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2.
Gan To Kagaku Ryoho ; 24(15): 2281-4, 1997 Dec.
Artigo em Japonês | MEDLINE | ID: mdl-9422074

RESUMO

The patient was a 83-year-old female with 2' T2-type gastric cancer associated with positive H. pylori in the lesser curvature of the stomach. The patient was treated with oral UFT-E alone in a daily dose of 400 mg. The tumor exhibited an O' IIa + IIc-like appearance 4 weeks after the start of administration and became scarred 8 weeks later, revealing marked tumor reduction in a short period of time. At 8 weeks, biopsy showed marked polymorphonuclear cells infiltration of gastric mucosa with no evidence of malignancy. In an attempt to eradicate H. pylori, 30 mg of lansoprazole, 400 mg of clarithromycin, and 2.0 g of ecabet-Na (3.0 g of Gastrom) were administered for 2 weeks. H. pylori was found to have been successfully eradicated, and the inflammatory lesions were no longer visible histologically. UFT-E was highly effective in this patient, and the eradication of H. pylori may contribute to the prevention of cancer recurrence.


Assuntos
Abietanos , Adenocarcinoma/tratamento farmacológico , Antibacterianos/administração & dosagem , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori , Neoplasias Gástricas/tratamento farmacológico , 2-Piridinilmetilsulfinilbenzimidazóis , Adenocarcinoma/microbiologia , Idoso , Idoso de 80 Anos ou mais , Claritromicina/administração & dosagem , Diterpenos/administração & dosagem , Feminino , Helicobacter pylori/isolamento & purificação , Humanos , Lansoprazol , Omeprazol/administração & dosagem , Omeprazol/análogos & derivados , Neoplasias Gástricas/microbiologia , Tegafur/administração & dosagem , Uracila/administração & dosagem
3.
Acta Biochim Pol ; 41(3): 281-92, 1994.
Artigo em Inglês | MEDLINE | ID: mdl-7856399

RESUMO

The gene for thermostable farnesyl diphosphate synthase from Bacillus stearothermophilus was cloned, sequenced, and overexpressed in Escherichia coli. The synthase was purified to homogeneity and crystallized. The enzyme carried only two cysteine residues in contrast to its counterparts from other sources, which have four to six cysteine residues. Either or both of the cysteine residues can be replaced with serine without causing a loss of the catalytic activity. The conserved arginine residue that occupies the third position from the C-terminus was also replaced with valine without significant loss of activity, but the valine mutant showed a weakened affinity for isopentenyl diphosphate.


Assuntos
Dimetilaliltranstransferase/metabolismo , Geobacillus stearothermophilus/enzimologia , Sequência de Aminoácidos , Arginina , Sequência de Bases , Clonagem Molecular , Sequência Conservada , Cristalização , Dimetilaliltranstransferase/química , Dimetilaliltranstransferase/isolamento & purificação , Eletroforese em Gel de Poliacrilamida , Estabilidade Enzimática , Fungos/enzimologia , Temperatura Alta , Humanos , Cinética , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Oligodesoxirribonucleotídeos , Plasmídeos , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Mapeamento por Restrição , Homologia de Sequência de Aminoácidos , Termodinâmica
4.
J Biochem ; 113(3): 355-63, 1993 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-8486607

RESUMO

The structural gene for thermostable farnesyl diphosphate synthase from Bacillus stearothermophilus was cloned, sequenced, and overexpressed in Escherichia coli cells. A 1,260-nucleotide sequence of the cloned fragment was determined. This sequence specifies an open reading frame of 891 nucleotides for farnesyl diphosphate synthase. The deduced amino acid sequence shows a 42% similarity with that of E. coli FPP synthase [Fujisaki et al. (1990) J. Biochem. 108, 995-1000]. Comparison with prenyltransferases from a wide range of organisms, from bacteria to human, revealed the presence of seven highly conserved regions. In contrast to thermolabile prenyltransferases, which have four to six cysteine residues, the thermostable farnesyl diphosphate synthase carries only two cysteine residues. This enzyme is also unique in that some of the amino acids that are fully conserved in equivalents from other sources are replaced by functionally different amino acids. Construction of an overproducing strain provided a sufficient supply of this enzyme and it was purified to homogeneity. The purified recombinant enzyme is immunochemically identical with the native B. stearothermophilus enzyme, and it is not inactivated even after treatment at 65 degrees C for 70 min.


