Comprehensive assessment of chlorination disinfection on microplastic-associated biofilms.

Chlorination on microplastic (MP) biofilms was comprehensively investigated with respect to disinfection efficiency, morphology, and core microbiome. The experiments were performed under various conditions: i) MP particles; polypropylene (PP) and polystyrene (PS), ii) MP biofilms; Escherichia coli for single-species and river water microorganisms for multiple-species, iii) different chlorine concentrations, and iv) different chlorine exposure periods. As a result, chlorination effectively inactivated the MP biofilm microorganisms. The disinfection efficiency increased with increasing the free chlorination concentration and exposure periods for both single- and multiple-species MP biofilms. The multiple-species MP biofilms were inactivated 1.3-6.0 times less than single-species MP biofilms. In addition, the PP-MP biofilms were more vulnerable to chlorination than the PS-MP biofilms. Morphology analysis verified that chlorination detached most MP biofilms, while a small part still remained. Interestingly, chlorination strongly changed the biofilm microbiome on MPs; the relative abundance of some microbes increased after the chlorination, suggesting they could be regarded as chlorine-resistant bacteria. Some potential pathogens were also remained on the MP particles after the chlorination. Notably, chlorination was effective in inactivating the MP biofilms. Further research should be performed to evaluate the impacts of residual MP biofilms on the environment.

Microplastics in aquatic ecosystems of Africa: A comprehensive review and meta-analysis.

Microplastic pollution is a global issue of great public concern. Africa is flagged to host some of the most polluted water bodies globally, but there is no enough information on the extent of microplastic contamination and the potential risks of microplastic pollution in African aquatic ecosystems. This meta-analysis has integrated data from published articles about microplastic pollution in African aquatic ecosystems. The data on the microplastic distribution and morphological characteristics in water, sediments and biota from African rivers, lakes, oceans and seas were extracted from 75 selected studies. Multivariate statistics were used to critically analyze the effects of sampling and detection methods, ecological risks, spatial distribution and similarity of microplastics in relation to the geographical distance between sampling sites. This study found that sampling methods have significant effect on abundance and morphological characteristics of microplastics and that African aquatic ecosystems are highly contaminated with microplastics compared to global data. The most prevalent colors were white, transparent and black, the most prevalent shapes were fibres and fragments, and the most available polymers were polypropylene (PP), polystyrene (PS) and polyethene terephthalate (PET). Microplastic polymers similarity decreased with an increase in geographical distance between sites. Risk levels of microplastics in African aquatic ecosystems were comparatively high, and more than 40 % of water and sediments showed highest level of ecological risk. This review provides recent information on the prevalence, distribution and risks of microplastics in African aquatic ecosystems.

Evolution and adaptation of SAR11 and Cyanobium in a saline Tibetan lake.

Lake Qinghai is a unique lacustrine ecosystem located on the Tibetan Plateau and exhibits oligotrophic, alkaline, and saline conditions. Previous studies have focused on the community phylogenetic diversity of bacterioplankton in the ecosystem. This study aimed to address the ecotype diversity of bacterioplankton populations in the unique microbial habitat, using metagenomic sequencing and analysis. Phylogenetic analysis revealed two major bacterial populations: SAR11 IIIa (14% of the total) and Cyanobium (14%). Although the two populations shared high 16S rRNA gene sequence identity (> 98% identity) with their closest marine counterparts, they displayed substantial genomic divergence (≤ 80% average amino acid sequence identity). Comparative genomic analysis identified conservation of carbon and energy storage metabolism (biosynthesis of polyphosphate and polyhydroxyalkanoate) gene operons in the SAR11 IIIa and a cyanate (potential nitrogen source in alkaline conditions) transporter gene operon in the Cyanobium. We further identified genetic signature of positive selection acting on an exodeoxyribonuclease gene of the SAR11 IIIa population, which is potentially associated with DNA repair responsive to strong UV radiation on the high altitude mountain. Taken together, our results revealed the ecosystem-specific gene content of the bacterioplankton populations and provided new insights into their adaptations unique to the Tibetan lake.