Dissolved organic nitrogen removal and its mechanisms during simulated soil aquifer treatment.

Soil aquifer treatment systems are known to further remove contaminants in wastewater effluent when applied through infiltration into the ground. Dissolved organic nitrogen (DON) in the effluent, a precursor for nitrogenous disinfection by-products (DBPs) such as N-nitrosodimethylamine (NDMA), is of great concern upon subsequent use of the groundwater infiltered into the aquifer. In this study, the vadose zone of the soil aquifer treatment system was simulated using 1 m laboratory soil columns under unsaturated conditions representing the vadose zone. The final effluent of a water reclamation facility (WRF) was applied to these columns to investigate the removal of N species with a focus on DON, as well as NDMA precursors. DON removal achieved was up to 99 % with an average of 68 % and was accompanied by a 52 % nitrate increase suggesting the occurrence of ammonification and nitrification through the soil columns. Around 62 % of total DON removal was seen at <10 cm travel distance, which was in accordance with higher adenosine triphosphate (ATP) concentrations at the top of the column due to more oxygen and organic matter availability. Total Dissolved N removal was drastically lowered to 4.5 % in the same column without microbial growth, which highlights the importance of biodegradation. The columns were capable of removing 56 % of the fluorescent dissolved organic matter (FDOM). Soil columns could remove NDMA precursors up to 92 % through the column with the initial concentration of 89.5 ng/L, possibly due to the removal of DON fractions. The results demonstrate the capability of the vadose zone in further treatment of DON and other organic matter before reaching the groundwater through infiltration or indirect discharge to surface water. Differences in applied water quality and the site-specific oxic conditions in SAT systems could lead to variable removal efficiencies.

Protozoa reduction through secondary wastewater treatment in two water reclamation facilities.

The State of Nevada, USA Administrative Code requires a 12-log enteric virus reduction/inactivation, 10-log Giardia cyst reduction, and 10-log Cryptosporidium oocyst reduction for Category A+ reclaimed water suitable for indirect potable reuse (IPR) based on raw wastewater to potable reuse water. Accurately demonstrating log10 reduction values (LRVs) through secondary biological treatment prior to an advanced water treatment train enables redundancy and resiliency for IPR projects while maintaining a high level of public confidence. LRVs for Cryptosporidium and Giardia resulting from secondary biological treatment are not fully established due to a wide range of performance variabilities resulting from different types of secondary biological treatment processes employed in water reclamation. A one-year investigation of two full-scale northern Nevada (e.g. ≤4 mgd; 1.5 × 107 L/day) water reclamation facilities (WRFs) was conducted to monitor Cryptosporidium oocysts and Giardia cysts in untreated wastewater and secondary effluent. This study aimed at establishing secondary treatment LRVs, monitor WRF performance and attempted to correlate performance to protozoan reduction. California's IPR regulations, in which Nevada IPR regulations were modeled after, were based on a maximum concentration of 5-logs (cysts/L) of Giardia and 4-logs (oocysts/L) of Cryptosporidium. The recovery-corrected Giardia and Cryptosporidium concentrations measured in untreated influent (20 samples each at each WRF) were below 5-log cysts/L at the 99th percentile (maximum 4.4-log cysts/L) and 4-log oocysts/L (maximum 2.7 log oocysts/L), respectively. Both secondary treatment WRFs produced secondary effluent that is consistently better than federal and the State of Nevada requirements and perform within an operating envelop for other secondary facilities. Given the results, it appears that a minimum conservative estimate for LRVs for well-operated secondary activated sludge treatment plants (at the 5th percentile) of 0.5 LRV credit for Cryptosporidium and 2.0 LRV for Giardia is warranted. These minimum LRVs are consistent with a conservative review of the available literature.

Fate and reduction of bromate formed in advanced water treatment ozonation systems: A critical review.

Disinfection in water treatment and reclamation systems eliminates the potential health risks associated with waterborne pathogens, however it may produce disinfection by-products (DBPs) harmful to human health. Potentially carcinogenic bromate is a DBP formed during the ozonation of bromide-containing waters. To mitigate the problem of bromate formation, different physical/chemical or biological reduction methods of bromate have been investigated. Until now, adsorption-based physical method has proven to be more effective than chemical methods in potable water treatment. Though several studies on biological reduction methods have been carried out in a variety of bioreactor systems, such as in biologically active carbon filters and denitrifying bioreactors, the microbiological mechanisms or biochemical pathways of bromate minimization have not been clearly determined to date. Genetic analysis could provide a broader picture of microorganisms involved in bromate reduction which might show cometabolic or respiratory pathways, and affirm the synergy functions between different contributing groups. The hypothesis established from the diffusion coefficients of different electron donor and acceptors, illustrates that some microorganisms preferring bromate over oxygen contain specific enzymes which lower the activation energy required for bromate reduction. In addition, considering microbial bromate reduction as an effective treatment strategy; field scale investigations are required to observe quantitative correlations of various influencing parameters such as pH, ozone dose, additives or constituents such as ammonia, hydrogen peroxide, and/or chloramine, dissolved organic carbon levels, dissolved oxygen gradient within biofilm, and empty bed contact time on bromate removal or reduction.

Density-Based Separation of Microbial Functional Groups in Activated Sludge.

Mechanistic understanding of how activated sludge (AS) solids density influences wastewater treatment processing is limited. Because microbial groups often generate and store intracellular inclusions during certain metabolic processes, it is hypothesized that some microorganisms, like polyphosphate-accumulating organisms (PAOs), would have higher biomass densities. The present study developed a density-based separation approach and applied it to suspended growth AS in two full-scale domestic water resource recovery facilities (WRRFs). Incorporating quantitative real-time PCR (qPCR) and fluorescence in situ hybridization (FISH) analyses, the research demonstrated the effectiveness of density-based separation in enriching key microbial functional groups, including ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and PAOs, by up to 90-fold in target biomass fractions. It was observed that WRRF process functionalities have significant influence on density-based enrichment, such that maximum enrichments were achieved in the sludge fraction denser than 1.036 g/cm3 for the enhanced biological phosphorus removal (EBPR) facility and in the sludge fraction lighter than 1.030 g/cm3 for the non-EBPR facility. Our results provide important information on the relationship between biomass density and enrichment of microbial functional groups in AS, contributing to future designs of enhanced biological treatment processes for improved AS settleability and performance.