Assuntos
Alquil e Aril Transferases , Geobacillus stearothermophilus/enzimologia , Transferases/química , Sequência de Aminoácidos , Sequência de Bases , Cromatografia em Camada Fina , Clonagem Molecular , DNA Bacteriano/química , DNA Bacteriano/genética , Eletroforese em Gel de Poliacrilamida , Escherichia coli/enzimologia , Geraniltranstransferase , Dados de Sequência Molecular , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Mapeamento por Restrição , Temperatura , Transferases/biossíntese , Transferases/genética , Transferases/isolamento & purificação
7.
Jpn J Cancer Res ; 78(4): 382-5, 1987 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-3108218

RESUMO

N-Acetyl-S-butyl-L-cysteine, N-acetyl-S-3-oxobutyl-L-cysteine and N-acetyl-S-3-hydroxybutyl-L-cysteine have been isolated and identified (as their methyl esters) from the urine of rats given N-nitrosodibutylamine (NDBA), N-nitrodibutylamine (NO2DBA) and their 1-acetoxy derivatives. Greater amounts of these N-acetyl-S-alkyl-L-cysteines were detected in the urine after administration of NDBA than of NO2DBA, and greater urinary excretion of the three N-acetyl-S-alkyl-L-cysteines was observed with 1-acetoxy NDBA than with 1-acetoxy NO2DBA. This suggests that the markedly different biological activities of NDBA and NO2DBA might be due, in part, to a difference in their alkylating abilities in vivo.


Assuntos
Butilaminas/metabolismo , Cisteína/análogos & derivados , Nitrosaminas/metabolismo , Alquilação , Animais , Butilaminas/urina , Cisteína/urina , Glutationa/metabolismo , Mutagênicos/metabolismo , Nitrosaminas/urina , Ratos
8.
IARC Sci Publ ; (84): 153-5, 1987.
Artigo em Inglês | MEDLINE | ID: mdl-3679356

RESUMO

N-Acetyl-S-(butyl, 3-oxobutyl and 3-hydroxybutyl)-L-cysteines have been isolated and identified (as their methyl esters) from the urine of rats given N-nitrosodibutylamine (NDBA), N-nitrodibutylamine (NTDBA) and their corresponding alpha-acetoxy derivatives, N-nitroso-N-butyl(1-acetoxybutyl)amine and N-nitro-N-butyl(1-acetoxybutyl)amine, respectively. Greater amounts of these L-cysteine derivatives were detected in urine after administration of NDBA than of NTDBA. This suggests that the markedly different biological activities of NDBA and NTDBA might be due, in part, to a difference in their alkylating abilities in vivo.


Assuntos
Acetilcisteína/urina , Nitrosaminas/farmacocinética , Alquilação , Animais , Butilaminas/farmacocinética , Ratos
9.
IARC Sci Publ ; (84): 100-3, 1987.
Artigo em Inglês | MEDLINE | ID: mdl-3315995

RESUMO

Directly-acting mutagens formed from N-nitroso-N-(formylmethyl)alkylamines (I) were isolated and identified as N-nitroso-N-alkyl-1-hydroxyimino-2-oxoethylamines (II). Their structures were elucidated on the basis of nuclear magnetic resonance spectra and confirmed by leading to their crystalline 2,4-dinitrophenylhydrazone. II (alkyl = ethyl and n-butyl) were strongly mutagenic to Salmonella typhimurium TA1535 and Escherichia coli WP2 hcr- without metabolic activation, while II with a tert-butyl group was not mutagenic. The formation of II from I is considered to proceed by the nitrosation of I, indicating a possible involvement of a formylmethyl metabolite in the carcinogenesis of nitrosamines with a 2-hydroxyethyl group.


Assuntos
Mutagênicos/isolamento & purificação , Nitrosaminas/metabolismo , Animais , Escherichia coli , Espectroscopia de Ressonância Magnética , Testes de Mutagenicidade , Ratos , Salmonella typhimurium , Relação Estrutura-Atividade
10.
Jpn J Cancer Res ; 76(1): 28-36, 1985 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-3918908

