p.i. These facts lead us to conclude that neither the subgenotypes (1a, 1b) nor the clinical symptoms of the animal from the virus had been isolated seem to affect the virus cell line kinetics of viral replication in vitro. On the other hand, the most homogenous behavior, the most similar replication curves, and highest titers observed in MDBK and NCL-1 seem to indicate that these lines are generally more susceptible to BVDV replication.


Se estudió la interacción de aislamientos de campo de Argentina del VDVB (Pestivirus, Flaviridae) en las líneas celulares continuas MDBK, BoTur y BHK-21. Se utilizaron los virus de campo genotipo 1b, 99/134, 00/693 (casos compatibles con enfermedad de las mucosas) y 04P7016 (cuadro respiratorio) y las cepas de referencia genotipo 1a Singer y NADL. Además se evaluó la interacción de VDVB-NADL con las líneas celulares experimentales de bovino RD-420 y NCL-1 y de riñón porcino (PKZ). Se usaron 2 protocolos de infección. Los títulos virales observados dependieron del virus y del tiempo de infección y no así del modo de infección. Mientras que MDBK y BoTur resultaron susceptibles a la infección, BHK-21 y PKZ no lo fueron. Los virus NADL, 00/693 y 04/89 incrementaron su título entre las 24 y las 48 h p.i. en BoTur para mantenerlo posteriormente; los virus 99/134 y 04P7016 no presentaron variaciones y la cepa Singer presentó título máximo a las 24 h p.i para luego descender. La cinética del virus NADL en las células MDBK, RD-420 y NCL-1 tuvo un incremento de título para MDBK y NCL-1 entre las 24 y 48 h p.i que descendió a las 72 h p.i. La interacción virus-línea celular no estaría relacionada con el sub-genotipo del virus (1a o 1b), ni con el cuadro clínico; las células MDBK y NCL-1 serían más susceptibles a la replicación del VDVB.


Assuntos
Animais , Bovinos , Cricetinae , Cães , Feminino , Masculino , Doença das Mucosas por Vírus da Diarreia Viral Bovina/virologia , Vírus da Diarreia Viral Bovina/crescimento & desenvolvimento , Síndrome Hemorrágica Bovina/virologia , Técnicas In Vitro , Replicação Viral , Cultura de Vírus/métodos , Argentina/epidemiologia , Doença das Mucosas por Vírus da Diarreia Viral Bovina/epidemiologia , Técnicas de Cultura de Células/métodos , Linhagem Celular/virologia , Vírus da Diarreia Viral Bovina/isolamento & purificação , Síndrome Hemorrágica Bovina/epidemiologia , Rim/citologia , Mesocricetus , Especificidade de Órgãos , Suínos , Testículo/citologia , Útero/citologia
7.
Rev. argent. microbiol ; Rev. argent. microbiol;40(3): 151-157, jul.-sep. 2008. ilus, tab
Artigo em Inglês | LILACS | ID: lil-634593

RESUMO

A retrospective study was performed on 169 beef and dairy calves aged from 1 to 7 days old submitted to the Diagnostic Laboratories at INTA Balcarce, Argentina. Bacterial culture was performed for aerobic and microaerophilic organisms. Samples from spleen and lymph nodes, and peripheral blood mononuclear cells were also cultured for viral isolation on cell culture. Bovine rotavirus was detected by direct-ELISA. Multiple tissue samples were fixed in 10% formalin, routinely processed and stained with hematoxylin and eosin for microscopic examination. Etiological diagnosis was made in 70 of the 169 calves. Infectious agents were identified in 49 cases, the most common being Escherichia coli. When the histopathological examination was performed in cases with undetermined diagnosis, it was noted that 44 specimens had histological lesions, which suggested the presence of an infectious agent. In order to characterize the causes of bovine neonatal mortality, the protocols and methodology should be improved in further works.


Se realizó un estudio restrospectivo en 169 terneros muertos 1 a 7 días después del nacimiento pertenecientes a rodeos para carne y leche, remitidos a los Laboratorios de Diagnóstico del INTA Balcarce, Argentina. Para detectar organismos aeróbicos y microaerófilos se realizó el cultivo bacteriano. Para el aislamiento viral sobre cultivo celular, se recolectaron muestras de bazo, ganglios linfáticos y sangre periférica. El rotavirus bovino fue identificado por ELISA directo. Se efectuó el examen microscópico de diferentes tejidos, los cuales fueron fijados en formol al 10%, procesados y teñidos con hematoxilina y eosina. Se obtuvo un diagnóstico etiológico en 70 de los 169 terneros. Se identificaron agentes infecciosos en 49 casos, siendo el más común Escherichia coli. En los casos con diagnóstico indeterminado, el examen histopatológico realizado determinó que 44 especímenes poseían lesiones compatibles con la presencia de agentes infecciosos. Es necesario mejorar los protocolos y las metodologías de trabajo a los fines de caracterizar las causas de mortalidad neonatal en bovinos.


