RESUMO
Acute myeloid leukemia (AML) is the most common form of acute leukemia in adults and associated with poor prognosis. Unfortunately, most of the patients that achieve clinical complete remission after the treatment will ultimately relapse due to the persistence of minimal residual disease (MRD), that is not measurable using conventional technologies in the clinic. Microfluidics is a potential tool to improve the diagnosis by providing early detection of MRD. Herein, different designs of microfluidic devices were developed to promote lateral and vertical mixing of cells in microchannels to increase the contact area of the cells of interest with the inner surface of the device. Possible interactions between the cells and the surface were studied using fluid simulations. For the isolation of leukemic blasts, a positive selection strategy was used, targeting the cells of interest using a panel of specific biomarkers expressed in immature and aberrant blasts. Finally, once the optimisation was complete, the best conditions were used to process patient samples for downstream analysis and benchmarking, including phenotypic and genetic characterisation. The potential of these microfluidic devices to isolate and detect AML blasts may be exploited for the monitoring of AML patients at different stages of the disease.
Assuntos
Separação Celular , Leucemia Mieloide Aguda , Humanos , Leucemia Mieloide Aguda/patologia , Leucemia Mieloide Aguda/sangue , Separação Celular/métodos , Separação Celular/instrumentação , Dispositivos Lab-On-A-Chip , Técnicas Analíticas Microfluídicas/métodos , Técnicas Analíticas Microfluídicas/instrumentaçãoRESUMO
Objetivo:evaluar la asociación entre la dieta y factores socioeconómicos y demográficos entre los estudiantes de la escuela primaria.Métodos:Un estudio transseccional que involucra una muestra aleatoria de 256 niños de ambos sexos, de seis a once años, del primero a quinto grado de siete escuelas adventistas ubicados en la región suroeste de Sao Paulo. Estas escuelas inscriben estudiantes sin importar su religión. Datos demográficos y socioeconómicos se obtuvieron a partir de un cuestionario autoadministrado, llevado a casa y completado por el padre o responsable del niño. La calidad de la dieta se evaluó mediante el índice de ALES. Se calcularon las odds ratio brutas y ajustadas y se utilizó la regresión logística para el análisis de las variables.Resultados:De la muestra, 33,2% tuvo una puntuación pobre en el índice ALES y el 66,8% obtuvo una buena puntuación. No hubo diferencias significativas entre la calidad de la dieta y las siguientes variables: edad, nivel socioeconómico, educación maternal, jefe de la familia, trabajo materno fuera del hogar, lactancia materna exclusiva hasta los 6 meses, y el origen de las meriendas escolares. Las variables que se mantuvieron asociadas a una dieta de buena calidad fueron: ser del sexo femenino (OR = 0.53; 95% IC, 0.30-0.92) y ser Adventista (OR = 2.52; 95% IC, 1.10-5.78).Conclusión:Los factores asociados con la buena calidad de la dieta fueron el sexo y la religión.
Objective:to evaluate the association between diet and socioeconomic and demographic factors among elementary school students.Methods:A cross-sectional study, involving a random sample of 256 children of both genders, aged between six to eleven years, from grades first through fifth of seven Adventist elementary private schools located in the southwest region of São Paulo State. These schools enroll students independent of their religion. Socioeconomic and demographic data were obtained through a self-administered questionnaire sent home and completed by the parent or legal guardian. The diet quality was assessed through ALES index. Crude and adjusted odds ratios were calculated and logistic regression was used to analyze the variables.Results:Of the sample, 33.2% showed a poor ALES index score and 66.8% had a good score. There were no statistically significant differences between diet quality and the following variables: age, socioeconomic status, maternal level of education, head of household, maternal employment outside the home, exclusive breastfeeding up to 6 months, and origin of school snacks. The variables that remained associated with a good diet quality were: being female (OR = 0.53; 95% CI, 0.30-0.92) and being Adventist (OR = 2.52; 95% CI, 1.10-5.78).Conclusions:The factors associated with good diet quality were gender and religion.