[Expression of p29, estrogen receptor related protein in primary breast carcinoma. Methodological, anatomoclinical, and DNA content analysis]. / Expresion de una proteina relacionada al receptor de estrogeno (p29), en carcinoma primario de la mama. Análisis metodológico, anatomoclínico y del contenido de ADN.

The aim of this work was to assess the immunohistochemical detection of a estrogen receptor related protein (p29) in 48 histological samples of primary mammary carcinoma and its relationship to clinical, morphological and ADN content parameters. p29 protein was positive in 62.5% of samples. Over 50% of samples had a moderate or intense immunohistochemical staining (staining index over 16) and 77% has a heterogeneous expression of p29 protein. Seventy six percent of p29 positive and 53% of p29 negative tumors had a proliferation fraction over 10% (determined by the S fraction with flux cytometry). No relationship between p29 expression and the analyzed anatomoclinical variables was found. These results highlight this immunohistochemical method as an alternative to more complex and difficult biochemical techniques. On the other hand, the good results obtained in formalin fixed tissues allow retrospective studies in mammary carcinoma samples.

[Breast cancer and flow cytometry: comparative study of DNA ploidy pattern with clinicopathological parameters]. / Cáncer de mama y citometría de flujo: estudio comparativo del patrón de ploidia de ADN con parámetros anatomoclnicos.

There is evidence that DNA quantization and histopathological classification of breast cancer may be useful for its therapeutic management. DNA flow cytometry, clinical and anatomopathological features of 60 paraffin embedded primary breast cancer tissue samples were studied. The aneuploidy percentage was 67%. There was a correlation between DNA index and degree of cellular pleomorphism, degree of differentiation and the fraction of cells in S phase. Likewise a correlation was found between the degree of cellular pleomorphism and the mitotic index. DNA cytophotometry was useful to solve cases of difficult diagnosis with flow cytometry ("near" diploid and tetraploid tumors). No correlation was found between aneuploidy, percentage of cells in phase S, degree of cellular atypia and mitotic degree with clinical stage, degree of lymph node involvement, tumoral size or age. It is suggested that these variables may have an independent behavior.

Fiebre tifoidea. Complicaciones en 782 ninos hospitalizados. / Typhoid fever.Complications in 782 hospitalized children

Se revisaron 782 casos de fiebre tifoidea en ninos hospitalizados a traves de un periodo de 5 anos en dos establecimientos de Santiago Los separamos en 2 grupos: a) con bacteriologia negativa. b) complicados o no complicados Se analizaron porcentaje, tipo, tratamiento y evolucion de los casos complicados. El resultado de nuestra revision indica que se complica el 12,7% de los casos. De ellos, solo el 5,4% corresponde a casos bacteriologicamente confirmados y el 7,3% a casos con bacteriologia negativa. Se uso cloramfenicol en la mayoria de los pacientes, solo excepcionalmente se recurrio a la cirugia; la evolucion de casi todos fue satisfactoria, excepto en caso en el que el paciente fallecio