Photobiomodulation and Sida tuberculata combination declines the inflammation's markers in knee-induced osteoarthritis.

The aim of this study was to assess potential combination effects of photobiomodulation therapy (PBMT) with Sida tuberculata extracts on the oxidative stress and antioxidant activity, as well as on the inflammatory process. Rats with knee osteoarthritis (OA) were treated with S. tuberculata extracts and PBMT (904 nm, 18 J/cm2). The animals were evaluated for nociception and edema. The blood, knee lavage and structures, spinal cord, and brainstem were collected for biochemical analyses (lipid peroxidation, protein carbonyl content, superoxide dismutase activity, non-protein thiol levels, and measurement of nitrite/nitrate). The knee structures were also used to measure cytokine levels. PBMT lowered the damage due to oxidative stress in the knee and at distant sites from the lesion. PBMT also reduced the levels of nitric oxide and cytokines, which could explain the nociception reduction mechanism. Similarly, S. tuberculata decreased the damage by oxidative stress, levels of nitrite/nitrate, and cytokines. The therapy combination reduced levels of cytokines and nitrite/nitrate. PBMT and S. tuberculata extracts reduced the oxidative stress and inflammation. It is noteworthy that PBMT increased the antioxidant activity in the knee and at sites distant from the lesion, contributing to a more significant decrease in nociception. The combination of therapies did not present significant effects on the analyzed parameters. Therefore, it is suggested that PBM is sufficient to minimize the signs and symptoms of the knee OA in our rat model.

Lipoic Acid and Fish Oil Combination Potentiates Neuroinflammation and Oxidative Stress Regulation and Prevents Cognitive Decline of Rats After Sepsis.

Sepsis causes organ dysfunction due to an infection, and it may impact the central nervous system. Neuroinflammation and oxidative stress are related to brain dysfunction after sepsis. Both processes affect microglia activation, neurotrophin production, and long-term cognition. Fish oil (FO) is an anti-inflammatory compound, and lipoic acid (LA) is a universal antioxidant substance. They exert neuroprotective roles when administered alone. We aimed at determining the effect of FO+LA combination on microglia activation and brain dysfunction after sepsis. Microglia cells from neonatal pups were co-treated with lipopolysaccharide (LPS) and FO or LA, alone or combined, for 24 h. Cytokine levels were measured. Wistar rats were subjected to sepsis by cecal ligation and perforation (CLP) and treated orally with FO, LA, or FO+LA. At 24 h after surgery, the hippocampus, prefrontal cortex, and total cortex were obtained and assayed for levels of cytokines, myeloperoxidase (MPO) activity, protein carbonyls, superoxide dismutase (SOD), and catalase (CAT) activity. At 10 days after surgery, brain-derived neurotrophic factor (BDNF) levels were determined and behavioral tests were performed. The combination diminished in vitro levels of pro-inflammatory cytokines. The combination reduced TNF-α in the cortex, IL-1ß in the prefrontal cortex, as well as MPO activity, and decreased protein carbonyls formation in all structures. The combination enhanced catalase activity in the prefrontal cortex and hippocampus, elevated BDNF levels in all structures, and prevented behavioral impairment. In summary, the combination was effective in preventing cognitive damage by reducing neuroinflammation and oxidative stress and increasing BDNF levels.

Sickness Behavior Score Is Associated with Neuroinflammation and Late Behavioral Changes in Polymicrobial Sepsis Animal Model.

The use of reliable scores is a constant development in critical illness. According to Sepsis-3 consensus, the use of Sequential Organ Failure Assessment (SOFA) score of 2 or more is associated with a higher mortality of sepsis patients. In experimental research, due murine animal model limitations, the use of a score systems can be an alternative to assess sepsis severity. In this work, we suggest a sickness behavior score (SBS) that uses physiological variables to assess sepsis severity and mortality. Animals were evaluated daily by the presence of six indicators of sickness behavior: temperature alteration, preference of water/sucrose, liquid intake, food intake, body weight, and movimentation. Male adult Wistar rats were evaluated daily after sepsis induction by cecal ligation and puncture (CLP) or laparotomy only (sham) for determination of SBS. Oxidative stress, IL-6, and HPA axis markers (corticosterone and adrenal gland weight) were evaluated 24 h after CLP to determine the correlation with the acute SBS and neuroinflammation. Also, BDNF and four cognitive behavioral tests were correlated with the chronic SBS, i.e., sum of 8 days after surgery. In result, septic rats presented higher SBS than sham animals. Sepsis severity markers were associated with acute and chronic SBS. Also, SBS was negative correlated with the cognitive tests. In conclusion, SBS shows to be reliable score to predict sepsis severity and mortality. The use of score system provides the analysis of global sickness behavior, beyond evaluation of each parameter individually.

Fish oil-rich lipid emulsion modulates neuroinflammation and prevents long-term cognitive dysfunction after sepsis.