RESUMO

The in vitro metabolism of N-nitramines was investigated in order to compare it with that of N-nitrosamines and to elucidate the mode of mutagenic action. N-Nitrodibutylamine (NO2DBA) and N-nitrodiethylamine (NO2DEA) were incubated with liver microsomes and hepatocytes prepared from rats treated with phenobarbital, and the products were analyzed by high-performance liquid chromatography and gas-liquid chromatography. The in vitro metabolic pattern of these nitramines was similar to that of the corresponding nitrosamines, except that N-nitro-N-alkylamines (produced via alpha-hydroxylation) were identified after incubation of the nitrodialkylamines. In the case of NO2DBA, besides N-nitro-N-butylamine, several nitramines produced by omega, omega-1, and omega-2 oxidations were identified as metabolites. NO2DBA and NO2DEA were mutagenic to Escherichia coli WP2 hcr- but not to Salmonella typhimurium TA1535. They were mutagenic only in the presence of hepatic microsomes, whereas their metabolites, N-nitro-N-butylamine and N-nitro-N-ethylamine, were direct mutagens. Thus, N-nitrodialkylamines are also metabolically activated to mutagens through alpha-hydroxylation.


Assuntos
Compostos de Anilina/metabolismo , Fígado/metabolismo , Mutagênicos/metabolismo , Nitrobenzenos/metabolismo , Animais , Dietilaminas/metabolismo , Técnicas In Vitro , Masculino , Microssomos Hepáticos/metabolismo , Oxirredução , Ratos , Ratos Endogâmicos , Salmonella typhimurium/efeitos dos fármacos
11.
J Cancer Res Clin Oncol ; 108(3): 290-5, 1984.
Artigo em Inglês | MEDLINE | ID: mdl-6511803

RESUMO

Carcinogenic and mutagenic N-nitrosodialkylamines are metabolically activated through alpha-hydroxylation. The synthesis, chemical properties, and microbial mutagenicity of alpha-hydroxy N-nitrosamines have been reported previously. Potent mutagenicity of four N-nitroso-N-(hydroxymethyl)-alkylamines (alkyl = methyl, ethyl, propyl, and butyl) was demonstrated in the present study in V79 Chinese hamster cells, ouabain resistance being used as an indicator. All the compounds were strong mutagens in the absence of metabolic activation systems. The mutagenic and cytotoxic potencies correlated well with each other, and depended on the alkyl group, decreasing in potency in the following order: methyl greater than ethyl greater than propyl = butyl. Their alkylating reactivity was measured by alkylation of thiophenol, and a good linear relationship was observed between the mutagenic and cytotoxic potencies and their alkylating reactivity. The mutagenic and cytotoxic potencies of the alpha-hydroxy N-nitrosamines in V79 cells were well correlated with those of alpha-acetoxy and alpha-hydroperoxy N-nitrosamines with respect to the effect of alkyl group. The results obtained here supported further that alpha-hydroxy N-nitrosamine is the active species in the metabolic activation of N-nitrosodialkylamine.


Assuntos
Mutagênicos , Nitrosaminas/toxicidade , Compostos de Sulfidrila , Alquilantes , Animais , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Cricetinae , Cricetulus , Concentração de Íons de Hidrogênio , Hidroxilação , Testes de Mutagenicidade/métodos , Fenóis , Relação Estrutura-Atividade
12.
IARC Sci Publ ; (57): 715-9, 1984.
Artigo em Inglês | MEDLINE | ID: mdl-6398304

RESUMO

N-Nitrosodialkylamines are activated metabolically by alpha-hydroxylation. Chemical properties and bacterial mutagenicity of alpha-hydroxy N-nitrosamines have been reported previously. This paper describes potent and direct mutagenicity of four N-nitroso-N-(hydroxymethyl)alkylamines in V79 Chinese hamster cells, using ouabain resistance as an indicator. The mutagenic potency depended on the alkyl group, decreasing in the following order: methyl greater than ethyl greater than propyl, butyl. A similar order was observed for cytotoxicity. Mutagenic and cytotoxic potencies of these alpha-hydroxy N-nitrosamines in V79 cells were well correlated not only with those of model compounds (alpha-acetoxy and alpha-hydroperoxy N-nitrosamines), but also with their alkylating ability, measured by alkylation of thiophenol. The mutagenic activity of the alpha-hydroxy N-nitrosamines in V79 cells was shown to be parallel to that in Salmonella typhimurium TA1535 and to that of N-nitrosodialkylamines in V79 cells, after metabolic activation by rat hepatocytes. The results obtained here further support the conclusion that the alpha-hydroxy N-nitrosamine is the active species in the metabolic activation of carcinogenic and mutagenic N-nitrosodialkylamines.


Assuntos
Mutagênicos , Nitrosaminas/farmacologia , Animais , Linhagem Celular , Cricetinae , Cricetulus , Testes de Mutagenicidade , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/genética
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