Assuntos
Animais , Bovinos , Feminino , Masculino , Animais Recém-Nascidos , Doenças dos Bovinos/mortalidade , Infecções/veterinária , Argentina , Doenças dos Bovinos/microbiologia , Infecções/mortalidade , Estudos Retrospectivos
8.
Rev. argent. microbiol ; Rev. argent. microbiol;37(4): 217-228, oct.-dic. 2005. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-634509

RESUMO

Neospora caninum es agente causal de aborto en bovinos de regiones ganaderas de todo el mundo. Su ciclo de vida es heteroxeno siendo el perro (Canis familiaris) y el coyote (Canis latrans) los hospedadores definitivos reconocidos hasta el presente. La infección transplacentaria es un eficiente mecanismo de transmisión de la enfermedad pero existe evidencia que demuestra la transmisión postnatal en los bovinos. Debido a las pérdidas económicas que causa la neosporosis, diversas técnicas diagnósticas han sido desarrolladas. La fisiopatología del aborto causado por N. caninum no ha sido completamente esclarecida. La modulación del sistema inmune por efecto de la preñez ocasiona un período de susceptibilidad al aborto por N. caninum. Aunque la resistencia al parásito ha sido asociada con una respuesta de linfocitos T tipo 1, dicha respuesta inmune es incompatible con una preñez exitosa. Sin embargo, los mecanismos inmunes presentes en animales crónicamente infectados protegen del aborto ante una segunda exposición al protozoo. La comprensión de esa respuesta inmune adquirida constituye un desafío para el desarrollo de inmunógenos. Este trabajo menciona conceptos generales de la neosporosis bovina haciendo énfasis en los mecanismos inmunes y las perspectivas para la vacunación.


Neospora caninum causes abortions in cattle worldwide. The Neospora-cycle of life is heteroxenous. Dogs (Canis familiaris) and coyotes (Canis latrans) are the definitive hosts known at present. Although, transplacental infection is an efficiently mode of transmission in cattle; there are also experimental and field data that prove horizontal transmission. Several techniques are available for diagnosis since neosporosis is recognized as a disease that causes economic losses in cattle. The mechanisms that produce the abortion are not completely understood. The immunomodulation observed during the pregnancy, is associated with a susceptible period where Neospora-abortion can occur. Resistance to the parasite is dependent on T helper cell 1 cytokine responses. This has important repercussions for pregnant female bovine because strong T helper cell 1 cytokine responses are incompatible with successful pregnancy. However, it was demonstrated that chronically infected cows develop immune mechanisms against the abortion caused by a second Neospora-exposure. The comprehension of those mechanisms is needed for the formulation of Neospora-vaccines that prevent bovine neosporosis. General concepts about neosporosis with emphasis in the immune response and perspectives for vaccination are mentioned in the present review.


Assuntos
Animais , Bovinos , Cães , Feminino , Gravidez , Aborto Animal/etiologia , Doenças dos Bovinos/parasitologia , Coccidiose/veterinária , Neospora , Complicações Infecciosas na Gravidez/veterinária , Aborto Animal/epidemiologia , Aborto Animal/imunologia , Aborto Animal/parasitologia , Aborto Animal/prevenção & controle , Anticorpos Antiprotozoários/imunologia , Argentina/epidemiologia , Coccidiostáticos , Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/prevenção & controle , Coccidiose/diagnóstico , Coccidiose/epidemiologia , Coccidiose/imunologia , Coccidiose/parasitologia , Coccidiose/prevenção & controle , Doenças do Cão/parasitologia , Doenças do Cão/transmissão , Imunidade Celular , Estágios do Ciclo de Vida , Neospora/crescimento & desenvolvimento , Neospora/imunologia , Neospora/isolamento & purificação , Complicações Infecciosas na Gravidez/epidemiologia , Complicações Infecciosas na Gravidez/parasitologia , Complicações Infecciosas na Gravidez/prevenção & controle , Vacinas Protozoárias/imunologia , Células Th1/imunologia , Vacinação/veterinária
9.
Rev. argent. microbiol ; Rev. argent. microbiol;35(2): 69-73, abr.-jun. 2003.
Artigo em Espanhol | LILACS | ID: lil-356632