OBJECTIVES: Sepsis is a severe organic dysfunction caused by an infection that affects the normal regulation of several organ systems, including the central nervous system. Inflammation and oxidative stress play crucial roles in the development of brain dysfunction in sepsis. The aim of this study was to determine the effect of a fish oil (FO)-55-enriched lipid emulsion as an important anti-inflammatory compound on brain dysfunction in septic rats. METHODS: Wistar rats were subjected to sepsis by cecal ligation and perforation (CLP) or sham (control) and treated orally with FO (600 µL/kg after CLP) or vehicle (saline; sal). Animals were divided into sham+sal, sham+FO, CLP+sal and CLP+FO groups. At 24 h and 10 d after surgery, the hippocampus, prefrontal cortex, and total cortex were obtained and assayed for levels of interleukin (IL)-1ß and IL-10, blood-brain barrier permeability, nitrite/nitrate concentration, myeloperoxidase activity, thiobarbituric acid reactive species formation, protein carbonyls, superoxide dismutase and catalase activity, and brain-derived neurotrophic factor levels. Behavioral tasks were performed 10 d after surgery. RESULTS: FO reduced BBB permeability in the prefrontal cortex and total cortex of septic rats, decreased IL-1ß levels and protein carbonylation in all brain structures, and diminished myeloperoxidase activity in the hippocampus and prefrontal cortex. FO enhanced brain-derived neurotrophic factor levels in the hippocampus and prefrontal cortex and prevented cognitive impairment. CONCLUSIONS: FO diminishes the negative effect of polymicrobial sepsis in the rat brain by reducing inflammatory and oxidative stress markers.

Isokinetic performance of ankle evertor and invertor muscles in adolescent female volleyball athletes

This study aimed to analyze the muscular performance of evertor and invertor ankle muscles of adolescent volleyball athletes. Methods: The information provided by database concerning the isokinetic evaluation of ankle muscles from 20 female volleyball players between the ages of 14-17 years old were analyzed. The isokinetic dynamometer was used in a concentric-concentric mode for the ankle evertor and invertor muscles at angular velocities of 60°/s, and 180°/s. Results: Seven athletes had suffered ankle sprains (6 affected the non-dominant limb and all were lateral ankle sprain) in the one year prior to the isokinetic evaluation. The isokinetic results demonstrated that the mean peak torque values for eversion of the non-dominant limb were significantly lower in comparison to the dominant limb at 60°/s. Moreover, although no difference was observed in the average values of the evertor/invertor ratio between the limbs, the ratios were below the values suggested by the literature. Conclusion: We believe that although the athletes reported no ankle injury and no pain in the previous assessment month, the isokinetic results of the non-dominant limb seemed to be directly related to previous ankle sprains injuries in inversion of this limb. The athletes had similar results to those of subjects clinically diagnosed with ankle joint instabilities despite their being active in sports at the moment of the assessment.(AU)

Análise da marcha em pacientes portadores de doença pulmonar obstrutiva crônica antes e após programa de reabilitação pulmonar / Chronic obstructive pulmonary disease patients gait analysis before and after a pulmonary rehabilitation program

Introdução: A Doença Pulmonar Obstrutiva Crônica (DPOC) é uma patologia respiratória que está relacionada à diminuição funcional, e pode provocar alterações da marcha em indivíduos com DPOC. Objetivo: Analisar, através de informações de um banco de dados, os parâmetros da cinemática linear e cinemática angular da marcha em pacientes com DPOC antes e após a participação em um Programa de Reabilitação Pulmonar. Métodos: Foram selecionadas avaliações da marcha de 16 sujeitos com DPOC ingressantes no Programa de Reabilitação Pulmonar da Universidade de Caxias do Sul (UCS). A análise cinemática da marcha foi realizada antes e após 12 semanas de participação neste programa. Resultados: Com relação às variáveis lineares da marcha, o comprimento do passo direito (p = 0,003), a cadência (p = 0,003), o tempo da passada direito e esquerdo (p = 0,003 e p = 0,006) e a velocidade (p = 0,013) apresentaram diferenças estatisticamente significativas. Já os aspectos angulares não apresentaram diferenças estatisticamente significativas entre as duas avaliações. Conclusão: O Programa de Reabilitação Pulmonar influenciou positivamente quase todos os parâmetros lineares da marcha, demonstrando a importância da interferência na marcha através de um tratamento adequado para a manutenção ou melhora da qualidade de vida destes indivíduos. (AU)

Introduction: The Chronic Obstructive Pulmonary Disease (COPD) is a respiratory disease that is related to functional impairment, and can cause gait modifications in these individuals. Objective: Analyze, from a database's information, the gait linear and angular kinematics parameters in COPD patients before and after participation in a pulmonary rehabilitation program. Methods: We selected gait analyses of 16 patients with COPD entrants in the University of Caxias do Sul Pulmonary Rehabilitation Program. The cinematic gait analysis was made before and after 12 weeks of this program. Results: About the gait linear parameters, the right step length (p = 0.003), the rhythm (p = 0.003, the right and left times length (p = 0.003 and p = 0.006) and the speed (p = 0.013) showed statistically significant differences. However, the angular aspects did not show statistically significant difference among the two evaluations. Conclusion: The pulmonary rehabilitation program positive influenced almost all the gait linear parameters, showing the importance of gait interference through appropriate treatment to maintenance or improvement of quality of life. (AU)