RESUMO

A los fines de establecer la asociación entre herpesvirus bovino tipo 5 (HVB-5) y la necrosis cerebrocortical (NCC) se analizaron 89 casos bovinos de tres regiones de Buenos Aires registrados entre 1970-1999. El tejido nervioso fue teñido con hematoxilina-eosina o hibridización in situ para cADN-BHV-5. Las lesiones se clasificaron en una escala subjetiva de 0-3. La mayor prevalencia fue entre 1979-1984, principalmente en primavera, no existiendo diferencias (P>0,05) en las tasas de morbilidad y mortalidad entre grupos etarios, sexo, raza, sistemas de producción y regiones. Amaurosis (48 por ciento), ptialismo (42 por ciento), torneo (40 por ciento), ataxia (36 por ciento) y bruxismo (37 por ciento) fueron los síntomas más observados. Las lesiones estuvieron distribuidas en corteza anterior y posterior (90,6 por ciento) y diencéfalo (36,5 por ciento). Meningitis y manguitos perivasculares (94,4 por ciento) y gliosis difusa (77,5 por ciento) o focal (73 por ciento) fueron los hallazgos predominantes en el cerebro. Se observaron focos de necrosis en el 66,6 porciento de los casos. HVB-5 fue aislado en 9/19 casos a partir de 1992 y cADN-HVB-5 fue detectado en 3/9 casos. La identificación viral en casos con lesiones severas resultó negativa. La correspondencia de NCC con aislamiento viral de bovinos con similares características clínico-epidemiológicas permiten concluir la asociación del HVB-5 y lesiones de NCC.


Assuntos
Animais , Argentina , Encefalite , Infecções por Herpesviridae , Hibridização In Situ , Necrose
10.
Rev. argent. microbiol ; Rev. argent. microbiol;35(3): 143-8, 2003 Jul-Sep.
Artigo em Espanhol | LILACS-Express | LILACS, BINACIS | ID: biblio-1171723

RESUMO

The purpose of this study was to characterize the exposure of bovine aborted fetuses from beef and dairy herds of the humid pampas of Argentina to different infectious agents by the evaluation of fetal fluid antibodies. Presence of fetal antibodies to bovine viral diarrhea virus genotype 1 (BVDV-1), bovine herpes virus type 1 (BHV-1), Leptospira interrogans, Brucella abortus, and Neospora caninum was determined. Of the 95 fetuses processed, 66 came from 49 beef herds and 29 from 12 dairy herds. The average gestational age of the aborted fetuses was 7.1 months. Antibodies to the mentioned agents were detected in 65 of the 95 fetal fluids (68.4


). In addition, antibodies to more than one infectious agent were detected in 32 fetuses (33.7


), suggesting fetal exposure to multiple antigens during gestation. There were antibodies to BVDV-1, BHV-1, N. caninum and Leptospira interrogans in 43 (45.2


) and 5 (5.2


) specimens, respectively. Antibodies to B. abortus were not detected in any of the fetal fluids. The results of this study provide information on the determination of antibodies in fluids from bovine aborted fetuses exposed to different infectious agents in the region.

11.
Comp Immunol Microbiol Infect Dis ; 22(2): 145-62, 1999 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-10051184

RESUMO

To test the hypothesis that development of a BTV-specific IgE response plays a role in clinical disease manifestation, the humoral immune response of cattle to inactivated and virulent BTV was studied. Three calves received three sensitizing immunizations of inactivated BTV, 3 weeks apart. The BTV-sensitized animals, two non-sensitized BTV-seropositive and 4 BTV-seronegative control cattle. were challenge-exposed with BTV-11, UC8 strain. All cattle inoculated with inactivated BTV developed group-specific non-neutralizing and serotype-specific neutralizing antibodies. The development of post-challenge-exposure neutralizing antibody titers was inversely correlated with protective immunity. None of the BTV-challenged animals showed clinical disease. The levels of IgE were greatest in the sensitized calves after virus challenge in comparison with control groups. The sequential development, specificity and intensity of virus protein-specific humoral responses were evaluated using immunostaining. After challenge exposure of BTV-sensitized and non-sensitized cattle, total and IgE antibodies reacted consistently within BTV structural proteins VP2, VP5 and VP7. Although no correlation was found between clinical disease and IgE, results add support to the hypothesis that IgE may be involved in the pathogenesis of clinical disease, since infection with BTV causes an increase in serum IgE levels. However, these results suggest that the levels of virus-specific reactivity may be an important factor in determining whether or not clinical disease manifestation occurs.


Assuntos
Vírus Bluetongue/imunologia , Bluetongue/imunologia , Doenças dos Bovinos/imunologia , Imunoglobulina E/biossíntese , Vacinas Virais , Animais , Anticorpos Antivirais/biossíntese , Anticorpos Antivirais/sangue , Western Blotting/veterinária , Bovinos , Doenças dos Bovinos/virologia , Chlorocebus aethiops , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Imunodifusão/veterinária , Técnicas Imunoenzimáticas/veterinária , Imunoglobulina E/sangue , Testes de Neutralização/veterinária , Vacinação/veterinária , Vacinas de Produtos Inativados , Células Vero , Viremia/sangue
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